FastQCFastQC Report
Sun 1 Jan 2017
ERR1142637_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142637_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495231
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14340.289561840837912No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8930.18031989112151703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.041.5238041
GTATCAA23250.033.3252751
TATCAAC29500.026.0548742
ATCAACG29350.025.3760013
CAACGCA29550.025.0000845
TCAACGC30400.024.4995274
AACGCAG31400.023.9041986
ACGCAGA33500.022.0500777
CATGGGT4201.8189894E-1221.279594
CGCAGAG35050.021.0749688
CATGGGG27150.020.4095954
GTACATG45650.019.845231
CAGAGTA35700.019.68806610-11
GCAGAGT36300.019.5287119
CTTAATA1856.882858E-419.3241672
TACATGG47000.019.1425742
ACATGGG47000.019.0158023
AGAGTAC34150.019.0118810-11
ATGGGGG18000.018.8659925
ATGGGTA1959.812051E-418.3313275
Produced by FastQC (version 0.11.5)