FastQCFastQC Report
Sun 1 Jan 2017
ERR1142637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495231
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25240.5096611480299094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24530.4953244041669443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.2938022862058312No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7280.14700210608786607No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.10237646673976386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.053.9167021
GTATCAA28800.037.5970881
TCTAACG902.3952198E-433.045712
TATCAAC37350.029.6216982
ATCAACG36950.029.2984433
CAACGCA37950.028.5264155
CTAACGC1055.911153E-428.3248983
TCAACGC38550.028.2367234
AACGCAG40350.026.9770936
GAGTACT19100.025.22546812-13
ACGCAGA43900.024.931077
CGCAGAG45750.024.0529448
CCGCAGA3553.6379788E-1223.462541
GCAGAGT47550.023.392619
CAGAGTA48000.022.42977510-11
GTACTTT21900.022.13609914-15
AGTACTT20100.021.75098412-13
GTCTAAC1703.9318137E-420.9979861
AGAGTAC46850.020.88545210-11
CATGGGG21600.020.653574
Produced by FastQC (version 0.11.5)