FastQCFastQC Report
Sun 1 Jan 2017
ERR1142636_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142636_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12294
Sequences flagged as poor quality0
Sequence length125
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.5449812916870017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.4555067512607776No Hit
GCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGA320.2602895721490158No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN220.17894908085244834No Hit
GTATCAACGCAGAGTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCA220.17894908085244834No Hit
GGTATCAACGCAGAGTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTC210.1708150317227916No Hit
GTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCACTTTTTCGAACTG210.1708150317227916No Hit
TATCAACGCAGAGTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCAC180.14641288433382138No Hit
GGTATCAACGCAGAGTACATGGGACTGCTCCTCGCCCTTGCTCACTTTTT170.13827883520416462No Hit
CTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGAG160.1301447860745079No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC150.12201073694485115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.12201073694485115No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA140.1138766878151944No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT140.1138766878151944No Hit
GTGTTTCCCCCTCCCATCTTCATCACAAAAGCCCATGTACTCTGCGTTGA140.1138766878151944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1138766878151944No Hit
GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC130.10574263868553767No Hit
GTATCAACGCAGAGTACATGGGACTGCTCCTCGCCCTTGCTCACTTTTTC130.10574263868553767No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAC251.6581671E-595.21
GTAAAAA401.8670416E-674.3751
GTACAAA350.007614474751.01
GTACTTT355.296776E-542.514-15
GAACAAA600.001258518939.6666681
AACATGG401.1635798E-437.187514-15
CAACACA1051.3678871E-534.04
GAGTACT350.002736306334.012-13
TACTTTT400.00526163629.74999814-15
TTGTGAT400.00526163629.74999826-27
ACAACAC800.005160179529.7499983
AACGCAG2403.3833203E-1027.2708327
GCTTTTG557.535648E-427.04545622-23
TTTTGTG450.009351511526.44444724-25
TGGGAGG450.009351511526.44444738-39
GATGAAG600.001252251624.79166630-31
CGCAGAG2251.6120794E-723.8000019
TTTTTCG751.6219068E-423.838-39
GATGGGA650.001995747222.88461536-37
ATGGGAC650.001995747222.88461518-19
Produced by FastQC (version 0.11.5)