FastQCFastQC Report
Sun 1 Jan 2017
ERR1142636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12294
Sequences flagged as poor quality0
Sequence length125
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5194.2215714982918495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4673.7986009435496992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3422.781844802342606No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1781.4478607450789003No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.6995282251504799No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.6913941760208231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC430.3497641125752399No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.3416300634455832No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.27655767040832924No Hit
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACGCTAC320.2602895721490158No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.252155523019359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC310.252155523019359No Hit
TCTCATCCCTGCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTAT310.252155523019359No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.2440214738897023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG280.2277533756303888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA250.20335122824141857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC240.19521717911176184No Hit
GTATCAACGCAGAGTACATGGGCCATCTCATCCCTGCACATCTCCGAGCC230.18708312998210508No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT220.17894908085244834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG220.17894908085244834No Hit
GGTATCAACGCAGAGTACATGGGCCATCTCATCCCTGCACATCTCCGAGC210.1708150317227916No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.16268098259313485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA190.15454693346347811No Hit
TCCCTGCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT190.15454693346347811No Hit
GCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGA180.14641288433382138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC170.13827883520416462No Hit
GGTATCAACGCAGAGTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTC160.1301447860745079No Hit
TATCAACGCAGAGTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCC160.1301447860745079No Hit
CTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGAG160.1301447860745079No Hit
CTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.12201073694485115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT150.12201073694485115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT150.12201073694485115No Hit
AGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGAGCTAGC150.12201073694485115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC140.1138766878151944No Hit
GCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT140.1138766878151944RNA PCR Primer, Index 31 (95% over 24bp)
GTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCACTTTTTCGAACTG140.1138766878151944No Hit
ACGCAGAGTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCACTTTTT140.1138766878151944No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA130.10574263868553767No Hit
GGTATCAACGCAGAGTACATGGGACTGCTGCTACTGCTCCTCGCCCTTGC130.10574263868553767No Hit
GTATCAACGCAGAGTACATGGGACTGCTCCTCGCCCTTGCTCACTTTTTC130.10574263868553767No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC130.10574263868553767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2050.063.772631
TGGTATC901.4315447E-952.821772
GTGGTAT901.4315447E-952.821771
GTATCAA3450.048.228571
TATCAAC4450.037.390692
ATCAACG4550.036.5689163
TCAACGC4750.035.029174
CAACGCA4850.034.306925
AACGCAG5050.032.948236
ACGCAGA5350.031.1006687
CGCAGAG5550.029.9799238
GCAGAGT5950.027.9644669
GGGACTG450.00940959226.41088518-19
AGAGTAC6050.025.04668810-11
CAGAGTA6050.024.55557610-11
GAGTACA1751.8189894E-1223.76979612-13
ACATGGG1803.6379788E-1223.10952416-17
AGTACTT5100.022.13853512-13
CATGGGA1103.9520364E-621.60890616-17
AGTACAT1808.0035534E-1121.45884312-13
Produced by FastQC (version 0.11.5)