FastQCFastQC Report
Sun 1 Jan 2017
ERR1142632_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142632_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732897
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12640.17246625378463823No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11260.15363686848220146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.034.422211
GTATCAA31200.028.8385491
TATCAAC37600.023.9216612
CAACGCA36900.023.891185
ATCAACG37150.023.8907453
AACGCAG38200.023.7018626
ACCGTCC1552.0913096E-423.054858
TCAACGC39200.022.6413574
CATGGGG30850.020.6600254
ACGCAGA43050.020.6136957
ATGGGGG16650.020.0343865
CGCAGAG44450.019.8304568
GTACATG56150.018.9956551
TACATGG58000.018.4861622
ACATGGG55600.018.3199183
TTAGGCC1650.00811073418.0479879
CTAGTAC2702.42186E-517.649343
CAGAGTA46500.017.61013810-11
GTACTAG2050.001371598517.4400771
GCAGAGT48600.017.4018259
Produced by FastQC (version 0.11.5)