FastQCFastQC Report
Sun 1 Jan 2017
ERR1142632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732897
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.44221766496519976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.4182033764635412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.23877843680626337No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8550.11666032198248869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30500.050.513711
GTATCAA42500.036.103662
ATCAACG53850.028.8253924
TATCAAC54000.028.7453233
GTACTAG2302.6557245E-1028.4494721
CAACGCA54850.028.1895126
TCAACGC56050.027.587875
AACGCAG57050.027.2066887
ACGCAGA62050.024.8226858
GAGTACT28350.024.7525412-13
CGCAGAG65450.023.624069
CAGAGTA68250.022.611310-11
GCAGAGT68500.022.3117379
GTACTTT31700.022.23053414-15
CTCAACG4550.022.225261
CATGGGG31050.022.2186074
GTACATG56100.021.2068651
TACATGG57150.021.0211072
ACATGGG55700.020.8209173
GTATCGT1450.00387161120.5121571
Produced by FastQC (version 0.11.5)