##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142632_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732897
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.74497098500881	27.0	14.0	33.0	14.0	33.0
2	28.126326073104405	33.0	27.0	33.0	14.0	33.0
3	31.329016219195875	33.0	33.0	33.0	27.0	33.0
4	30.936106983655275	33.0	33.0	33.0	27.0	33.0
5	32.1516406807505	33.0	33.0	33.0	33.0	33.0
6	34.96851945089146	37.0	37.0	37.0	27.0	37.0
7	35.14520867188705	37.0	37.0	37.0	33.0	37.0
8	35.51029407952277	37.0	37.0	37.0	33.0	37.0
9	35.915363277513755	37.0	37.0	37.0	33.0	37.0
10-11	35.97298665433206	37.0	37.0	37.0	33.0	37.0
12-13	36.07826747824046	37.0	37.0	37.0	37.0	37.0
14-15	36.041524934608816	37.0	37.0	37.0	35.0	37.0
16-17	36.08078966075724	37.0	37.0	37.0	37.0	37.0
18-19	36.085170221736476	37.0	37.0	37.0	37.0	37.0
20-21	36.09128772528746	37.0	37.0	37.0	37.0	37.0
22-23	36.08599639512783	37.0	37.0	37.0	37.0	37.0
24-25	36.07615804130731	37.0	37.0	37.0	37.0	37.0
26-27	36.0353098730108	37.0	37.0	37.0	37.0	37.0
28-29	36.013561250762386	37.0	37.0	37.0	37.0	37.0
30-31	35.997487368620696	37.0	37.0	37.0	35.0	37.0
32-33	35.94140104271132	37.0	37.0	37.0	33.0	37.0
34-35	35.83689113204174	37.0	37.0	37.0	33.0	37.0
36-37	35.76705799041339	37.0	37.0	37.0	33.0	37.0
38-39	35.7630403726581	37.0	37.0	37.0	33.0	37.0
40-41	35.667642247137046	37.0	37.0	37.0	33.0	37.0
42-43	35.63673817739737	37.0	37.0	37.0	33.0	37.0
44-45	35.55785737968637	37.0	37.0	37.0	33.0	37.0
46-47	35.44855484467804	37.0	37.0	37.0	33.0	37.0
48-49	35.30238764792324	37.0	37.0	37.0	33.0	37.0
50-51	35.2153317587601	37.0	37.0	37.0	33.0	37.0
52-53	35.1124707837527	37.0	37.0	37.0	33.0	37.0
54-55	35.05004523145817	37.0	37.0	37.0	33.0	37.0
56-57	35.03690218407225	37.0	37.0	37.0	33.0	37.0
58-59	34.99232088547231	37.0	37.0	37.0	27.0	37.0
60-61	34.94253967474283	37.0	37.0	37.0	27.0	37.0
62-63	34.91750955454859	37.0	37.0	37.0	27.0	37.0
64-65	34.88428933397189	37.0	37.0	37.0	27.0	37.0
66-67	34.852589108701494	37.0	37.0	37.0	27.0	37.0
68-69	34.82577292579994	37.0	37.0	37.0	27.0	37.0
70-71	34.70521301083235	37.0	37.0	37.0	27.0	37.0
72-73	34.70266967936831	37.0	37.0	37.0	27.0	37.0
74-75	34.616872493679196	37.0	37.0	37.0	27.0	37.0
76-77	34.4952148801264	37.0	37.0	37.0	27.0	37.0
78-79	34.49799426113083	37.0	37.0	37.0	27.0	37.0
80-81	34.48280590587763	37.0	37.0	37.0	27.0	37.0
82-83	34.397467857011286	37.0	37.0	37.0	27.0	37.0
84-85	34.34405584959414	37.0	37.0	37.0	27.0	37.0
86-87	34.19906480719665	37.0	37.0	37.0	27.0	37.0
88-89	34.117465346426584	37.0	37.0	37.0	27.0	37.0
90-91	34.05387591980865	37.0	37.0	37.0	27.0	37.0
92-93	33.86587883427003	37.0	35.0	37.0	24.5	37.0
94-95	33.914532328553676	37.0	33.0	37.0	27.0	37.0
96-97	33.91607415503134	37.0	35.0	37.0	22.0	37.0
98-99	33.86885401359264	37.0	33.0	37.0	22.0	37.0
100-101	33.74015652949869	37.0	33.0	37.0	22.0	37.0
102-103	33.66604516050687	37.0	33.0	37.0	22.0	37.0
104-105	33.50733322690637	37.0	33.0	37.0	22.0	37.0
106-107	33.46715227378472	37.0	33.0	37.0	22.0	37.0
108-109	33.34157323607546	37.0	33.0	37.0	22.0	37.0
110-111	33.25411688136259	37.0	33.0	37.0	18.0	37.0
112-113	33.10041247269398	37.0	33.0	37.0	14.0	37.0
114-115	33.07307575280019	37.0	33.0	37.0	14.0	37.0
116-117	32.91710704232655	37.0	33.0	37.0	14.0	37.0
118-119	32.80020657745904	37.0	33.0	37.0	14.0	37.0
120-121	32.69246156008279	37.0	33.0	37.0	14.0	37.0
122-123	32.625115807541846	37.0	33.0	37.0	14.0	37.0
124-125	31.149306792086747	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	14.0
16	10.0
17	21.0
18	89.0
19	426.0
20	1403.0
21	3292.0
22	5225.0
23	7530.0
24	6753.0
25	5560.0
26	5730.0
27	6970.0
28	9292.0
29	12284.0
30	16591.0
31	22960.0
32	32559.0
33	48211.0
34	79501.0
35	162106.0
36	306370.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.18787472652556	14.567060779226452	12.487767861651616	28.75729663259638
2	18.16776436525187	20.091363452163126	37.095526383652825	24.645345798932183
3	20.686427542243376	24.931777496397853	28.63585010697289	25.74594485438589
4	15.148753158719156	18.361504832908533	32.85518193676558	33.63456007160673
5	17.159940943538857	34.072903618436314	31.86524538177928	16.901910056245548
6	30.974238620237966	33.37326711057745	18.276116144525705	17.37637812465888
7	27.051959552297255	31.084859127544522	22.004183398212845	19.858997921945377
8	26.618610800699145	31.339874498053614	20.674937951717638	21.366576749529607
9	26.821777139216014	15.785983569314652	20.42660837743912	36.965630914030214
10-11	26.08415643671621	24.623582849977556	26.07214929246538	23.220111420840855
12-13	27.260757324367983	23.516706326682094	25.930418504126095	23.292117844823824
14-15	25.22284850395076	23.924780699061397	24.687916583094214	26.164454213893627
16-17	24.307849534109156	25.83241574191189	25.60332488739891	24.256409836580037
18-19	24.04062235211769	24.58442318634133	27.884955184698534	23.48999927684245
20-21	25.5173528888052	23.47236520648747	27.756230645956858	23.254051258750465
22-23	25.343056391280083	23.40499415333942	27.544116021760217	23.707833433620277
24-25	24.412366267019785	24.141250407628903	27.59309971251076	23.85328361284055
26-27	24.10979987638099	24.26664319815745	27.709760034493254	23.913796890968307
28-29	23.917872517516393	24.08904534817877	28.066749899370297	23.926332234934538
30-31	24.18255796183906	23.854953499541544	28.08236093524866	23.880127603370738
32-33	24.050138082347292	24.002586997336593	27.734971946906516	24.212302973409596
34-35	24.250149918849388	24.04043408584978	27.732497016973067	23.97691897832777
36-37	24.244661153280948	24.161496446226753	27.551805677031666	24.042036723460633
38-39	24.146094198775543	24.22748353452122	27.523035296910752	24.10338696979248
40-41	24.00432266924826	24.153596664121913	27.77615177372027	24.065928892909554
42-43	24.20313159849881	24.1085055012079	27.591318959535915	24.097043940757377
44-45	24.286086585154532	24.25531827801178	27.256558561434964	24.202036575398726
46-47	24.38760153200245	24.240104680466697	27.169711432847997	24.202582354682857
48-49	24.41768761504004	24.471515096937225	26.889180880805895	24.22161640721684
50-51	24.118259455284985	24.80948891863386	26.792919059567716	24.27933256651344
52-53	24.213566162775944	24.655510938099077	26.63532529127558	24.495597607849398
54-55	24.209336373323946	24.628631308355743	26.653267785241308	24.508764533079
56-57	24.37334304820459	24.52493324437131	26.38215192585043	24.719571781573674
58-59	24.130607711588397	24.600591897633635	26.598962746470512	24.669837644307453
60-61	24.24406158027663	24.563410683902376	26.839173853897613	24.353353881923383
62-63	23.982087523894897	24.709133752764714	26.819935134132084	24.488843589208305
64-65	23.982428635947482	24.43262832294306	26.88010730020726	24.7048357409022
66-67	24.247199811160367	24.350897875144803	26.80485798140803	24.597044332286803
68-69	24.129243263378072	24.433651659100803	26.821640694394983	24.61546438312614
70-71	23.930852493597328	24.424919190554746	27.005568313146323	24.638660002701606
72-73	24.15728267410018	24.37245615686788	27.1060599238365	24.364201245195435
74-75	24.11576638359084	24.421007644344435	27.183608319002033	24.279617653062687
76-77	24.058381121598202	24.322371736479187	27.068324813615526	24.550922328307088
78-79	23.96236841566664	24.409567536959592	27.117868180298675	24.510195867075094
80-81	23.69807762891648	24.58571941214113	27.260856573297477	24.455346385644912
82-83	23.934604726175714	24.460531288844134	27.202731079537777	24.402132905442375
84-85	23.962592499793477	24.51518467870936	26.932553578024116	24.589669243473043
86-87	23.823597343723733	24.66799963373017	27.02852667961367	24.479876342932428
88-89	23.92967197022932	24.275214708158227	27.051929160662674	24.743184160949774
90-91	24.07064999194304	24.43915355475866	26.929300846936854	24.56089560636145
92-93	24.003426660181333	24.59045399148527	26.90358624307973	24.502533105253665
94-95	23.673045291213356	24.4979979956078	27.033506369641852	24.795450343536995
96-97	23.76670937389565	24.422122071723585	26.938232793966954	24.87293576041381
98-99	23.73300750310071	24.550789537956902	26.7296086626088	24.986594296333593
100-101	23.877639013394788	24.81385515290689	26.44089142130477	24.867614412393557
102-103	23.889305045593037	24.666494746192168	26.68226230971064	24.761937898504154
104-105	23.86087331454005	24.843086100703246	26.480857868754487	24.815182716002216
106-107	23.83936624109527	24.7302827000247	26.472546619784225	24.957804439095806
108-109	23.9390689513081	24.89841641492494	26.17629561710152	24.98621901666544
110-111	23.678786840979594	25.07059318744188	26.26441796351872	24.986202008059802
112-113	23.774434725776423	25.085397460623703	26.055043242804405	25.085124570795468
114-115	23.657826406711994	25.064572511553468	26.17455113065001	25.103049951084532
116-117	23.759081286597183	25.03620916789524	26.06623463540796	25.13847491009961
118-119	23.617045517341126	24.941857410971398	26.190669487437855	25.250427584249618
120-121	23.5537350771903	24.864220254838166	26.379986805776806	25.20205786219473
122-123	23.48890771827419	25.10127616841111	26.374374571051597	25.035441542263104
124-125	23.627007127964983	24.946308303587998	26.177055702917773	25.24962886552925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	166.0
1	182.5
2	379.0
3	502.5
4	352.0
5	311.0
6	457.0
7	947.5
8	1591.5
9	1927.0
10	1759.5
11	1347.0
12	1170.0
13	1140.5
14	1114.5
15	1070.5
16	1031.5
17	1012.0
18	977.0
19	939.5
20	932.0
21	908.0
22	896.5
23	930.0
24	971.0
25	1089.5
26	1289.5
27	1508.0
28	1816.0
29	2381.5
30	3265.0
31	4107.0
32	5036.0
33	6110.5
34	7138.0
35	8364.0
36	9656.0
37	11185.5
38	12727.0
39	13864.0
40	14627.5
41	15368.0
42	16595.5
43	18472.5
44	20756.0
45	23165.5
46	25579.5
47	27609.5
48	29747.0
49	31853.0
50	32505.5
51	31892.0
52	31437.0
53	31812.0
54	31719.0
55	30571.0
56	29145.5
57	26843.0
58	24821.5
59	22661.5
60	19590.5
61	16536.0
62	13528.0
63	10778.0
64	8355.5
65	6580.5
66	5111.0
67	3928.0
68	3218.0
69	2693.5
70	2280.0
71	1972.5
72	1733.0
73	1613.0
74	1334.0
75	1067.0
76	875.5
77	636.5
78	470.5
79	317.5
80	210.5
81	151.0
82	107.0
83	69.0
84	45.5
85	25.5
86	11.5
87	3.0
88	0.5
89	1.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0267431849223015
2	0.0
3	1.364448210321505E-4
4	6.822241051607524E-4
5	0.005321348020253869
6	0.002319561957546558
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.364448210321505E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0010233361577411287
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0016373378523858058
30-31	1.364448210321505E-4
32-33	1.364448210321505E-4
34-35	6.822241051607525E-5
36-37	0.001910227494450107
38-39	0.0
40-41	0.002251339547030483
42-43	0.001705560262901881
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07170175345239509
76-77	0.09196380937566943
78-79	2.72889642064301E-4
80-81	0.0
82-83	0.0
84-85	0.07238397755755584
86-87	0.163188005954452
88-89	0.19634409746526457
90-91	0.08336778565064396
92-93	0.13494392800079685
94-95	8.868913367089783E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0015008930313536555
106-107	0.0
108-109	4.0933446309645145E-4
110-111	6.822241051607525E-5
112-113	6.822241051607525E-5
114-115	0.0
116-117	3.411120525803762E-4
118-119	6.822241051607525E-5
120-121	6.822241051607525E-5
122-123	0.0
124-125	0.0012280033892893545
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	732897.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.76491032982477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.38357536280256	65.44843841332397
2	9.44696394896997	14.314967529655942
3	2.09948020531791	4.772007884854607
4	0.7589122535052218	2.299956753401135
5	0.38858436296821824	1.4720529707929568
6	0.23739586746625171	1.0791765966750915
7	0.14659875996499303	0.7774929332247844
8	0.10243600142523115	0.6208843570022753
9	0.06297303438731926	0.4294031673196995
>10	0.3367032150610788	4.516124835592225
>50	0.023892636413030757	1.226220749917341
>100	0.011192867057780638	1.4062563661629661
>500	6.457423302198062E-4	0.3232285883837882
>1k	6.457423302198062E-4	1.313788853693137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3241	0.44221766496519976	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3065	0.4182033764635412	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1750	0.23877843680626337	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	855	0.11666032198248869	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	2.0466723154822575E-4
14-15	0.0	0.0	0.0	0.0	2.72889642064301E-4
16-17	0.0	0.0	0.0	0.0	2.72889642064301E-4
18-19	0.0	0.0	0.0	1.364448210321505E-4	2.72889642064301E-4
20-21	0.0	0.0	0.0	4.775568736125267E-4	2.72889642064301E-4
22-23	0.0	0.0	0.0	0.0017737826734179564	3.411120525803762E-4
24-25	0.0	0.0	0.0	0.003956899809932364	4.0933446309645145E-4
26-27	0.0	0.0	0.0	0.006617573820059299	4.0933446309645145E-4
28-29	0.0	0.0	0.0	0.012552923534957846	4.0933446309645145E-4
30-31	0.0	0.0	0.0	0.025651626354044294	4.775568736125267E-4
32-33	0.0	0.0	0.0	0.04864257869796165	5.45779284128602E-4
34-35	0.0	0.0	0.0	0.07395309299942557	6.822241051607524E-4
36-37	0.0	0.0	0.0	0.11208942047791162	6.822241051607524E-4
38-39	0.0	0.0	0.0	0.16823646433264156	6.822241051607524E-4
40-41	0.0	0.0	0.0	0.22977307861814145	6.822241051607524E-4
42-43	0.0	0.0	0.0	0.27930254865281207	6.822241051607524E-4
44-45	0.0	0.0	0.0	0.3317655823396739	7.504465156768277E-4
46-47	0.0	0.0	0.0	0.4051046736444548	0.0010233361577411287
48-49	0.0	0.0	0.0	0.4795353235174929	0.0013644482103215049
50-51	0.0	0.0	0.0	0.5470072875178913	0.0013644482103215049
52-53	0.0	0.0	0.0	0.6077934552877144	0.0014326706208375802
54-55	0.0	0.0	0.0	0.6660553938684426	0.0015008930313536555
56-57	0.0	0.0	0.0	0.7303209045745855	0.0015008930313536555
58-59	0.0	0.0	0.0	0.8080944525629112	0.0015008930313536555
60-61	0.0	0.0	0.0	0.8832755489516262	0.0015008930313536555
62-63	0.0	0.0	0.0	0.9524530732149266	0.001705560262901881
64-65	0.0	0.0	0.0	1.0242912714883539	0.0017737826734179564
66-67	0.0	0.0	0.0	1.1031563780449367	0.0020466723154822573
68-69	0.0	0.0	0.0	1.1951884098311223	0.002183117136514408
70-71	0.0	0.0	0.0	1.2895400035748543	0.0023195619575465585
72-73	0.0	0.0	0.0	1.3838915973185864	0.0023195619575465585
74-75	0.0	0.0	0.0	1.470602281084518	0.0023195619575465585
76-77	0.0	0.0	0.0	1.5625660904601875	0.0023195619575465585
78-79	0.0	0.0	0.0	1.6711761680017791	0.0023195619575465585
80-81	0.0	0.0	0.0	1.7846300366900123	0.0023195619575465585
82-83	0.0	0.0	0.0	1.8988343518939224	0.0023195619575465585
84-85	0.0	0.0	0.0	2.0081266535406748	0.0023195619575465585
86-87	0.0	0.0	0.0	2.1208982981237474	0.0023195619575465585
88-89	0.0	0.0	0.0	2.2487470954308724	0.0023195619575465585
90-91	0.0	0.0	0.0	2.382531242452896	0.0023195619575465585
92-93	0.0	0.0	0.0	2.520545178926916	0.0023195619575465585
94-95	0.0	0.0	0.0	2.6559666638013253	0.0023195619575465585
96-97	0.0	0.0	0.0	2.7858621334239326	0.0023877843680626338
98-99	0.0	0.0	0.0	2.938202776106329	0.002456006778578709
100-101	0.0	0.0	0.0	3.096819880556204	0.002456006778578709
102-103	0.0	0.0	0.0	3.270173025677551	0.0026606740101269345
104-105	0.0	0.0	0.0	3.4399786054520622	0.0027288964206430098
106-107	0.0	0.0	0.0	3.6101252972791538	0.0027288964206430098
108-109	0.0	0.0	0.0	3.785184002663403	0.0028653412416751603
110-111	0.0	0.0	0.0	3.9691116214147417	0.0029335636521912356
112-113	0.0	0.0	0.0	4.167297723963941	0.003001786062707311
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3050	0.0	50.51371	1
GTATCAA	4250	0.0	36.10366	2
ATCAACG	5385	0.0	28.825392	4
TATCAAC	5400	0.0	28.745323	3
GTACTAG	230	2.6557245E-10	28.449472	1
CAACGCA	5485	0.0	28.189512	6
TCAACGC	5605	0.0	27.58787	5
AACGCAG	5705	0.0	27.206688	7
ACGCAGA	6205	0.0	24.822685	8
GAGTACT	2835	0.0	24.75254	12-13
CGCAGAG	6545	0.0	23.62406	9
CAGAGTA	6825	0.0	22.6113	10-11
GCAGAGT	6850	0.0	22.311737	9
GTACTTT	3170	0.0	22.230534	14-15
CTCAACG	455	0.0	22.22526	1
CATGGGG	3105	0.0	22.218607	4
GTACATG	5610	0.0	21.206865	1
TACATGG	5715	0.0	21.021107	2
ACATGGG	5570	0.0	20.820917	3
GTATCGT	145	0.003871611	20.512157	1
>>END_MODULE