FastQCFastQC Report
Sun 1 Jan 2017
ERR1142631_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142631_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803095
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22930.2855203929796599No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13180.16411507978508147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.039.293471
GTATCAA40900.033.6412241
TATCAAC49750.027.2959232
ATCAACG49850.026.8827293
CAACGCA49650.026.7494285
TCAACGC50700.026.3145564
AACGCAG50600.026.2439336
ACGCAGA55450.023.6277757
CGCAGAG57000.022.7748858
GCAGAGT59800.021.7085049
CATGGGG40900.020.9698454
AGAGTAC56650.020.28766310-11
GTACATG74900.020.2787651
CAGAGTA59150.020.18525310-11
TACATGG76900.019.3628672
ACATGGG74250.019.1715583
ATGGGGC11100.018.7790645
GAGTACA63550.018.45914812-13
AGTAGCG1650.008112892518.0474115
AGTACAT63950.017.69188512-13
Produced by FastQC (version 0.11.5)