##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142631_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	803095
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36924398732404	33.0	33.0	33.0	27.0	33.0
2	31.410565375204676	33.0	33.0	33.0	27.0	33.0
3	31.51380471799725	33.0	33.0	33.0	27.0	33.0
4	31.440837011810558	33.0	33.0	33.0	27.0	33.0
5	31.46841407305487	33.0	33.0	33.0	27.0	33.0
6	34.746784626974396	37.0	37.0	37.0	27.0	37.0
7	34.84434469147486	37.0	37.0	37.0	27.0	37.0
8	34.87895578978826	37.0	37.0	37.0	33.0	37.0
9	34.75554822281299	37.0	37.0	37.0	27.0	37.0
10-11	34.805052328802944	37.0	37.0	37.0	27.0	37.0
12-13	34.819779727180475	37.0	37.0	37.0	27.0	37.0
14-15	34.775710221082186	37.0	37.0	37.0	27.0	37.0
16-17	34.78219201962408	37.0	37.0	37.0	27.0	37.0
18-19	34.792502132375375	37.0	37.0	37.0	27.0	37.0
20-21	34.8540041962657	37.0	37.0	37.0	27.0	37.0
22-23	34.913257460200846	37.0	37.0	37.0	27.0	37.0
24-25	34.96345139740629	37.0	37.0	37.0	27.0	37.0
26-27	34.95133265678407	37.0	37.0	37.0	27.0	37.0
28-29	35.00068049234524	37.0	37.0	37.0	27.0	37.0
30-31	35.067046862450894	37.0	37.0	37.0	33.0	37.0
32-33	35.08075570138028	37.0	37.0	37.0	33.0	37.0
34-35	35.07976080040344	37.0	37.0	37.0	33.0	37.0
36-37	35.02637545993936	37.0	37.0	37.0	30.0	37.0
38-39	35.02426798822057	37.0	37.0	37.0	27.0	37.0
40-41	35.00540409291553	37.0	37.0	37.0	27.0	37.0
42-43	34.99927779403433	37.0	37.0	37.0	27.0	37.0
44-45	35.021276436785186	37.0	37.0	37.0	30.0	37.0
46-47	35.01702102490988	37.0	37.0	37.0	27.0	37.0
48-49	34.99748099539905	37.0	37.0	37.0	27.0	37.0
50-51	34.98798460954184	37.0	37.0	37.0	27.0	37.0
52-53	34.965460499691815	37.0	37.0	37.0	27.0	37.0
54-55	34.932170540222515	37.0	37.0	37.0	27.0	37.0
56-57	34.86612169170522	37.0	37.0	37.0	27.0	37.0
58-59	34.834001581382026	37.0	37.0	37.0	27.0	37.0
60-61	34.807505961312174	37.0	37.0	37.0	27.0	37.0
62-63	34.84792708210112	37.0	37.0	37.0	27.0	37.0
64-65	34.786639189635096	37.0	37.0	37.0	27.0	37.0
66-67	34.77015981919947	37.0	37.0	37.0	27.0	37.0
68-69	34.72809754761268	37.0	37.0	37.0	27.0	37.0
70-71	34.69578941470187	37.0	37.0	37.0	27.0	37.0
72-73	34.66597351496398	37.0	37.0	37.0	27.0	37.0
74-75	34.580845354534645	37.0	37.0	37.0	27.0	37.0
76-77	34.5396478623326	37.0	37.0	37.0	27.0	37.0
78-79	34.473501889564744	37.0	37.0	37.0	27.0	37.0
80-81	34.39794856150269	37.0	37.0	37.0	27.0	37.0
82-83	34.3616645602326	37.0	37.0	37.0	27.0	37.0
84-85	34.34637060372683	37.0	37.0	37.0	27.0	37.0
86-87	34.2933164818608	37.0	37.0	37.0	27.0	37.0
88-89	34.24247754001706	37.0	37.0	37.0	27.0	37.0
90-91	34.16974953150001	37.0	37.0	37.0	27.0	37.0
92-93	34.0415162589731	37.0	37.0	37.0	27.0	37.0
94-95	33.9801735784683	37.0	37.0	37.0	27.0	37.0
96-97	33.88937236566035	37.0	37.0	37.0	22.0	37.0
98-99	33.84714572995723	37.0	35.0	37.0	22.0	37.0
100-101	33.74350668351814	37.0	33.0	37.0	22.0	37.0
102-103	33.67681469813658	37.0	33.0	37.0	22.0	37.0
104-105	33.548054713327815	37.0	33.0	37.0	22.0	37.0
106-107	33.458973720419124	37.0	33.0	37.0	22.0	37.0
108-109	33.34079778855553	37.0	33.0	37.0	22.0	37.0
110-111	33.264321780113185	37.0	33.0	37.0	22.0	37.0
112-113	33.18375659168591	37.0	33.0	37.0	18.0	37.0
114-115	33.01145443565207	37.0	33.0	37.0	14.0	37.0
116-117	32.82758017420106	37.0	33.0	37.0	14.0	37.0
118-119	32.71670163554748	37.0	33.0	37.0	14.0	37.0
120-121	32.634812817910706	37.0	33.0	37.0	14.0	37.0
122-123	32.42659897023391	37.0	33.0	37.0	14.0	37.0
124-125	30.751457797645358	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1332.0
1	128.0
2	47.0
3	41.0
4	19.0
5	25.0
6	28.0
7	29.0
8	21.0
9	22.0
10	16.0
11	31.0
12	19.0
13	22.0
14	50.0
15	257.0
16	382.0
17	710.0
18	1581.0
19	2846.0
20	3923.0
21	4830.0
22	5620.0
23	6224.0
24	7240.0
25	8231.0
26	9933.0
27	11992.0
28	14456.0
29	17348.0
30	21303.0
31	25694.0
32	32700.0
33	43669.0
34	66233.0
35	128332.0
36	387761.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.54270747683872	19.703645574302286	12.509840576414105	24.243806372444887
2	17.703224388300427	22.647153228697142	35.96709792563561	23.68252445736681
3	20.01933149009823	26.910857853605176	28.317386102806562	24.752424553490034
4	14.902010737420246	19.39853962093085	33.44435411257682	32.25509552907208
5	15.65858435465578	37.195914044037195	30.848143133365642	16.297358467941383
6	29.386565456171827	35.41471983049407	18.4144050052221	16.784309708111998
7	26.432128658565535	33.12980551786522	21.21049698768838	19.227568835880867
8	25.73905764918264	33.78195878542561	20.207384642292723	20.271598923099027
9	26.349558185562067	18.562203874372763	19.7454569080986	35.342781031966574
10-11	25.378864783113613	27.27001834743177	25.173088684251134	22.178028185203484
12-13	26.21776987594596	26.190220358655985	24.801499793222444	22.790509972175613
14-15	24.593044574416982	26.33224453743665	23.341647661150226	25.73306322699614
16-17	24.04030212861069	28.268117290232514	23.972499253918645	23.71908132723815
18-19	24.110889828127636	27.375153736179353	25.399651635378362	23.114304800314653
20-21	26.127200174272463	25.952178686702176	24.8544244420878	23.06619669693756
22-23	26.12730472992373	25.729461844782602	24.54410975320509	23.59912367208858
24-25	24.863999940089453	26.417929728673872	24.77575669827988	23.94231363295679
26-27	24.269402203557597	26.73439354734828	24.904856589016664	24.091347660077464
28-29	24.086845609607856	26.457795899213863	25.303677578217336	24.151680912960945
30-31	24.63153139042415	26.14003897633152	25.151091006551596	24.077338626692736
32-33	24.37218148564839	26.501694462592347	24.852346819770457	24.27377723198881
34-35	24.490365227072598	26.490336519649293	25.07786888570888	23.941429367569224
36-37	24.49665644087554	26.55077991418323	24.7681100092107	24.18445363573053
38-39	24.26042886546996	26.497719285220057	25.00563081682095	24.236221032489034
40-41	24.168542502263797	26.399762356020386	25.192758099272528	24.23893704244329
42-43	24.311782346136134	26.397182609954463	25.070540827570888	24.220494216338516
44-45	24.297718458474545	26.520923697385037	24.92881101289263	24.252546831247784
46-47	24.332994102881045	26.456293868830755	24.908417137398317	24.302294890889883
48-49	24.265080236591878	26.625695674960692	24.99712995083481	24.11209413761262
50-51	24.083292050019153	26.823339443679785	24.978879997204796	24.11448850909626
52-53	24.054385695543466	26.587175271784925	24.982492669390446	24.375946363281166
54-55	23.936127390745124	26.436849755082605	25.088789966406154	24.538232887766114
56-57	24.13651287562353	26.374262108166096	24.822185152109157	24.667039864101213
58-59	24.095046484837667	26.474141599967048	25.105806205217235	24.325005709978047
60-61	24.2172013481463	26.45387592060916	25.235925602296845	24.092997128947697
62-63	23.904768323336402	26.402565504227166	25.22546134395679	24.467204828479645
64-65	23.87188694717686	26.32245067606277	25.18850250192528	24.61715987483509
66-67	24.329245955942692	26.16422857278367	25.065947418050154	24.440578053223483
68-69	24.195959893468586	26.185124540414794	24.960936817486328	24.657978748630292
70-71	24.105118715332182	26.21172382936742	25.11995087770533	24.563206577595068
72-73	24.34284052638903	26.299732736947135	25.106689938042148	24.250736798621688
74-75	24.276912037686994	26.251984962640584	25.30269705336391	24.168405946308518
76-77	24.235524336237628	25.967992641814487	25.214140545586496	24.582342476361394
78-79	24.11531637678021	26.140267559655495	25.250295024553655	24.49412103901064
80-81	24.08844660594012	26.396080354724905	25.226102181837696	24.289370857497282
82-83	24.157340184992297	26.123031547046594	25.481531038726562	24.238097229234548
84-85	24.119503447862776	26.358368086492312	25.122852728965288	24.39927573667962
86-87	23.896952394262154	26.540211325953063	25.29578895355221	24.267047326232568
88-89	24.122166934439782	25.97469934018625	25.277199769143177	24.625933956230796
90-91	24.336990706109813	25.999185217118427	25.259517082054128	24.404306994717636
92-93	24.22526756279637	26.336753153312813	25.20484910962165	24.233130174269167
94-95	23.710009402395706	26.27292652841963	25.49478001700792	24.522284052176747
96-97	23.778256760255406	26.33200971967048	25.321675551410383	24.56805796866373
98-99	23.683933095162793	26.422020981215216	25.239839231765888	24.6542066918561
100-101	23.87973959921932	26.62278001598033	25.10695655807902	24.39052382672133
102-103	23.92855605606074	26.568341414818153	25.267880480110687	24.235222049010417
104-105	23.906298344195704	26.622244068946934	25.3362884632659	24.135169123591467
106-107	23.913058395449184	26.539234822554214	25.343516402763978	24.204190379232628
108-109	23.804132412357937	26.580719775582423	25.269371579637482	24.34577623242215
110-111	23.59443049281481	27.122966045767168	25.095031182204124	24.1875722792139
112-113	23.56979562048349	27.035358623206264	25.019882792885234	24.374962963425016
114-115	23.593822217537234	27.070056741738078	25.02568254441505	24.310438496309636
116-117	23.64170676660774	27.080466452661696	25.00577014967456	24.272056631056003
118-119	23.57884771065161	26.781765044137952	25.147906113452688	24.491481131757748
120-121	23.67435074918713	26.8492974674899	25.316618338384465	24.159733444938503
122-123	23.610741210378954	27.04400907388153	25.302083287853733	24.043166427885783
124-125	23.67304145640339	26.95779516224498	24.99303020494004	24.376133176411592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2560.0
1	1712.5
2	680.5
3	449.0
4	353.5
5	276.5
6	221.5
7	204.0
8	209.5
9	227.5
10	227.0
11	198.0
12	197.0
13	215.5
14	211.0
15	219.5
16	246.0
17	266.5
18	304.5
19	362.0
20	394.0
21	460.5
22	592.5
23	738.5
24	934.5
25	1249.0
26	1620.0
27	2053.5
28	2655.0
29	3646.5
30	5036.5
31	6328.0
32	7678.5
33	9198.0
34	10461.5
35	11919.0
36	13815.5
37	15694.5
38	16928.0
39	17709.0
40	18341.0
41	18951.0
42	20094.5
43	21978.5
44	24006.5
45	26308.5
46	28889.5
47	31038.5
48	32966.5
49	34254.5
50	34399.5
51	34035.5
52	33774.5
53	33846.0
54	33478.5
55	32210.5
56	30820.0
57	28087.0
58	25013.0
59	22309.0
60	19281.0
61	16470.0
62	13663.5
63	10768.0
64	8196.0
65	6350.5
66	4945.0
67	3866.0
68	3107.5
69	2574.0
70	2145.0
71	1821.5
72	1590.5
73	1425.0
74	1153.0
75	921.0
76	760.5
77	587.0
78	427.0
79	307.0
80	241.5
81	177.0
82	125.5
83	86.5
84	51.0
85	26.5
86	18.5
87	11.5
88	4.0
89	3.0
90	2.5
91	2.0
92	1.5
93	1.0
94	1.0
95	1.5
96	1.0
97	0.0
98	0.0
99	0.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35325833182873756
2	0.3008361401826683
3	0.2900030506976136
4	0.29187082474676096
5	0.31117115658795036
6	0.3300979336193103
7	0.3378180663557861
8	0.32997341534936714
9	0.3435459067731713
10-11	0.33750677068092816
12-13	0.33844065770550186
14-15	0.3324015216132587
16-17	0.2794189977524452
18-19	0.27518537657437786
20-21	0.25532471251844424
22-23	0.2521494966348937
24-25	0.23664697202697066
26-27	0.24106737060995273
28-29	0.22836650707575068
30-31	0.22905135756043807
32-33	0.2251912911922002
34-35	0.23764311818651593
36-37	0.2307323542046707
38-39	0.21186783630828235
40-41	0.236148898947198
42-43	0.22238963011847915
44-45	0.21255268679296968
46-47	0.22058411520430335
48-49	0.21354883295251495
50-51	0.21442046084211705
52-53	0.24928557642620114
54-55	0.23739408164662962
56-57	0.23789215472640224
58-59	0.23235109171393173
60-61	0.24841394853659904
62-63	0.23023428112489805
64-65	0.23807893213131695
66-67	0.23515275278765277
68-69	0.2277439157260349
70-71	0.22780617486100646
72-73	0.2271835835112907
74-75	0.21884085942509915
76-77	0.2254403277320865
78-79	0.23540178932753905
80-81	0.223883849357797
82-83	0.23988444704549275
84-85	0.21529208873171915
86-87	0.2090661752345613
88-89	0.21572790267652023
90-91	0.2060154776209539
92-93	0.22842876621072228
94-95	0.21211687284816866
96-97	0.2026534843324887
98-99	0.1953069064058424
100-101	0.18484737173061716
102-103	0.1956804612156719
104-105	0.19312783668183714
106-107	0.1953069064058424
108-109	0.19343913235669502
110-111	0.1887696972338266
112-113	0.18957906598845714
114-115	0.18434929865084454
116-117	0.19375042803155293
118-119	0.174823651000193
120-121	0.18173441498203824
122-123	0.18098730536237928
124-125	0.17700272072419826
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	803095.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.6417434032182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.23410503995233	65.22898046038239
2	9.320222203351674	14.099957127338087
3	2.1747974105304686	4.935164030439872
4	0.8543344505689912	2.584933891618761
5	0.4143929654812967	1.5672703181517451
6	0.23974499985365713	1.0880837856680952
7	0.16478133038421813	0.8725042977394767
8	0.10287178348698545	0.6225120839963177
9	0.07976388572651433	0.5430131439271754
>10	0.37772249687639514	5.063850186355016
>50	0.024504833189973436	1.3198749849537827
>100	0.012353565658023319	1.5206947429725979
>500	0.0	0.0
>1k	4.050349394486298E-4	0.5531609464567541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2293	0.2855203929796599	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1318	0.16411507978508147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.245182699431574E-4
7	0.0	0.0	0.0	0.0	1.245182699431574E-4
8	0.0	0.0	0.0	0.0	1.245182699431574E-4
9	0.0	0.0	0.0	0.0	1.245182699431574E-4
10-11	0.0	0.0	0.0	0.0	1.245182699431574E-4
12-13	0.0	0.0	0.0	0.0	1.245182699431574E-4
14-15	0.0	0.0	0.0	0.0	1.867774049147361E-4
16-17	0.0	0.0	0.0	0.0	2.490365398863148E-4
18-19	0.0	0.0	0.0	0.0	2.490365398863148E-4
20-21	0.0	0.0	0.0	2.490365398863148E-4	2.490365398863148E-4
22-23	0.0	0.0	0.0	9.961461595452592E-4	2.490365398863148E-4
24-25	0.0	0.0	0.0	0.003548770693379986	2.490365398863148E-4
26-27	0.0	0.0	0.0	0.005914617822299977	2.490365398863148E-4
28-29	0.0	0.0	0.0	0.011704717374656797	2.490365398863148E-4
30-31	0.0	0.0	0.0	0.025277208798460953	2.490365398863148E-4
32-33	0.0	0.0	0.0	0.050741195001836645	2.490365398863148E-4
34-35	0.0	0.0	0.0	0.08162172594773968	2.490365398863148E-4
36-37	0.0	0.0	0.0	0.1262615257223616	2.490365398863148E-4
38-39	0.0	0.0	0.0	0.1879603284791961	2.490365398863148E-4
40-41	0.0	0.0	0.0	0.25544923078838744	2.490365398863148E-4
42-43	6.22591349715787E-5	0.0	0.0	0.3122918210174388	2.490365398863148E-4
44-45	1.245182699431574E-4	0.0	0.0	0.3749867699338185	2.490365398863148E-4
46-47	1.245182699431574E-4	0.0	0.0	0.44963547276474136	2.490365398863148E-4
48-49	1.245182699431574E-4	0.0	0.0	0.5305100890928222	2.490365398863148E-4
50-51	1.245182699431574E-4	0.0	0.0	0.6034155361445408	2.490365398863148E-4
52-53	1.245182699431574E-4	0.0	0.0	0.6716515480733911	2.490365398863148E-4
54-55	1.245182699431574E-4	0.0	0.0	0.7398253008672697	2.490365398863148E-4
56-57	1.245182699431574E-4	0.0	0.0	0.8193924753609474	2.490365398863148E-4
58-59	1.245182699431574E-4	0.0	0.0	0.9043761945971522	2.490365398863148E-4
60-61	1.245182699431574E-4	0.0	0.0	0.9946519403059414	2.490365398863148E-4
62-63	1.245182699431574E-4	0.0	0.0	1.0729116729652157	2.490365398863148E-4
64-65	1.245182699431574E-4	0.0	0.0	1.1552805085326145	2.490365398863148E-4
66-67	1.245182699431574E-4	0.0	0.0	1.2416339287381941	3.112956748578935E-4
68-69	1.245182699431574E-4	0.0	0.0	1.3390694749687149	3.735548098294722E-4
70-71	1.245182699431574E-4	0.0	0.0	1.4421083433466775	3.735548098294722E-4
72-73	1.245182699431574E-4	0.0	0.0	1.5348744544543298	3.735548098294722E-4
74-75	1.245182699431574E-4	0.0	0.0	1.6303799675007316	3.735548098294722E-4
76-77	1.245182699431574E-4	0.0	0.0	1.7340414272284101	3.735548098294722E-4
78-79	1.245182699431574E-4	0.0	0.0	1.8509018235700632	3.735548098294722E-4
80-81	1.245182699431574E-4	0.0	0.0	1.973490060329102	4.3581394480105094E-4
82-83	1.245182699431574E-4	0.0	0.0	2.0953311874684815	6.848504846873657E-4
84-85	1.245182699431574E-4	0.0	0.0	2.220347530491411	7.471096196589444E-4
86-87	1.245182699431574E-4	0.0	0.0	2.3425622124406207	7.471096196589444E-4
88-89	1.245182699431574E-4	0.0	0.0	2.4749251333901965	7.471096196589444E-4
90-91	1.245182699431574E-4	0.0	0.0	2.618370180364714	7.471096196589444E-4
92-93	1.245182699431574E-4	0.0	0.0	2.765613034572498	7.471096196589444E-4
94-95	1.245182699431574E-4	0.0	0.0	2.907128048362896	7.471096196589444E-4
96-97	1.245182699431574E-4	0.0	0.0	3.0643946233011037	7.471096196589444E-4
98-99	1.245182699431574E-4	0.0	0.0	3.2312491050249346	7.471096196589444E-4
100-101	1.245182699431574E-4	0.0	0.0	3.4148512940561204	7.471096196589444E-4
102-103	1.245182699431574E-4	0.0	0.0	3.598889297032107	8.093687546305232E-4
104-105	1.245182699431574E-4	0.0	0.0	3.7848573331922126	8.716278896021019E-4
106-107	1.245182699431574E-4	0.0	0.0	3.9703895554075173	8.716278896021019E-4
108-109	1.245182699431574E-4	0.0	0.0	4.168809418561938	8.716278896021019E-4
110-111	1.245182699431574E-4	0.0	0.0	4.377937852931471	9.338870245736805E-4
112-113	1.245182699431574E-4	0.0	0.0	4.588000174325578	9.961461595452592E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2865	0.0	39.29347	1
GTATCAA	4090	0.0	33.641224	1
TATCAAC	4975	0.0	27.295923	2
ATCAACG	4985	0.0	26.882729	3
CAACGCA	4965	0.0	26.749428	5
TCAACGC	5070	0.0	26.314556	4
AACGCAG	5060	0.0	26.243933	6
ACGCAGA	5545	0.0	23.627775	7
CGCAGAG	5700	0.0	22.774885	8
GCAGAGT	5980	0.0	21.708504	9
CATGGGG	4090	0.0	20.969845	4
AGAGTAC	5665	0.0	20.287663	10-11
GTACATG	7490	0.0	20.278765	1
CAGAGTA	5915	0.0	20.185253	10-11
TACATGG	7690	0.0	19.362867	2
ACATGGG	7425	0.0	19.171558	3
ATGGGGC	1110	0.0	18.779064	5
GAGTACA	6355	0.0	18.459148	12-13
AGTAGCG	165	0.0081128925	18.047411	5
AGTACAT	6395	0.0	17.691885	12-13
>>END_MODULE