##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	803095
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.102573170048377	27.0	14.0	33.0	14.0	33.0
2	28.35688679421488	33.0	27.0	33.0	14.0	33.0
3	31.419749842795685	33.0	33.0	33.0	27.0	33.0
4	31.060451129692005	33.0	33.0	33.0	27.0	33.0
5	32.169270136160726	33.0	33.0	33.0	33.0	33.0
6	35.13704107235134	37.0	37.0	37.0	33.0	37.0
7	35.295282625343205	37.0	37.0	37.0	33.0	37.0
8	35.62857569776926	37.0	37.0	37.0	33.0	37.0
9	35.98126498110435	37.0	37.0	37.0	33.0	37.0
10-11	36.02613825263512	37.0	37.0	37.0	33.0	37.0
12-13	36.11989864212826	37.0	37.0	37.0	37.0	37.0
14-15	36.08999806996682	37.0	37.0	37.0	37.0	37.0
16-17	36.12179443278815	37.0	37.0	37.0	37.0	37.0
18-19	36.12718980942479	37.0	37.0	37.0	37.0	37.0
20-21	36.13927866566222	37.0	37.0	37.0	37.0	37.0
22-23	36.132014269793736	37.0	37.0	37.0	37.0	37.0
24-25	36.12194136434668	37.0	37.0	37.0	37.0	37.0
26-27	36.07623568818135	37.0	37.0	37.0	37.0	37.0
28-29	36.0549885131896	37.0	37.0	37.0	37.0	37.0
30-31	36.02702980344791	37.0	37.0	37.0	37.0	37.0
32-33	35.97359278790181	37.0	37.0	37.0	37.0	37.0
34-35	35.86670568239125	37.0	37.0	37.0	33.0	37.0
36-37	35.78420610264041	37.0	37.0	37.0	33.0	37.0
38-39	35.783496971093086	37.0	37.0	37.0	33.0	37.0
40-41	35.68096489207379	37.0	37.0	37.0	33.0	37.0
42-43	35.64156108555028	37.0	37.0	37.0	33.0	37.0
44-45	35.54734869473724	37.0	37.0	37.0	33.0	37.0
46-47	35.42325440950323	37.0	37.0	37.0	33.0	37.0
48-49	35.27125184442687	37.0	37.0	37.0	33.0	37.0
50-51	35.17509261046327	37.0	37.0	37.0	33.0	37.0
52-53	35.079823682129756	37.0	37.0	37.0	33.0	37.0
54-55	35.01873501889565	37.0	37.0	37.0	33.0	37.0
56-57	35.00783780250157	37.0	37.0	37.0	30.0	37.0
58-59	34.954381486623625	37.0	37.0	37.0	27.0	37.0
60-61	34.92669111375366	37.0	37.0	37.0	27.0	37.0
62-63	34.907628611808065	37.0	37.0	37.0	27.0	37.0
64-65	34.86207671570611	37.0	37.0	37.0	27.0	37.0
66-67	34.85127288801449	37.0	37.0	37.0	27.0	37.0
68-69	34.81184729079374	37.0	37.0	37.0	27.0	37.0
70-71	34.70319078066729	37.0	37.0	37.0	27.0	37.0
72-73	34.7011044770544	37.0	37.0	37.0	27.0	37.0
74-75	34.625948362273455	37.0	37.0	37.0	27.0	37.0
76-77	34.50424794077911	37.0	37.0	37.0	27.0	37.0
78-79	34.50682111082749	37.0	37.0	37.0	27.0	37.0
80-81	34.50530945903037	37.0	37.0	37.0	27.0	37.0
82-83	34.42490552176268	37.0	37.0	37.0	27.0	37.0
84-85	34.34281560711996	37.0	37.0	37.0	27.0	37.0
86-87	34.21758571526407	37.0	37.0	37.0	27.0	37.0
88-89	34.13103555619198	37.0	37.0	37.0	27.0	37.0
90-91	34.07935238047803	37.0	37.0	37.0	27.0	37.0
92-93	33.910622653608854	37.0	37.0	37.0	24.5	37.0
94-95	33.95234623550141	37.0	37.0	37.0	27.0	37.0
96-97	33.94444741904756	37.0	37.0	37.0	22.0	37.0
98-99	33.9151090468749	37.0	37.0	37.0	22.0	37.0
100-101	33.78539961025781	37.0	35.0	37.0	22.0	37.0
102-103	33.712616813701985	37.0	33.0	37.0	22.0	37.0
104-105	33.56520399205573	37.0	33.0	37.0	22.0	37.0
106-107	33.52527969916386	37.0	33.0	37.0	22.0	37.0
108-109	33.4005360511521	37.0	33.0	37.0	22.0	37.0
110-111	33.32686294896619	37.0	33.0	37.0	22.0	37.0
112-113	33.18886619889303	37.0	33.0	37.0	14.0	37.0
114-115	33.15719435434164	37.0	33.0	37.0	14.0	37.0
116-117	32.99599860539537	37.0	33.0	37.0	14.0	37.0
118-119	32.87819685093295	37.0	33.0	37.0	14.0	37.0
120-121	32.784747134523315	37.0	33.0	37.0	14.0	37.0
122-123	32.714231815663155	37.0	33.0	37.0	14.0	37.0
124-125	31.291119979579	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	14.0
17	23.0
18	134.0
19	443.0
20	1654.0
21	3906.0
22	6469.0
23	9026.0
24	8250.0
25	6603.0
26	6548.0
27	7743.0
28	9853.0
29	12762.0
30	16894.0
31	23305.0
32	32797.0
33	49033.0
34	80627.0
35	166657.0
36	360341.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.08174652995746	15.26619436223956	12.909903446626611	27.742155661176376
2	18.094870469869694	20.476157864262635	37.61422994788911	23.81474171797857
3	20.42396191714264	25.731206052614215	29.041914303609428	24.80291772663372
4	14.960041639376106	18.68554550820211	33.38334873226529	32.9710641201565
5	16.594712455031214	34.658934546364705	32.115154964093456	16.63119803451063
6	29.87585296608059	33.97133535886835	18.74308910693829	17.409722568112766
7	26.2317658558452	31.97330328292419	22.34094347493136	19.453987386299254
8	26.37023017202199	31.57198089889739	20.88295905216693	21.17482987691369
9	26.771801592588673	16.11976167203133	20.80164862189405	36.30678811348595
10-11	25.839533305524252	25.025930929715663	26.174798747346205	22.959737017413882
12-13	27.050428062949006	24.2418544766525	26.275799423355533	22.431918037042962
14-15	24.957134585572067	24.337469415199944	25.3496161724329	25.35577982679509
16-17	23.60536424706915	26.400114556808347	26.315691169786888	23.678830026335614
18-19	23.461732422689717	24.829627877150276	28.80039098736762	22.908248712792386
20-21	25.01514463829996	23.622211472071296	28.763294751083162	22.59934913854558
22-23	25.039378902869526	23.56109800210436	28.46437843592601	22.935144659100107
24-25	23.995106431991235	24.255909948387178	28.61386261899277	23.135121000628818
26-27	23.425995679216033	24.48626874778202	28.840859425099147	23.2468761479028
28-29	23.275981757903093	24.349339268759632	29.117499649789096	23.25717932354818
30-31	23.676288845929953	24.08128577965801	29.002698941902704	23.23972643250933
32-33	23.53630793100142	24.293695966449548	28.608099475898683	23.56189662665035
34-35	23.711578331330667	24.35857526195531	28.73402274948792	23.195823657226107
36-37	23.635472758640226	24.44659032399253	28.520606980915574	23.397329936451666
38-39	23.542420261612882	24.4129274867855	28.64860321630691	23.396049035294702
40-41	23.451787774435783	24.357488891199438	28.73221144357439	23.45851189079039
42-43	23.578758359970266	24.37537589514409	28.5507849126429	23.495080832242746
44-45	23.63917095735872	24.5866304733562	28.16267066785374	23.611527901431337
46-47	23.70298657070459	24.48197286746898	28.0337319993276	23.781308562498833
48-49	23.81916211656155	24.77066847633219	27.912388945267995	23.497780461838264
50-51	23.608601722087673	25.057122756336426	27.71228808546934	23.62198743610656
52-53	23.63014338278784	24.860010335016405	27.582415529918624	23.92743075227713
54-55	23.561222520374304	24.870158574016774	27.494567890473732	24.074051015135193
56-57	23.728886370852763	24.809455917419484	27.22598198220634	24.235675729521414
58-59	23.51060584364241	24.948169270136162	27.460512143644277	24.080712742577155
60-61	23.651871820892918	24.83255405649394	27.76315379874112	23.75242032387202
62-63	23.410181858933253	24.843885219058766	27.727728350942293	24.01820457106569
64-65	23.359191627391528	24.58918309789004	27.932498645863813	24.119126628854616
66-67	23.646455276150395	24.587937915190604	27.740865028421297	24.024741780237704
68-69	23.434649698977083	24.65822847857352	27.788991339754325	24.118130482695076
70-71	23.37899003231249	24.64054688424159	27.942148811784406	24.03831427166151
72-73	23.496348501733916	24.705358643747	28.0105093419832	23.787783512535874
74-75	23.525596533879288	24.646515748693787	28.093882825282147	23.73400489214478
76-77	23.4488079354228	24.466909668320813	28.03222877469459	24.052053621561793
78-79	23.299572153626237	24.652405950999388	28.021322588196618	24.026699307177758
80-81	23.13456066841407	24.89126442077214	28.050541965769927	23.923632945043863
82-83	23.313804717997247	24.722355387594245	28.239809736083526	23.72403015832498
84-85	23.319505487316306	24.765572526408526	27.9642536265385	23.950668359736667
86-87	23.101949639762847	24.92156352839709	27.961612121280154	24.014874710559912
88-89	23.36107811626607	24.534855865208016	27.918629721312783	24.18543629721313
90-91	23.40860004661069	24.589632735162784	27.865280143969713	24.136487074256816
92-93	23.379840186334977	24.837078466053157	27.781355654295247	24.001725693316615
94-95	23.04346580898055	24.831432552475373	27.84684425550778	24.278257383036294
96-97	23.068877281019056	24.739227613171543	27.785442569060947	24.406452536748453
98-99	23.036378012563894	24.848181099371804	27.580610015004453	24.53483087305985
100-101	23.218548241490733	25.03651498266083	27.333067694357453	24.41186908149098
102-103	23.23929500202031	24.900743312275207	27.53449313872776	24.325468546976726
104-105	23.24329675337855	25.043862325219994	27.284918955871202	24.427921965530253
106-107	23.25847082753026	24.98441964177317	27.3126014435412	24.444508087155373
108-109	23.215335229338606	25.059519955372483	27.03345320336898	24.69169161191993
110-111	23.017901380223623	25.502540485584202	26.983188155316718	24.496369978875464
112-113	23.020066119201342	25.436467665718254	26.9842297611117	24.55923645396871
114-115	23.03727832607391	25.352387142725508	26.93713313759037	24.67320139361021
116-117	23.14438374825206	25.334053884438713	26.85555399214782	24.666008375161407
118-119	22.948712042067356	25.197953412618464	26.996250745555	24.85708379975918
120-121	23.014400537918924	25.157235445370723	27.24017706497986	24.588186951730492
122-123	22.891625523754975	25.366924211955	27.204129025831314	24.537321238458713
124-125	22.920359436899624	25.253225200927172	27.021957079503593	24.804458282669614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	397.0
1	371.0
2	622.5
3	819.5
4	570.0
5	486.5
6	736.5
7	1534.5
8	2476.5
9	2913.0
10	2574.5
11	1872.5
12	1610.0
13	1533.0
14	1468.5
15	1441.5
16	1393.5
17	1339.0
18	1286.0
19	1205.5
20	1154.0
21	1158.0
22	1204.5
23	1253.5
24	1332.0
25	1509.0
26	1723.5
27	2056.5
28	2561.0
29	3307.0
30	4484.0
31	5663.0
32	6881.5
33	8326.5
34	9676.5
35	11040.0
36	12724.5
37	14607.5
38	16038.5
39	16976.0
40	17515.0
41	17962.5
42	19013.0
43	20822.5
44	22998.0
45	25359.5
46	27909.0
47	30340.0
48	32417.5
49	33914.5
50	34282.5
51	33754.0
52	33199.0
53	33028.0
54	32666.5
55	31738.0
56	30379.5
57	27772.0
58	25085.0
59	22706.0
60	19911.5
61	16634.5
62	13439.5
63	10649.0
64	7996.5
65	6195.0
66	4905.0
67	3760.0
68	2970.5
69	2461.5
70	2110.0
71	1789.5
72	1463.0
73	1308.0
74	1082.5
75	889.0
76	713.5
77	487.0
78	364.5
79	270.5
80	205.5
81	169.0
82	134.5
83	87.5
84	53.0
85	32.5
86	14.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0277675741973241
2	0.0
3	4.980730797726296E-4
4	0.0011206644294884168
5	0.004731694257839982
6	0.001867774049147361
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.22591349715787E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.848504846873657E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	9.338870245736805E-4
30-31	3.112956748578935E-4
32-33	7.471096196589445E-4
34-35	0.0
36-37	6.848504846873657E-4
38-39	0.0
40-41	0.00193003318411894
42-43	0.001494219239317889
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07271866964680393
76-77	0.09569229045131647
78-79	3.7355480982947226E-4
80-81	0.0
82-83	0.0
84-85	0.07570710812543971
86-87	0.1657960764293141
88-89	0.20209315211774445
90-91	0.08766086203998281
92-93	0.13678331953255843
94-95	4.980730797726296E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	6.22591349715787E-5
104-105	0.001743255779204204
106-107	6.22591349715787E-5
108-109	3.7355480982947226E-4
110-111	6.22591349715787E-5
112-113	0.0
114-115	1.245182699431574E-4
116-117	7.471096196589445E-4
118-119	2.490365398863148E-4
120-121	0.0
122-123	0.0
124-125	6.848504846873657E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	803095.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.75184544562418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.40192303757307	62.13147505588608
2	9.90018303111829	14.405131715266183
3	2.3064894894678254	5.034041005791597
4	0.8495733804582798	2.47232125079269
5	0.43778104578735116	1.592468949107255
6	0.2619479574455254	1.1434318388924285
7	0.18238163708207478	0.9288020471180517
8	0.11578040150429098	0.6738590300697993
9	0.08451658341260761	0.553386367262361
>10	0.4106131145632288	5.357306244339872
>50	0.0317787735974852	1.5866381220360368
>100	0.01557763535713533	1.8833963513956042
>500	6.231054141057006E-4	0.3256353607603945
>1k	8.308072188076006E-4	1.9121066612816853
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4632	0.5767686263767051	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4289	0.5340588597862022	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2482	0.3090543459989167	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1251	0.15577235569888992	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	914	0.11380969872804586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	1.245182699431574E-4
20-21	0.0	0.0	0.0	2.490365398863148E-4	1.245182699431574E-4
22-23	0.0	0.0	0.0	9.961461595452592E-4	1.245182699431574E-4
24-25	0.0	0.0	0.0	0.003548770693379986	1.245182699431574E-4
26-27	0.0	0.0	0.0	0.005976876957271556	1.245182699431574E-4
28-29	0.0	0.0	0.0	0.01158019910471364	1.245182699431574E-4
30-31	0.0	0.0	0.0	0.025277208798460953	1.245182699431574E-4
32-33	0.0	0.0	0.0	0.05142604548652401	1.245182699431574E-4
34-35	0.0	0.0	0.0	0.08280464951219968	1.245182699431574E-4
36-37	0.0	0.0	0.0	0.12750670842179318	1.245182699431574E-4
38-39	0.0	0.0	0.0	0.18901873377371295	2.490365398863148E-4
40-41	0.0	0.0	0.0	0.25632085867798954	2.490365398863148E-4
42-43	0.0	0.0	0.0	0.31291441236715456	2.490365398863148E-4
44-45	0.0	0.0	0.0	0.37523580647370486	2.490365398863148E-4
46-47	0.0	0.0	0.0	0.4496354727647414	3.735548098294722E-4
48-49	0.0	0.0	0.0	0.5314439761173959	3.735548098294722E-4
50-51	0.0	0.0	0.0	0.6044739414390576	3.735548098294722E-4
52-53	1.245182699431574E-4	0.0	0.0	0.6732702855826521	3.735548098294722E-4
54-55	1.245182699431574E-4	1.245182699431574E-4	0.0	0.7423156662661329	3.735548098294722E-4
56-57	1.245182699431574E-4	1.245182699431574E-4	0.0	0.8238128739439294	3.735548098294722E-4
58-59	1.245182699431574E-4	1.245182699431574E-4	0.0	0.9096682210697364	3.735548098294722E-4
60-61	1.245182699431574E-4	1.245182699431574E-4	0.0	0.9993836345637814	3.735548098294722E-4
62-63	1.245182699431574E-4	1.245182699431574E-4	0.0	1.0773943306831695	3.735548098294722E-4
64-65	1.245182699431574E-4	1.245182699431574E-4	0.0	1.1595763888456534	3.735548098294722E-4
66-67	1.245182699431574E-4	1.245182699431574E-4	0.0	1.2462411047260908	3.735548098294722E-4
68-69	1.245182699431574E-4	1.245182699431574E-4	0.0	1.3428672822019811	3.735548098294722E-4
70-71	1.245182699431574E-4	1.245182699431574E-4	0.0	1.4453458183651997	3.735548098294722E-4
72-73	1.245182699431574E-4	1.245182699431574E-4	0.0	1.5385477434176529	3.735548098294722E-4
74-75	1.245182699431574E-4	1.245182699431574E-4	0.0	1.634738106948742	3.735548098294722E-4
76-77	1.245182699431574E-4	1.245182699431574E-4	0.0	1.7372166431119607	3.735548098294722E-4
78-79	1.245182699431574E-4	1.245182699431574E-4	0.0	1.8534544481038981	3.735548098294722E-4
80-81	1.245182699431574E-4	1.245182699431574E-4	0.0	1.9747352430285332	3.735548098294722E-4
82-83	1.245182699431574E-4	1.245182699431574E-4	0.0	2.095517964873396	3.735548098294722E-4
84-85	1.245182699431574E-4	1.245182699431574E-4	0.0	2.220409789626383	3.735548098294722E-4
86-87	1.245182699431574E-4	1.245182699431574E-4	0.0	2.3434338403302224	3.735548098294722E-4
88-89	1.245182699431574E-4	1.245182699431574E-4	0.0	2.476045797819685	3.735548098294722E-4
90-91	1.245182699431574E-4	1.245182699431574E-4	0.0	2.6181211438248275	3.735548098294722E-4
92-93	1.245182699431574E-4	1.245182699431574E-4	0.0	2.7641188153331795	3.735548098294722E-4
94-95	1.245182699431574E-4	1.245182699431574E-4	0.0	2.9058206065284926	4.980730797726296E-4
96-97	1.245182699431574E-4	1.245182699431574E-4	0.0	3.0628381449268147	4.980730797726296E-4
98-99	1.245182699431574E-4	1.245182699431574E-4	0.0	3.229007776165958	4.980730797726296E-4
100-101	1.245182699431574E-4	1.245182699431574E-4	0.0	3.412423187792229	4.980730797726296E-4
102-103	1.245182699431574E-4	1.245182699431574E-4	0.0	3.5945311575840964	4.980730797726296E-4
104-105	1.245182699431574E-4	1.245182699431574E-4	0.0	3.7786936788300265	4.980730797726296E-4
106-107	1.245182699431574E-4	1.245182699431574E-4	0.0	3.9635410505606434	4.980730797726296E-4
108-109	1.245182699431574E-4	1.245182699431574E-4	0.0	4.161587358905235	4.980730797726296E-4
110-111	1.245182699431574E-4	1.245182699431574E-4	0.0	4.371587421164371	4.980730797726296E-4
112-113	1.245182699431574E-4	1.245182699431574E-4	0.0	4.582023297368306	4.980730797726296E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3185	0.0	65.188385	1
GTATCAA	4795	0.0	43.165493	2
TATCAAC	5940	0.0	35.245388	3
ATCAACG	6115	0.0	33.944935	4
CAACGCA	6260	0.0	33.158676	6
TCAACGC	6400	0.0	32.433327	5
AACGCAG	6640	0.0	31.26104	7
TATCACG	80	0.0053715757	29.738295	2
ACGCAGA	7260	0.0	28.673286	8
CGCAGAG	7590	0.0	27.191536	9
GAGTACT	3545	0.0	26.592495	12-13
CAGAGTA	7870	0.0	25.355015	10-11
CTCAACG	440	0.0	24.337393	1
GTGGTAT	1355	0.0	24.147804	1
GCAGAGT	7970	0.0	24.104063	9
GTACTTT	4130	0.0	22.826473	14-15
CTATTAC	235	2.910383E-7	22.783941	1
GTACATG	6300	0.0	22.09681	1
ACATGGG	6270	0.0	22.007288	3
TACATGG	6510	0.0	21.652767	2
>>END_MODULE