FastQCFastQC Report
Sun 1 Jan 2017
ERR1142630_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142630_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662245
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18860.28478886212806437No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11010.16625267083934192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.042.2922481
GTATCAA29350.038.5609661
CAACGCA36300.030.507835
ATCAACG36150.030.4743333
TATCAAC37600.030.0909962
AACGCAG36600.029.9301436
TCAACGC37050.029.8947934
ACGCAGA40450.027.2306617
CGCAGAG42350.026.1495678
GCAGAGT44100.025.1118859
CAGAGTA43800.022.29247510-11
AGAGTAC42300.022.02736510-11
TCTAACG1703.909617E-421.0171172
GAGTACA46550.020.84682812-13
CTAACGC2004.8743706E-520.8419743
GTACATG55800.020.8162671
TACATGG56350.020.2898162
ATGGGGG17850.019.83897618-19
CCCGTAA750.0047191519.823963104-105
CATGGGG32000.019.725444
Produced by FastQC (version 0.11.5)