##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142630_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	662245
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27201564375722	33.0	33.0	33.0	27.0	33.0
2	31.352683674470928	33.0	33.0	33.0	27.0	33.0
3	31.461806431154635	33.0	33.0	33.0	27.0	33.0
4	31.392064870251946	33.0	33.0	33.0	27.0	33.0
5	31.420033371335382	33.0	33.0	33.0	27.0	33.0
6	34.639858360576525	37.0	37.0	37.0	27.0	37.0
7	34.750293320447874	37.0	37.0	37.0	27.0	37.0
8	34.76495707781863	37.0	37.0	37.0	27.0	37.0
9	34.68488701311448	37.0	37.0	37.0	27.0	37.0
10-11	34.71966417262493	37.0	37.0	37.0	27.0	37.0
12-13	34.73234981011559	37.0	37.0	37.0	27.0	37.0
14-15	34.70084560849837	37.0	37.0	37.0	27.0	37.0
16-17	34.69528120257608	37.0	37.0	37.0	27.0	37.0
18-19	34.70067195675317	37.0	37.0	37.0	27.0	37.0
20-21	34.76512242448037	37.0	37.0	37.0	27.0	37.0
22-23	34.83665788341173	37.0	37.0	37.0	27.0	37.0
24-25	34.87538146758375	37.0	37.0	37.0	27.0	37.0
26-27	34.85900006795068	37.0	37.0	37.0	27.0	37.0
28-29	34.902147241579776	37.0	37.0	37.0	27.0	37.0
30-31	34.95618992970879	37.0	37.0	37.0	27.0	37.0
32-33	34.970168895197396	37.0	37.0	37.0	27.0	37.0
34-35	34.972288201496426	37.0	37.0	37.0	27.0	37.0
36-37	34.91413147702134	37.0	37.0	37.0	27.0	37.0
38-39	34.90630808839629	37.0	37.0	37.0	27.0	37.0
40-41	34.880769956738064	37.0	37.0	37.0	27.0	37.0
42-43	34.87369553564014	37.0	37.0	37.0	27.0	37.0
44-45	34.89721175697815	37.0	37.0	37.0	27.0	37.0
46-47	34.89783841327605	37.0	37.0	37.0	27.0	37.0
48-49	34.886517829504186	37.0	37.0	37.0	27.0	37.0
50-51	34.86341610733188	37.0	37.0	37.0	27.0	37.0
52-53	34.840335525372026	37.0	37.0	37.0	27.0	37.0
54-55	34.80797816518056	37.0	37.0	37.0	27.0	37.0
56-57	34.742592243052044	37.0	37.0	37.0	27.0	37.0
58-59	34.6948712334559	37.0	37.0	37.0	27.0	37.0
60-61	34.66362750945647	37.0	37.0	37.0	27.0	37.0
62-63	34.710931754864134	37.0	37.0	37.0	27.0	37.0
64-65	34.64623062461778	37.0	37.0	37.0	27.0	37.0
66-67	34.61902468119805	37.0	37.0	37.0	27.0	37.0
68-69	34.58685305287318	37.0	37.0	37.0	27.0	37.0
70-71	34.54633632568007	37.0	37.0	37.0	27.0	37.0
72-73	34.5011785668446	37.0	37.0	37.0	27.0	37.0
74-75	34.42041842520517	37.0	37.0	37.0	27.0	37.0
76-77	34.36981253161595	37.0	37.0	37.0	27.0	37.0
78-79	34.30298454499467	37.0	37.0	37.0	27.0	37.0
80-81	34.231825834849644	37.0	37.0	37.0	27.0	37.0
82-83	34.19302448489607	37.0	37.0	37.0	27.0	37.0
84-85	34.16057501377889	37.0	37.0	37.0	27.0	37.0
86-87	34.093523544911626	37.0	37.0	37.0	27.0	37.0
88-89	34.03524903925285	37.0	37.0	37.0	27.0	37.0
90-91	33.96567584504224	37.0	37.0	37.0	27.0	37.0
92-93	33.834291689631485	37.0	35.0	37.0	22.0	37.0
94-95	33.77121458070654	37.0	33.0	37.0	22.0	37.0
96-97	33.671601899599096	37.0	33.0	37.0	22.0	37.0
98-99	33.62518554311471	37.0	33.0	37.0	22.0	37.0
100-101	33.512107301678384	37.0	33.0	37.0	22.0	37.0
102-103	33.439202258982704	37.0	33.0	37.0	22.0	37.0
104-105	33.312869859342086	37.0	33.0	37.0	22.0	37.0
106-107	33.23086546519792	37.0	33.0	37.0	18.0	37.0
108-109	33.09959531593293	37.0	33.0	37.0	14.0	37.0
110-111	33.020692492959554	37.0	33.0	37.0	14.0	37.0
112-113	32.93813014820799	37.0	33.0	37.0	14.0	37.0
114-115	32.76139948206479	37.0	33.0	37.0	14.0	37.0
116-117	32.56084379648016	37.0	33.0	37.0	14.0	37.0
118-119	32.45060513858164	37.0	33.0	37.0	14.0	37.0
120-121	32.37093522789904	37.0	33.0	37.0	14.0	37.0
122-123	32.165147339730765	37.0	33.0	37.0	14.0	37.0
124-125	30.49712115606762	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1107.0
1	95.0
2	47.0
3	25.0
4	26.0
5	27.0
6	21.0
7	16.0
8	16.0
9	21.0
10	18.0
11	22.0
12	18.0
13	13.0
14	61.0
15	220.0
16	318.0
17	597.0
18	1370.0
19	2468.0
20	3595.0
21	4324.0
22	5042.0
23	5600.0
24	6404.0
25	7243.0
26	8689.0
27	10399.0
28	12730.0
29	15173.0
30	18403.0
31	22738.0
32	28619.0
33	38512.0
34	58678.0
35	110123.0
36	299467.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.43477812865568	19.66732936120691	12.529169672026816	24.368722838110592
2	17.637437280681162	22.55108794259431	35.82675913206913	23.984715644655395
3	20.031588973842936	26.623780773127265	28.152953629627607	25.19167662340219
4	14.963896074000932	19.332864870758847	33.24817208730366	32.45506696793656
5	15.87852882282197	36.929988518735065	30.79357049163443	16.397912166808542
6	29.878550360622263	35.273719946251305	18.166393731944268	16.681335961182167
7	26.686249884485274	32.80481874413905	21.215017974848845	19.293913396526833
8	25.8286813661256	33.60081197973081	20.122554403399405	20.44795225074419
9	26.361920172599785	18.430390410045696	19.692617238161038	35.51507217919349
10-11	25.491350146727083	26.961953977538712	25.28743207149556	22.259263804238646
12-13	26.22058332266517	25.80516874512831	24.930025671772487	23.044222260434033
14-15	24.667801326901067	26.12649870968377	23.150780287209923	26.054919676205234
16-17	24.29051624283627	27.97151920296164	23.84248499118019	23.8954795630219
18-19	24.293277190687405	27.11184090494435	25.289551759719163	23.305330144649076
20-21	26.18520307062095	25.706762057152545	24.748593288050103	23.359441584176402
22-23	26.164931521364803	25.566921356478694	24.43833895316054	23.82980816899596
24-25	24.951698114921026	26.247013532095686	24.686293162360172	24.114995190623116
26-27	24.40675708504782	26.47783801129006	24.76540842281958	24.349996480842538
28-29	24.296728027811536	26.269991055388488	25.12293165317912	24.310349263620857
30-31	24.66639424277509	26.017011358562815	25.083278470188503	24.233315928473594
32-33	24.478018984429863	26.187132237208516	24.795157638569385	24.539691139792236
34-35	24.67034563760421	26.306281898373218	24.875055245106644	24.148317218915924
36-37	24.649479668004577	26.367725492332745	24.643879818141095	24.338915021521583
38-39	24.47430383845965	26.36251244631816	24.7584884646464	24.404695250575784
40-41	24.36241092672531	26.251339504640587	24.99190242957384	24.394347139060258
42-43	24.43523483864231	26.25216882685479	24.924426835718506	24.3881694987844
44-45	24.492101286931465	26.341805184497503	24.813134282283563	24.352959246287465
46-47	24.51219632154372	26.230887561696658	24.710367157061928	24.5465489596977
48-49	24.524066234635857	26.426212391188386	24.8145331835223	24.235188190653457
50-51	24.2385700385425	26.689355340035014	24.771158650725074	24.30091597069741
52-53	24.25054570445071	26.354800231299976	24.789665371911585	24.604988692337734
54-55	24.206744722668102	26.222699508611967	24.952794812007937	24.61776095671199
56-57	24.363652875424748	26.182447005759172	24.651687263976026	24.802212854840054
58-59	24.354948929436578	26.24195270647121	24.871216816620468	24.531881547471748
60-61	24.382717684590556	26.275113813969508	25.04028427948094	24.301884221959
62-63	24.049694160096198	26.354144673663566	24.93471152369039	24.661449642549847
64-65	24.159058899743975	26.08905911921698	25.081072572155534	24.67080940888351
66-67	24.52661698677078	25.996353847795472	24.808516467091927	24.66851269834183
68-69	24.381154028930936	25.98108804290907	24.761027686887246	24.876730241272742
70-71	24.259735306514873	26.009929978591746	24.926425684454916	24.803909030438465
72-73	24.519717918700714	26.12801115127703	24.927069467703596	24.425201462318658
74-75	24.431312164467936	26.10837662067426	25.07689572070112	24.383415494156683
76-77	24.418462106582236	25.80212123230036	25.03223619292075	24.74718046819665
78-79	24.35829131962155	25.963263038881667	25.02304384612008	24.6554017953767
80-81	24.25683349967954	26.052842855424085	25.13701807208403	24.553305572812345
82-83	24.426371690256605	25.958241947861467	25.183035829159493	24.43235053272243
84-85	24.29249173356739	26.149166546863295	24.92702083065352	24.63132088891579
86-87	24.071032277150266	26.253812299076422	25.10121365595604	24.573941767817274
88-89	24.34098673234289	25.753630919278013	25.124561622535722	24.78082072584337
90-91	24.585884905737462	25.805948852038306	25.019255008950363	24.588911233273866
92-93	24.330390418614297	26.080323637379028	24.993132694503657	24.59615324950302
94-95	23.899256865032708	26.063291311630948	25.31675163312648	24.720700190209865
96-97	24.057460951035026	26.043764497254507	25.13508074583031	24.763693805880155
98-99	23.82304725059329	26.21859839151745	25.0893622429488	24.868992114940458
100-101	24.062372358289334	26.40182745109904	24.885633027245362	24.650167163366262
102-103	24.170664157092485	26.306727358418485	25.10706415376387	24.41554433072516
104-105	24.109899919057742	26.514565824211566	25.032187786040105	24.34334647069058
106-107	24.056959530864553	26.439477094319553	25.06390321358084	24.439660161235054
108-109	24.0079914427111	26.42085642485063	25.029994386926834	24.541157745511434
110-111	23.812423504778447	26.893790970802844	24.833620529237248	24.46016499518146
112-113	23.83263500843058	26.933984279557905	24.81533283962683	24.418047872384683
114-115	23.778435350376768	26.88100306043566	24.880903214289496	24.459658374898076
116-117	23.92467017671266	26.923792665214236	24.796886347131444	24.35465081094166
118-119	23.73606276915816	26.60549487285373	25.028551375079605	24.629890982908503
120-121	23.88261891993492	26.60725050620723	25.072328825561403	24.437801748296444
122-123	23.800774989164825	26.811327276729223	25.08095180105939	24.306945933046563
124-125	23.855073406451115	26.73339590775391	24.798227747192282	24.613302938602693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2087.0
1	1351.5
2	517.0
3	357.0
4	282.0
5	241.5
6	187.5
7	171.0
8	182.0
9	170.5
10	159.0
11	164.0
12	155.5
13	142.0
14	145.5
15	172.5
16	188.5
17	207.5
18	249.5
19	282.5
20	316.0
21	388.0
22	483.0
23	598.0
24	768.5
25	966.5
26	1219.0
27	1596.5
28	2081.0
29	2785.5
30	3800.0
31	4842.0
32	5955.5
33	6980.0
34	8014.5
35	9245.0
36	10479.5
37	11898.5
38	13119.5
39	13906.5
40	14485.0
41	14990.5
42	15927.0
43	17552.5
44	19636.5
45	21716.5
46	23848.5
47	25882.5
48	27653.5
49	28732.0
50	28669.5
51	28165.0
52	28022.0
53	28246.0
54	27747.0
55	26920.5
56	25892.0
57	23616.0
58	21144.0
59	19042.5
60	16727.5
61	14182.0
62	11657.5
63	9286.5
64	7115.0
65	5488.5
66	4322.5
67	3406.5
68	2825.5
69	2408.5
70	2062.0
71	1765.0
72	1480.0
73	1316.0
74	1086.5
75	924.0
76	766.5
77	528.0
78	364.5
79	251.0
80	187.0
81	139.0
82	102.5
83	66.5
84	41.0
85	20.5
86	8.0
87	7.0
88	3.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.0
95	1.0
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34926651012842674
2	0.2962649774630235
3	0.28509086516319493
4	0.29128192738337016
5	0.30789209431554787
6	0.3225392415193773
7	0.3255592718706823
8	0.3216332324139857
9	0.33537437051242364
10-11	0.32857930222198733
12-13	0.3294098105685962
14-15	0.32246374076059464
16-17	0.2718027316174528
18-19	0.26946220809519134
20-21	0.25096452219344806
22-23	0.24726498501309938
24-25	0.23503386209031402
26-27	0.23790289092405378
28-29	0.2283142945586603
30-31	0.2280122915235298
32-33	0.22544526572492052
34-35	0.23450535677883563
36-37	0.22846529607622557
38-39	0.2124591352143089
40-41	0.23450535677883563
42-43	0.22068871792161512
44-45	0.21283663900822206
46-47	0.2191787027459626
48-49	0.2132896435609178
50-51	0.21381814887239617
52-53	0.24628347514892523
54-55	0.23639287574840126
56-57	0.23556236740179237
58-59	0.23276883932683523
60-61	0.24575496983744688
62-63	0.23005081201066072
64-65	0.23729888485379277
66-67	0.23382584994979197
68-69	0.22635127483031206
70-71	0.22929580442283443
72-73	0.22854079683500822
74-75	0.21857469667570156
76-77	0.22604927179518153
78-79	0.23375034919100937
80-81	0.22265173764996338
82-83	0.23888440078822792
84-85	0.2174421852939622
86-87	0.20996761017448226
88-89	0.21555466632439657
90-91	0.2080800912049166
92-93	0.22733278469448617
94-95	0.21079811852109115
96-97	0.20294603960769805
98-99	0.1980384902868274
100-101	0.1834668438417806
102-103	0.1977364872516969
104-105	0.19328194248352196
106-107	0.1939614493125656
108-109	0.19448995462404398
110-111	0.18965790606195593
112-113	0.19003540985586903
114-115	0.1854298635701289
116-117	0.19403695007134822
118-119	0.17455775430543077
120-121	0.1814283233546497
122-123	0.1817303263897802
124-125	0.17599226872230064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	662245.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.48393344727756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.54126953896872	66.19016699861393
2	9.096581983321345	13.91484742020108
3	2.129369215789898	4.885876001554687
4	0.8085762684208042	2.4737237400377934
5	0.43711833363149627	1.6716264769028113
6	0.24744021895361704	1.135512074317694
7	0.15960797284198558	0.8545211900750918
8	0.11097483744462468	0.6790233665149698
9	0.07187054109770777	0.49472475139232175
>10	0.3639634515702103	4.961505054911881
>50	0.02438288153631472	1.2425340677239256
>100	0.008366661481484546	0.9498169599169728
>500	0.0	0.0
>1k	4.780949417967666E-4	0.546121897836818
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1886	0.28478886212806437	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1101	0.16625267083934192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5100151756525153E-4	0.0
3	0.0	0.0	0.0	1.5100151756525153E-4	0.0
4	0.0	0.0	0.0	3.0200303513050307E-4	0.0
5	0.0	0.0	0.0	3.0200303513050307E-4	0.0
6	0.0	0.0	0.0	3.0200303513050307E-4	0.0
7	0.0	0.0	0.0	3.0200303513050307E-4	0.0
8	0.0	0.0	0.0	3.0200303513050307E-4	0.0
9	0.0	0.0	0.0	3.0200303513050307E-4	0.0
10-11	0.0	0.0	0.0	3.0200303513050307E-4	0.0
12-13	0.0	0.0	0.0	3.0200303513050307E-4	0.0
14-15	0.0	0.0	0.0	3.775037939131288E-4	1.5100151756525153E-4
16-17	0.0	0.0	0.0	5.285053114783804E-4	1.5100151756525153E-4
18-19	0.0	0.0	0.0	6.040060702610061E-4	1.5100151756525153E-4
20-21	0.0	0.0	0.0	8.305083466088835E-4	1.5100151756525153E-4
22-23	0.0	0.0	0.0	0.002265022763478773	1.5100151756525153E-4
24-25	0.0	0.0	0.0	0.005964559943827436	1.5100151756525153E-4
26-27	0.0	0.0	0.0	0.009362094089045595	2.2650227634787731E-4
28-29	0.0	0.0	0.0	0.015477655550438282	3.0200303513050307E-4
30-31	0.0	0.0	0.0	0.028161783025919408	3.0200303513050307E-4
32-33	0.0	0.0	0.0	0.05058550838435927	3.0200303513050307E-4
34-35	0.0	0.0	0.0	0.07723727623462616	3.775037939131288E-4
36-37	0.0	0.0	0.0	0.12065021253463598	4.530045526957546E-4
38-39	0.0	0.0	0.0	0.1855808650876941	4.530045526957546E-4
40-41	0.0	0.0	0.0	0.2511155237110133	4.530045526957546E-4
42-43	0.0	0.0	0.0	0.3047210624466776	4.530045526957546E-4
44-45	0.0	0.0	0.0	0.36059162394582067	5.285053114783804E-4
46-47	0.0	0.0	0.0	0.43012782278461903	6.040060702610061E-4
48-49	7.550075878262577E-5	0.0	0.0	0.5077426028131583	7.550075878262576E-4
50-51	1.5100151756525153E-4	0.0	0.0	0.5766747955816955	7.550075878262576E-4
52-53	1.5100151756525153E-4	0.0	0.0	0.6337533692213606	9.81509864174135E-4
54-55	1.5100151756525153E-4	0.0	0.0	0.6941539762474613	0.0010570106229567608
56-57	1.5100151756525153E-4	0.0	0.0	0.7633126712923466	0.0010570106229567608
58-59	3.0200303513050307E-4	0.0	0.0	0.8464390067120174	0.0010570106229567608
60-61	3.0200303513050307E-4	0.0	0.0	0.9268473148155139	0.0010570106229567608
62-63	3.0200303513050307E-4	0.0	0.0	1.001895069045444	0.0010570106229567608
64-65	3.0200303513050307E-4	0.0	0.0	1.0752063058233734	0.0010570106229567608
66-67	3.0200303513050307E-4	0.0	0.0	1.1631646898051327	0.0010570106229567608
68-69	3.0200303513050307E-4	0.0	0.0	1.2533125957915878	0.0010570106229567608
70-71	7.550075878262576E-4	0.0	0.0	1.3490475579279573	0.0010570106229567608
72-73	7.550075878262576E-4	0.0	0.0	1.4400259722610214	0.0012080121405220123
74-75	7.550075878262576E-4	0.0	0.0	1.5281353577603456	0.0012080121405220123
76-77	7.550075878262576E-4	0.0	0.0	1.6282493639061073	0.0012080121405220123
78-79	7.550075878262576E-4	0.0	0.0	1.7476160635414386	0.0012080121405220123
80-81	7.550075878262576E-4	0.0	0.0	1.8540721334249408	0.001283512899304638
82-83	7.550075878262576E-4	0.0	0.0	1.9710228087792283	0.0014345144168698895
84-85	7.550075878262576E-4	0.0	0.0	2.0850289545409932	0.0016610166932177668
86-87	7.550075878262576E-4	0.0	0.0	2.201904129136498	0.0016610166932177668
88-89	7.550075878262576E-4	0.0	0.0	2.3254233705048737	0.0016610166932177668
90-91	7.550075878262576E-4	0.0	0.0	2.4536236589177722	0.0016610166932177668
92-93	7.550075878262576E-4	0.0	0.0	2.589223021691368	0.0016610166932177668
94-95	7.550075878262576E-4	0.0	0.0	2.729654433027052	0.0016610166932177668
96-97	7.550075878262576E-4	0.0	0.0	2.8725018686437798	0.0016610166932177668
98-99	7.550075878262576E-4	0.0	0.0	3.0222198733097265	0.0016610166932177668
100-101	7.550075878262576E-4	0.0	0.0	3.1942106018165486	0.0016610166932177668
102-103	7.550075878262576E-4	0.0	0.0	3.3816789858738083	0.0016610166932177668
104-105	7.550075878262576E-4	0.0	0.0	3.5671088494439367	0.0016610166932177668
106-107	7.550075878262576E-4	0.0	0.0	3.7443091303067595	0.0016610166932177668
108-109	7.550075878262576E-4	0.0	0.0	3.928606482495149	0.0018120182107830183
110-111	7.550075878262576E-4	0.0	0.0	4.1261164674704975	0.0018120182107830183
112-113	7.550075878262576E-4	0.0	0.0	4.33578207460985	0.0018120182107830183
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2000	0.0	42.292248	1
GTATCAA	2935	0.0	38.560966	1
CAACGCA	3630	0.0	30.50783	5
ATCAACG	3615	0.0	30.474333	3
TATCAAC	3760	0.0	30.090996	2
AACGCAG	3660	0.0	29.930143	6
TCAACGC	3705	0.0	29.894793	4
ACGCAGA	4045	0.0	27.230661	7
CGCAGAG	4235	0.0	26.149567	8
GCAGAGT	4410	0.0	25.111885	9
CAGAGTA	4380	0.0	22.292475	10-11
AGAGTAC	4230	0.0	22.027365	10-11
TCTAACG	170	3.909617E-4	21.017117	2
GAGTACA	4655	0.0	20.846828	12-13
CTAACGC	200	4.8743706E-5	20.841974	3
GTACATG	5580	0.0	20.816267	1
TACATGG	5635	0.0	20.289816	2
ATGGGGG	1785	0.0	19.838976	18-19
CCCGTAA	75	0.00471915	19.823963	104-105
CATGGGG	3200	0.0	19.72544	4
>>END_MODULE