FastQCFastQC Report
Sun 1 Jan 2017
ERR1142630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662245
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35930.5425484526119487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33300.5028350534922876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18950.28614787578615164No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.1383173900897704No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.1081170865767201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25950.058.4488751
GTATCAA39100.039.3940662
ATCAACG50450.030.5313743
CAACGCA51250.030.2868716
TATCAAC51300.030.257352
TCAACGC51900.030.1367325
AACGCAG53850.028.824556
GAGTACT28750.026.37424912-13
ACGCAGA58600.026.1836328
CGCAGAG61650.024.9847189
CCGTCTA1200.00129090524.7797289
CAGAGTA64100.023.93698310-11
GTCCGGT1250.001635943723.7921311
GCAGAGT65100.023.2952279
GTACTTT33050.022.7628614-15
AGAGTAC62050.022.04417210-11
CCGCAGA4600.020.6888081
AGTACTT30250.020.54464512-13
GTACATG49300.020.510461
GTGGTAT12200.020.4768331
Produced by FastQC (version 0.11.5)