FastQCFastQC Report
Sun 1 Jan 2017
ERR1142627_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142627_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771595
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26830.347721278650069No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13720.17781349023775428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.054.302941
GTATCAA31650.039.3527531
ATCAACG39650.030.8014983
TATCAAC40650.030.6280022
CAACGCA40350.030.1195055
TCAACGC40600.030.0807744
AACGCAG41900.029.287766
ACGCAGA46550.026.2358657
GTGGTAT10850.025.814911
CGCAGAG47600.025.6571318
GCAGAGT50100.024.3768359
CAGAGTA49950.023.91177410-11
TGGTATC11900.023.5279352
GAGTACA53100.021.59438712-13
AGAGTAC47250.021.11766610-11
GTACATG63450.020.4751641
TACATGG63850.020.1523532
ACATGGG63550.019.6863383
CATGGGT12150.019.1227134
ATGGGTA6000.018.8652925
Produced by FastQC (version 0.11.5)