FastQCFastQC Report
Sun 1 Jan 2017
ERR1142627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771595
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35560.4608635359223427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33050.42833351693569816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.31091440457753095No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.1340081260246632No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.10173731037655764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26100.056.5350151
GTATCAA40050.041.448421
CTAACGC1102.0419613E-532.4474373
TATCAAC53700.031.128192
TCTAACG1152.766938E-531.0366822
ATCAACG54650.030.8047813
TCAACGC55900.030.1139954
CAACGCA55950.030.0870845
GTGGTAT13850.028.7826881
AACGCAG59300.028.387396
TGGTATC14500.027.4870852
ACGCAGA68150.024.6993877
CGCAGAG69300.024.289518
CCGAACA1250.001636358823.7917069
GCAGAGT73750.022.8239069
CAGAGTA73150.021.7507910-11
GAGTACT31050.021.64623812-13
CATGGGG36400.020.7537254
AGAGTAC71300.020.7301510-11
GTACATG61050.020.6612471
Produced by FastQC (version 0.11.5)