FastQCFastQC Report
Sun 1 Jan 2017
ERR1142618_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142618_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573906
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28680.49973340581907144No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9780.17041118231905572No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.033.579991
GTATCAA31450.027.4674452
GTACTAG2809.276846E-1125.5347841
CATGGGG28700.024.2870584
TAACCTA1250.001621274323.8282956
ATGGGGG19600.023.706725
AACGCAG37200.023.0616577
TATCTCG1300.00203678822.911825
TATCAAC38150.022.7997073
ATCAACG37500.022.5594254
CAACGCA37700.022.4377846
ATAGGAC1602.5866152E-422.340983
TGGGGGG11100.022.0036056
TCAACGC39100.021.4820295
ACGCAGA40250.021.3141298
ACATGGG51750.020.9523123
GTACATG54350.020.7191141
CGCAGAG41650.020.4546459
TACATGG55550.020.1625162
GTACAAA4254.0017767E-1119.6267361
Produced by FastQC (version 0.11.5)