##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142618_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	573906
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16308768334884	33.0	33.0	33.0	27.0	33.0
2	31.24949033465411	33.0	33.0	33.0	27.0	33.0
3	31.366305631932754	33.0	33.0	33.0	27.0	33.0
4	31.28828588653891	33.0	33.0	33.0	27.0	33.0
5	31.35718741396675	33.0	33.0	33.0	27.0	33.0
6	34.54510320505449	37.0	37.0	37.0	27.0	37.0
7	34.67497813230738	37.0	37.0	37.0	27.0	37.0
8	34.68001031527811	37.0	37.0	37.0	27.0	37.0
9	34.55792934731472	37.0	37.0	37.0	27.0	37.0
10-11	34.60747753116364	37.0	37.0	37.0	27.0	37.0
12-13	34.61823016312776	37.0	37.0	37.0	27.0	37.0
14-15	34.58753436973999	37.0	37.0	37.0	27.0	37.0
16-17	34.585175098361056	37.0	37.0	37.0	27.0	37.0
18-19	34.60952054866128	37.0	37.0	37.0	27.0	37.0
20-21	34.67829574878116	37.0	37.0	37.0	27.0	37.0
22-23	34.73010911194516	37.0	37.0	37.0	27.0	37.0
24-25	34.78758193850561	37.0	37.0	37.0	27.0	37.0
26-27	34.77717256833	37.0	37.0	37.0	27.0	37.0
28-29	34.81316278275537	37.0	37.0	37.0	27.0	37.0
30-31	34.87332420291825	37.0	37.0	37.0	27.0	37.0
32-33	34.884564719657924	37.0	37.0	37.0	27.0	37.0
34-35	34.88628974082864	37.0	37.0	37.0	27.0	37.0
36-37	34.84285231379354	37.0	37.0	37.0	27.0	37.0
38-39	34.81502022979373	37.0	37.0	37.0	27.0	37.0
40-41	34.80233783929772	37.0	37.0	37.0	27.0	37.0
42-43	34.7930375357637	37.0	37.0	37.0	27.0	37.0
44-45	34.818575690095585	37.0	37.0	37.0	27.0	37.0
46-47	34.802368332096194	37.0	37.0	37.0	27.0	37.0
48-49	34.785024028325196	37.0	37.0	37.0	27.0	37.0
50-51	34.763188570950646	37.0	37.0	37.0	27.0	37.0
52-53	34.737041431872115	37.0	37.0	37.0	27.0	37.0
54-55	34.688164089589584	37.0	37.0	37.0	27.0	37.0
56-57	34.635523935975584	37.0	37.0	37.0	27.0	37.0
58-59	34.58034068296899	37.0	37.0	37.0	27.0	37.0
60-61	34.54901673793269	37.0	37.0	37.0	27.0	37.0
62-63	34.592127456412726	37.0	37.0	37.0	27.0	37.0
64-65	34.53842615341188	37.0	37.0	37.0	27.0	37.0
66-67	34.51588587678121	37.0	37.0	37.0	27.0	37.0
68-69	34.48424393541799	37.0	37.0	37.0	27.0	37.0
70-71	34.45429477998139	37.0	37.0	37.0	27.0	37.0
72-73	34.421980254606154	37.0	37.0	37.0	27.0	37.0
74-75	34.33197422574429	37.0	37.0	37.0	27.0	37.0
76-77	34.28471997156329	37.0	37.0	37.0	27.0	37.0
78-79	34.2183266946155	37.0	37.0	37.0	27.0	37.0
80-81	34.15138890340927	37.0	37.0	37.0	27.0	37.0
82-83	34.128202179450994	37.0	37.0	37.0	27.0	37.0
84-85	34.09193665861657	37.0	37.0	37.0	27.0	37.0
86-87	34.03605904102763	37.0	37.0	37.0	27.0	37.0
88-89	33.977770749913745	37.0	37.0	37.0	27.0	37.0
90-91	33.89765048631658	37.0	37.0	37.0	24.5	37.0
92-93	33.7696042557492	37.0	35.0	37.0	22.0	37.0
94-95	33.70791296832582	37.0	33.0	37.0	22.0	37.0
96-97	33.62108603151039	37.0	33.0	37.0	22.0	37.0
98-99	33.572758430823164	37.0	33.0	37.0	22.0	37.0
100-101	33.45449341878287	37.0	33.0	37.0	22.0	37.0
102-103	33.385569239561875	37.0	33.0	37.0	22.0	37.0
104-105	33.26128843399442	37.0	33.0	37.0	18.0	37.0
106-107	33.16958526309186	37.0	33.0	37.0	14.0	37.0
108-109	33.04259146968319	37.0	33.0	37.0	14.0	37.0
110-111	32.975150111690766	37.0	33.0	37.0	14.0	37.0
112-113	32.91030586890536	37.0	33.0	37.0	14.0	37.0
114-115	32.725988228037345	37.0	33.0	37.0	14.0	37.0
116-117	32.5332876812579	37.0	33.0	37.0	14.0	37.0
118-119	32.41348147606054	37.0	33.0	37.0	14.0	37.0
120-121	32.34266238722021	37.0	33.0	37.0	14.0	37.0
122-123	32.147430066944764	37.0	33.0	37.0	14.0	37.0
124-125	30.553435580042724	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	958.0
1	111.0
2	28.0
3	20.0
4	26.0
5	12.0
6	22.0
7	15.0
8	9.0
9	16.0
10	13.0
11	22.0
12	19.0
13	16.0
14	49.0
15	217.0
16	294.0
17	603.0
18	1357.0
19	2607.0
20	3762.0
21	4410.0
22	4608.0
23	5072.0
24	5707.0
25	6728.0
26	7788.0
27	9489.0
28	11267.0
29	13252.0
30	16050.0
31	19627.0
32	24516.0
33	32853.0
34	48385.0
35	92535.0
36	261443.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.39133673624627	20.328295413411954	12.472217996268336	23.80814985407344
2	17.073413704031235	23.452502874959364	36.449416444418034	23.024666976591366
3	19.584162672151518	28.11008609978628	28.480907586462394	23.82484364159981
4	14.551571074062425	19.849708154206425	34.36248296109888	31.23623781063227
5	14.852577189842265	38.416104989372414	31.264333258753773	15.466984562031547
6	28.68785399622404	36.50863575973708	18.59747570099993	16.20603454303895
7	25.584216921180758	34.30739241789845	21.66537005795404	18.443020602966754
8	25.386777709017295	34.35949611386736	20.714852613655832	19.538873563459514
9	26.458827622742586	19.005715544567785	19.84932222977923	34.6861346029104
10-11	24.86883580743121	27.964065993814607	25.574613675342178	21.592484523412
12-13	25.58030367929229	26.82736435396033	25.26421147407712	22.328120492670262
14-15	24.277196948043493	26.938437292677875	23.757378373204737	25.026987386073895
16-17	23.580987459746957	28.76211959456036	24.327689025487622	23.32920392020506
18-19	23.669563834815115	28.122420132770614	25.76053939918632	22.447476633227947
20-21	25.35931179835391	26.840143698516112	25.28707822625092	22.513466276879054
22-23	25.683659084914346	26.41050813054286	24.90204743995766	23.00378534458514
24-25	24.477214910193183	27.077844217248042	25.060935192551714	23.384005680007057
26-27	23.76268838812167	27.467876752226772	25.19783099848652	23.571603861165038
28-29	23.65344731981854	27.451589945553696	25.487942785537456	23.407019949090305
30-31	24.509532537594428	26.917444635486206	25.449818937235356	23.123203889684007
32-33	23.991246951408527	27.323238453337012	25.135150947300954	23.550363647953503
34-35	24.04895041992809	27.34340136078185	25.396720045900846	23.210928173389213
36-37	24.062992015969805	27.485829503449732	25.087738754637996	23.363439725942467
38-39	23.915034500099967	27.36329831956618	25.147835744076747	23.5738314362571
40-41	23.62975835960492	27.236374433450646	25.496860508780973	23.63700669816346
42-43	23.795590482427418	27.34075529360402	25.21143855053482	23.652215673433748
44-45	23.87130852340936	27.435032194696063	25.21060787951544	23.483051402379136
46-47	23.775832381591034	27.074710897362014	25.25452557829259	23.89493114275436
48-49	23.73522272862207	27.375189899942377	25.365568302862034	23.524019068573523
50-51	23.44293368886002	27.57392327014702	25.298884264364474	23.684258776628486
52-53	23.538084224795096	27.28746410279701	25.24018809785001	23.93426357455788
54-55	23.254386194730454	27.0648082649969	25.418446033866925	24.262359506405723
56-57	23.546816011681187	27.013795434241356	25.0585762329391	24.380812321138357
58-59	23.542115310387228	27.13667936404807	25.398692536489687	23.92251278907501
60-61	23.781425633097957	26.992877511539653	25.71082958867738	23.51486726668501
62-63	23.290561345230547	26.959887944027905	25.647648937860986	24.10190177288057
64-65	23.183481054721103	26.706000815677065	25.835654170389176	24.274863959212652
66-67	23.67859616152107	26.57924405875875	25.53967420872963	24.20248557099055
68-69	23.478739995214816	26.672674340422038	25.4205808232958	24.42800484106734
70-71	23.307633800430857	26.79030036929434	25.563307039995387	24.33875879027941
72-73	23.6265434793999	26.932776778385175	25.532162006392234	23.90851773582269
74-75	23.5193447833262	26.766264723602294	25.835043264906965	23.879347228164537
76-77	23.416426602304092	26.33562089420082	25.771080328926743	24.476872174568342
78-79	23.45716962990585	26.549074437138838	25.680930793904743	24.31282513905057
80-81	23.26729344744271	26.843209627520377	25.7759009864506	24.11359593858631
82-83	23.397928269316257	26.606394750194973	26.14212865480733	23.85354832568144
84-85	23.256390622285124	26.991795241641793	25.51245906133792	24.23935507473516
86-87	22.87983027466867	27.269988999284077	25.69627547189579	24.15390525415146
88-89	23.170871194162643	26.407017121083932	25.875022810358388	24.547088874395033
90-91	23.572129504492707	26.358550460445674	25.775703415015734	24.29361662004589
92-93	23.583320671546257	26.64598865853169	25.632434433773614	24.138256236148443
94-95	22.834230435105273	26.759738247093694	25.961819697822946	24.444211619978084
96-97	23.02249529456544	26.687059793065636	25.782027509768795	24.508417402600124
98-99	22.72539586514178	26.94100280307177	25.68018793064165	24.6534134011448
100-101	23.20460169421253	27.104869224424245	25.43396193477151	24.256567146591717
102-103	23.12252464119573	26.996589384404402	25.858175307562103	24.022710666837767
104-105	23.270607152303544	27.04866873086636	25.743805873279555	23.93691824355054
106-107	23.1725308280942	26.90385196229571	25.8509042849436	24.072712924666494
108-109	23.07835332320746	27.00282826166658	25.69772516978299	24.22109324534297
110-111	22.720122903955097	27.78580843393476	25.559309014411536	23.934759647698606
112-113	22.752261704824388	27.70714981546723	25.293122531852415	24.247465947855964
114-115	22.864871187020597	27.810004783135433	25.30400840714607	24.0211156226979
116-117	22.804230263933203	27.646424658789844	25.37067185774581	24.178673219531145
118-119	22.64085733212194	27.195717877755637	25.80689080954837	24.356533980574053
120-121	22.78763630206274	27.242868826202816	25.98556876135214	23.983926110382306
122-123	22.78456595569001	27.654161076930194	25.744151397266823	23.81712157011297
124-125	22.74695379792297	27.565805034339323	25.44845348677877	24.238787680958936
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2673.0
1	1766.0
2	688.5
3	443.5
4	330.0
5	273.5
6	240.0
7	215.0
8	207.0
9	211.5
10	199.0
11	172.5
12	147.5
13	138.5
14	158.0
15	176.0
16	194.5
17	208.0
18	250.5
19	286.0
20	303.5
21	372.0
22	455.5
23	578.5
24	750.0
25	1007.5
26	1324.0
27	1683.0
28	2141.5
29	2945.0
30	3971.0
31	5003.5
32	6299.5
33	7582.5
34	8784.0
35	9895.0
36	11013.5
37	12480.5
38	13548.0
39	13953.0
40	14244.5
41	14744.0
42	15270.5
43	16287.0
44	18018.0
45	19591.5
46	21314.0
47	22971.0
48	24411.5
49	25297.0
50	25447.0
51	25121.0
52	24211.0
53	23852.5
54	23136.5
55	21837.5
56	20580.0
57	18259.5
58	15791.0
59	13595.5
60	11494.0
61	9638.5
62	7909.5
63	6267.5
64	4654.5
65	3497.0
66	2730.0
67	2122.0
68	1728.0
69	1456.0
70	1248.5
71	1092.5
72	931.0
73	834.5
74	659.0
75	526.0
76	426.0
77	288.0
78	214.0
79	154.0
80	105.5
81	70.5
82	48.5
83	37.0
84	25.5
85	10.0
86	3.5
87	4.5
88	3.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3559816415928741
2	0.30039762609207776
3	0.2897687077674741
4	0.2937763327095378
5	0.315382658484142
6	0.3251403539952536
7	0.3312389136896983
8	0.3261858213714441
9	0.3409966092008099
10-11	0.33332984844207936
12-13	0.3347238049436667
14-15	0.3274926555916823
16-17	0.27687461012779097
18-19	0.27277986290437806
20-21	0.25396145013294863
22-23	0.2500409474722341
24-25	0.2389764177408844
26-27	0.24124159705596385
28-29	0.23061267873136018
30-31	0.22904447766707442
32-33	0.2274762766027886
34-35	0.23827943949009073
36-37	0.23096116785675702
38-39	0.21371095614961333
40-41	0.23714684983255097
42-43	0.2232072848166773
44-45	0.21231699964802597
46-47	0.22163908375239152
48-49	0.21536627949524834
50-51	0.21510491265120074
52-53	0.2505636811603294
54-55	0.23888929545953516
56-57	0.2372339721139002
58-59	0.23470742595477306
60-61	0.24803713500120228
62-63	0.23261649120239203
64-65	0.23967339599167808
66-67	0.2372339721139002
68-69	0.2273891543214394
70-71	0.23130965698215386
72-73	0.23279073576509046
74-75	0.22146483918969306
76-77	0.22939296679247126
78-79	0.23740821667659862
80-81	0.22608232010120125
82-83	0.242112819869456
84-85	0.21789282565437545
86-87	0.21188138824127992
88-89	0.21771858109167702
90-91	0.2090934752381052
92-93	0.22948008907382045
94-95	0.21405944527501017
96-97	0.20282067098096201
98-99	0.19890016832024757
100-101	0.18705153805675495
102-103	0.19785470094405705
104-105	0.19611225531707285
106-107	0.19741908953731102
108-109	0.19445693197143785
110-111	0.1918432635309615
112-113	0.19306297546985046
114-115	0.18469923646032624
116-117	0.19663498900516807
118-119	0.17642261973215126
120-121	0.1832181576773897
122-123	0.18391513592818337
124-125	0.17781657623373864
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	573906.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.84680472155316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.62439022260105	63.96934428892038
2	8.890026501279639	13.130001020188603
3	2.13500126137946	4.72989063688076
4	0.8060079042331987	2.3808443323174937
5	0.4088662107165331	1.5096731610012615
6	0.24790087404808858	1.0984012461678927
7	0.1563655885815352	0.8082969359605948
8	0.11843475347089186	0.6996822489448192
9	0.07808397379811259	0.5189626768456885
>10	0.48138700526865963	6.583865878889263
>50	0.036060320864570505	1.812232675515504
>100	0.016920609670759403	1.9709846103426385
>500	2.773870437829312E-4	0.20033495623727446
>1k	2.773870437829312E-4	0.5874853317878355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2868	0.49973340581907144	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	978	0.17041118231905572	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.74244562698421E-4
7	0.0	0.0	0.0	1.74244562698421E-4	1.74244562698421E-4
8	0.0	0.0	0.0	1.74244562698421E-4	1.74244562698421E-4
9	0.0	0.0	0.0	1.74244562698421E-4	1.74244562698421E-4
10-11	0.0	0.0	0.0	2.613668440476315E-4	1.74244562698421E-4
12-13	0.0	0.0	0.0	5.22733688095263E-4	1.74244562698421E-4
14-15	0.0	0.0	0.0	5.22733688095263E-4	1.74244562698421E-4
16-17	0.0	0.0	0.0	5.22733688095263E-4	1.74244562698421E-4
18-19	0.0	0.0	0.0	6.098559694444736E-4	1.74244562698421E-4
20-21	0.0	0.0	0.0	7.841005321428945E-4	1.74244562698421E-4
22-23	0.0	0.0	0.0	0.0021780570337302622	1.74244562698421E-4
24-25	0.0	0.0	0.0	0.005750070569047893	1.74244562698421E-4
26-27	0.0	0.0	0.0	0.009931940073809997	3.48489125396842E-4
28-29	1.74244562698421E-4	0.0	0.0	0.01873129049008026	3.48489125396842E-4
30-31	1.74244562698421E-4	0.0	0.0	0.036765602729366825	3.48489125396842E-4
32-33	1.74244562698421E-4	0.0	0.0	0.0655159555746063	3.48489125396842E-4
34-35	1.74244562698421E-4	0.0	0.0	0.09644436545357601	5.22733688095263E-4
36-37	1.74244562698421E-4	0.0	0.0	0.14802075601230863	5.22733688095263E-4
38-39	1.74244562698421E-4	0.0	0.0	0.21632462459008966	5.22733688095263E-4
40-41	1.74244562698421E-4	0.0	0.0	0.2898558300488233	5.22733688095263E-4
42-43	1.74244562698421E-4	0.0	0.0	0.357201353531763	5.22733688095263E-4
44-45	1.74244562698421E-4	0.0	0.0	0.42689917861113147	5.22733688095263E-4
46-47	1.74244562698421E-4	0.0	0.0	0.5160252724313737	5.22733688095263E-4
48-49	1.74244562698421E-4	0.0	0.0	0.6053256108143146	5.22733688095263E-4
50-51	1.74244562698421E-4	0.0	0.0	0.68748192212662	5.22733688095263E-4
52-53	1.74244562698421E-4	0.0	0.0	0.7688541329067826	5.22733688095263E-4
54-55	1.74244562698421E-4	0.0	0.0	0.84970360999885	6.098559694444736E-4
56-57	1.74244562698421E-4	0.0	0.0	0.936216035378616	6.96978250793684E-4
58-59	1.74244562698421E-4	0.0	0.0	1.0341414796151285	6.96978250793684E-4
60-61	1.74244562698421E-4	0.0	0.0	1.1344192254480698	6.96978250793684E-4
62-63	1.74244562698421E-4	0.0	0.0	1.2304279794949	6.96978250793684E-4
64-65	1.74244562698421E-4	0.0	0.0	1.3121486794004593	6.96978250793684E-4
66-67	1.74244562698421E-4	0.0	0.0	1.4107711018877656	7.841005321428945E-4
68-69	1.74244562698421E-4	0.0	0.0	1.5198481981369771	8.71222813492105E-4
70-71	1.74244562698421E-4	0.0	0.0	1.6480050740016656	8.71222813492105E-4
72-73	1.74244562698421E-4	0.0	0.0	1.767275477168735	0.001045467376190526
74-75	1.74244562698421E-4	0.0	0.0	1.873651782696121	0.001045467376190526
76-77	1.74244562698421E-4	0.0	0.0	1.9842970800096182	0.001045467376190526
78-79	1.74244562698421E-4	0.0	0.0	2.112018344467561	0.001045467376190526
80-81	1.74244562698421E-4	0.0	0.0	2.2475806142469326	0.001045467376190526
82-83	1.74244562698421E-4	0.0	0.0	2.3922036012866217	0.001045467376190526
84-85	1.74244562698421E-4	0.0	0.0	2.5295954389743267	0.001045467376190526
86-87	1.74244562698421E-4	0.0	0.0	2.668642600007667	0.001045467376190526
88-89	1.74244562698421E-4	0.0	0.0	2.824155872216007	0.001045467376190526
90-91	1.74244562698421E-4	0.0	0.0	2.985941948681491	0.001045467376190526
92-93	1.74244562698421E-4	0.0	0.0	3.1367506176969746	0.001045467376190526
94-95	1.74244562698421E-4	0.0	0.0	3.297839715911665	0.001045467376190526
96-97	1.74244562698421E-4	0.0	0.0	3.4723456454541335	0.001045467376190526
98-99	1.74244562698421E-4	0.0	0.0	3.6485940206235865	0.001045467376190526
100-101	1.74244562698421E-4	0.0	0.0	3.845054765066056	0.001045467376190526
102-103	1.74244562698421E-4	0.0	0.0	4.065648381442257	0.001045467376190526
104-105	1.74244562698421E-4	0.0	0.0	4.284151063066076	0.0011325896575397365
106-107	1.74244562698421E-4	0.0	0.0	4.509623527197833	0.001219711938888947
108-109	1.74244562698421E-4	0.0	0.0	4.739103616271654	0.001219711938888947
110-111	1.74244562698421E-4	0.0	0.0	4.966492770593094	0.001393956501587368
112-113	1.74244562698421E-4	0.0	0.0	5.222370910915725	0.0017424456269842098
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2555	0.0	33.57999	1
GTATCAA	3145	0.0	27.467445	2
GTACTAG	280	9.276846E-11	25.534784	1
CATGGGG	2870	0.0	24.287058	4
TAACCTA	125	0.0016212743	23.828295	6
ATGGGGG	1960	0.0	23.70672	5
AACGCAG	3720	0.0	23.061657	7
TATCTCG	130	0.002036788	22.91182	5
TATCAAC	3815	0.0	22.799707	3
ATCAACG	3750	0.0	22.559425	4
CAACGCA	3770	0.0	22.437784	6
ATAGGAC	160	2.5866152E-4	22.34098	3
TGGGGGG	1110	0.0	22.003605	6
TCAACGC	3910	0.0	21.482029	5
ACGCAGA	4025	0.0	21.314129	8
ACATGGG	5175	0.0	20.952312	3
GTACATG	5435	0.0	20.719114	1
CGCAGAG	4165	0.0	20.454645	9
TACATGG	5555	0.0	20.162516	2
GTACAAA	425	4.0017767E-11	19.626736	1
>>END_MODULE