FastQCFastQC Report
Sun 1 Jan 2017
ERR1142618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573906
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30440.5304004488539935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.5155896610246278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21400.3728833641746209No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.151070035859531No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.1221454384515931No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.12005450369921207No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.10594069412063996No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30200.039.5862921
GTATCAA41750.032.0539631
TATCAAC51850.026.1519412
ATCAACG52200.025.8626613
CAACGCA52450.025.6265
TCAACGC53300.025.4404934
AACGCAG55850.024.598396
GAGTACT27550.022.66655712-13
ACGCAGA60550.022.2961357
GTAAGTA4050.022.0269372
CGCAGAG61850.021.7313468
TAGAACG1703.942504E-420.9903747
CATGGGG26950.020.9644154
GCAGAGT65550.020.504719
CAGAGTA65050.019.70234110-11
GTACTTT31800.019.5437114-15
AGTACTT28800.019.5144912-13
ATGGGAG4900.019.4196665
TGTAAGT4304.9112714E-1119.36491
TAACACT2158.582766E-519.3632144
Produced by FastQC (version 0.11.5)