##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142618_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	573906
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.753109394221354	33.0	33.0	33.0	14.0	33.0
2	28.91505054834764	33.0	27.0	33.0	14.0	33.0
3	30.67247249549578	33.0	27.0	33.0	27.0	33.0
4	31.447974058469505	33.0	33.0	33.0	27.0	33.0
5	31.847314717044256	33.0	33.0	33.0	27.0	33.0
6	34.960526636766296	37.0	37.0	37.0	27.0	37.0
7	35.8216955389907	37.0	37.0	37.0	33.0	37.0
8	35.970293044505546	37.0	37.0	37.0	33.0	37.0
9	36.01707770959007	37.0	37.0	37.0	33.0	37.0
10-11	36.07953828675776	37.0	37.0	37.0	35.0	37.0
12-13	36.14957240384314	37.0	37.0	37.0	37.0	37.0
14-15	36.1467548692643	37.0	37.0	37.0	37.0	37.0
16-17	36.196920750088	37.0	37.0	37.0	37.0	37.0
18-19	36.20234933943887	37.0	37.0	37.0	37.0	37.0
20-21	36.21744588835105	37.0	37.0	37.0	37.0	37.0
22-23	36.206324729136824	37.0	37.0	37.0	37.0	37.0
24-25	36.19432102121253	37.0	37.0	37.0	37.0	37.0
26-27	36.14261220478615	37.0	37.0	37.0	37.0	37.0
28-29	36.12839471969278	37.0	37.0	37.0	37.0	37.0
30-31	36.096656943820065	37.0	37.0	37.0	37.0	37.0
32-33	36.03193989956543	37.0	37.0	37.0	37.0	37.0
34-35	35.92198199705179	37.0	37.0	37.0	35.0	37.0
36-37	35.84100270776051	37.0	37.0	37.0	33.0	37.0
38-39	35.82477879652765	37.0	37.0	37.0	33.0	37.0
40-41	35.72091074148031	37.0	37.0	37.0	33.0	37.0
42-43	35.673416029802794	37.0	37.0	37.0	33.0	37.0
44-45	35.58677553466944	37.0	37.0	37.0	33.0	37.0
46-47	35.45294699828892	37.0	37.0	37.0	33.0	37.0
48-49	35.30582882911139	37.0	37.0	37.0	33.0	37.0
50-51	35.221241806149436	37.0	37.0	37.0	33.0	37.0
52-53	35.133310158806495	37.0	37.0	37.0	33.0	37.0
54-55	35.091048882569616	37.0	37.0	37.0	33.0	37.0
56-57	35.07854160785913	37.0	37.0	37.0	33.0	37.0
58-59	35.04285632141849	37.0	37.0	37.0	33.0	37.0
60-61	35.019246183172854	37.0	37.0	37.0	33.0	37.0
62-63	35.000886904824135	37.0	37.0	37.0	30.0	37.0
64-65	34.97035577254812	37.0	37.0	37.0	27.0	37.0
66-67	34.95005453854812	37.0	37.0	37.0	27.0	37.0
68-69	34.92175199422902	37.0	37.0	37.0	27.0	37.0
70-71	34.81343460427317	37.0	37.0	37.0	27.0	37.0
72-73	34.82568399703087	37.0	37.0	37.0	27.0	37.0
74-75	34.75803703045446	37.0	37.0	37.0	27.0	37.0
76-77	34.65190379609204	37.0	37.0	37.0	27.0	37.0
78-79	34.65170777095901	37.0	37.0	37.0	27.0	37.0
80-81	34.64478590570581	37.0	37.0	37.0	27.0	37.0
82-83	34.56202060964688	37.0	37.0	37.0	27.0	37.0
84-85	34.50323397908368	37.0	37.0	37.0	27.0	37.0
86-87	34.39275160043631	37.0	37.0	37.0	27.0	37.0
88-89	34.32153784766146	37.0	37.0	37.0	27.0	37.0
90-91	34.25001045467376	37.0	37.0	37.0	27.0	37.0
92-93	34.091914006823416	37.0	37.0	37.0	27.0	37.0
94-95	34.12971984959209	37.0	37.0	37.0	27.0	37.0
96-97	34.12779183350584	37.0	37.0	37.0	27.0	37.0
98-99	34.095981746139614	37.0	37.0	37.0	27.0	37.0
100-101	33.95345927730325	37.0	37.0	37.0	22.0	37.0
102-103	33.88639428756626	37.0	37.0	37.0	22.0	37.0
104-105	33.74698818273376	37.0	33.0	37.0	22.0	37.0
106-107	33.72177325206567	37.0	33.0	37.0	22.0	37.0
108-109	33.61613138737006	37.0	33.0	37.0	22.0	37.0
110-111	33.54095531323945	37.0	33.0	37.0	22.0	37.0
112-113	33.4058661174478	37.0	33.0	37.0	22.0	37.0
114-115	33.38029224298056	37.0	33.0	37.0	22.0	37.0
116-117	33.22923788913167	37.0	33.0	37.0	14.0	37.0
118-119	33.09927671082024	37.0	33.0	37.0	14.0	37.0
120-121	33.01629012416667	37.0	33.0	37.0	14.0	37.0
122-123	32.94036479841647	37.0	33.0	37.0	14.0	37.0
124-125	31.61605733343091	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	77.0
16	27.0
17	26.0
18	80.0
19	362.0
20	1155.0
21	2673.0
22	4472.0
23	6384.0
24	5792.0
25	4566.0
26	4338.0
27	5049.0
28	6330.0
29	8068.0
30	10778.0
31	15027.0
32	21206.0
33	31927.0
34	52458.0
35	108530.0
36	284579.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.1308573291015	20.383025967891772	12.891919260580012	23.594197442426715
2	17.22128710973574	23.551592072569374	35.7096806794144	23.517440138280485
3	21.17165937160222	26.457560846413337	29.104170732387296	23.266609049597147
4	15.761897045319506	18.20203065674861	34.37096512825338	31.665107169678496
5	15.832687164028265	36.60791608221016	31.669382029813814	15.89001472394776
6	29.496090734683992	34.86346758019352	18.702441047372943	16.938000637749546
7	25.40642544249407	32.67834802214997	22.956895380079665	18.9583311552763
8	26.142434475332198	32.02475666746819	21.448111711674038	20.384697145525575
9	26.772502814049687	16.115879603976957	21.266374632779584	35.845242949193775
10-11	25.466452694343673	25.62396977902305	26.717615776799686	22.191961749833595
12-13	26.56523890671992	24.43300819297934	26.88462919014612	22.117123710154623
14-15	24.612218725714662	24.688450721895222	26.006523716427427	24.69280683596268
16-17	23.335180325697937	26.536924165281423	26.810226761873896	23.317668747146744
18-19	23.026418960596335	25.34665955748851	29.45160008781926	22.1753213940959
20-21	24.394912393961015	24.222148458497887	29.336239740235527	22.04669940730557
22-23	24.42246639693608	23.949740898335268	29.213669137454566	22.414123567274082
24-25	23.57224005324914	24.61823016312776	29.18003993685377	22.62948984676933
26-27	23.041839604395147	24.90190031120079	29.311071848002985	22.745188236401084
28-29	22.82131792536008	24.873323320009337	29.547989029485873	22.75736972514471
30-31	23.486250361557467	24.415583736709497	29.634644000933953	22.463521900799087
32-33	23.245156641915163	24.82686579387337	29.140823952417172	22.7871536117943
34-35	23.224273661540394	24.875066648545232	29.436702177708547	22.46395751220583
36-37	23.302825828429043	24.860581719020217	29.191108245738604	22.64548420681213
38-39	23.197527121166182	24.844922339198405	29.116527793750198	22.841022745885216
40-41	22.946853379590465	24.711727872854457	29.357973212799937	22.98344553475514
42-43	23.241049624455805	24.778337011380895	29.033517134067665	22.947096230095635
44-45	23.255027826856665	24.99886741034246	28.776663774206924	22.969440988593952
46-47	23.217216756751107	24.913400452338884	28.5314145522089	23.337968238701112
48-49	23.30852090760508	25.276177631876994	28.478879816555324	22.9364216439626
50-51	23.071635424616574	25.42219457541827	28.303241297355314	23.202928702609835
52-53	23.097510742177292	25.332807114753987	28.1063449415061	23.463337201562627
54-55	22.821245988018944	25.2425484312762	28.221346352887057	23.714859227817797
56-57	23.1901217272515	25.027269274062302	27.795928253058864	23.986680745627336
58-59	23.035479677856653	25.266071447240492	28.225876711517216	23.472572163385642
60-61	23.278115231414205	25.17807794307779	28.561123250148977	22.98268357535903
62-63	22.816889873951485	25.03406481200754	28.60285482291525	23.546190491125724
64-65	22.65945991155346	24.835861621938086	28.72090551414343	23.78377295236502
66-67	23.04488888424237	24.903381389983725	28.538297212435488	23.513432513338422
68-69	22.810007213724894	24.84239579303928	28.604161657135492	23.743435336100337
70-71	22.860102525500693	25.02125783664921	28.684227033695414	23.434412604154687
72-73	23.033998599073715	25.070133436486113	28.699212066087476	23.19665589835269
74-75	22.98053696599134	25.05480979367109	28.799223117485173	23.165430122852392
76-77	22.799843761443253	24.838007121409216	28.782245999002587	23.579903118144944
78-79	22.79425793967822	25.04532540636045	28.73373752298941	23.426679130971912
80-81	22.763745282328465	25.323920642056365	28.657219126477155	23.25511494913801
82-83	22.872561011733627	25.039205026607142	28.929911867100188	23.15832209455904
84-85	22.593051362940194	25.40967530197727	28.440126256440745	23.557147078641794
86-87	22.254432836797452	25.759525419292927	28.555974787899935	23.430066956009686
88-89	22.64341276032804	24.988001305457967	28.581763031454905	23.786822902759088
90-91	22.8756221247571	24.962753154321813	28.481871748801517	23.67975297211957
92-93	22.991438977421787	25.207746085791634	28.27998102202004	23.52083391476654
94-95	22.382334312301634	25.39033130134247	28.448162452398357	23.77917193395754
96-97	22.42440399647329	25.295518778336522	28.325631723662063	23.954445501528124
98-99	22.323603516952254	25.377239478242082	28.062348189424753	24.23680881538092
100-101	22.62940272448798	25.643049558638527	27.708457482584254	24.01909023428924
102-103	22.722536443250288	25.57596540199963	27.988294250277917	23.71320390447216
104-105	22.871144798745426	25.588081547307894	27.668234884126154	23.872538769820526
106-107	22.80408289859315	25.46993758559764	27.712116618400923	24.013862897408288
108-109	22.703535782988098	25.594437397195353	27.54136580333993	24.160661016476624
110-111	22.40924471951853	26.198018490832993	27.40039309573345	23.992343693915032
112-113	22.41029018689472	26.250291859642523	27.1961784682509	24.143239485211865
114-115	22.475631897906627	26.13250253525839	27.276069600248125	24.115795966586862
116-117	22.57884369263527	26.039959610004797	27.196685162775342	24.184511534584587
118-119	22.32761114189432	25.775388304007972	27.625517070739807	24.271483483357905
120-121	22.33810473858914	25.844608428224188	27.84258718777498	23.974699645411697
122-123	22.423619895941147	26.10174836994212	27.590232546793374	23.88439918732336
124-125	22.343129114912173	25.97405312914105	27.5024459687038	24.180371787242976
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	409.0
1	368.0
2	509.5
3	718.0
4	545.5
5	425.0
6	712.0
7	1520.0
8	2220.0
9	2334.5
10	1991.5
11	1432.5
12	1198.5
13	1169.5
14	1087.5
15	1016.0
16	1022.5
17	994.5
18	927.5
19	854.5
20	852.5
21	897.0
22	920.0
23	971.0
24	1017.0
25	1138.5
26	1366.5
27	1628.0
28	2028.0
29	2756.5
30	3743.5
31	4700.0
32	5801.0
33	6950.5
34	8065.5
35	9148.5
36	10314.0
37	11869.0
38	12901.0
39	13366.0
40	13710.0
41	13975.0
42	14479.5
43	15605.5
44	17398.0
45	18996.5
46	20572.5
47	22297.0
48	24092.0
49	25001.0
50	25036.0
51	24492.5
52	23470.5
53	23201.5
54	22826.0
55	21726.0
56	20415.0
57	18296.0
58	16005.0
59	13799.0
60	11566.0
61	9541.5
62	7604.5
63	5956.0
64	4512.5
65	3481.5
66	2661.0
67	2038.0
68	1666.0
69	1398.5
70	1186.0
71	1016.5
72	889.5
73	813.0
74	623.5
75	505.5
76	442.0
77	318.0
78	211.0
79	129.5
80	83.5
81	63.5
82	50.0
83	27.5
84	15.5
85	13.0
86	7.5
87	2.5
88	1.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02770488546904894
2	0.0
3	3.48489125396842E-4
4	5.22733688095263E-4
5	0.001916690189682631
6	0.002265179315079473
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.613668440476315E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	6.96978250793684E-4
30-31	0.0
32-33	2.613668440476315E-4
34-35	0.0
36-37	6.098559694444734E-4
38-39	0.0
40-41	0.0021780570337302627
42-43	9.583450948413155E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.05776207253452655
76-77	0.0738796945841305
78-79	8.71222813492105E-5
80-81	0.0
82-83	0.0
84-85	0.05427718128055813
86-87	0.13390694643373655
88-89	0.16152470962143625
90-91	0.06403487679166972
92-93	0.10628918324603681
94-95	4.356114067460525E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.001045467376190526
106-107	0.0
108-109	3.48489125396842E-4
110-111	0.0
112-113	0.0
114-115	0.0
116-117	2.613668440476315E-4
118-119	0.0
120-121	1.74244562698421E-4
122-123	0.0
124-125	4.356114067460525E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	573906.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.18712515327721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.78034514231508	58.65708349899391
2	10.059829022151648	13.920212991523528
3	2.3885580307698455	4.95772390232216
4	0.9259056746196598	2.5624300716015984
5	0.45872695545232195	1.586899963903081
6	0.29825849131565685	1.2381388540010396
7	0.19754268034935218	0.9567187103911126
8	0.14210769653497446	0.7865618388327373
9	0.0882800089013944	0.549705602195388
>10	0.5881323232809971	7.829069544769903
>50	0.04918521437180897	2.29640621057892
>100	0.020786607032194142	2.320105433672279
>500	0.0014638455656474521	0.6895092453483811
>1k	8.783073393884714E-4	1.649434131866001
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3044	0.5304004488539935	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2959	0.5155896610246278	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2140	0.3728833641746209	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	867	0.151070035859531	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	701	0.1221454384515931	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	689	0.12005450369921207	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.10594069412063996	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.74244562698421E-4
7	0.0	0.0	0.0	1.74244562698421E-4	1.74244562698421E-4
8	0.0	0.0	0.0	1.74244562698421E-4	1.74244562698421E-4
9	0.0	0.0	0.0	1.74244562698421E-4	1.74244562698421E-4
10-11	0.0	0.0	0.0	2.613668440476315E-4	1.74244562698421E-4
12-13	0.0	0.0	0.0	5.22733688095263E-4	1.74244562698421E-4
14-15	0.0	0.0	0.0	5.22733688095263E-4	1.74244562698421E-4
16-17	0.0	0.0	0.0	5.22733688095263E-4	3.48489125396842E-4
18-19	0.0	0.0	0.0	6.098559694444736E-4	3.48489125396842E-4
20-21	0.0	0.0	0.0	7.841005321428945E-4	3.48489125396842E-4
22-23	0.0	0.0	0.0	0.0021780570337302622	3.48489125396842E-4
24-25	0.0	0.0	0.0	0.005750070569047893	3.48489125396842E-4
26-27	0.0	0.0	0.0	0.009844817792460787	3.48489125396842E-4
28-29	0.0	0.0	0.0	0.01864416820873105	3.48489125396842E-4
30-31	0.0	0.0	0.0	0.03685272501071604	3.48489125396842E-4
32-33	0.0	0.0	0.0	0.06516746644920945	3.48489125396842E-4
34-35	0.0	0.0	0.0	0.09618299860952839	3.48489125396842E-4
36-37	0.0	0.0	0.0	0.1486306119817531	3.48489125396842E-4
38-39	0.0	0.0	0.0	0.21710872512223256	3.48489125396842E-4
40-41	0.0	0.0	0.0	0.29011719689287097	3.48489125396842E-4
42-43	0.0	0.0	0.0	0.3575498426571599	3.48489125396842E-4
44-45	0.0	0.0	0.0	0.4271605454551791	3.48489125396842E-4
46-47	0.0	0.0	0.0	0.5155025387432786	4.356114067460525E-4
48-49	0.0	0.0	0.0	0.6043672657194732	5.22733688095263E-4
50-51	0.0	0.0	0.0	0.686610699313128	5.22733688095263E-4
52-53	0.0	0.0	0.0	0.7691154997508303	5.22733688095263E-4
54-55	0.0	0.0	0.0	0.8510975665004373	5.22733688095263E-4
56-57	0.0	0.0	0.0	0.9384812146936955	5.22733688095263E-4
58-59	0.0	0.0	0.0	1.0372778817437003	5.22733688095263E-4
60-61	0.0	0.0	0.0	1.138688217234181	5.22733688095263E-4
62-63	0.0	0.0	0.0	1.2355681940945034	5.22733688095263E-4
64-65	0.0	0.0	0.0	1.3163305489052215	5.22733688095263E-4
66-67	0.0	0.0	0.0	1.4153014605179246	5.22733688095263E-4
68-69	0.0	0.0	0.0	1.5242043122044375	5.22733688095263E-4
70-71	0.0	0.0	0.0	1.6510543538488882	5.22733688095263E-4
72-73	0.0	0.0	0.0	1.7688436782330208	5.22733688095263E-4
74-75	0.0	0.0	0.0	1.8749586169163592	5.22733688095263E-4
76-77	0.0	0.0	0.0	1.9856910365112057	5.22733688095263E-4
78-79	0.0	0.0	0.0	2.1146320129080376	5.22733688095263E-4
80-81	0.0	0.0	0.0	2.251501116907647	6.96978250793684E-4
82-83	0.0	0.0	0.0	2.3956884925405904	8.71222813492105E-4
84-85	0.0	0.0	0.0	2.5311636400386126	8.71222813492105E-4
86-87	0.0	0.0	0.0	2.670733534760048	8.71222813492105E-4
88-89	0.0	0.0	0.0	2.825375584154896	8.71222813492105E-4
90-91	0.0	0.0	0.0	2.988381372559269	8.71222813492105E-4
92-93	0.0	0.0	0.0	3.1403226312322925	8.71222813492105E-4
94-95	0.0	0.0	0.0	3.301498851728332	8.71222813492105E-4
96-97	0.0	0.0	0.0	3.4771373709283404	8.71222813492105E-4
98-99	0.0	0.0	0.0	3.6525145232843013	8.71222813492105E-4
100-101	0.0	0.0	0.0	3.8493237568521677	8.71222813492105E-4
102-103	0.0	0.0	0.0	4.06974312866567	8.71222813492105E-4
104-105	0.0	0.0	0.0	4.289814011353776	8.71222813492105E-4
106-107	0.0	0.0	0.0	4.515112230922834	8.71222813492105E-4
108-109	0.0	0.0	0.0	4.744069586308559	8.71222813492105E-4
110-111	0.0	0.0	0.0	4.973026941694284	9.583450948413156E-4
112-113	0.0	0.0	0.0	5.230908894487948	0.001045467376190526
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3020	0.0	39.586292	1
GTATCAA	4175	0.0	32.053963	1
TATCAAC	5185	0.0	26.151941	2
ATCAACG	5220	0.0	25.862661	3
CAACGCA	5245	0.0	25.626	5
TCAACGC	5330	0.0	25.440493	4
AACGCAG	5585	0.0	24.59839	6
GAGTACT	2755	0.0	22.666557	12-13
ACGCAGA	6055	0.0	22.296135	7
GTAAGTA	405	0.0	22.026937	2
CGCAGAG	6185	0.0	21.731346	8
TAGAACG	170	3.942504E-4	20.990374	7
CATGGGG	2695	0.0	20.964415	4
GCAGAGT	6555	0.0	20.50471	9
CAGAGTA	6505	0.0	19.702341	10-11
GTACTTT	3180	0.0	19.54371	14-15
AGTACTT	2880	0.0	19.51449	12-13
ATGGGAG	490	0.0	19.419666	5
TGTAAGT	430	4.9112714E-11	19.3649	1
TAACACT	215	8.582766E-5	19.363214	4
>>END_MODULE