FastQCFastQC Report
Sun 1 Jan 2017
ERR1142617_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142617_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887094
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21520.24258984955371132No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15410.17371327052150054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20600.044.254151
GTATCAA31350.038.5823141
CAACGCA38800.030.5460175
TATCAAC39500.030.4604742
ATCAACG39300.029.8576773
TCAACGC40350.029.228334
AACGCAG40900.028.6782936
ACGCAGA47050.024.8031487
CGCAGAG48150.024.4838268
GCAGAGT52700.022.0347489
GTACTAC2505.204256E-721.4502491
GTACATG65050.020.5177881
AGAGTAC48800.020.43991510-11
TACATGG66700.019.9141332
CAGAGTA51750.019.90764210-11
ACATGGG66550.019.601013
CATGGGG39300.019.3999124
GCGAATA800.00685428218.584244112-113
GAGTACA55000.018.08164212-13
AGTACAT54650.017.92502812-13
Produced by FastQC (version 0.11.5)