##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142617_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	887094
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193526277936723	33.0	33.0	33.0	27.0	33.0
2	31.26857807628053	33.0	33.0	33.0	27.0	33.0
3	31.375278155415323	33.0	33.0	33.0	27.0	33.0
4	31.32636563881618	33.0	33.0	33.0	27.0	33.0
5	31.33922898813429	33.0	33.0	33.0	27.0	33.0
6	34.54127071088295	37.0	37.0	37.0	27.0	37.0
7	34.654912557181085	37.0	37.0	37.0	27.0	37.0
8	34.685084106081206	37.0	37.0	37.0	27.0	37.0
9	34.57824537196735	37.0	37.0	37.0	27.0	37.0
10-11	34.630381335010725	37.0	37.0	37.0	27.0	37.0
12-13	34.638682597334665	37.0	37.0	37.0	27.0	37.0
14-15	34.601280698550546	37.0	37.0	37.0	27.0	37.0
16-17	34.59056819232235	37.0	37.0	37.0	27.0	37.0
18-19	34.60746493607216	37.0	37.0	37.0	27.0	37.0
20-21	34.6621987072396	37.0	37.0	37.0	27.0	37.0
22-23	34.708347706105556	37.0	37.0	37.0	27.0	37.0
24-25	34.76159065442896	37.0	37.0	37.0	27.0	37.0
26-27	34.733726076379725	37.0	37.0	37.0	27.0	37.0
28-29	34.7640638985271	37.0	37.0	37.0	27.0	37.0
30-31	34.823026082917934	37.0	37.0	37.0	27.0	37.0
32-33	34.83789654760375	37.0	37.0	37.0	27.0	37.0
34-35	34.831349327128805	37.0	37.0	37.0	27.0	37.0
36-37	34.78803880986682	37.0	37.0	37.0	27.0	37.0
38-39	34.76562010339378	37.0	37.0	37.0	27.0	37.0
40-41	34.7499915454281	37.0	37.0	37.0	27.0	37.0
42-43	34.74909817899794	37.0	37.0	37.0	27.0	37.0
44-45	34.76672314320692	37.0	37.0	37.0	27.0	37.0
46-47	34.75260400814345	37.0	37.0	37.0	27.0	37.0
48-49	34.734521933414044	37.0	37.0	37.0	27.0	37.0
50-51	34.712218772756884	37.0	37.0	37.0	27.0	37.0
52-53	34.67830748488886	37.0	37.0	37.0	27.0	37.0
54-55	34.635377987000254	37.0	37.0	37.0	27.0	37.0
56-57	34.568979724809324	37.0	37.0	37.0	27.0	37.0
58-59	34.519123114348645	37.0	37.0	37.0	27.0	37.0
60-61	34.48770367063693	37.0	37.0	37.0	27.0	37.0
62-63	34.53544832903841	37.0	37.0	37.0	27.0	37.0
64-65	34.47096361828622	37.0	37.0	37.0	27.0	37.0
66-67	34.44893269484406	37.0	37.0	37.0	27.0	37.0
68-69	34.41891614642867	37.0	37.0	37.0	27.0	37.0
70-71	34.3801085341576	37.0	37.0	37.0	27.0	37.0
72-73	34.34630490117169	37.0	37.0	37.0	27.0	37.0
74-75	34.27199146877332	37.0	37.0	37.0	27.0	37.0
76-77	34.216115203123906	37.0	37.0	37.0	27.0	37.0
78-79	34.144497088245444	37.0	37.0	37.0	27.0	37.0
80-81	34.06414483696203	37.0	37.0	37.0	27.0	37.0
82-83	34.03446083504116	37.0	37.0	37.0	27.0	37.0
84-85	34.008349735202806	37.0	37.0	37.0	27.0	37.0
86-87	33.93759680484819	37.0	37.0	37.0	27.0	37.0
88-89	33.88251977806185	37.0	37.0	37.0	27.0	37.0
90-91	33.80144381542429	37.0	35.0	37.0	22.0	37.0
92-93	33.66916471084237	37.0	33.0	37.0	22.0	37.0
94-95	33.59426622206891	37.0	33.0	37.0	22.0	37.0
96-97	33.50214746126115	37.0	33.0	37.0	22.0	37.0
98-99	33.44783247322155	37.0	33.0	37.0	22.0	37.0
100-101	33.312750396237604	37.0	33.0	37.0	22.0	37.0
102-103	33.24683235373027	37.0	33.0	37.0	18.0	37.0
104-105	33.123629513895935	37.0	33.0	37.0	14.0	37.0
106-107	33.02824052467946	37.0	33.0	37.0	14.0	37.0
108-109	32.89395937747297	37.0	33.0	37.0	14.0	37.0
110-111	32.804336969926524	37.0	33.0	37.0	14.0	37.0
112-113	32.7234075532018	37.0	33.0	37.0	14.0	37.0
114-115	32.54127972909297	37.0	33.0	37.0	14.0	37.0
116-117	32.35098478853425	37.0	33.0	37.0	14.0	37.0
118-119	32.245851059752404	37.0	33.0	37.0	14.0	37.0
120-121	32.1514349099419	37.0	33.0	37.0	14.0	37.0
122-123	31.950586972744716	37.0	33.0	37.0	14.0	37.0
124-125	30.27882783560705	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1560.0
1	114.0
2	46.0
3	38.0
4	33.0
5	33.0
6	29.0
7	26.0
8	27.0
9	21.0
10	25.0
11	26.0
12	19.0
13	25.0
14	75.0
15	305.0
16	496.0
17	827.0
18	1794.0
19	3265.0
20	4876.0
21	6175.0
22	7354.0
23	8529.0
24	9885.0
25	11384.0
26	13297.0
27	15567.0
28	18677.0
29	22268.0
30	26038.0
31	31637.0
32	39609.0
33	52330.0
34	77131.0
35	143615.0
36	389938.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.399942754288055	19.53074354939253	12.519501441890512	24.549812254428907
2	17.625773593397163	22.477828273290914	36.190318958508364	23.706079174803556
3	20.074871896920552	26.46340525799259	28.257979024110064	25.203743820976793
4	14.981377583902633	19.063949399492095	33.594635115974576	32.360037900630694
5	15.825680810639644	36.7034967882023	31.242762146023708	16.228060255134352
6	30.006967632181414	35.082819809792696	18.27250228484042	16.637710273185473
7	26.62436895608964	32.654903393582494	21.437677238077892	19.28305041224997
8	25.612539672844658	33.67379024687085	20.498673236014685	20.214996844269805
9	26.32759030283833	18.269172030725027	19.889362761179676	35.51387490525696
10-11	25.367573865435954	26.846183430614	25.68707298312294	22.09916972082711
12-13	26.081632606890686	25.648956940570162	25.23200465140481	23.037405801134344
14-15	24.529677338718844	25.96350754241175	23.524587985189466	25.982227133679935
16-17	24.248575154354747	27.77786256478434	24.211381920810066	23.76218036005085
18-19	24.25178994443217	27.071217377001556	25.484727748137708	23.192264930428575
20-21	25.963773922694642	25.74812636404427	25.08423026367096	23.203869449590123
22-23	25.998851738666573	25.546723838628598	24.75475127032061	23.699673152384225
24-25	24.941450336607595	26.10639794560602	24.922352834820735	24.02979888296565
26-27	24.38533910951369	26.26521572813153	25.060444785475394	24.289000376879383
28-29	24.35911560071525	26.22785682607209	25.20741349649579	24.205614076716863
30-31	24.78394031176324	25.821512691829422	25.322133314350246	24.07241368205709
32-33	24.390584252701608	26.19126174539111	25.050450381230878	24.36770362067641
34-35	24.706559042456416	26.153169004137045	25.161340262687148	23.978931690719392
36-37	24.605825093518906	26.30151025755906	24.87328831167657	24.219376337245468
38-39	24.539195301207066	26.194313253992057	25.03616563493637	24.230325809864507
40-41	24.281935946454613	26.146939056864856	25.31162016495062	24.259504831729913
42-43	24.361307992420773	26.219731970248045	25.137534447167454	24.281425590163728
44-45	24.434210221582287	26.219939637792866	25.080367144115197	24.265482996509657
46-47	24.52511274423681	26.119830048973057	24.99716126874622	24.357895938043914
48-49	24.349487317375175	26.34915291002183	25.088177344353713	24.213182428249286
50-51	24.131779687824807	26.528564843686453	25.106140921703137	24.23351454678561
52-53	24.17366194914799	26.271427320168733	25.095937320762076	24.458973409921196
54-55	24.024388314582364	26.03454276712787	25.30889379070934	24.632175127580425
56-57	24.210134974413677	26.10725745257759	24.88949884484412	24.793108728164615
58-59	24.18241532617791	26.136945962544473	25.189625179172843	24.49101353210478
60-61	24.264330682898905	26.23178520914058	25.256053890973735	24.247830216986777
62-63	23.953432136932932	26.152275511080376	25.361812815541	24.532479536445692
64-65	24.029702069618605	25.885968416407557	25.40524781011649	24.679081703857346
66-67	24.235633791277866	25.90677820059722	25.211583934367876	24.646004073757037
68-69	24.040881560192737	25.980219073730193	25.14014832022571	24.838751045851367
70-71	24.099823620004496	25.950344681086662	25.276575358098846	24.673256340809992
72-73	24.17110075830594	26.102628364551823	25.233870320757422	24.492400556384812
74-75	24.007644195789027	26.02924362456446	25.476547706741265	24.486564472905243
76-77	24.12672729440119	25.77042174824594	25.345176400267338	24.75767455708553
78-79	24.083140428720025	25.876884951632125	25.364475304160244	24.67549931548761
80-81	23.92199752676187	26.122546746064877	25.451473294056036	24.503982433117212
82-83	24.04301772760713	25.87109598809345	25.676269732656337	24.409616551643083
84-85	23.870348915045764	26.039579519155108	25.37255847873108	24.71751308706805
86-87	23.702364390279445	26.33313620196194	25.448869262361523	24.515630145397097
88-89	23.947895565168956	25.837780065009092	25.42607842893571	24.788245940886245
90-91	24.14872327554508	25.77708566680731	25.41316257246125	24.66102848518636
92-93	24.078624078624077	26.0431526239758	25.2992158947551	24.57900740264502
94-95	23.664937968526434	26.110779405426506	25.45456702240454	24.769715603642524
96-97	23.736680834239397	25.97404651294144	25.43413490729889	24.85513774552027
98-99	23.546949992827233	26.099494743548178	25.395321097083112	24.958234166541473
100-101	23.818400725556792	26.33339695885086	25.27414503681433	24.574057278778028
102-103	23.84172424582318	26.207467951650454	25.51793001943967	24.432877783086695
104-105	23.81685502594823	26.31990335703285	25.478400195183553	24.384841421835368
106-107	23.84044657879146	26.196179072040987	25.45570272961346	24.50767161955409
108-109	23.83419601331718	26.11505976635633	25.328510232147046	24.722233988179447
110-111	23.49861975801715	26.6679806449864	25.272434014945468	24.56096558205098
112-113	23.619863862749074	26.689192432652835	25.18951405450799	24.501429650090106
114-115	23.489566009468042	26.69766585592154	25.250748390980572	24.562019743629854
116-117	23.517794039492358	26.7503887054316	25.182464231475848	24.549353023600197
118-119	23.421961897265252	26.435713281410138	25.414904664109468	24.727420157215143
120-121	23.487241633622048	26.486894905124014	25.497024572547012	24.52883888870693
122-123	23.49643612641784	26.695112902114605	25.41503230695732	24.393418664510232
124-125	23.476624790506264	26.57916491617992	25.19656272163421	24.74764757167961
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2657.0
1	1638.5
2	513.5
3	354.0
4	253.5
5	203.0
6	187.0
7	174.0
8	176.5
9	183.5
10	168.5
11	138.0
12	147.5
13	174.0
14	182.0
15	211.5
16	246.0
17	278.5
18	293.5
19	319.0
20	407.0
21	518.0
22	643.5
23	822.0
24	1083.0
25	1393.0
26	1812.0
27	2427.0
28	3176.5
29	4235.5
30	5623.0
31	6985.5
32	8561.0
33	10154.0
34	11531.5
35	13128.0
36	14823.5
37	16691.0
38	18543.0
39	19965.0
40	20916.5
41	21702.5
42	22809.0
43	24692.5
44	27074.0
45	29168.5
46	31386.0
47	33746.5
48	35799.0
49	37168.0
50	37364.5
51	37138.0
52	37013.5
53	37145.5
54	36872.5
55	35787.0
56	33895.5
57	30494.5
58	27294.0
59	24401.5
60	21084.5
61	17919.5
62	14857.5
63	11902.5
64	9313.0
65	7381.0
66	5820.5
67	4607.0
68	3881.0
69	3306.0
70	2754.0
71	2321.0
72	1996.5
73	1784.5
74	1503.5
75	1308.0
76	1062.5
77	773.5
78	582.5
79	412.5
80	301.0
81	214.5
82	153.5
83	113.5
84	73.5
85	41.5
86	18.0
87	7.0
88	6.0
89	5.5
90	4.0
91	3.5
92	2.0
93	1.0
94	2.5
95	1.5
96	0.0
97	2.0
98	2.5
99	1.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35903748644450306
2	0.3096627865818053
3	0.29872820693184715
4	0.301659125188537
5	0.32172464248433646
6	0.3388592415234462
7	0.34303016365796635
8	0.3368301442688148
9	0.35216110130380773
10-11	0.34427016753579665
12-13	0.3449465332873405
14-15	0.33745014620772995
16-17	0.2845808899620559
18-19	0.28176269933062337
20-21	0.26118990772116596
22-23	0.2567935303361312
24-25	0.2436607619936557
26-27	0.24749350125240394
28-29	0.23520619009935814
30-31	0.23622073872667385
32-33	0.23289527378158345
34-35	0.24382985343154162
36-37	0.23813710835604796
38-39	0.21762068055921918
40-41	0.243773489618913
42-43	0.23030253840066553
44-45	0.21840977393602032
46-47	0.22720252870608976
48-49	0.2193679587507074
50-51	0.21981886925173658
52-53	0.2560608007719588
54-55	0.24371712580628432
56-57	0.24219530286531077
58-59	0.23948983985913555
60-61	0.2559480731467015
62-63	0.2374043787918755
64-65	0.24490076587148601
66-67	0.2436607619936557
68-69	0.231035267964838
70-71	0.2336843671583846
72-73	0.2361643749140452
74-75	0.22297524275894098
76-77	0.23396618622152782
78-79	0.2432098514926265
80-81	0.227653439207119
82-83	0.24833895844183365
84-85	0.22145341981796743
86-87	0.2139006689257283
88-89	0.22398979138625671
90-91	0.21187157167109685
92-93	0.23362800334575592
94-95	0.21852250156127762
96-97	0.20927883629017893
98-99	0.20302245308839875
100-101	0.19220060106369788
102-103	0.20189517683582575
104-105	0.19992244339382298
106-107	0.2012751748969106
108-109	0.19969698814330836
110-111	0.196371523198218
112-113	0.19586424888456017
114-115	0.19101696099849622
116-117	0.20104971964639595
118-119	0.18256238910419864
120-121	0.18881877230597885
122-123	0.18949513805752266
124-125	0.1832951186683711
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	887094.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.46079316520017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.221822086901	70.9839777959123
2	8.236011889330934	13.253520982671715
3	1.781369809522724	4.299912833842198
4	0.6564086164542808	2.1126063168153237
5	0.34642915843514116	1.3936982431622125
6	0.20027946923733567	0.9668786969724801
7	0.1285365759911575	0.723950839849129
8	0.09019365240412452	0.5805642248721778
9	0.059196155898102995	0.42866726903029884
>10	0.26458607720846294	3.550712911614252
>50	0.009372808165853652	0.5204891750969578
>100	0.0054528945450613075	0.6786824635026648
>500	0.0	0.0
>1k	3.4080590576886715E-4	0.5063382466583549
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2152	0.24258984955371132	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1541	0.17371327052150054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.127276252573008E-4	0.0
5	0.0	0.0	0.0	1.127276252573008E-4	0.0
6	0.0	0.0	0.0	1.127276252573008E-4	0.0
7	0.0	0.0	0.0	1.127276252573008E-4	0.0
8	0.0	0.0	0.0	1.127276252573008E-4	1.127276252573008E-4
9	0.0	0.0	0.0	1.127276252573008E-4	1.127276252573008E-4
10-11	0.0	0.0	0.0	1.127276252573008E-4	1.127276252573008E-4
12-13	0.0	0.0	0.0	1.127276252573008E-4	1.127276252573008E-4
14-15	0.0	0.0	0.0	2.254552505146016E-4	1.127276252573008E-4
16-17	0.0	0.0	0.0	3.945466884005528E-4	1.127276252573008E-4
18-19	0.0	0.0	0.0	5.072743136578536E-4	1.127276252573008E-4
20-21	0.0	0.0	0.0	0.001127276252573008	4.509105010292032E-4
22-23	0.0	0.0	0.0	0.0021981886925173657	4.509105010292032E-4
24-25	0.0	0.0	0.0	0.004565468822920683	4.509105010292032E-4
26-27	0.0	0.0	0.0	0.007947297580639707	4.509105010292032E-4
28-29	0.0	0.0	0.0	0.013865497906648	4.509105010292032E-4
30-31	0.0	0.0	0.0	0.03032373119421392	4.509105010292032E-4
32-33	0.0	0.0	0.0	0.057716544131738004	4.509105010292032E-4
34-35	0.0	0.0	0.0	0.08837845820172383	4.509105010292032E-4
36-37	5.63638126286504E-5	0.0	0.0	0.13482223980773175	4.509105010292032E-4
38-39	1.127276252573008E-4	0.0	0.0	0.19834425664022076	4.509105010292032E-4
40-41	1.127276252573008E-4	0.0	0.0	0.2627680944747682	4.509105010292032E-4
42-43	1.127276252573008E-4	0.0	0.0	0.31721553747404446	4.509105010292032E-4
44-45	1.127276252573008E-4	0.0	0.0	0.38090664574441946	4.509105010292032E-4
46-47	1.127276252573008E-4	0.0	0.0	0.46545236468739504	5.072743136578536E-4
48-49	1.127276252573008E-4	0.0	0.0	0.5502799026935139	5.63638126286504E-4
50-51	1.127276252573008E-4	0.0	0.0	0.6210728513550988	5.63638126286504E-4
52-53	1.127276252573008E-4	0.0	0.0	0.6892730646357658	5.63638126286504E-4
54-55	1.127276252573008E-4	0.0	0.0	0.7597841942342074	5.63638126286504E-4
56-57	1.127276252573008E-4	0.0	0.0	0.8368335260975726	5.63638126286504E-4
58-59	1.127276252573008E-4	0.0	0.0	0.9245356185477525	5.63638126286504E-4
60-61	1.127276252573008E-4	0.0	0.0	1.0153377206925083	5.63638126286504E-4
62-63	1.127276252573008E-4	0.0	0.0	1.0980234338187385	5.63638126286504E-4
64-65	1.127276252573008E-4	0.0	0.0	1.1813291488838837	6.200019389151544E-4
66-67	1.127276252573008E-4	0.0	0.0	1.2661566868900027	6.763657515438048E-4
68-69	1.127276252573008E-4	0.0	0.0	1.3669915476826582	6.763657515438048E-4
70-71	1.127276252573008E-4	0.0	0.0	1.4721100582350912	6.763657515438048E-4
72-73	1.127276252573008E-4	0.0	0.0	1.5761576563475799	6.763657515438048E-4
74-75	1.127276252573008E-4	0.0	0.0	1.6762034237634342	7.890933768011057E-4
76-77	1.127276252573008E-4	0.0	0.0	1.781378298128496	7.890933768011057E-4
78-79	1.127276252573008E-4	0.0	0.0	1.9032368610316381	7.890933768011057E-4
80-81	1.127276252573008E-4	0.0	0.0	2.038340919902513	7.890933768011057E-4
82-83	1.127276252573008E-4	0.0	0.0	2.1657231364432628	7.890933768011057E-4
84-85	1.127276252573008E-4	0.0	0.0	2.2932180806092703	7.890933768011057E-4
86-87	1.127276252573008E-4	0.0	0.0	2.420431205712134	7.890933768011057E-4
88-89	1.127276252573008E-4	0.0	0.0	2.56010073340593	7.890933768011057E-4
90-91	1.127276252573008E-4	0.0	0.0	2.7077175586803657	7.890933768011057E-4
92-93	1.127276252573008E-4	0.0	0.0	2.859505306089321	9.018210020584064E-4
94-95	1.127276252573008E-4	0.0	0.0	3.011067598247762	9.018210020584064E-4
96-97	1.127276252573008E-4	0.0	0.0	3.1624607989683167	9.018210020584064E-4
98-99	1.127276252573008E-4	0.0	0.0	3.323661303086257	9.018210020584064E-4
100-101	1.127276252573008E-4	0.0	0.0	3.495063657289983	9.581848146870569E-4
102-103	1.127276252573008E-4	0.0	0.0	3.687433349791567	0.0010145486273157073
104-105	1.127276252573008E-4	0.0	0.0	3.87523757347023	0.0010145486273157073
106-107	1.127276252573008E-4	0.0	0.0	4.0735818301104505	0.0010145486273157073
108-109	1.127276252573008E-4	0.0	0.0	4.2709115381233556	0.0010709124399443577
110-111	1.127276252573008E-4	0.0	0.0	4.479401281036734	0.0011836400652016584
112-113	1.127276252573008E-4	0.0	0.0	4.705251078239735	0.0012400038778303088
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2060	0.0	44.25415	1
GTATCAA	3135	0.0	38.582314	1
CAACGCA	3880	0.0	30.546017	5
TATCAAC	3950	0.0	30.460474	2
ATCAACG	3930	0.0	29.857677	3
TCAACGC	4035	0.0	29.22833	4
AACGCAG	4090	0.0	28.678293	6
ACGCAGA	4705	0.0	24.803148	7
CGCAGAG	4815	0.0	24.483826	8
GCAGAGT	5270	0.0	22.034748	9
GTACTAC	250	5.204256E-7	21.450249	1
GTACATG	6505	0.0	20.517788	1
AGAGTAC	4880	0.0	20.439915	10-11
TACATGG	6670	0.0	19.914133	2
CAGAGTA	5175	0.0	19.907642	10-11
ACATGGG	6655	0.0	19.60101	3
CATGGGG	3930	0.0	19.399912	4
GCGAATA	80	0.006854282	18.584244	112-113
GAGTACA	5500	0.0	18.081642	12-13
AGTACAT	5465	0.0	17.925028	12-13
>>END_MODULE