FastQCFastQC Report
Sun 1 Jan 2017
ERR1142617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887094
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36320.40942673493451653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34910.3935321397732371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.2661499232324872No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.12411311540828818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27400.056.663691
GTATCAA43000.043.715311
TATCAAC56150.033.4717752
ATCAACG57550.032.7608643
CAACGCA57450.032.6108365
TCAACGC58100.032.2460024
AACGCAG60800.030.9101056
ACGCAGA69300.027.0330037
CGCAGAG71250.026.2096828
GTGGTAT14150.026.0645451
GCAGAGT76400.024.5207739
TGGTATC15750.023.412762
GAGTACT33950.023.38613312-13
CAGAGTA75000.022.79783210-11
AGAGTAC72950.022.2563710-11
GTACTTT37400.021.22885914-15
AGTACTT35550.020.3260812-13
GTACATG60150.018.097991
ACTTTTT44600.018.06847216-17
AGTACAT52000.017.61304312-13
Produced by FastQC (version 0.11.5)