##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	887094
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.083389133507836	33.0	33.0	33.0	14.0	33.0
2	29.26068263340751	33.0	27.0	33.0	14.0	33.0
3	30.80720194252244	33.0	33.0	33.0	27.0	33.0
4	31.535375056081993	33.0	33.0	33.0	27.0	33.0
5	31.861067710975387	33.0	33.0	33.0	33.0	33.0
6	35.048689315901136	37.0	37.0	37.0	33.0	37.0
7	35.84113633955364	37.0	37.0	37.0	33.0	37.0
8	35.994377146052166	37.0	37.0	37.0	33.0	37.0
9	36.05757112549516	37.0	37.0	37.0	37.0	37.0
10-11	36.09837288945704	37.0	37.0	37.0	37.0	37.0
12-13	36.17283568595887	37.0	37.0	37.0	37.0	37.0
14-15	36.16685548543897	37.0	37.0	37.0	37.0	37.0
16-17	36.2095944736409	37.0	37.0	37.0	37.0	37.0
18-19	36.21361828622446	37.0	37.0	37.0	37.0	37.0
20-21	36.22178822086498	37.0	37.0	37.0	37.0	37.0
22-23	36.218187700514264	37.0	37.0	37.0	37.0	37.0
24-25	36.21155086157724	37.0	37.0	37.0	37.0	37.0
26-27	36.161819378780606	37.0	37.0	37.0	37.0	37.0
28-29	36.14466899787396	37.0	37.0	37.0	37.0	37.0
30-31	36.124913481547615	37.0	37.0	37.0	37.0	37.0
32-33	36.073185028869545	37.0	37.0	37.0	37.0	37.0
34-35	35.97748829323612	37.0	37.0	37.0	37.0	37.0
36-37	35.900385415750755	37.0	37.0	37.0	35.0	37.0
38-39	35.89168678854777	37.0	37.0	37.0	33.0	37.0
40-41	35.79601316207753	37.0	37.0	37.0	33.0	37.0
42-43	35.76318011394508	37.0	37.0	37.0	33.0	37.0
44-45	35.68490374188079	37.0	37.0	37.0	33.0	37.0
46-47	35.57413475911234	37.0	37.0	37.0	33.0	37.0
48-49	35.445177737646745	37.0	37.0	37.0	33.0	37.0
50-51	35.36399186557456	37.0	37.0	37.0	33.0	37.0
52-53	35.280502404480245	37.0	37.0	37.0	33.0	37.0
54-55	35.23112770461755	37.0	37.0	37.0	33.0	37.0
56-57	35.227063310088894	37.0	37.0	37.0	33.0	37.0
58-59	35.19016868561843	37.0	37.0	37.0	33.0	37.0
60-61	35.15939404392319	37.0	37.0	37.0	33.0	37.0
62-63	35.146180111690526	37.0	37.0	37.0	33.0	37.0
64-65	35.10847835742322	37.0	37.0	37.0	33.0	37.0
66-67	35.08939977048655	37.0	37.0	37.0	33.0	37.0
68-69	35.06814441310617	37.0	37.0	37.0	33.0	37.0
70-71	34.95624702680888	37.0	37.0	37.0	27.0	37.0
72-73	34.96155255249162	37.0	37.0	37.0	27.0	37.0
74-75	34.8947772163942	37.0	37.0	37.0	27.0	37.0
76-77	34.79194369480574	37.0	37.0	37.0	27.0	37.0
78-79	34.79165962119009	37.0	37.0	37.0	27.0	37.0
80-81	34.77916883667345	37.0	37.0	37.0	27.0	37.0
82-83	34.69908036803315	37.0	37.0	37.0	27.0	37.0
84-85	34.64918373926551	37.0	37.0	37.0	27.0	37.0
86-87	34.53347615923454	37.0	37.0	37.0	27.0	37.0
88-89	34.45379632823579	37.0	37.0	37.0	27.0	37.0
90-91	34.39735586082196	37.0	37.0	37.0	27.0	37.0
92-93	34.23219805341937	37.0	37.0	37.0	27.0	37.0
94-95	34.28306075793546	37.0	37.0	37.0	27.0	37.0
96-97	34.28008193043804	37.0	37.0	37.0	27.0	37.0
98-99	34.25184366031108	37.0	37.0	37.0	27.0	37.0
100-101	34.13183833956717	37.0	37.0	37.0	27.0	37.0
102-103	34.06701770049172	37.0	37.0	37.0	24.5	37.0
104-105	33.92650891562788	37.0	37.0	37.0	22.0	37.0
106-107	33.90096145391582	37.0	37.0	37.0	22.0	37.0
108-109	33.79065690896343	37.0	33.0	37.0	22.0	37.0
110-111	33.72403600971261	37.0	33.0	37.0	22.0	37.0
112-113	33.58598525071751	37.0	33.0	37.0	22.0	37.0
114-115	33.56199963025339	37.0	33.0	37.0	22.0	37.0
116-117	33.42144293614882	37.0	33.0	37.0	22.0	37.0
118-119	33.3044029155873	37.0	33.0	37.0	22.0	37.0
120-121	33.214056796686705	37.0	33.0	37.0	18.0	37.0
122-123	33.14543216389695	37.0	33.0	37.0	14.0	37.0
124-125	31.748319231107413	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	90.0
16	34.0
17	14.0
18	76.0
19	360.0
20	1357.0
21	3385.0
22	5857.0
23	8707.0
24	8250.0
25	6347.0
26	6202.0
27	7344.0
28	9595.0
29	12423.0
30	16597.0
31	22525.0
32	32415.0
33	48302.0
34	78567.0
35	161775.0
36	456870.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.19268624623256	19.841308321221547	12.727356781154544	24.238648651391344
2	17.769255569308324	23.01413378965476	34.96202206305081	24.254588577986098
3	21.73032785776447	25.232557615219164	28.56682283235561	24.470291694660755
4	16.282881572791787	17.880586415056055	33.42622183894441	32.410310173207755
5	16.650264187017576	35.587856979396	31.348238734427508	16.413640099158908
6	30.681362138080615	34.04612457106268	18.338902191018583	16.93361109983812
7	26.574635833406607	31.479302080726505	22.396386403244755	19.549675682622134
8	26.227126129954808	31.960572341344147	21.023725810033447	20.788575718667605
9	26.565955806261794	16.209443418623053	20.825526945284263	36.39907382983088
10-11	25.860168144525836	25.082685713126228	26.445562702486995	22.611583439860937
12-13	26.81291589495126	23.87872522948552	26.4439579615666	22.864400913996615
14-15	24.825610363726955	24.41409816772518	25.143840449828314	25.616451018719548
16-17	23.997456864774193	26.23076021255921	25.97723578335554	23.794547139311053
18-19	23.789474396174477	25.022658252676717	28.18788087846384	22.99998647268497
20-21	25.013851492833016	23.97556726301829	28.169342743142312	22.841238501006384
22-23	25.003550920195604	23.811118100223876	27.993763907770763	23.19156707180975
24-25	24.128559092948436	24.407052691146596	28.093978766624506	23.37040944928046
26-27	23.73288512829531	24.643273429873272	28.145326199929205	23.478515241902212
28-29	23.61059991601683	24.53447114558116	28.39764058021661	23.457288358185398
30-31	23.96148090531709	24.296395246268006	28.423529677006627	23.31859417140828
32-33	23.75470850526693	24.56482786724932	28.05877409489326	23.62168953259049
34-35	23.90688021787995	24.61836062469141	28.127515235138556	23.347243922290083
36-37	23.979972584332486	24.622132740529644	27.944873315266648	23.45302135987122
38-39	23.87999467925609	24.562560450189043	27.945065573659612	23.612379296895256
40-41	23.69445563919522	24.652663677433356	28.12752058033588	23.52536010303555
42-43	23.85998355280772	24.60873099033178	27.897042154525824	23.634243302334678
44-45	23.89645291254365	24.765526539464812	27.6294845867518	23.708535961239733
46-47	23.91668752127734	24.746757389859475	27.417894834143848	23.91866025471934
48-49	23.918547527094084	25.03505829145502	27.281325316144624	23.76506886530627
50-51	23.769972517004962	25.14902592059015	27.283805323900285	23.7971962385046
52-53	23.803621713144267	25.013809134094018	27.14419216001912	24.038376992742595
54-55	23.792123495368024	24.90728152822587	27.18731047668004	24.113284499726074
56-57	23.8357490863426	24.873970514962338	26.946580632943068	24.343699765751996
58-59	23.78214710053275	25.002141824879885	27.040426380969773	24.175284693617588
60-61	23.818839942554	24.966350803860696	27.366040126525487	23.848769127059814
62-63	23.555677301390833	25.01854369435483	27.334250936202924	24.091528068051414
64-65	23.54711000187128	24.656011651527347	27.474991376336668	24.321886970264707
66-67	23.740212423937034	24.74861739567622	27.323992722304514	24.187177458082232
68-69	23.63002117024802	24.774150202797	27.358769194696393	24.237059432258587
70-71	23.568077340169136	24.88214326779349	27.45520767810401	24.09457171393336
72-73	23.6451266720325	24.93619616410437	27.44263854788782	23.97603861597531
74-75	23.564933043368406	24.815181198000325	27.617636575285605	24.00224918334567
76-77	23.54885619938018	24.740829908380878	27.482634560299285	24.22767933193966
78-79	23.530101392919686	24.889371864408883	27.494846935525963	24.085679807145468
80-81	23.435115106178152	25.076542057549705	27.52492971432565	23.963413121946488
82-83	23.499595307825327	24.94696165231644	27.763686824620613	23.789756215237617
84-85	23.481209843343922	25.010828761189718	27.38977223505631	24.11818916041005
86-87	23.212452312400554	25.178318743660704	27.492158603635968	24.117070340302774
88-89	23.38134106606187	24.86956384271892	27.436450307225773	24.312644783993434
90-91	23.615093913926337	24.822550623272605	27.336849230075018	24.225506232726044
92-93	23.588387255504983	24.986146551986828	27.20817189787159	24.2172942946366
94-95	23.22168350639	25.01468285594794	27.349566954386763	24.414066683275298
96-97	23.282932812080794	24.982076307584087	27.220677853756197	24.514313026578918
98-99	23.19585072156953	25.088829368702754	27.061450083080256	24.65386982664746
100-101	23.37587673910544	25.249635326132292	26.862880371189522	24.511607563572742
102-103	23.50404804902299	25.17872964984545	27.026109972562097	24.291112328569465
104-105	23.390131819608865	25.28668383528721	26.86945479202352	24.453729553080404
106-107	23.430944184043632	25.18616967311243	26.837234836443493	24.545651306400448
108-109	23.448171297066082	25.153126992116366	26.723147187350104	24.675554523467447
110-111	23.238487960112412	25.5912288790465	26.617840519539666	24.55244264130142
112-113	23.296585985581	25.541388816398726	26.501603269553886	24.66042192846639
114-115	23.190157976494035	25.602416429375012	26.612625043118314	24.594800551012632
116-117	23.279374133402474	25.550282383974572	26.537329921428494	24.633013561194467
118-119	23.14247000311129	25.44521875972278	26.67874358014727	24.73356765701866
120-121	23.11510743518436	25.335167789622957	26.865503391414432	24.68422138377825
122-123	23.12150685271234	25.59503276992066	26.745700004734562	24.537760372632437
124-125	23.098935611364	25.436205446621617	26.67346423442252	24.791394707591856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	400.0
1	352.0
2	460.5
3	660.5
4	525.5
5	428.0
6	741.0
7	1492.5
8	2155.0
9	2452.5
10	2272.5
11	1763.0
12	1569.0
13	1504.5
14	1406.0
15	1335.5
16	1288.5
17	1252.0
18	1205.5
19	1156.0
20	1142.0
21	1150.5
22	1225.5
23	1357.5
24	1472.5
25	1665.5
26	1988.5
27	2435.0
28	2995.0
29	3900.5
30	5258.5
31	6428.0
32	7905.5
33	9445.0
34	10711.0
35	12534.0
36	14145.5
37	15742.0
38	17633.5
39	19047.0
40	19955.5
41	20842.0
42	22033.0
43	23932.5
44	26114.5
45	28053.5
46	30653.0
47	33072.0
48	35110.0
49	36634.5
50	36571.5
51	36293.5
52	36264.5
53	36479.0
54	36438.0
55	35575.5
56	33914.0
57	30742.5
58	27617.5
59	24498.0
60	21064.0
61	17875.5
62	14694.0
63	11792.0
64	9209.5
65	7142.5
66	5569.5
67	4476.0
68	3724.5
69	3131.0
70	2654.0
71	2299.0
72	2019.0
73	1818.5
74	1495.0
75	1276.0
76	1078.5
77	781.5
78	585.0
79	390.0
80	260.0
81	199.0
82	139.0
83	97.5
84	61.0
85	34.5
86	15.0
87	8.0
88	6.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024461894680834276
2	0.0
3	2.254552505146016E-4
4	0.0010145486273157073
5	0.0037200116334909264
6	0.0020290972546314146
7	0.0
8	1.127276252573008E-4
9	0.0
10-11	0.0
12-13	1.127276252573008E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.200019389151544E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0012963676904589592
30-31	1.6909143788595122E-4
32-33	2.81819063143252E-4
34-35	0.0
36-37	0.001127276252573008
38-39	0.0
40-41	0.0024236439430319673
42-43	0.00140909531571626
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.06200019389151544
76-77	0.07969843105691167
78-79	5.63638126286504E-5
80-81	0.0
82-83	0.0
84-85	0.06380383589563225
86-87	0.14096589538425466
88-89	0.16993689507538096
90-91	0.07259659066570172
92-93	0.11644763689079173
94-95	5.63638126286504E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0011836400652016584
106-107	0.0
108-109	3.9454668840055283E-4
110-111	1.127276252573008E-4
112-113	5.63638126286504E-5
114-115	0.0
116-117	4.509105010292032E-4
118-119	2.254552505146016E-4
120-121	1.127276252573008E-4
122-123	0.0
124-125	0.0013527315030876098
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	887094.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.86622630321801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.67202690594068	66.62151634310636
2	9.273693096731703	14.256675844799396
3	2.01718154300656	4.651593989382502
4	0.7877047100836343	2.421915540215975
5	0.36461894858863003	1.4013441308327523
6	0.22373026189831663	1.0318380565172545
7	0.14795986179961237	0.7961181354617307
8	0.09860067004321259	0.6063249133752405
9	0.07658201535990691	0.5297913471070009
>10	0.3136363445751744	4.075013616347511
>50	0.014274199979754943	0.7641945060480554
>100	0.008564093144395324	1.0396860112105566
>500	7.136744242628807E-4	0.35232203557968994
>1k	7.136744242628807E-4	1.4516655300159667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3632	0.40942673493451653	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3491	0.3935321397732371	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2361	0.2661499232324872	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1101	0.12411311540828818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.127276252573008E-4	0.0
5	0.0	0.0	0.0	1.127276252573008E-4	0.0
6	0.0	0.0	0.0	1.127276252573008E-4	0.0
7	0.0	0.0	0.0	1.127276252573008E-4	0.0
8	0.0	0.0	0.0	1.127276252573008E-4	0.0
9	0.0	0.0	0.0	1.127276252573008E-4	0.0
10-11	0.0	0.0	0.0	1.127276252573008E-4	0.0
12-13	0.0	0.0	0.0	1.127276252573008E-4	0.0
14-15	0.0	0.0	0.0	2.254552505146016E-4	3.381828757719024E-4
16-17	0.0	0.0	0.0	3.945466884005528E-4	3.381828757719024E-4
18-19	0.0	0.0	0.0	5.072743136578536E-4	3.381828757719024E-4
20-21	0.0	0.0	0.0	0.001127276252573008	3.381828757719024E-4
22-23	0.0	0.0	0.0	0.0021981886925173657	3.381828757719024E-4
24-25	0.0	0.0	0.0	0.004678196448177983	3.381828757719024E-4
26-27	0.0	0.0	0.0	0.008172752831154309	3.381828757719024E-4
28-29	0.0	0.0	0.0	0.01392186171927665	3.381828757719024E-4
30-31	0.0	0.0	0.0	0.03071827788261447	3.381828757719024E-4
32-33	0.0	0.0	0.0	0.05799836319488127	3.381828757719024E-4
34-35	0.0	0.0	0.0	0.08854754963960978	3.381828757719024E-4
36-37	0.0	0.0	0.0	0.13566769699716152	3.381828757719024E-4
38-39	0.0	0.0	0.0	0.1994151690801651	3.381828757719024E-4
40-41	0.0	0.0	0.0	0.26474082791677095	3.381828757719024E-4
42-43	0.0	0.0	0.0	0.32054100241913486	3.381828757719024E-4
44-45	0.0	0.0	0.0	0.3844575659400244	3.945466884005528E-4
46-47	0.0	0.0	0.0	0.4686651020072281	4.509105010292032E-4
48-49	0.0	0.0	0.0	0.5538308228891189	6.763657515438048E-4
50-51	0.0	0.0	0.0	0.6244546801128178	6.763657515438048E-4
52-53	0.0	0.0	0.0	0.6929930762692567	6.763657515438048E-4
54-55	0.0	0.0	0.0	0.7646314821202713	6.763657515438048E-4
56-57	0.0	0.0	0.0	0.8428644540488381	6.763657515438048E-4
58-59	0.0	0.0	0.0	0.9325956437536496	6.763657515438048E-4
60-61	0.0	0.0	0.0	1.0240177478373205	6.763657515438048E-4
62-63	0.0	0.0	0.0	1.1065907333382934	6.763657515438048E-4
64-65	0.0	0.0	0.0	1.190009176028696	6.763657515438048E-4
66-67	0.0	0.0	0.0	1.2746112587843001	6.763657515438048E-4
68-69	0.0	0.0	0.0	1.374600662387526	6.763657515438048E-4
70-71	0.0	0.0	0.0	1.4794373538768157	6.763657515438048E-4
72-73	0.0	0.0	0.0	1.583879498677705	6.763657515438048E-4
74-75	0.0	0.0	0.0	1.6842634489693313	6.763657515438048E-4
76-77	0.0	0.0	0.0	1.7905092357743373	6.763657515438048E-4
78-79	0.0	0.0	0.0	1.9111277947996492	6.763657515438048E-4
80-81	0.0	0.0	0.0	2.0464009451084104	7.327295641724552E-4
82-83	0.0	0.0	0.0	2.1745158912133324	7.890933768011057E-4
84-85	0.0	0.0	0.0	2.30201083537934	7.890933768011057E-4
86-87	0.0	0.0	0.0	2.4299003262337475	7.890933768011057E-4
88-89	0.0	0.0	0.0	2.56844257767497	7.890933768011057E-4
90-91	0.0	0.0	0.0	2.7149884905094614	7.890933768011057E-4
92-93	0.0	0.0	0.0	2.866268963604759	7.890933768011057E-4
94-95	0.0	0.0	0.0	3.0189021682031445	7.890933768011057E-4
96-97	0.0	0.0	0.0	3.172155374740445	7.890933768011057E-4
98-99	0.0	0.0	0.0	3.3339195169846714	7.890933768011057E-4
100-101	0.0	0.0	0.0	3.506843694129371	7.890933768011057E-4
102-103	0.0	0.0	0.0	3.6992133866309542	9.018210020584064E-4
104-105	0.0	0.0	0.0	3.8879194313116763	9.018210020584064E-4
106-107	0.0	0.0	0.0	4.087278236579213	9.018210020584064E-4
108-109	0.0	0.0	0.0	4.286355222783605	9.018210020584064E-4
110-111	0.0	0.0	0.0	4.49540860382327	0.0010145486273157073
112-113	0.0	0.0	0.0	4.722780223967247	0.0010145486273157073
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2740	0.0	56.66369	1
GTATCAA	4300	0.0	43.71531	1
TATCAAC	5615	0.0	33.471775	2
ATCAACG	5755	0.0	32.760864	3
CAACGCA	5745	0.0	32.610836	5
TCAACGC	5810	0.0	32.246002	4
AACGCAG	6080	0.0	30.910105	6
ACGCAGA	6930	0.0	27.033003	7
CGCAGAG	7125	0.0	26.209682	8
GTGGTAT	1415	0.0	26.064545	1
GCAGAGT	7640	0.0	24.520773	9
TGGTATC	1575	0.0	23.41276	2
GAGTACT	3395	0.0	23.386133	12-13
CAGAGTA	7500	0.0	22.797832	10-11
AGAGTAC	7295	0.0	22.25637	10-11
GTACTTT	3740	0.0	21.228859	14-15
AGTACTT	3555	0.0	20.32608	12-13
GTACATG	6015	0.0	18.09799	1
ACTTTTT	4460	0.0	18.068472	16-17
AGTACAT	5200	0.0	17.613043	12-13
>>END_MODULE