FastQCFastQC Report
Sun 1 Jan 2017
ERR1142603_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142603_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535147
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18270.3414015214511153No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8830.16500139214085102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.033.608821
GTATCAA30100.026.1204432
CATGGGG25550.023.7783854
GTACATA2153.3773995E-622.1690331
CAACGCA36150.021.5740956
ATCAACG35600.021.415744
ATGGGGA8400.021.266355
TATCAAC36500.021.214053
AACGCAG37050.021.2127027
TCAACGC36550.021.0161575
ATGGGGG14500.020.9436875
GTACATG54400.020.5898971
GTCTAGA1450.003835006820.5445791
TACATGG54800.020.1077882
ACATGGG54800.019.3469523
ACGCAGA40700.019.3103358
TCGCATG1550.005649866519.2137172
CATGGGT8400.019.1450984
CGCAGAG41700.018.7044759
TCTACAC2551.4805613E-518.6862833
Produced by FastQC (version 0.11.5)