##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142603_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	535147
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227300162385287	33.0	33.0	33.0	27.0	33.0
2	31.303673570065794	33.0	33.0	33.0	27.0	33.0
3	31.420938545857492	33.0	33.0	33.0	27.0	33.0
4	31.345277092088715	33.0	33.0	33.0	27.0	33.0
5	31.37154090371431	33.0	33.0	33.0	27.0	33.0
6	34.583940487380104	37.0	37.0	37.0	27.0	37.0
7	34.71060661836841	37.0	37.0	37.0	27.0	37.0
8	34.71631533018031	37.0	37.0	37.0	27.0	37.0
9	34.60358368822025	37.0	37.0	37.0	27.0	37.0
10-11	34.67077830951122	37.0	37.0	37.0	27.0	37.0
12-13	34.685139784021956	37.0	37.0	37.0	27.0	37.0
14-15	34.64594774893627	37.0	37.0	37.0	27.0	37.0
16-17	34.646510211213	37.0	37.0	37.0	27.0	37.0
18-19	34.65240205027777	37.0	37.0	37.0	27.0	37.0
20-21	34.73028438914915	37.0	37.0	37.0	27.0	37.0
22-23	34.79578695199636	37.0	37.0	37.0	27.0	37.0
24-25	34.83718398869843	37.0	37.0	37.0	27.0	37.0
26-27	34.82352232190408	37.0	37.0	37.0	27.0	37.0
28-29	34.86677492352568	37.0	37.0	37.0	27.0	37.0
30-31	34.9242778152545	37.0	37.0	37.0	27.0	37.0
32-33	34.93799086979839	37.0	37.0	37.0	27.0	37.0
34-35	34.93861966898815	37.0	37.0	37.0	27.0	37.0
36-37	34.88651716257402	37.0	37.0	37.0	27.0	37.0
38-39	34.877930736788215	37.0	37.0	37.0	27.0	37.0
40-41	34.84721581173024	37.0	37.0	37.0	27.0	37.0
42-43	34.8395823951176	37.0	37.0	37.0	27.0	37.0
44-45	34.86151001500522	37.0	37.0	37.0	27.0	37.0
46-47	34.85773721986669	37.0	37.0	37.0	27.0	37.0
48-49	34.82638695535993	37.0	37.0	37.0	27.0	37.0
50-51	34.80240289116822	37.0	37.0	37.0	27.0	37.0
52-53	34.77426295952327	37.0	37.0	37.0	27.0	37.0
54-55	34.721138304054776	37.0	37.0	37.0	27.0	37.0
56-57	34.6544603632273	37.0	37.0	37.0	27.0	37.0
58-59	34.588210342205045	37.0	37.0	37.0	27.0	37.0
60-61	34.55030300085771	37.0	37.0	37.0	27.0	37.0
62-63	34.592545599620294	37.0	37.0	37.0	27.0	37.0
64-65	34.52925925026208	37.0	37.0	37.0	27.0	37.0
66-67	34.50381764262903	37.0	37.0	37.0	27.0	37.0
68-69	34.4716788097476	37.0	37.0	37.0	27.0	37.0
70-71	34.4422691335278	37.0	37.0	37.0	27.0	37.0
72-73	34.42061900748766	37.0	37.0	37.0	27.0	37.0
74-75	34.32963933274409	37.0	37.0	37.0	27.0	37.0
76-77	34.29237200245913	37.0	37.0	37.0	27.0	37.0
78-79	34.2277327538041	37.0	37.0	37.0	27.0	37.0
80-81	34.16449966084085	37.0	37.0	37.0	27.0	37.0
82-83	34.12213746877026	37.0	37.0	37.0	27.0	37.0
84-85	34.09051625067505	37.0	37.0	37.0	27.0	37.0
86-87	34.03152311420974	37.0	37.0	37.0	27.0	37.0
88-89	33.96861236258449	37.0	37.0	37.0	27.0	37.0
90-91	33.90511672493726	37.0	37.0	37.0	24.5	37.0
92-93	33.76051159774791	37.0	35.0	37.0	22.0	37.0
94-95	33.684155942199055	37.0	33.0	37.0	22.0	37.0
96-97	33.58855230432012	37.0	33.0	37.0	22.0	37.0
98-99	33.54326007620336	37.0	33.0	37.0	22.0	37.0
100-101	33.42722653775505	37.0	33.0	37.0	22.0	37.0
102-103	33.34811743315388	37.0	33.0	37.0	22.0	37.0
104-105	33.229734073067775	37.0	33.0	37.0	14.0	37.0
106-107	33.128978579717355	37.0	33.0	37.0	14.0	37.0
108-109	33.0044520477551	37.0	33.0	37.0	14.0	37.0
110-111	32.915088751315054	37.0	33.0	37.0	14.0	37.0
112-113	32.84313282144906	37.0	33.0	37.0	14.0	37.0
114-115	32.68160617549944	37.0	33.0	37.0	14.0	37.0
116-117	32.483386807736935	37.0	33.0	37.0	14.0	37.0
118-119	32.36240883346071	37.0	33.0	37.0	14.0	37.0
120-121	32.27498145369403	37.0	33.0	37.0	14.0	37.0
122-123	32.07844853843897	37.0	33.0	37.0	14.0	37.0
124-125	30.44277647076411	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	904.0
1	68.0
2	31.0
3	31.0
4	20.0
5	18.0
6	19.0
7	25.0
8	14.0
9	12.0
10	14.0
11	12.0
12	9.0
13	14.0
14	30.0
15	161.0
16	294.0
17	498.0
18	1201.0
19	2272.0
20	3267.0
21	3947.0
22	4458.0
23	4876.0
24	5454.0
25	6172.0
26	7220.0
27	8651.0
28	10575.0
29	12597.0
30	15302.0
31	18453.0
32	23183.0
33	30625.0
34	45575.0
35	85066.0
36	244079.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.88896806495771	20.238528325238622	12.868903182252891	24.003600427550772
2	17.34340443078307	23.10829553548	36.206282565505866	23.34201746823106
3	19.73374011926224	27.43706124651902	28.528112081017365	24.30108655320138
4	14.65776375166789	19.829537788039158	34.0086730333878	31.50402542690514
5	15.246208926127949	37.57858629027723	31.179403550207123	15.995801233387693
6	28.88820980838056	35.83045401114733	18.683317054148755	16.59801912632335
7	25.826040330866086	33.64417647191163	21.576600898407914	18.953182298814372
8	25.341202231687564	34.12509748635191	20.53681234627152	19.996887935689003
9	26.283233959575504	18.78782765215435	20.047436907038662	34.88150148123149
10-11	24.994515930051392	27.546717713827167	25.5188305150526	21.93993584106884
12-13	25.816391481686672	26.533156719978777	25.12671820701822	22.523733591316322
14-15	24.298934012245923	26.660192500215597	23.61736551419005	25.423507973348432
16-17	23.702926420229637	28.471581353732862	24.419377052405736	23.40611517363177
18-19	23.914381569196554	27.68976950253282	25.714291067813267	22.68155786045736
20-21	25.678018732454134	26.287727423027675	25.250595049079255	22.783658795438942
22-23	25.71275493669084	26.163852613949935	24.872379446581082	23.251013002778148
24-25	24.59428629227121	26.850155654705045	25.049730835365647	23.505827217658098
26-27	23.978140643511733	27.076474968039822	25.088715833048465	23.85666855539998
28-29	23.81190956016212	26.94990298244694	25.379173814999888	23.85901364239105
30-31	24.375402094120226	26.58667393362532	25.40963265336701	23.62829131888744
32-33	23.909820906004917	26.928432634905768	25.243310312536583	23.918436146552732
34-35	24.220267518810797	26.88243745188426	25.27989075971821	23.617404269586732
36-37	24.227522894540428	26.939562533771728	25.00161542416356	23.83129914752428
38-39	24.0882159068778	26.907649323605636	25.176120956989408	23.828013812527153
40-41	23.810241824231873	26.778514627364412	25.470635227612227	23.94060832079149
42-43	23.963670891765553	26.769822006472495	25.258169273642572	24.00833782811938
44-45	23.9810861423221	26.866573033707862	25.208801498127343	23.943539325842696
46-47	24.068197205176876	26.791571893701953	25.19816874753606	23.942062153585113
48-49	24.034194916642868	26.866511547338707	25.25217814690144	23.84711538911699
50-51	23.64121554876193	27.129153226372782	25.306485517254295	23.923145707610992
52-53	23.716246530870684	26.889346600089358	25.20449481140956	24.189912057630398
54-55	23.61022528787304	26.73700181223045	25.330954488197087	24.32181841169942
56-57	23.834627801103068	26.62133141777702	24.965979832338874	24.578060948781033
58-59	23.819927982431086	26.739214924212977	25.266085718700687	24.174771374655247
60-61	23.986681090801802	26.70628620668662	25.483793922193964	23.823238780317613
62-63	23.632490038254975	26.632859945590848	25.418814704518955	24.315835311635226
64-65	23.51739639428705	26.41414188714587	25.496511355654413	24.571950362912666
66-67	23.94213452682979	26.234726588729774	25.283506916641613	24.539631967798822
68-69	23.6460107710446	26.332965932895885	25.263165780629166	24.757857515430352
70-71	23.7110525093459	26.39907552385733	25.260149384556602	24.62972258224017
72-73	23.96337272133039	26.46281552070632	25.354969574036513	24.218842183926775
74-75	23.91824458267824	26.271049154569127	25.473421623590596	24.337284639162032
76-77	23.61381901406736	26.23662982670045	25.501235178328287	24.6483159809039
78-79	23.67270602206085	26.358480352583975	25.46690591829357	24.50190770706161
80-81	23.562038163356846	26.56526979079169	25.549709097344937	24.32298294850653
82-83	23.717771611014385	26.264480473077956	25.741408650680743	24.276339265226916
84-85	23.5813740086332	26.622470668651744	25.312602413925482	24.483552908789573
86-87	23.30093723959065	26.717882456485302	25.520444182279522	24.460736121644523
88-89	23.60975079917676	26.13565498785551	25.641354564811504	24.61323964815623
90-91	24.003509123440065	26.129861910187785	25.474756875232313	24.391872091139838
92-93	23.765930090675916	26.367747354778537	25.53562281515804	24.33069973938751
94-95	23.20238121989768	26.543598271199897	25.610005018613812	24.644015490288606
96-97	23.371168998472093	26.420709877467868	25.421297821983885	24.786823302076154
98-99	23.037618226907664	26.66931291478301	25.481028928672067	24.812039929637262
100-101	23.519946532173368	26.852793113583562	25.23995933749319	24.38730101674988
102-103	23.502367565199318	26.692261460082793	25.513908626400273	24.291462348317616
104-105	23.50686711470717	26.75127573588265	25.481016319870587	24.260840829539593
106-107	23.45265254882283	26.67517943753926	25.613673107726182	24.258494905911725
108-109	23.35995476447476	26.74728301318028	25.495161427727542	24.39760079461742
110-111	23.09507650398551	27.276139836835185	25.39979124639011	24.228992412789204
112-113	23.083137375310788	27.314426504508283	25.17027753647066	24.432158583710272
114-115	23.134030075680634	27.322066263847873	25.18316398406822	24.360739676403274
116-117	23.184752133231605	27.15726702957738	25.238271321784474	24.41970951540654
118-119	22.97433391924853	26.920463415866884	25.49489242933919	24.610310235545395
120-121	23.01240895931168	27.01731001618397	25.62458990153893	24.34569112296542
122-123	22.92843510467923	27.280811038237147	25.6147980220132	24.175955835070422
124-125	23.0941091241279	27.03046991851351	25.287954488870252	24.587466468488334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1962.0
1	1279.0
2	492.0
3	342.0
4	247.0
5	190.5
6	181.5
7	175.5
8	162.0
9	145.5
10	143.5
11	133.5
12	121.5
13	123.0
14	133.0
15	139.0
16	146.5
17	168.5
18	190.0
19	218.5
20	259.5
21	303.5
22	370.0
23	453.0
24	547.0
25	717.5
26	1012.5
27	1370.5
28	1792.5
29	2592.5
30	3795.0
31	4817.5
32	6079.0
33	7506.0
34	8829.0
35	9959.0
36	10872.5
37	12120.0
38	12619.0
39	12703.0
40	12955.0
41	12966.0
42	13376.0
43	14355.5
44	15596.0
45	16952.0
46	18459.5
47	19821.0
48	21068.0
49	22178.0
50	23132.5
51	23218.0
52	22180.5
53	21726.5
54	21364.0
55	20426.0
56	19543.0
57	17913.0
58	15861.0
59	13945.0
60	12086.5
61	10169.0
62	8291.5
63	6645.5
64	5161.0
65	4066.0
66	3190.5
67	2536.5
68	2104.0
69	1747.0
70	1442.5
71	1227.0
72	1058.0
73	917.5
74	785.5
75	671.5
76	516.5
77	370.5
78	256.5
79	157.0
80	101.0
81	64.5
82	47.0
83	31.0
84	14.0
85	5.0
86	4.0
87	2.5
88	1.5
89	1.5
90	1.5
91	1.5
92	1.0
93	1.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35074474863915894
2	0.3002913218237232
3	0.2864633455854186
4	0.2875845328479838
5	0.30963454901176685
6	0.3262654934064846
7	0.3286947324753759
8	0.32495744160015844
9	0.33766423057589784
10-11	0.33336634606939775
12-13	0.33233859107871294
14-15	0.3260786288627237
16-17	0.2755317697754075
18-19	0.27160761435642916
20-21	0.2555372635929941
22-23	0.25002475955204834
24-25	0.23787856420759154
26-27	0.23909318374203725
28-29	0.22853533701954787
30-31	0.22862876929142834
32-33	0.22601266567877612
34-35	0.2388128869263959
36-37	0.2301236856415153
38-39	0.21283871534363455
40-41	0.23685080921690677
42-43	0.22330312979424344
44-45	0.21433363169372152
46-47	0.2233965620661239
48-49	0.21480079305312372
50-51	0.21480079305312372
52-53	0.24881014001760263
54-55	0.23815886102323286
56-57	0.23778513193571113
58-59	0.23348724742921104
60-61	0.24656776549247217
62-63	0.22974995655399358
64-65	0.2376916996638307
66-67	0.23423470560425455
68-69	0.22713385294134136
70-71	0.22853533701954787
72-73	0.22946965973835226
74-75	0.22208851025979776
76-77	0.22722728521322177
78-79	0.2354493251387002
80-81	0.2252652075037326
82-83	0.23946691282955898
84-85	0.21713659985013464
86-87	0.21153066353730843
88-89	0.21676287076261286
90-91	0.20751307584644965
92-93	0.22657325931005873
94-95	0.21209125716859104
96-97	0.20237430089302563
98-99	0.1976092550271234
100-101	0.18574335649830792
102-103	0.19695522912396035
104-105	0.19499315141447116
106-107	0.19574060958951464
108-109	0.19686179685207988
110-111	0.19144272508301458
112-113	0.19041497009232977
114-115	0.1858367887701884
116-117	0.19424569323942767
118-119	0.1756526711352208
120-121	0.18247322698249266
122-123	0.1807914460886448
124-125	0.1771475874853078
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	535147.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.3237388792399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.74007654884699	67.84390768307566
2	8.550686840409293	13.22342152971922
3	1.8205462429461736	4.223143269214536
4	0.6605137089043772	2.0429355821392137
5	0.32955072633410415	1.2741047155261058
6	0.20137613451766623	0.9342693385172827
7	0.1337197557532707	0.7237798033803148
8	0.09521629753769585	0.5889984102282245
9	0.05849737491593922	0.4070912168808974
>10	0.37180932747627105	5.3290687927964475
>50	0.02561956263584798	1.3348894558716486
>100	0.011542878832150997	1.3732032110734407
>500	5.630672601049177E-4	0.303463106568858
>1k	2.8153363005245887E-4	0.39772388500810874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1827	0.3414015214511153	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	883	0.16500139214085102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	9.343227188043659E-5	0.0
12-13	0.0	0.0	0.0	1.8686454376087318E-4	0.0
14-15	0.0	0.0	0.0	1.8686454376087318E-4	0.0
16-17	0.0	0.0	0.0	1.8686454376087318E-4	1.8686454376087318E-4
18-19	0.0	0.0	0.0	2.802968156413098E-4	1.8686454376087318E-4
20-21	0.0	0.0	0.0	0.0010277549906848025	1.8686454376087318E-4
22-23	0.0	0.0	0.0	0.002335806797010915	1.8686454376087318E-4
24-25	0.0	0.0	0.0	0.0057928008565870685	1.8686454376087318E-4
26-27	0.0	0.0	0.0	0.009716956275565406	3.7372908752174636E-4
28-29	0.0	0.0	0.0	0.015509757132152472	3.7372908752174636E-4
30-31	0.0	0.0	0.0	0.029711462457978836	3.7372908752174636E-4
32-33	0.0	0.0	0.0	0.055031608137577154	3.7372908752174636E-4
34-35	0.0	0.0	0.0	0.08194010243914289	7.474581750434927E-4
36-37	0.0	0.0	0.0	0.12398462478533935	7.474581750434927E-4
38-39	0.0	0.0	0.0	0.1892003505578841	7.474581750434927E-4
40-41	0.0	0.0	0.0	0.252734295436581	9.343227188043659E-4
42-43	0.0	0.0	0.0	0.31496018850895174	9.343227188043659E-4
44-45	0.0	0.0	0.0	0.3778401074844856	9.343227188043659E-4
46-47	0.0	0.0	0.0	0.4554823254171284	0.0010277549906848025
48-49	0.0	0.0	0.0	0.5322836529028473	0.0011211872625652391
50-51	0.0	0.0	0.0	0.6049739604258269	0.0011211872625652391
52-53	0.0	0.0	0.0	0.671778034820339	0.0011211872625652391
54-55	0.0	0.0	0.0	0.7496071172967427	0.0011211872625652391
56-57	0.0	0.0	0.0	0.8224842893634834	0.0011211872625652391
58-59	0.0	0.0	0.0	0.9126464317281047	0.0012146195344456758
60-61	0.0	0.0	0.0	1.001967683645802	0.0014949163500869854
62-63	0.0	0.0	0.0	1.0894202901258905	0.0016817808938478587
64-65	0.0	0.0	0.0	1.1689311534961422	0.0016817808938478587
66-67	0.0	0.0	0.0	1.249376339585198	0.0016817808938478587
68-69	0.0	0.0	0.0	1.3553285358976133	0.0016817808938478587
70-71	0.0	0.0	0.0	1.4601595449474631	0.0016817808938478587
72-73	0.0	0.0	0.0	1.569568735319454	0.0018686454376087317
74-75	0.0	0.0	0.0	1.670101859862804	0.0018686454376087317
76-77	0.0	0.0	0.0	1.773437952562567	0.0018686454376087317
78-79	0.0	0.0	0.0	1.8916297764913192	0.0018686454376087317
80-81	0.0	0.0	0.0	2.02234152485205	0.0018686454376087317
82-83	0.0	0.0	0.0	2.1539875959315853	0.0018686454376087317
84-85	0.0	0.0	0.0	2.280401459785816	0.0018686454376087317
86-87	0.0	0.0	0.0	2.407375917271329	0.0019620777094891684
88-89	0.0	0.0	0.0	2.5521025064141254	0.002055509981369605
90-91	0.0	0.0	0.0	2.7100964781639436	0.002055509981369605
92-93	0.0	0.0	0.0	2.8561311191130665	0.002055509981369605
94-95	0.0	0.0	0.0	3.005622754121765	0.002055509981369605
96-97	0.0	0.0	0.0	3.151377098255246	0.002055509981369605
98-99	0.0	0.0	0.0	3.3212369685338796	0.002055509981369605
100-101	0.0	0.0	0.0	3.4969830719409805	0.002055509981369605
102-103	0.0	0.0	0.0	3.684968802964419	0.002055509981369605
104-105	0.0	0.0	0.0	3.8829517870790644	0.002055509981369605
106-107	0.0	0.0	0.0	4.0883159206722635	0.002055509981369605
108-109	0.0	0.0	0.0	4.293773486537344	0.0022423745251304783
110-111	0.0	0.0	0.0	4.50493042098713	0.002522671340771788
112-113	0.0	0.0	0.0	4.724029098546755	0.0026161036126522244
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2340	0.0	33.60882	1
GTATCAA	3010	0.0	26.120443	2
CATGGGG	2555	0.0	23.778385	4
GTACATA	215	3.3773995E-6	22.169033	1
CAACGCA	3615	0.0	21.574095	6
ATCAACG	3560	0.0	21.41574	4
ATGGGGA	840	0.0	21.26635	5
TATCAAC	3650	0.0	21.21405	3
AACGCAG	3705	0.0	21.212702	7
TCAACGC	3655	0.0	21.016157	5
ATGGGGG	1450	0.0	20.943687	5
GTACATG	5440	0.0	20.589897	1
GTCTAGA	145	0.0038350068	20.544579	1
TACATGG	5480	0.0	20.107788	2
ACATGGG	5480	0.0	19.346952	3
ACGCAGA	4070	0.0	19.310335	8
TCGCATG	155	0.0056498665	19.213717	2
CATGGGT	840	0.0	19.145098	4
CGCAGAG	4170	0.0	18.704475	9
TCTACAC	255	1.4805613E-5	18.686283	3
>>END_MODULE