FastQCFastQC Report
Sun 1 Jan 2017
ERR1142603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535147
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31300.584886021971533No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28210.5271448779494233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.2890794491980708No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8720.16294588215948141No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6370.11903271437567621No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.11697720439430662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG1550.061.410341
GGTATCA26950.047.9020541
GTATCAA38550.037.6546481
TATCAAC47500.030.428792
ATCAACG48450.029.9549123
GTTCGCG400.005373251629.73729110-111
CAACGCA49550.029.2899175
TCAACGC50100.028.849654
AACGCAG52050.027.994766
ACGCAGA55400.026.0872277
GAGTACT24400.025.5935712-13
CGCAGAG56800.025.3395238
GCGCAGA2351.0397343E-825.315431
GTAAGTA3002.237357E-1023.7920552
GTACACT3501.8189894E-1223.7898336
GCAGAGT61500.023.4030089
GTACTTT27700.022.54451814-15
CAGAGTA61300.022.21807510-11
AGTACTT25700.020.24912812-13
ACTTTTT31900.019.76271216-17
Produced by FastQC (version 0.11.5)