##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142603_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	535147
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.237078783960296	27.0	14.0	33.0	14.0	33.0
2	28.963469850340186	33.0	27.0	33.0	27.0	33.0
3	31.27221679276909	33.0	33.0	33.0	27.0	33.0
4	30.995618026448806	33.0	33.0	33.0	27.0	33.0
5	31.8659658000512	33.0	33.0	33.0	27.0	33.0
6	34.838700394471054	37.0	37.0	37.0	27.0	37.0
7	35.59377703696367	37.0	37.0	37.0	33.0	37.0
8	35.39005731135557	37.0	37.0	37.0	33.0	37.0
9	35.85737750561995	37.0	37.0	37.0	33.0	37.0
10-11	35.94837306384974	37.0	37.0	37.0	33.0	37.0
12-13	36.033997200769136	37.0	37.0	37.0	33.0	37.0
14-15	35.97382868632357	37.0	37.0	37.0	33.0	37.0
16-17	35.99699708678176	37.0	37.0	37.0	33.0	37.0
18-19	36.00041857657803	37.0	37.0	37.0	33.0	37.0
20-21	36.028668758303795	37.0	37.0	37.0	37.0	37.0
22-23	36.038324983602635	37.0	37.0	37.0	37.0	37.0
24-25	36.02644133294216	37.0	37.0	37.0	37.0	37.0
26-27	35.976147675311644	37.0	37.0	37.0	35.0	37.0
28-29	35.96286721218656	37.0	37.0	37.0	33.0	37.0
30-31	35.94207479440229	37.0	37.0	37.0	33.0	37.0
32-33	35.88565104541369	37.0	37.0	37.0	33.0	37.0
34-35	35.77227658942309	37.0	37.0	37.0	33.0	37.0
36-37	35.69054857824112	37.0	37.0	37.0	33.0	37.0
38-39	35.68015143502626	37.0	37.0	37.0	33.0	37.0
40-41	35.5757221847455	37.0	37.0	37.0	33.0	37.0
42-43	35.53902665996446	37.0	37.0	37.0	33.0	37.0
44-45	35.436138107847	37.0	37.0	37.0	33.0	37.0
46-47	35.29806483078481	37.0	37.0	37.0	33.0	37.0
48-49	35.13798077911303	37.0	37.0	37.0	33.0	37.0
50-51	35.04059538780933	37.0	37.0	37.0	33.0	37.0
52-53	34.93997910854401	37.0	37.0	37.0	27.0	37.0
54-55	34.89129248598983	37.0	37.0	37.0	27.0	37.0
56-57	34.87768968152676	37.0	37.0	37.0	27.0	37.0
58-59	34.831286543697345	37.0	37.0	37.0	27.0	37.0
60-61	34.78875056760105	37.0	37.0	37.0	27.0	37.0
62-63	34.77763866750631	37.0	37.0	37.0	27.0	37.0
64-65	34.74202882572452	37.0	37.0	37.0	27.0	37.0
66-67	34.71557721523245	37.0	37.0	37.0	27.0	37.0
68-69	34.68510895137224	37.0	37.0	37.0	27.0	37.0
70-71	34.56091503829789	37.0	37.0	37.0	27.0	37.0
72-73	34.57802061863376	37.0	37.0	37.0	27.0	37.0
74-75	34.50582456782902	37.0	37.0	37.0	27.0	37.0
76-77	34.390085341037135	37.0	37.0	37.0	27.0	37.0
78-79	34.39366846866375	37.0	37.0	37.0	27.0	37.0
80-81	34.38448781362878	37.0	37.0	37.0	27.0	37.0
82-83	34.308548866012515	37.0	37.0	37.0	27.0	37.0
84-85	34.23525872330407	37.0	37.0	37.0	27.0	37.0
86-87	34.09865420155583	37.0	37.0	37.0	27.0	37.0
88-89	34.0179567483327	37.0	37.0	37.0	27.0	37.0
90-91	33.951240500273755	37.0	37.0	37.0	24.5	37.0
92-93	33.77799744743034	37.0	35.0	37.0	22.0	37.0
94-95	33.81747818823613	37.0	35.0	37.0	22.0	37.0
96-97	33.812267470433355	37.0	35.0	37.0	22.0	37.0
98-99	33.77655578747522	37.0	33.0	37.0	22.0	37.0
100-101	33.6456609118616	37.0	33.0	37.0	22.0	37.0
102-103	33.57185689165781	37.0	33.0	37.0	22.0	37.0
104-105	33.41824769642734	37.0	33.0	37.0	18.0	37.0
106-107	33.37931914034836	37.0	33.0	37.0	18.0	37.0
108-109	33.25176727142262	37.0	33.0	37.0	14.0	37.0
110-111	33.17239095052388	37.0	33.0	37.0	14.0	37.0
112-113	33.029270462134704	37.0	33.0	37.0	14.0	37.0
114-115	32.996931684191445	37.0	33.0	37.0	14.0	37.0
116-117	32.853462693428156	37.0	33.0	37.0	14.0	37.0
118-119	32.73875776188599	37.0	33.0	37.0	14.0	37.0
120-121	32.63047256174472	37.0	33.0	37.0	14.0	37.0
122-123	32.55796351282918	37.0	33.0	37.0	14.0	37.0
124-125	31.14764728196178	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	8.0
15	246.0
16	80.0
17	45.0
18	144.0
19	474.0
20	1341.0
21	2955.0
22	4613.0
23	6395.0
24	5845.0
25	4894.0
26	4847.0
27	5836.0
28	7103.0
29	9276.0
30	12073.0
31	16318.0
32	22051.0
33	32638.0
34	52727.0
35	105974.0
36	239264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.26681395170621	15.19839556500074	12.779220099548239	21.755570383744814
2	18.67823233616184	21.055149332800145	37.55043006874747	22.716188262290547
3	20.460771453024034	26.65347400522474	29.09617188580313	23.789582655948095
4	14.998420982802726	19.202717778238714	34.17348260184661	31.62537863711195
5	16.087709692429303	35.18646905699374	32.393310771795214	16.33251047878175
6	29.049064163128623	34.54916133468875	19.032544997907074	17.36922950427555
7	25.811973158776937	32.38362543375932	22.88941169435688	18.914989713106866
8	25.91586984510798	31.937953496889637	21.223514286728694	20.922662371273688
9	26.835617129498996	16.337567061013143	21.107845134140714	35.71897067534715
10-11	25.562415560584288	25.328367719523794	26.48608700039428	22.623129719497634
12-13	26.839965168383955	24.546572337268707	26.457639597418275	22.155822896929063
14-15	24.832802949469958	24.709939511947184	25.554006656115046	24.90325088246781
16-17	23.29163762480216	26.728170016836494	26.648378856650602	23.331813501710744
18-19	23.184564241227175	25.203075042932127	29.161333241146824	22.45102747469387
20-21	24.728951459794	23.953085524643832	29.211670129908956	22.106292885653207
22-23	24.863915896006144	23.884465389883527	28.844037245840866	22.407581468269466
24-25	23.755155125601	24.607350877422466	28.97643077509544	22.66106322188109
26-27	23.184751105770935	24.72582299816686	29.152457175318187	22.93696872074402
28-29	22.983296053578204	24.667145043474363	29.370934497881873	22.978624405065563
30-31	23.50132160072055	24.292319953508056	29.43539759673286	22.770960849038534
32-33	23.25535765506764	24.6236771121125	29.02677869850349	23.094186534316368
34-35	23.363393609606334	24.629027164498726	29.18450444457317	22.82307478132177
36-37	23.480205105972665	24.670739136447047	28.747164282857877	23.101891474722407
38-39	23.387031974392084	24.66826871868851	28.91635382427632	23.028345482643086
40-41	23.124201131683396	24.669240486459415	29.090018911221904	23.116539470635285
42-43	23.490543031705112	24.51224878979023	28.770337176915334	23.22687100158932
44-45	23.39247290228003	24.843477440289714	28.50406384046238	23.259985816967877
46-47	23.438980317557608	24.814863953268915	28.258684062509925	23.487471666663552
48-49	23.567636555936964	25.043212425744706	28.146845633069045	23.24230538524929
50-51	23.296963264299343	25.32313551229849	28.069390279680164	23.310510943722004
52-53	23.37114848817241	25.133281135837443	27.75181398755856	23.74375638843159
54-55	23.261365568712897	25.143091524384886	27.740134953573502	23.85540795332871
56-57	23.408241100108942	25.144679873006858	27.39051139219691	24.056567634687294
58-59	23.34564147794905	25.150566106135326	27.68743915223294	23.81635326368269
60-61	23.58267915170972	24.99378675391995	28.043883269456803	23.37965082491353
62-63	23.083844252140064	25.059282776508134	28.082751094559065	23.77412187679273
64-65	23.049367743816184	24.802250596565056	28.224020689642288	23.924360969976473
66-67	23.488219124838597	24.74095902621149	27.975117117352806	23.795704731597112
68-69	23.21558375549148	24.76740035915365	28.166372977892056	23.85064290746281
70-71	23.246136108396385	24.8674663223376	28.27438068418584	23.612016885080177
72-73	23.42683412221315	24.847284951611424	28.33940954541462	23.386471380760803
74-75	23.24662526669696	24.914217542442447	28.399556455609098	23.439600735251496
76-77	23.27115012900155	24.819168723179228	28.28290007078954	23.626781077029683
78-79	23.190026646933735	24.990002728227438	28.296296711551616	23.523673913287215
80-81	22.970417474077216	25.09674911753219	28.521602475581474	23.411230932809115
82-83	23.14055764117149	24.943239894832637	28.530198244594473	23.3860042194014
84-85	23.178403000871388	25.02374817213872	28.137283134309936	23.660565692679953
86-87	22.90608287988172	25.33698901890694	28.115715548984472	23.641212552226865
88-89	23.183985996639567	24.879366104566536	28.0937738401127	23.842874058681193
90-91	23.504808568930613	24.854574101519948	27.997512728564818	23.643104600984625
92-93	23.247629124649528	25.13319655835997	27.91098213224886	23.708192184741637
94-95	22.7673428429677	25.094951522167502	28.206745318761488	23.93096031610331
96-97	22.96303632459866	25.067317951889855	27.879162174131594	24.09048354937989
98-99	22.80037073925482	25.303421302931717	27.650346540296404	24.245861417517055
100-101	23.077303993108437	25.401058027046773	27.432742779086865	24.088895200757925
102-103	23.307097502363842	25.28506239418775	27.50548448460794	23.902355618840467
104-105	23.29858512470206	25.291912048443805	27.52648628244622	23.883016544407912
106-107	23.240386735582437	25.25273476770825	27.50436329525027	24.00251520145904
108-109	23.223166826903764	25.217955150431333	27.261421915015475	24.297456107649428
110-111	22.925239093630523	25.778759441348715	27.19033684265602	24.10566462236474
112-113	22.95635209830588	25.7679212775579	27.046824604874182	24.228902019262033
114-115	22.994939698699046	25.8346320443393	27.00636835555157	24.16405990141008
116-117	23.05728068347767	25.72085535616015	26.928564287656346	24.293299672705835
118-119	22.90984414519042	25.518667353084346	27.228949883723214	24.342538618002017
120-121	23.008185608987443	25.635129819591455	27.309437696032766	24.04724687538833
122-123	22.903706831954583	25.75114874978277	27.310253070651612	24.03489134761103
124-125	22.87677453231952	25.567093532358758	27.283370177205633	24.272761758116086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	322.0
1	273.5
2	422.5
3	593.5
4	423.0
5	356.5
6	516.5
7	988.5
8	1630.0
9	1970.0
10	1746.5
11	1300.5
12	1124.0
13	991.0
14	918.5
15	967.0
16	937.0
17	893.5
18	884.5
19	856.5
20	822.5
21	781.5
22	829.0
23	867.5
24	915.5
25	1037.0
26	1191.0
27	1455.0
28	1803.0
29	2468.0
30	3466.0
31	4414.5
32	5640.5
33	6883.0
34	8183.0
35	9344.0
36	10252.5
37	11377.0
38	12110.5
39	12433.5
40	12330.5
41	12227.5
42	12727.5
43	13851.5
44	15071.0
45	16204.5
46	17708.0
47	19297.5
48	20480.0
49	21634.5
50	22883.5
51	22795.0
52	21621.5
53	21153.5
54	21006.0
55	20176.0
56	19187.5
57	17605.0
58	15753.0
59	14076.5
60	12230.0
61	10237.5
62	8289.0
63	6632.0
64	5058.5
65	3960.5
66	3125.0
67	2398.0
68	2001.5
69	1672.5
70	1356.5
71	1145.0
72	1002.0
73	947.0
74	800.0
75	650.5
76	493.5
77	361.0
78	274.0
79	158.0
80	106.0
81	86.0
82	56.0
83	27.0
84	15.0
85	9.0
86	9.0
87	8.5
88	6.5
89	4.0
90	3.0
91	3.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024292390688913514
2	0.0
3	1.8686454376087318E-4
4	7.474581750434927E-4
5	0.003363561787695717
6	0.002055509981369605
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.8686454376087318E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.605936312826196E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	7.474581750434927E-4
30-31	9.343227188043659E-5
32-33	2.802968156413098E-4
34-35	0.0
36-37	0.0016817808938478585
38-39	0.0
40-41	0.002802968156413098
42-43	0.002335806797010915
44-45	9.343227188043659E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.06801869392895783
76-77	0.08661171603316471
78-79	1.8686454376087318E-4
80-81	0.0
82-83	0.0
84-85	0.0689530166477622
86-87	0.15313549361203557
88-89	0.18490246605138402
90-91	0.07932399882649067
92-93	0.12940369655440467
94-95	0.0010277549906848025
96-97	0.0
98-99	0.0
100-101	0.0
102-103	1.8686454376087318E-4
104-105	0.0021489422532500416
106-107	1.8686454376087318E-4
108-109	4.6716135940218294E-4
110-111	1.8686454376087318E-4
112-113	1.8686454376087318E-4
114-115	1.8686454376087318E-4
116-117	2.802968156413098E-4
118-119	2.802968156413098E-4
120-121	9.343227188043659E-5
122-123	0.0
124-125	0.0011211872625652391
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	535147.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.98154656541436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.00008414166133	64.36400776121285
2	8.901707739276068	13.17124211249924
3	1.9697283440670872	4.371706476234473
4	0.710507606272537	2.1025780623413115
5	0.37327011452037234	1.3807550179433241
6	0.21849225092697988	0.9698636781681936
7	0.145090540252765	0.7513815791937744
8	0.11104457401287092	0.6572199458535851
9	0.08164156023137431	0.543597200093745
>10	0.4366511972693036	6.014267103369218
>50	0.034424860199626886	1.6879534495502644
>100	0.015621364178737883	1.923800280212195
>500	8.678535654854315E-4	0.45692664348125683
>1k	8.678535654854315E-4	1.6047006898465874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3130	0.584886021971533	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2821	0.5271448779494233	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1547	0.2890794491980708	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	872	0.16294588215948141	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	637	0.11903271437567621	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	626	0.11697720439430662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	9.343227188043659E-5	0.0
12-13	0.0	0.0	0.0	1.8686454376087318E-4	0.0
14-15	0.0	0.0	0.0	1.8686454376087318E-4	0.0
16-17	0.0	0.0	0.0	1.8686454376087318E-4	0.0
18-19	0.0	0.0	0.0	2.802968156413098E-4	9.343227188043659E-5
20-21	0.0	0.0	0.0	0.0010277549906848025	1.8686454376087318E-4
22-23	0.0	0.0	0.0	0.002335806797010915	1.8686454376087318E-4
24-25	0.0	0.0	0.0	0.005699368584706632	1.8686454376087318E-4
26-27	0.0	0.0	0.0	0.009716956275565406	1.8686454376087318E-4
28-29	0.0	0.0	0.0	0.015509757132152472	1.8686454376087318E-4
30-31	0.0	0.0	0.0	0.029711462457978836	1.8686454376087318E-4
32-33	0.0	0.0	0.0	0.055498769496979336	1.8686454376087318E-4
34-35	0.0	0.0	0.0	0.08240726379854507	1.8686454376087318E-4
36-37	0.0	0.0	0.0	0.12454521841662197	1.8686454376087318E-4
38-39	0.0	0.0	0.0	0.1889200537422428	1.8686454376087318E-4
40-41	0.0	0.0	0.0	0.25170654044589613	1.8686454376087318E-4
42-43	0.0	0.0	0.0	0.3142127303339083	1.8686454376087318E-4
44-45	0.0	0.0	0.0	0.37774667521260513	1.8686454376087318E-4
46-47	0.0	0.0	0.0	0.4561363513202914	1.8686454376087318E-4
48-49	0.0	0.0	0.0	0.5331245433497712	1.8686454376087318E-4
50-51	0.0	0.0	0.0	0.6060017154165118	1.8686454376087318E-4
52-53	0.0	0.0	0.0	0.6736466802579477	2.802968156413098E-4
54-55	0.0	0.0	0.0	0.7524100854531559	3.7372908752174636E-4
56-57	0.0	0.0	0.0	0.8257544188792986	3.7372908752174636E-4
58-59	0.0	0.0	0.0	0.9164771548752025	3.7372908752174636E-4
60-61	0.0	0.0	0.0	1.0066392972398237	3.7372908752174636E-4
62-63	0.0	0.0	0.0	1.0944656328074343	3.7372908752174636E-4
64-65	0.0	0.0	0.0	1.1746305220808488	3.7372908752174636E-4
66-67	0.0	0.0	0.0	1.256010030888709	3.7372908752174636E-4
68-69	0.0	0.0	0.0	1.3618687949292438	3.7372908752174636E-4
70-71	0.0	0.0	0.0	1.466793236250974	3.7372908752174636E-4
72-73	0.0	0.0	0.0	1.5760155620792045	3.7372908752174636E-4
74-75	0.0	0.0	0.0	1.6771092802538368	3.7372908752174636E-4
76-77	0.0	0.0	0.0	1.7797913470504365	3.7372908752174636E-4
78-79	0.0	0.0	0.0	1.8981700355229498	3.7372908752174636E-4
80-81	0.0	0.0	0.0	2.028321190252398	3.7372908752174636E-4
82-83	0.0	0.0	0.0	2.1602475581475744	3.7372908752174636E-4
84-85	0.0	0.0	0.0	2.2846993442923162	3.7372908752174636E-4
86-87	0.0	0.0	0.0	2.4118606663215902	3.7372908752174636E-4
88-89	0.0	0.0	0.0	2.5570544168237888	3.7372908752174636E-4
90-91	0.0	0.0	0.0	2.7169170340112156	3.7372908752174636E-4
92-93	0.0	0.0	0.0	2.86341883631974	3.7372908752174636E-4
94-95	0.0	0.0	0.0	3.0124433099690364	3.7372908752174636E-4
96-97	0.0	0.0	0.0	3.159972867268246	3.7372908752174636E-4
98-99	0.0	0.0	0.0	3.3308604925375644	3.7372908752174636E-4
100-101	0.0	0.0	0.0	3.506513163672785	3.7372908752174636E-4
102-103	0.0	0.0	0.0	3.6945923269681042	5.605936312826196E-4
104-105	0.0	0.0	0.0	3.8948176856078796	7.474581750434927E-4
106-107	0.0	0.0	0.0	4.101583303279286	7.474581750434927E-4
108-109	0.0	0.0	0.0	4.3078817595912895	7.474581750434927E-4
110-111	0.0	0.0	0.0	4.519879584488001	7.474581750434927E-4
112-113	0.0	0.0	0.0	4.738884829775744	7.474581750434927E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	155	0.0	61.41034	1
GGTATCA	2695	0.0	47.902054	1
GTATCAA	3855	0.0	37.654648	1
TATCAAC	4750	0.0	30.42879	2
ATCAACG	4845	0.0	29.954912	3
GTTCGCG	40	0.0053732516	29.73729	110-111
CAACGCA	4955	0.0	29.289917	5
TCAACGC	5010	0.0	28.84965	4
AACGCAG	5205	0.0	27.99476	6
ACGCAGA	5540	0.0	26.087227	7
GAGTACT	2440	0.0	25.59357	12-13
CGCAGAG	5680	0.0	25.339523	8
GCGCAGA	235	1.0397343E-8	25.31543	1
GTAAGTA	300	2.237357E-10	23.792055	2
GTACACT	350	1.8189894E-12	23.789833	6
GCAGAGT	6150	0.0	23.403008	9
GTACTTT	2770	0.0	22.544518	14-15
CAGAGTA	6130	0.0	22.218075	10-11
AGTACTT	2570	0.0	20.249128	12-13
ACTTTTT	3190	0.0	19.762712	16-17
>>END_MODULE