FastQCFastQC Report
Sun 1 Jan 2017
ERR1142593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297379
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4870.16376408556084995No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4120.13854374384203325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3970.13349967549826988No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA3520.11836747046697985No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3250.10928814744820582No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC3170.10659797766486537No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT600.001296640539.7305644
AAAACGC650.00192293136.6681825
ATGGGTG1254.8229456E-528.6011815
TTGTACG850.007151395528.0403737
ACGGTTA557.7464443E-427.04566446-47
GGTATCA18850.026.8870721
GTACAAA1608.570816E-626.086391
GGTAACA1159.907389E-425.9243621
GTAGGAC1651.0924898E-525.2830893
TCTAACG1200.001274858324.8274142
TAGGACT1951.4383859E-624.449584
TAGTACT2451.5832484E-824.3248354
ATTTAGG3002.1645974E-1023.8504141
CTAACGC1250.00161546923.8383393
CGCCTAC1501.666125E-423.8343167
GTATCAA21750.023.2864022
ATATTGG2052.2289223E-623.249076
TTTAGGG3103.274181E-1023.0654682
CTGTGCG2403.495661E-722.3446719
CTCGTCG2153.3658162E-622.171469
Produced by FastQC (version 0.11.5)