##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142593_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297379
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.093348891481913	33.0	33.0	33.0	27.0	33.0
2	31.167012465574235	33.0	33.0	33.0	27.0	33.0
3	31.27561125701546	33.0	33.0	33.0	27.0	33.0
4	31.173848859536147	33.0	33.0	33.0	27.0	33.0
5	31.25928192643058	33.0	33.0	33.0	27.0	33.0
6	34.429936209349016	37.0	37.0	37.0	27.0	37.0
7	34.560604481150314	37.0	37.0	37.0	27.0	37.0
8	34.530558647382634	37.0	37.0	37.0	27.0	37.0
9	34.363482290276046	37.0	37.0	37.0	27.0	37.0
10-11	34.483225110044756	37.0	37.0	37.0	27.0	37.0
12-13	34.497686453986326	37.0	37.0	37.0	27.0	37.0
14-15	34.43333086734437	37.0	37.0	37.0	27.0	37.0
16-17	34.442105864906395	37.0	37.0	37.0	27.0	37.0
18-19	34.45217550667667	37.0	37.0	37.0	27.0	37.0
20-21	34.540292017929985	37.0	37.0	37.0	27.0	37.0
22-23	34.63458751290441	37.0	37.0	37.0	27.0	37.0
24-25	34.7213757528272	37.0	37.0	37.0	27.0	37.0
26-27	34.70805773104355	37.0	37.0	37.0	27.0	37.0
28-29	34.761751838562915	37.0	37.0	37.0	27.0	37.0
30-31	34.83318089037894	37.0	37.0	37.0	27.0	37.0
32-33	34.85589433013091	37.0	37.0	37.0	27.0	37.0
34-35	34.84855689204685	37.0	37.0	37.0	27.0	37.0
36-37	34.799649605385724	37.0	37.0	37.0	27.0	37.0
38-39	34.80082655466593	37.0	37.0	37.0	27.0	37.0
40-41	34.76876309356074	37.0	37.0	37.0	27.0	37.0
42-43	34.75140813574596	37.0	37.0	37.0	27.0	37.0
44-45	34.768083825690454	37.0	37.0	37.0	27.0	37.0
46-47	34.74332417554703	37.0	37.0	37.0	27.0	37.0
48-49	34.72174060710407	37.0	37.0	37.0	27.0	37.0
50-51	34.68408495556176	37.0	37.0	37.0	27.0	37.0
52-53	34.64965414504723	37.0	37.0	37.0	27.0	37.0
54-55	34.58992565043261	37.0	37.0	37.0	27.0	37.0
56-57	34.518296517238944	37.0	37.0	37.0	27.0	37.0
58-59	34.45764832082965	37.0	37.0	37.0	27.0	37.0
60-61	34.41515540774567	37.0	37.0	37.0	27.0	37.0
62-63	34.44939958773148	37.0	37.0	37.0	27.0	37.0
64-65	34.37301389808964	37.0	37.0	37.0	27.0	37.0
66-67	34.34679483083876	37.0	37.0	37.0	27.0	37.0
68-69	34.29907962566287	37.0	37.0	37.0	27.0	37.0
70-71	34.27242676853443	37.0	37.0	37.0	27.0	37.0
72-73	34.213976104566896	37.0	37.0	37.0	27.0	37.0
74-75	34.14960370436379	37.0	37.0	37.0	27.0	37.0
76-77	34.09027705386056	37.0	37.0	37.0	27.0	37.0
78-79	34.02171975828824	37.0	37.0	37.0	27.0	37.0
80-81	33.95250337111901	37.0	37.0	37.0	27.0	37.0
82-83	33.906698186489294	37.0	37.0	37.0	27.0	37.0
84-85	33.88187968888187	37.0	37.0	37.0	24.5	37.0
86-87	33.805569660265185	37.0	37.0	37.0	22.0	37.0
88-89	33.74796808113552	37.0	37.0	37.0	22.0	37.0
90-91	33.6699363438575	37.0	33.0	37.0	22.0	37.0
92-93	33.53422568506855	37.0	33.0	37.0	22.0	37.0
94-95	33.45717585976145	37.0	33.0	37.0	22.0	37.0
96-97	33.37026992491064	37.0	33.0	37.0	14.0	37.0
98-99	33.31934837362423	37.0	33.0	37.0	14.0	37.0
100-101	33.19262624462387	37.0	33.0	37.0	14.0	37.0
102-103	33.123045003177765	37.0	33.0	37.0	14.0	37.0
104-105	33.00855305855491	37.0	33.0	37.0	14.0	37.0
106-107	32.91137740055619	37.0	33.0	37.0	14.0	37.0
108-109	32.76732385272666	37.0	33.0	37.0	14.0	37.0
110-111	32.68807145090945	37.0	33.0	37.0	14.0	37.0
112-113	32.60848277786932	37.0	33.0	37.0	14.0	37.0
114-115	32.460937053389785	37.0	33.0	37.0	14.0	37.0
116-117	32.25737190588441	37.0	33.0	37.0	14.0	37.0
118-119	32.133314726325665	37.0	33.0	37.0	14.0	37.0
120-121	32.0220022261155	37.0	33.0	37.0	14.0	37.0
122-123	31.819188644793343	37.0	27.0	37.0	14.0	37.0
124-125	30.168307109782468	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	506.0
1	49.0
2	15.0
3	24.0
4	10.0
5	9.0
6	9.0
7	6.0
8	8.0
9	13.0
10	7.0
11	11.0
12	13.0
13	9.0
14	32.0
15	157.0
16	258.0
17	380.0
18	890.0
19	1682.0
20	2595.0
21	3088.0
22	3175.0
23	2863.0
24	3116.0
25	3486.0
26	3958.0
27	4853.0
28	5859.0
29	7102.0
30	8246.0
31	9984.0
32	12574.0
33	16691.0
34	25009.0
35	47094.0
36	133598.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.045702753234835	20.78661923821995	12.277847900481259	23.88983010806396
2	16.987617341793072	23.33512107750377	36.40825330648344	23.26900827421972
3	19.531410725547797	28.542327837116833	28.118729068790955	23.80753236854442
4	14.166127030381523	19.88829528897523	34.614969510549884	31.330608170093356
5	14.639848591679963	38.279181018632535	31.64460877749927	15.436361612188234
6	28.371634775977896	37.18777098087908	18.386379509880996	16.05421473326203
7	25.1446754377381	34.285126183976544	22.096283823683244	18.473914554602114
8	24.781352912595153	34.935620579819684	20.373657074987854	19.909369432597313
9	25.8652690973394	18.848287685101443	20.153953105384648	35.132490112174516
10-11	24.574448161889386	28.118393591372374	25.411463790342854	21.895694456395383
12-13	25.511273235282705	27.169484061570444	24.87113771687248	22.448104986274377
14-15	23.926463170006258	27.072377799934877	23.607772081041002	25.39338694901786
16-17	23.356383005630054	28.663371929309843	24.475986692903163	23.50425837215694
18-19	23.507105968203977	28.039784654345944	25.936910400223912	22.516198977226168
20-21	25.195800447062233	26.710114193045925	25.328975094655142	22.765110265236697
22-23	25.295966960259598	26.66829617767289	24.933878376669895	23.101858485397614
24-25	24.44147048958451	27.31792907117659	25.062742394644182	23.177858044594714
26-27	23.824260137606483	27.488260299417146	25.203864577960715	23.48361498501566
28-29	23.50914802680365	27.143415712764092	25.773571168547505	23.57386509188475
30-31	24.079513950333276	26.901096308280874	25.715633979658048	23.3037557617278
32-33	23.662917977696623	27.38104667800318	25.222671450697128	23.733363893603073
34-35	23.686951597299238	27.245269789689775	25.553922543543557	23.51385606946743
36-37	23.820607245485263	27.341889489074468	25.29751320923256	23.539990056207706
38-39	23.761972486030693	27.351022168898837	25.520689399505258	23.36631594556521
40-41	23.3380919554566	27.040715714745605	25.852464735913326	23.768727593884467
42-43	23.68094210115656	27.279039159281222	25.512768011971747	23.527250727590467
44-45	23.702878672481077	27.458767785289183	25.358233299857787	23.480120242371957
46-47	23.704778467573963	27.308851186022526	25.27333454665561	23.713035799747896
48-49	23.83820273189605	27.156684652379205	25.537296481158815	23.467816134565933
50-51	23.432065730231052	27.41264727292335	25.332459356717262	23.822827640128335
52-53	23.088839666501467	27.598588059188234	25.05368948764889	24.258882786661406
54-55	23.226361031518625	27.40114613180516	25.010955671666945	24.36153716500927
56-57	23.658839155087207	27.040142122260203	24.790828125105342	24.510190597547247
58-59	23.436188537161694	26.932411524187376	25.614417706286257	24.01698223236467
60-61	23.56159857592696	27.144369449857393	25.371695199821993	23.922336774393656
62-63	23.221141691807258	26.94975814470868	25.387558357069423	24.441541806414644
64-65	23.016491036454227	26.853556090424274	25.389015864026483	24.740937009095017
66-67	23.694243682748578	26.42441546883849	25.353112105930258	24.52822874248267
68-69	23.294784244197412	26.28101847794579	25.2768995341751	25.1472977436817
70-71	23.32576043576745	26.325032358968826	25.441733631754936	24.90747357350879
72-73	23.769444678520266	26.304373472655264	25.584225162214548	24.34195668660993
74-75	23.659358622200504	26.413609477427073	25.52703864107447	24.399993259297954
76-77	23.421147539325958	26.33381307573301	25.693913113820287	24.551126271120744
78-79	23.32677132176217	26.362769123937746	25.726175347286595	24.58428420701349
80-81	23.00642756268306	26.63377048848802	25.728957063072354	24.630844885756563
82-83	23.574098982932	26.166998722344143	26.083226300158106	24.175675994565747
84-85	23.765534557280915	26.47012730964638	25.381212769722044	24.38312536335066
86-87	23.02626923012115	26.804724754410504	25.667683286434023	24.50132272903432
88-89	23.662902679502757	25.939662740908382	25.7334045575644	24.664030022024463
90-91	23.631431291439547	26.01447382831917	25.872262988955068	24.481831891286216
92-93	23.446318638446463	26.45888560638106	25.870393558270322	24.224402196902158
94-95	22.517449051465853	26.366013864876432	26.392301185288535	24.724235898369177
96-97	22.74140992765368	26.70141895830736	25.791782779082578	24.765388334956377
98-99	22.69981854971182	27.130945782249544	25.51036052504334	24.658875142995296
100-101	23.128315445998552	27.143321603105004	25.387156494947128	24.341206455949315
102-103	23.079152107768977	27.449853933682185	25.524541500018533	23.946452458530306
104-105	22.937908122350784	27.477542741234423	25.593356829500042	23.991192306914748
106-107	22.844093421635637	27.461263850584682	25.59020295939633	24.10443976838335
108-109	22.78415542669223	27.624560705970357	25.364491901490315	24.2267919658471
110-111	22.631852923784994	28.22099842148798	25.20615838691907	23.94099026780796
112-113	22.60585518047689	28.246073915841468	24.763222000222374	24.384848903459265
114-115	22.633518860423433	28.14188779429403	25.046662533859816	24.17793081142272
116-117	22.85719580570381	27.8823993207181	25.11860477653782	24.14180009704027
118-119	22.546506222165327	27.695204856455625	25.216781991766663	24.541506929612385
120-121	22.682258371529304	27.832776607052317	25.64326731789323	23.841697703525146
122-123	22.514406612639412	28.08518809873141	25.672154178128224	23.72825111050095
124-125	22.580791866407495	27.852602217333743	25.35869129472482	24.20791462153394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	713.0
1	422.5
2	119.5
3	92.5
4	67.5
5	48.5
6	43.0
7	43.0
8	40.0
9	40.0
10	41.0
11	42.5
12	46.0
13	47.5
14	54.0
15	61.0
16	66.5
17	85.5
18	103.5
19	116.5
20	133.5
21	163.0
22	214.5
23	270.0
24	333.5
25	429.0
26	575.0
27	738.0
28	967.5
29	1524.5
30	2306.0
31	2989.0
32	3762.0
33	4494.0
34	5303.0
35	6053.5
36	6555.5
37	7379.5
38	7701.5
39	7376.0
40	7097.0
41	6800.0
42	6960.0
43	7610.5
44	8630.0
45	9821.0
46	11015.0
47	12176.0
48	13083.5
49	13347.0
50	13537.5
51	13632.0
52	12996.0
53	12540.0
54	12097.0
55	11385.0
56	10583.5
57	9173.0
58	7804.5
59	6947.0
60	5922.5
61	4910.0
62	3966.5
63	3121.5
64	2384.5
65	1733.0
66	1333.0
67	1038.0
68	843.5
69	683.5
70	546.5
71	473.0
72	396.0
73	354.0
74	295.5
75	238.5
76	210.0
77	150.5
78	101.0
79	74.0
80	51.0
81	36.5
82	26.5
83	14.5
84	10.0
85	10.0
86	5.5
87	1.5
88	0.5
89	0.5
90	1.5
91	1.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.360819022190538
2	0.30802444019248165
3	0.29322850638410913
4	0.29692748983620226
5	0.32349291644668926
6	0.3369437653633915
7	0.34501427471341284
8	0.33997020636964953
9	0.354766140178022
10-11	0.3458549527707067
12-13	0.3482088513311296
14-15	0.33980207075819074
16-17	0.2843173189767939
18-19	0.2799457930788657
20-21	0.26094646898402374
22-23	0.25775189236630697
24-25	0.2453098571183574
26-27	0.2474956200673215
28-29	0.23639866971104212
30-31	0.2358942628766658
32-33	0.23454917798499558
34-35	0.24278782294647572
36-37	0.23925697510584135
38-39	0.22093019345683454
40-41	0.24362850100376962
42-43	0.2288325671953971
44-45	0.21689493878182386
46-47	0.22631053302351545
48-49	0.22261154957142235
50-51	0.21891256611932922
52-53	0.2575837567548482
54-55	0.2451417215068986
56-57	0.24648680639856882
58-59	0.2407701956089704
60-61	0.2572474855319306
62-63	0.24009765316313525
64-65	0.24648680639856882
66-67	0.24581426395273373
68-69	0.23572612726520703
70-71	0.23774375460271238
72-73	0.2367349409339597
74-75	0.226646804246433
76-77	0.23269968625894902
78-79	0.24278782294647572
80-81	0.23135460136727878
82-83	0.24951324740482683
84-85	0.22244341395996356
86-87	0.21655866755890632
88-89	0.22311595640579865
90-91	0.21403663338702464
92-93	0.23152273697873757
94-95	0.22126646467975208
96-97	0.2057979884255445
98-99	0.20293968303074528
100-101	0.19083391900571325
102-103	0.2004176488588636
104-105	0.1994088351901109
106-107	0.2002495132474048
108-109	0.2004176488588636
110-111	0.19520544490364147
112-113	0.19570985173801783
114-115	0.19066578339425447
116-117	0.20008137763594605
118-119	0.18057764670672777
120-121	0.186462393107785
122-123	0.18612612188486746
124-125	0.18024137548381022
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	297379.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.80472983111281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.06339930910238	54.91251941264457
2	8.915262145706993	11.376717851607633
3	2.006971383540514	3.8416280071673152
4	0.7218171825648131	1.8422140128401172
5	0.3893579413278389	1.2421439127010516
6	0.25416821221750774	0.9730280467317014
7	0.18447581086856638	0.8239300490991037
8	0.14625078996832036	0.7465193713212402
9	0.11752939042396666	0.6749037902894912
>10	1.0355118512629067	13.484768823181861
>50	0.12536660780540018	5.568746208905154
>100	0.039889375210809146	4.5128805135107575
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	487	0.16376408556084995	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	412	0.13854374384203325	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	397	0.13349967549826988	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	352	0.11836747046697985	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	325	0.10928814744820582	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	317	0.10659797766486537	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.681356114587782E-4	0.0
18-19	0.0	0.0	0.0	3.362712229175564E-4	0.0
20-21	0.0	0.0	0.0	0.0013450848916702256	0.0
22-23	0.0	0.0	0.0	0.005044068343763346	0.0
24-25	0.0	0.0	0.0	0.01025627229898547	0.0
26-27	0.0	0.0	0.0	0.01681356114587782	0.0
28-29	0.0	0.0	0.0	0.03245017301154419	0.0
30-31	0.0	0.0	0.0	0.05598915861577314	0.0
32-33	0.0	0.0	0.0	0.09348339997108068	0.0
34-35	0.0	0.0	0.0	0.13316340427535234	0.0
36-37	0.0	0.0	0.0	0.1928515463432186	0.0
38-39	0.0	0.0	0.0	0.27187528372884434	0.0
40-41	0.0	0.0	0.0	0.3631729207509609	0.0
42-43	0.0	0.0	0.0	0.4410197088563752	0.0
44-45	0.0	0.0	0.0	0.5249193789743055	0.0
46-47	0.0	0.0	0.0	0.622438033620397	0.0
48-49	0.0	0.0	0.0	0.714744484311266	0.0
50-51	0.0	0.0	0.0	0.7981397475948201	0.0
52-53	0.0	0.0	0.0	0.8832163669929618	0.0
54-55	0.0	0.0	0.0	0.9671160371108922	0.0
56-57	0.0	0.0	0.0	1.0510157072288224	0.0
58-59	0.0	0.0	0.0	1.1587906341738994	0.0
60-61	0.0	0.0	0.0	1.2524421697564387	0.0
62-63	0.0	0.0	0.0	1.3486157395108598	0.0
64-65	0.0	0.0	0.0	1.4304977822912848	0.0
66-67	0.0	0.0	0.0	1.5258306739884122	0.0
68-69	0.0	0.0	0.0	1.6401628897803813	0.0
70-71	0.0	0.0	0.0	1.7642469710369597	0.0
72-73	0.0	0.0	0.0	1.8684910501414023	0.0
74-75	0.0	0.0	0.0	1.9708856375197978	0.0
76-77	0.0	0.0	0.0	2.078324293241957	0.0
78-79	0.0	0.0	0.0	2.20593922233917	0.0
80-81	0.0	0.0	0.0	2.3312002528759597	0.0
82-83	0.0	0.0	0.0	2.473947387004462	0.0
84-85	0.0	0.0	0.0	2.5973589258152057	0.0
86-87	0.0	0.0	0.0	2.725646397358253	0.0
88-89	0.0	0.0	0.0	2.8764640408367774	0.0
90-91	0.0	0.0	0.0	3.0395555839517923	0.0
92-93	0.0	0.0	0.0	3.2038240763470185	0.0
94-95	0.0	0.0	0.0	3.355818669105754	0.0
96-97	0.0	0.0	0.0	3.505123092081149	0.0
98-99	0.0	0.0	0.0	3.667878363973246	0.0
100-101	0.0	0.0	0.0	3.856862791252913	0.0
102-103	0.0	0.0	0.0	4.051395693710719	0.0
104-105	0.0	0.0	0.0	4.2390350360987155	0.0
106-107	0.0	0.0	0.0	4.411710309066882	0.0
108-109	0.0	0.0	0.0	4.6131367715944975	0.0
110-111	0.0	0.0	0.0	4.830031710376321	0.0
112-113	0.0	0.0	0.0	5.052979531170661	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACT	60	0.0012966405	39.730564	4
AAAACGC	65	0.001922931	36.668182	5
ATGGGTG	125	4.8229456E-5	28.601181	5
TTGTACG	85	0.0071513955	28.040373	7
ACGGTTA	55	7.7464443E-4	27.045664	46-47
GGTATCA	1885	0.0	26.887072	1
GTACAAA	160	8.570816E-6	26.08639	1
GGTAACA	115	9.907389E-4	25.924362	1
GTAGGAC	165	1.0924898E-5	25.283089	3
TCTAACG	120	0.0012748583	24.827414	2
TAGGACT	195	1.4383859E-6	24.44958	4
TAGTACT	245	1.5832484E-8	24.324835	4
ATTTAGG	300	2.1645974E-10	23.850414	1
CTAACGC	125	0.001615469	23.838339	3
CGCCTAC	150	1.666125E-4	23.834316	7
GTATCAA	2175	0.0	23.286402	2
ATATTGG	205	2.2289223E-6	23.24907	6
TTTAGGG	310	3.274181E-10	23.065468	2
CTGTGCG	240	3.495661E-7	22.344671	9
CTCGTCG	215	3.3658162E-6	22.17146	9
>>END_MODULE