FastQCFastQC Report
Sun 1 Jan 2017
ERR1142593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297379
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17710.5955363357869924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16750.563254298386907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8630.29020206537785115No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5460.18360408771298578No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.15165832153581793No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3740.12576543737116608No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC3630.12206645391907296No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC3050.1025627229898547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA1200.054.54021
GTCAACG905.143531E-639.6656041
TAATAGT758.191518E-539.652264
CATGGGT1703.255991E-1034.987294
GGTATCA19250.032.7627561
ATGGGTA1055.9078104E-428.3230445
GTATTAA850.0072028527.9992491
GTATCAA24600.027.8142931
GAGTACT15550.023.90610512-13
ATCAACG28250.023.5808143
CAACGCA28700.023.211085
CATGGGG12400.023.0238934
TATCAAC29200.023.0173242
CTTGTAC1300.002052936522.8763053
ACATGGG25100.022.5117443
AACGCAG30800.022.207846
ATGGGGG9450.022.0290345
AGTACTT15950.021.81495912-13
TACATAT1653.2154153E-421.6285063
TCAACGC31400.021.5940654
Produced by FastQC (version 0.11.5)