##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142593_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297379
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.362063225715332	27.0	14.0	33.0	14.0	33.0
2	29.003931010595906	33.0	27.0	33.0	27.0	33.0
3	31.288234878723785	33.0	33.0	33.0	27.0	33.0
4	31.031589318680876	33.0	33.0	33.0	27.0	33.0
5	31.86968817569499	33.0	33.0	33.0	27.0	33.0
6	34.894965683521704	37.0	37.0	37.0	27.0	37.0
7	35.634076380645574	37.0	37.0	37.0	33.0	37.0
8	35.46170711449027	37.0	37.0	37.0	33.0	37.0
9	35.91470816701919	37.0	37.0	37.0	33.0	37.0
10-11	35.994219497678046	37.0	37.0	37.0	33.0	37.0
12-13	36.08615436866759	37.0	37.0	37.0	37.0	37.0
14-15	36.030832708429315	37.0	37.0	37.0	35.0	37.0
16-17	36.04497963877745	37.0	37.0	37.0	35.0	37.0
18-19	36.055612534846105	37.0	37.0	37.0	37.0	37.0
20-21	36.07753573722422	37.0	37.0	37.0	37.0	37.0
22-23	36.089195941878884	37.0	37.0	37.0	37.0	37.0
24-25	36.082041771611316	37.0	37.0	37.0	37.0	37.0
26-27	36.0258054536467	37.0	37.0	37.0	37.0	37.0
28-29	35.99341412809916	37.0	37.0	37.0	37.0	37.0
30-31	35.95211497785654	37.0	37.0	37.0	35.0	37.0
32-33	35.8868396894199	37.0	37.0	37.0	33.0	37.0
34-35	35.75361407496831	37.0	37.0	37.0	33.0	37.0
36-37	35.652571297906036	37.0	37.0	37.0	33.0	37.0
38-39	35.617101409312696	37.0	37.0	37.0	33.0	37.0
40-41	35.50098863739538	37.0	37.0	37.0	33.0	37.0
42-43	35.44152075297852	37.0	37.0	37.0	33.0	37.0
44-45	35.3204513432354	37.0	37.0	37.0	33.0	37.0
46-47	35.137869520040084	37.0	37.0	37.0	33.0	37.0
48-49	34.93560910487963	37.0	37.0	37.0	27.0	37.0
50-51	34.82542647597846	37.0	37.0	37.0	27.0	37.0
52-53	34.71630142007338	37.0	37.0	37.0	27.0	37.0
54-55	34.67299977469828	37.0	37.0	37.0	27.0	37.0
56-57	34.6473221041163	37.0	37.0	37.0	27.0	37.0
58-59	34.60341853325218	37.0	37.0	37.0	27.0	37.0
60-61	34.55857508432001	37.0	37.0	37.0	27.0	37.0
62-63	34.53798351598465	37.0	37.0	37.0	27.0	37.0
64-65	34.50787379068461	37.0	37.0	37.0	27.0	37.0
66-67	34.49946028468722	37.0	37.0	37.0	27.0	37.0
68-69	34.47438117688203	37.0	37.0	37.0	27.0	37.0
70-71	34.37860440717065	37.0	37.0	37.0	27.0	37.0
72-73	34.3818494244718	37.0	37.0	37.0	27.0	37.0
74-75	34.301848819183604	37.0	37.0	37.0	27.0	37.0
76-77	34.18988059008875	37.0	37.0	37.0	27.0	37.0
78-79	34.19847904525874	37.0	37.0	37.0	27.0	37.0
80-81	34.19027570877567	37.0	37.0	37.0	27.0	37.0
82-83	34.11709804660046	37.0	37.0	37.0	27.0	37.0
84-85	34.03758167187327	37.0	37.0	37.0	27.0	37.0
86-87	33.90106396214931	37.0	37.0	37.0	24.5	37.0
88-89	33.81437324088117	37.0	37.0	37.0	22.0	37.0
90-91	33.75924325523995	37.0	37.0	37.0	22.0	37.0
92-93	33.582684049647085	37.0	35.0	37.0	18.0	37.0
94-95	33.64194680861796	37.0	33.0	37.0	22.0	37.0
96-97	33.632166360099404	37.0	33.0	37.0	22.0	37.0
98-99	33.6046543299964	37.0	33.0	37.0	22.0	37.0
100-101	33.4630252976841	37.0	33.0	37.0	14.0	37.0
102-103	33.391813140806846	37.0	33.0	37.0	14.0	37.0
104-105	33.25222863752988	37.0	33.0	37.0	14.0	37.0
106-107	33.213960972361875	37.0	33.0	37.0	14.0	37.0
108-109	33.07573500482549	37.0	33.0	37.0	14.0	37.0
110-111	33.005077695466056	37.0	33.0	37.0	14.0	37.0
112-113	32.89922119584772	37.0	33.0	37.0	14.0	37.0
114-115	32.86918713157284	37.0	33.0	37.0	14.0	37.0
116-117	32.71107240255701	37.0	33.0	37.0	14.0	37.0
118-119	32.5757013777032	37.0	33.0	37.0	14.0	37.0
120-121	32.48747557830244	37.0	33.0	37.0	14.0	37.0
122-123	32.41770266225927	37.0	33.0	37.0	14.0	37.0
124-125	31.03397684436359	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	12.0
15	154.0
16	50.0
17	25.0
18	65.0
19	296.0
20	926.0
21	2441.0
22	3709.0
23	5012.0
24	4078.0
25	2805.0
26	2521.0
27	2990.0
28	3738.0
29	4613.0
30	6073.0
31	8225.0
32	11515.0
33	17056.0
34	28004.0
35	57726.0
36	135345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.40112213285972	15.670168286291883	12.41645391216014	21.512255668688255
2	18.07558704548741	20.887150740301095	38.11701566015085	22.920246554060643
3	20.40265251632602	27.553820390210436	28.606689129659895	23.436837963803644
4	14.488776796973518	18.6021017234132	35.153930222782684	31.7551912568306
5	15.41469943370593	35.331840253958006	33.11239793928144	16.141062373054627
6	28.805671011393137	35.3452914195015	18.96177178752539	16.887265781579973
7	25.192094936091653	32.767613045978365	23.289136085601204	18.75115593232878
8	25.718023128734714	31.856318031871787	21.298746717152188	21.126912122241315
9	26.76987951402083	15.858214601569042	21.66057455301148	35.711331331398654
10-11	25.230093584281338	25.29717969325339	26.70346594749461	22.76926077497066
12-13	26.718093745691522	24.531826389893034	26.573833391059893	22.176246473355548
14-15	24.579913174770244	24.34738162412275	26.229155387569396	24.843549813537606
16-17	22.816506881790577	26.377787268098956	27.216279562443884	23.589426287666583
18-19	22.606337367467106	24.90071592143359	30.173448696780873	22.319498014318427
20-21	23.969701809988987	24.015603063446292	29.964102865885955	22.050592260678766
22-23	24.101062953335642	24.031959217026085	29.601115075375194	22.265862754263075
24-25	23.29518896761372	24.643972842736037	29.82994764257059	22.230890547079653
26-27	22.89317672061578	24.808241335131264	29.641972028959678	22.65660991529328
28-29	22.457053600402183	24.684869180987736	30.441712778245762	22.41636444036432
30-31	23.053151208226566	24.32274747963871	30.184983421772966	22.43911789036176
32-33	22.640919370160823	24.77759749812948	29.720473135997178	22.861009995712518
34-35	22.759340773894593	24.524260287377388	30.134609370533898	22.58178956819412
36-37	23.0432567578701	24.594157891285597	29.675107734161017	22.687477616683285
38-39	23.006500122738995	24.41783044532398	29.94024460368755	22.635424828249473
40-41	22.572677595628416	24.484909625893234	29.938461538461535	23.003951240016814
42-43	22.917528373266077	24.646321984026905	29.40159730979403	23.034552332912988
44-45	23.070727926316252	24.81025896246877	29.13790146580626	22.98111164540872
46-47	23.12856657665807	24.674237252798616	28.746649897941683	23.45054627260163
48-49	23.348992363280527	24.680794541645508	28.697218028172806	23.27299506690116
50-51	23.08300182595274	25.06162170159964	28.26662272722687	23.588753745220746
52-53	22.64181398148491	25.240349856580323	27.946492523009358	24.17134363892541
54-55	22.752110942601863	25.362752581722315	27.689749444311808	24.195387031364017
56-57	23.310825579479385	24.94779389264205	27.467642301574756	24.273738226303806
58-59	23.06669267164124	25.032702376428734	28.09495626792746	23.80564868400257
60-61	23.032393006903646	25.154600694736345	28.168767801357863	23.644238497002142
62-63	22.87031027745739	25.091381704827846	28.104371862169153	23.933936155545616
64-65	22.56396719337949	25.107018316693512	28.081169147787836	24.247845342139158
66-67	23.225412688858327	24.635061655328723	28.137998984460904	24.001526671352046
68-69	22.757827553391465	24.5254372366576	28.45947427357009	24.25726093638085
70-71	22.64534482932554	24.71946573228103	28.691333281771747	23.943856156621685
72-73	23.25349133597194	24.412954512591675	28.967243820175604	23.36631033126078
74-75	23.12210700242124	24.692801725660964	28.902802011700707	23.282289260217087
76-77	22.82747907631151	24.741367558479457	28.76840973235518	23.662743632853854
78-79	22.430542945342292	25.00790240031206	28.84191164107634	23.719643013269305
80-81	22.28486207835792	25.072886787567377	29.025586877351795	23.616664256722903
82-83	22.721846532539285	24.612867754616165	29.25895910605658	23.40632660678797
84-85	22.889036213911446	25.01472315422125	28.473738940807873	23.622501691059426
86-87	22.410724059245037	25.328180126472493	28.668333880651055	23.59276193363141
88-89	23.06005037189282	24.577440467666804	28.594725271022686	23.76778388941769
90-91	22.909890065509718	24.619402520432875	28.65465290620483	23.816054507852584
92-93	22.768468212131264	24.84523186178031	28.616237311706485	23.770062614381946
94-95	21.897046856189764	24.84094317669767	28.97658871873508	24.285421248377485
96-97	22.26552648304016	24.90592812538881	28.360610534032325	24.467934857538694
98-99	22.26804851721204	25.3260990184243	27.90731692553946	24.498535538824193
100-101	22.579771940856617	25.25497765477724	27.685377918413877	24.47987248595227
102-103	22.51184515382727	25.292808167355464	27.778693182773495	24.416653496043768
104-105	22.594980126303895	25.589821708398063	27.592155438532778	24.22304272676526
106-107	22.5115467325311	25.306469701071194	27.58824192064995	24.59374164574776
108-109	22.512837240270766	25.4942379538431	27.373502321968413	24.61942248391772
110-111	22.172921041702747	26.212217762884677	27.106028176213144	24.50883301919944
112-113	22.321683777267392	26.03210045093971	26.79728561868861	24.848930153104288
114-115	22.47455683581698	25.89191888451922	26.838019560929926	24.79550471873387
116-117	22.455931508046998	25.83277848394972	26.964166817989227	24.747123190014054
118-119	22.238624785206756	25.66169769889602	27.229226004526208	24.870451511371012
120-121	22.266199025485996	25.639335662572005	27.683696562299286	24.41076874964271
122-123	22.128832230924175	26.212678097646435	27.580629432475057	24.077860238954333
124-125	22.24503281194042	25.767167210589943	27.49965111567323	24.48814886179641
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	39.5
2	81.0
3	148.0
4	148.0
5	141.5
6	205.0
7	370.0
8	596.0
9	764.5
10	761.0
11	673.0
12	646.0
13	667.0
14	688.5
15	688.5
16	686.5
17	728.0
18	759.0
19	725.5
20	702.5
21	696.0
22	667.5
23	656.5
24	677.0
25	711.5
26	788.5
27	867.5
28	969.0
29	1347.5
30	2059.5
31	2678.5
32	3366.0
33	4059.5
34	4787.0
35	5432.5
36	5966.5
37	6791.5
38	7126.5
39	6896.5
40	6588.0
41	6326.5
42	6361.0
43	6988.0
44	8151.0
45	9288.5
46	10400.5
47	11472.5
48	12403.5
49	13038.5
50	13601.5
51	13518.5
52	12570.5
53	12082.0
54	11835.0
55	11284.5
56	10723.0
57	9408.0
58	7972.5
59	7127.0
60	6145.0
61	5003.5
62	3945.0
63	3211.5
64	2424.0
65	1717.0
66	1273.0
67	963.5
68	781.5
69	699.5
70	626.5
71	528.0
72	421.0
73	386.5
74	343.5
75	254.0
76	195.5
77	146.0
78	113.5
79	85.5
80	57.0
81	35.5
82	27.5
83	25.0
84	15.5
85	8.5
86	7.5
87	5.0
88	3.5
89	1.5
90	0.0
91	0.5
92	0.5
93	0.0
94	1.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02959186761674496
2	0.0
3	3.362712229175564E-4
4	0.0013450848916702256
5	0.0023538985604228944
6	0.0023538985604228944
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.044068343763346E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	8.406780572938909E-4
30-31	3.362712229175564E-4
32-33	5.044068343763346E-4
34-35	0.0
36-37	0.0011769492802114472
38-39	0.0
40-41	0.0013450848916702256
42-43	0.0013450848916702256
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07313899098456851
76-77	0.09667797658879747
78-79	3.362712229175564E-4
80-81	0.0
82-83	0.0
84-85	0.07666983882520285
86-87	0.1602332377202156
88-89	0.1972230722411468
90-91	0.0832271276720952
92-93	0.13534916722431645
94-95	3.362712229175564E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	3.362712229175564E-4
106-107	1.681356114587782E-4
108-109	6.725424458351128E-4
110-111	1.681356114587782E-4
112-113	0.0
114-115	1.681356114587782E-4
116-117	3.362712229175564E-4
118-119	0.0
120-121	0.0
122-123	0.0
124-125	8.406780572938909E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	297379.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.85233862679801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.25268377452153	50.42720159489193
2	9.950828219539263	11.911606804259229
3	2.2628943868418383	4.063185633538145
4	0.8735940572530082	2.0914658934826167
5	0.4595683221852586	1.3753119420790754
6	0.3162708130869985	1.1357728681593464
7	0.20867188768944728	0.8742650338717375
8	0.18190843691740027	0.8710116292361412
9	0.14228156214291318	0.7664295813954701
>10	1.16432434059168	14.248546422462754
>50	0.1385879048610573	5.813875381306666
>100	0.04599684773405781	4.685495164628488
>500	0.0011947233177677353	0.5037667063686792
>1k	0.0011947233177677353	1.2320653443197078
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1771	0.5955363357869924	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1675	0.563254298386907	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	863	0.29020206537785115	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	546	0.18360408771298578	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.15165832153581793	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	374	0.12576543737116608	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	363	0.12206645391907296	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	305	0.1025627229898547	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.681356114587782E-4	0.0
18-19	0.0	0.0	0.0	3.362712229175564E-4	0.0
20-21	0.0	0.0	0.0	0.0013450848916702256	0.0
22-23	0.0	0.0	0.0	0.005044068343763346	0.0
24-25	0.0	0.0	0.0	0.01025627229898547	0.0
26-27	0.0	0.0	0.0	0.017149832368795376	0.0
28-29	0.0	0.0	0.0	0.032113901788626636	0.0
30-31	0.0	0.0	0.0	0.05666170106160825	0.0
32-33	0.0	0.0	0.0	0.09398780680545701	0.0
34-35	0.0	0.0	0.0	0.13232272621805843	0.0
36-37	0.0	0.0	0.0	0.19150646145154837	0.0
38-39	0.0	0.0	0.0	0.2703620632257153	0.0
40-41	0.0	0.0	0.0	0.36115529341345554	0.0
42-43	0.0	0.0	0.0	0.4390020815188699	0.0
44-45	0.0	0.0	0.0	0.523742429694094	0.0
46-47	0.0	0.0	0.0	0.6215973555631029	0.0
48-49	0.0	0.0	0.0	0.7140719418654309	0.0
50-51	0.0	0.0	0.0	0.7984760188177376	0.0
52-53	0.0	0.0	0.0	0.8850658587190083	0.0
54-55	0.0	0.0	0.0	0.9703106137286089	0.0
56-57	0.0	0.0	0.0	1.0555553687382095	0.0
58-59	0.0	0.0	0.0	1.165179787409333	0.0
60-61	0.0	0.0	0.0	1.2601764078835425	0.0
62-63	0.0	0.0	0.0	1.3573587913067162	0.0
64-65	0.0	0.0	0.0	1.4400815121444352	0.0
66-67	0.0	0.0	0.0	1.5367594887332325	0.0
68-69	0.0	0.0	0.0	1.6522686538054132	0.0
70-71	0.0	0.0	0.0	1.776016463839074	0.0
72-73	0.0	0.0	0.0	1.8802605429435166	0.0
74-75	0.0	0.0	0.0	1.9834958083792062	0.0
76-77	0.0	0.0	0.0	2.0905981928784483	0.0
78-79	0.0	0.0	0.0	2.2175405795298255	0.0
80-81	0.0	0.0	0.0	2.3412883895634864	0.0
82-83	0.0	0.0	0.0	2.4838673880805304	0.0
84-85	0.0	0.0	0.0	2.60643824883398	0.0
86-87	0.0	0.0	0.0	2.7343894491541096	0.0
88-89	0.0	0.0	0.0	2.885543363855551	0.0
90-91	0.0	0.0	0.0	3.047962364524731	0.0
92-93	0.0	0.0	0.0	3.213071534977251	0.0
94-95	0.0	0.0	0.0	3.364897992124528	0.0
96-97	0.0	0.0	0.0	3.515211228768676	1.681356114587782E-4
98-99	0.0	0.0	0.0	3.678639043106608	3.362712229175564E-4
100-101	0.0	0.0	0.0	3.868127877220651	3.362712229175564E-4
102-103	0.0	0.0	0.0	4.062660779678458	3.362712229175564E-4
104-105	0.0	0.0	0.0	4.251813342569583	3.362712229175564E-4
106-107	0.0	0.0	0.0	4.426001836040877	3.362712229175564E-4
108-109	0.0	0.0	0.0	4.6294459259059995	3.362712229175564E-4
110-111	0.0	0.0	0.0	4.847013407133657	3.362712229175564E-4
112-113	0.0	0.0	0.0	5.074500889437385	3.362712229175564E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAGA	120	0.0	54.5402	1
GTCAACG	90	5.143531E-6	39.665604	1
TAATAGT	75	8.191518E-5	39.65226	4
CATGGGT	170	3.255991E-10	34.98729	4
GGTATCA	1925	0.0	32.762756	1
ATGGGTA	105	5.9078104E-4	28.323044	5
GTATTAA	85	0.00720285	27.999249	1
GTATCAA	2460	0.0	27.814293	1
GAGTACT	1555	0.0	23.906105	12-13
ATCAACG	2825	0.0	23.580814	3
CAACGCA	2870	0.0	23.21108	5
CATGGGG	1240	0.0	23.023893	4
TATCAAC	2920	0.0	23.017324	2
CTTGTAC	130	0.0020529365	22.876305	3
ACATGGG	2510	0.0	22.511744	3
AACGCAG	3080	0.0	22.20784	6
ATGGGGG	945	0.0	22.029034	5
AGTACTT	1595	0.0	21.814959	12-13
TACATAT	165	3.2154153E-4	21.628506	3
TCAACGC	3140	0.0	21.594065	4
>>END_MODULE