FastQCFastQC Report
Sun 1 Jan 2017
ERR1142592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392685
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15060.38351350318957944No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6630.16883761793804195No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.040.7990571
GTATCAA20300.032.8552471
AACGCAG23700.027.6393076
TATCAAC24600.026.8667552
TCAACGC24050.026.2466344
ATCAACG24150.026.1379493
CAACGCA24500.026.0076125
ACGCAGA26550.023.9994937
CGCAGAG27000.023.8200578
GCAGAGT28600.022.279259
TTCGCTA700.003141725721.2651910-11
CAGAGTA28300.021.14496410-11
CATGGGG20900.019.6600954
GTACATG36100.019.3001841
AGAGTAC27700.018.70108410-11
GAGTACA31400.018.5833412-13
TACATGG37250.018.5421052
ACATGGG37300.018.5196153
ATGGGGA6800.018.3904865
ATGGGGG11500.018.1239575
Produced by FastQC (version 0.11.5)