##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142592_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392685
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0691037345455	33.0	33.0	33.0	27.0	33.0
2	31.156010542801482	33.0	33.0	33.0	27.0	33.0
3	31.26798833670754	33.0	33.0	33.0	27.0	33.0
4	31.15728128143423	33.0	33.0	33.0	27.0	33.0
5	31.2508881164292	33.0	33.0	33.0	27.0	33.0
6	34.38411704037588	37.0	37.0	37.0	27.0	37.0
7	34.53974814418682	37.0	37.0	37.0	27.0	37.0
8	34.50325579026446	37.0	37.0	37.0	27.0	37.0
9	34.36858805403823	37.0	37.0	37.0	27.0	37.0
10-11	34.47241427607369	37.0	37.0	37.0	27.0	37.0
12-13	34.48171944433834	37.0	37.0	37.0	27.0	37.0
14-15	34.430604683142974	37.0	37.0	37.0	27.0	37.0
16-17	34.4542826947808	37.0	37.0	37.0	27.0	37.0
18-19	34.46400168073647	37.0	37.0	37.0	27.0	37.0
20-21	34.52455403185759	37.0	37.0	37.0	27.0	37.0
22-23	34.59319429058915	37.0	37.0	37.0	27.0	37.0
24-25	34.658322828730405	37.0	37.0	37.0	27.0	37.0
26-27	34.660444121878854	37.0	37.0	37.0	27.0	37.0
28-29	34.721650941594405	37.0	37.0	37.0	27.0	37.0
30-31	34.791116289137605	37.0	37.0	37.0	27.0	37.0
32-33	34.80583673937125	37.0	37.0	37.0	27.0	37.0
34-35	34.81443778091855	37.0	37.0	37.0	27.0	37.0
36-37	34.7555317875651	37.0	37.0	37.0	27.0	37.0
38-39	34.73783184995607	37.0	37.0	37.0	27.0	37.0
40-41	34.73098794198913	37.0	37.0	37.0	27.0	37.0
42-43	34.71093242675427	37.0	37.0	37.0	27.0	37.0
44-45	34.73322765066147	37.0	37.0	37.0	27.0	37.0
46-47	34.712978596075736	37.0	37.0	37.0	27.0	37.0
48-49	34.68894788443663	37.0	37.0	37.0	27.0	37.0
50-51	34.65033169079542	37.0	37.0	37.0	27.0	37.0
52-53	34.61294803723086	37.0	37.0	37.0	27.0	37.0
54-55	34.57399314972561	37.0	37.0	37.0	27.0	37.0
56-57	34.497978023097396	37.0	37.0	37.0	27.0	37.0
58-59	34.42945745317493	37.0	37.0	37.0	27.0	37.0
60-61	34.39606682200746	37.0	37.0	37.0	27.0	37.0
62-63	34.42290512751951	37.0	37.0	37.0	27.0	37.0
64-65	34.38469511185811	37.0	37.0	37.0	27.0	37.0
66-67	34.342210677769714	37.0	37.0	37.0	27.0	37.0
68-69	34.311878477660215	37.0	37.0	37.0	27.0	37.0
70-71	34.28487973821257	37.0	37.0	37.0	27.0	37.0
72-73	34.24779276010034	37.0	37.0	37.0	27.0	37.0
74-75	34.161513681449506	37.0	37.0	37.0	27.0	37.0
76-77	34.097662248367016	37.0	37.0	37.0	27.0	37.0
78-79	34.038945974508835	37.0	37.0	37.0	27.0	37.0
80-81	33.97254669773483	37.0	37.0	37.0	27.0	37.0
82-83	33.94952315469142	37.0	37.0	37.0	27.0	37.0
84-85	33.91329946394693	37.0	37.0	37.0	27.0	37.0
86-87	33.8596521384825	37.0	37.0	37.0	22.0	37.0
88-89	33.78945337866229	37.0	37.0	37.0	22.0	37.0
90-91	33.69961674115385	37.0	35.0	37.0	22.0	37.0
92-93	33.57285610603919	37.0	33.0	37.0	22.0	37.0
94-95	33.50198505163171	37.0	33.0	37.0	22.0	37.0
96-97	33.40951780689357	37.0	33.0	37.0	18.0	37.0
98-99	33.3684951042184	37.0	33.0	37.0	18.0	37.0
100-101	33.2598151189885	37.0	33.0	37.0	14.0	37.0
102-103	33.18194990896011	37.0	33.0	37.0	14.0	37.0
104-105	33.042735271273415	37.0	33.0	37.0	14.0	37.0
106-107	32.94463373951132	37.0	33.0	37.0	14.0	37.0
108-109	32.830143753899435	37.0	33.0	37.0	14.0	37.0
110-111	32.75861950418275	37.0	33.0	37.0	14.0	37.0
112-113	32.6668411067395	37.0	33.0	37.0	14.0	37.0
114-115	32.48374778766696	37.0	33.0	37.0	14.0	37.0
116-117	32.28887530718005	37.0	33.0	37.0	14.0	37.0
118-119	32.16214523091027	37.0	33.0	37.0	14.0	37.0
120-121	32.08203139921311	37.0	33.0	37.0	14.0	37.0
122-123	31.867789704215845	37.0	30.0	37.0	14.0	37.0
124-125	30.227494047391676	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	662.0
1	67.0
2	29.0
3	11.0
4	12.0
5	17.0
6	16.0
7	19.0
8	13.0
9	9.0
10	19.0
11	11.0
12	12.0
13	9.0
14	23.0
15	163.0
16	257.0
17	469.0
18	1160.0
19	2242.0
20	3404.0
21	3941.0
22	4009.0
23	3877.0
24	4208.0
25	4647.0
26	5662.0
27	6400.0
28	7712.0
29	9114.0
30	10996.0
31	13143.0
32	16735.0
33	22103.0
34	33077.0
35	62778.0
36	175659.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.104944108521984	19.648908493357936	12.13997659049206	24.106170807628025
2	17.413239058133534	23.299649022422717	35.64746933416437	23.63964258527938
3	19.760685507042794	27.88818368728211	27.83761349560065	24.51351731007445
4	14.53875801685231	19.701470426722587	33.14781658105993	32.61195497536518
5	15.271483387066285	38.12927585695965	30.554207264422317	16.04503349155175
6	29.532389357016203	35.99518643617429	18.070537616826012	16.401886589983494
7	26.314552251200276	33.93472656240019	20.989398751561826	18.76132243483771
8	25.64257828468645	34.284553593328425	20.048186983893384	20.024681138091736
9	26.64569593114921	19.034392176603916	19.18387893872065	35.136032953526225
10-11	25.12405077522153	27.802321065380255	24.952219371850823	22.1214087875474
12-13	25.888014881267974	26.64894235425032	24.77051444369635	22.692528320785353
14-15	24.651726071776686	26.75638007176902	23.09102452895991	25.500869327494378
16-17	24.063308029700377	28.699555005075624	23.588561651269544	23.648575313954453
18-19	24.124040153320106	27.81327327189293	25.05739289426173	23.00529368052523
20-21	26.183856101515325	26.215515810705575	24.615934535891643	22.984693551887457
22-23	26.06614319579351	26.00282628315681	24.29033357545244	23.640696945597238
24-25	24.925427428155693	26.537113974459615	24.510603536852315	24.02685506053238
26-27	24.41698683992188	26.696960800582055	24.768517927574894	24.117534431921168
28-29	24.352664896875638	26.7634521135389	24.86841433530733	24.01546865427813
30-31	25.01110356692515	26.402622994338458	24.833191241710615	23.753082197025776
32-33	24.509182057306656	26.812449667470283	24.4863369915218	24.192031283701258
34-35	24.595355248798604	26.84433893026491	24.7541370705775	23.80616875035898
36-37	24.61181888029242	27.10950242876433	24.38553411118457	23.89314457975868
38-39	24.374087609869637	27.00457853956328	24.45473524097308	24.166598609594008
40-41	24.15344556907093	26.87160963406384	24.71875119659974	24.256193600265487
42-43	24.321264947230055	26.998557919320053	24.589645095011424	24.090532038438468
44-45	24.323500062526637	26.9551058741862	24.605125142599015	24.11626892068815
46-47	24.218567547183294	26.71093284396364	24.709383570297337	24.36111603855573
48-49	24.114679771273398	27.133273697334122	24.670417524306348	24.081629007086136
50-51	23.97233965716794	27.340301589104303	24.707490215645013	23.979868538082737
52-53	23.90783732514291	26.95358675643451	24.565905069738587	24.57267084868399
54-55	23.644799571124967	26.682664386140697	24.8259928903752	24.846543152359132
56-57	23.928005248606794	26.65761264975506	24.31603454481866	25.09834755681949
58-59	23.963190105745483	26.716784622551803	24.611702840513857	24.708322431188854
60-61	24.13739401722276	26.560477519269014	24.869859149176264	24.43226931433196
62-63	23.76003839222353	26.586359528878408	24.82680103944903	24.82680103944903
64-65	23.593450440492084	26.379489483586532	24.92373398413667	25.103326091784716
66-67	23.90574023679817	26.210412380081998	24.825005233249772	25.058842149870063
68-69	23.96611731312386	26.12149932293335	24.658244068168678	25.25413929577411
70-71	23.79939221364673	26.250448309442643	24.813815971685987	25.13634350522464
72-73	23.897564181923766	26.456417554226785	24.81203295405959	24.833985309789856
74-75	23.86200489284616	26.125169571448442	25.084578346156743	24.928247189548657
76-77	23.785864428281354	25.79622508586022	25.043871348148983	25.37403913770945
78-79	23.90510506370959	25.92979755627798	25.061105814210244	25.103991565802186
80-81	23.805774043732235	26.126775752640896	25.105769832082814	24.961680371544055
82-83	23.922785994462835	26.095403180616593	25.180966336623552	24.80084448829702
84-85	23.830603944969	26.264147283606697	24.857617934971945	25.047630836452363
86-87	23.471420277279776	26.60877776147304	25.03467592208604	24.885126039161143
88-89	23.603847793184947	25.977708526348298	25.21611295299496	25.202330727471807
90-91	24.03589114753931	25.84512245072432	24.98905835501459	25.129928046721783
92-93	24.012828759291025	26.13687413215715	24.85769929755779	24.992597810994038
94-95	23.460720784640003	26.337019521569687	25.038091178925175	25.164168514865132
96-97	23.375778299479432	26.432708992548736	24.860416454016534	25.331096253955295
98-99	23.213850560414397	26.58628850656103	24.9149331138882	25.28492781913638
100-101	23.42339928814727	26.966078558944723	24.638651817265618	24.97187033564239
102-103	23.60689912976248	26.783565894242088	24.92813938089509	24.681395595100337
104-105	23.58280057717554	26.93192416086918	24.81000029343584	24.675274968519435
106-107	23.47810776811254	26.87402557304598	25.000956884079233	24.646909774762246
108-109	23.376914990252814	26.953264541678152	24.76844055237454	24.9013799156945
110-111	23.09908172143424	27.615078241212053	24.68151119207816	24.604328845275546
112-113	23.10764037289031	27.806150112971935	24.468279190582567	24.61793032355519
114-115	23.144612848947496	27.83377323180587	24.432416158838006	24.589197760408627
116-117	23.14176323420781	27.773688125222467	24.44422897574829	24.640319664821433
118-119	22.896087595412055	27.41132291113923	24.873209110575942	24.819380382872772
120-121	23.22273249138921	27.304630692690395	24.95114172726113	24.521495088659268
122-123	23.13104021880007	27.623381195848513	24.860188388492585	24.38539019685883
124-125	23.023642938689083	27.494690384014387	24.52538729901589	24.95627937828064
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1551.0
1	1036.0
2	413.5
3	277.5
4	220.5
5	168.5
6	149.0
7	147.0
8	135.0
9	136.0
10	131.0
11	113.0
12	107.5
13	107.5
14	115.5
15	130.0
16	131.5
17	137.0
18	172.0
19	206.5
20	243.0
21	283.5
22	346.5
23	449.5
24	566.0
25	723.0
26	950.0
27	1203.0
28	1450.0
29	1896.5
30	2533.0
31	3113.0
32	3806.0
33	4476.0
34	5180.0
35	5801.5
36	6317.0
37	7243.0
38	7937.0
39	8224.5
40	8530.5
41	8885.5
42	9420.5
43	10235.0
44	11167.0
45	12111.0
46	13350.5
47	14562.5
48	15562.5
49	16370.0
50	16820.0
51	16840.0
52	16598.0
53	16657.0
54	16487.0
55	16015.5
56	15526.5
57	14242.0
58	12655.5
59	11216.0
60	9691.5
61	8269.5
62	7000.5
63	5543.0
64	4172.5
65	3338.5
66	2636.5
67	2033.0
68	1626.0
69	1302.0
70	1134.0
71	1009.5
72	870.0
73	808.0
74	627.5
75	470.5
76	396.5
77	294.0
78	211.0
79	145.0
80	94.0
81	65.5
82	49.0
83	37.0
84	21.0
85	10.0
86	7.5
87	5.0
88	3.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3542279435170684
2	0.30737104804105075
3	0.29285559672511047
4	0.2969301093751989
5	0.3165387015037498
6	0.32876223945401534
7	0.3351286654697786
8	0.3292715535352764
9	0.3427683766886945
10-11	0.33436469434788696
12-13	0.33627462215261594
14-15	0.32863491093370006
16-17	0.2820326724983129
18-19	0.2765575461247565
20-21	0.25975018144314144
22-23	0.2558029973133682
24-25	0.24256083120058064
26-27	0.2470173294116149
28-29	0.23555776258324104
30-31	0.23402982033945782
32-33	0.233138520697251
34-35	0.24256083120058064
36-37	0.23479379146134943
38-39	0.21824108382036495
40-41	0.2419241885990043
42-43	0.22664476616117246
44-45	0.21696779861721227
46-47	0.22626278060022664
48-49	0.21989635458446338
50-51	0.21913238346257177
52-53	0.2566942969555751
54-55	0.2451074016068859
56-57	0.24498007308657066
58-59	0.24001426079427532
60-61	0.25312909838674763
62-63	0.23912296115206846
64-65	0.24548938716783172
66-67	0.24421610196467908
68-69	0.23466646294103416
70-71	0.23823166150986158
72-73	0.23619440518481732
74-75	0.22626278060022664
76-77	0.23288386365662045
78-79	0.24103288895679742
80-81	0.23288386365662045
82-83	0.24663534385066913
84-85	0.22142429682824655
86-87	0.21454855673122222
88-89	0.22307956759234504
90-91	0.21225664336554745
92-93	0.2314832499331525
94-95	0.2195143690235176
96-97	0.20499891770757733
98-99	0.200542419496543
100-101	0.1909927804728981
102-103	0.1987598202121293
104-105	0.1977411920496072
106-107	0.20066974801685827
108-109	0.19786852056992246
110-111	0.1925207227166813
112-113	0.19697722092771558
114-115	0.18806422450564703
116-117	0.19723187796834613
118-119	0.1785145854820021
120-121	0.18717292486344017
122-123	0.18564498261965698
124-125	0.18016985624610057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	392685.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.55129532715601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.26268606957467	68.54597024492062
2	8.661470506922385	13.607394555134238
3	2.0793783751693784	4.90013594534495
4	0.7578412057534131	2.3811763345689743
5	0.39222756499490846	1.5404991646683166
6	0.21756377568021615	1.025394983756867
7	0.14104810717240135	0.7755658065284956
8	0.09957933949789079	0.6257668884305561
9	0.07197010068665999	0.5088010170386683
>10	0.2943554857873786	4.0723448768161505
>50	0.01668773041131545	0.8753517186075649
>100	0.004450061442449045	0.5097722154434654
>500	3.7083845353742047E-4	0.19313084505086717
>1k	3.7083845353742047E-4	0.4386954036902051
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1506	0.38351350318957944	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	663	0.16883761793804195	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.273285203152654E-4	0.0
14-15	0.0	0.0	0.0	2.546570406305308E-4	0.0
16-17	0.0	0.0	0.0	3.8198556094579627E-4	0.0
18-19	0.0	0.0	0.0	5.093140812610617E-4	1.273285203152654E-4
20-21	0.0	0.0	0.0	0.0010186281625221233	2.546570406305308E-4
22-23	0.0	0.0	0.0	0.0025465704063053086	3.8198556094579627E-4
24-25	0.0	0.0	0.0	0.00751238269860066	5.093140812610617E-4
26-27	0.0	0.0	0.0	0.012350866470580747	5.093140812610617E-4
28-29	0.0	0.0	0.0	0.025975018144314146	5.093140812610617E-4
30-31	0.0	0.0	0.0	0.051568050727682495	5.093140812610617E-4
32-33	1.273285203152654E-4	0.0	0.0	0.09727898952086278	5.093140812610617E-4
34-35	2.546570406305308E-4	0.0	0.0	0.14400855647656519	5.093140812610617E-4
36-37	2.546570406305308E-4	0.0	0.0	0.20703617403262156	5.093140812610617E-4
38-39	2.546570406305308E-4	0.0	0.0	0.29540216713141576	5.093140812610617E-4
40-41	2.546570406305308E-4	0.0	0.0	0.3874606873193527	5.093140812610617E-4
42-43	2.546570406305308E-4	0.0	0.0	0.4770999656212995	5.093140812610617E-4
44-45	2.546570406305308E-4	0.0	0.0	0.5590995327043304	5.093140812610617E-4
46-47	2.546570406305308E-4	0.0	0.0	0.6595617352330749	5.093140812610617E-4
48-49	2.546570406305308E-4	0.0	0.0	0.7629524937290704	5.093140812610617E-4
50-51	2.546570406305308E-4	0.0	0.0	0.8517004723888104	5.093140812610617E-4
52-53	2.546570406305308E-4	0.0	0.0	0.9427403644142252	6.366426015763271E-4
54-55	2.546570406305308E-4	0.0	0.0	1.0402740109757185	7.639711218915925E-4
56-57	2.546570406305308E-4	0.0	0.0	1.1334784878464927	7.639711218915925E-4
58-59	2.546570406305308E-4	0.0	0.0	1.2354686326190203	7.639711218915925E-4
60-61	2.546570406305308E-4	0.0	0.0	1.3375861059118632	7.639711218915925E-4
62-63	2.546570406305308E-4	0.0	0.0	1.4337191387498887	7.639711218915925E-4
64-65	2.546570406305308E-4	0.0	0.0	1.5191565758814316	7.639711218915925E-4
66-67	2.546570406305308E-4	0.0	0.0	1.6145256375975654	7.639711218915925E-4
68-69	2.546570406305308E-4	0.0	0.0	1.7293759629219347	7.639711218915925E-4
70-71	2.546570406305308E-4	0.0	0.0	1.8558131835949934	0.0010186281625221233
72-73	2.546570406305308E-4	0.0	0.0	1.966461667748959	0.0010186281625221233
74-75	2.546570406305308E-4	0.0	0.0	2.083858563479634	0.0010186281625221233
76-77	2.546570406305308E-4	0.0	0.0	2.2059666144619734	0.0010186281625221233
78-79	2.546570406305308E-4	0.0	0.0	2.3404255319148937	0.0010186281625221233
80-81	2.546570406305308E-4	0.0	0.0	2.4846887454320896	0.0010186281625221233
82-83	2.546570406305308E-4	0.0	0.0	2.6371009842494617	0.0010186281625221233
84-85	2.546570406305308E-4	0.0	0.0	2.769522645377338	0.0010186281625221233
86-87	2.546570406305308E-4	0.0	0.0	2.908820046602238	0.0010186281625221233
88-89	2.546570406305308E-4	0.0	0.0	3.070654595922941	0.0010186281625221233
90-91	2.546570406305308E-4	0.0	0.0	3.2445853546735934	0.0010186281625221233
92-93	2.546570406305308E-4	0.0	0.0	3.419152756025822	0.0010186281625221233
94-95	2.546570406305308E-4	0.0	0.0	3.586335103199766	0.0010186281625221233
96-97	2.546570406305308E-4	0.0	0.0	3.7484243095610985	0.0010186281625221233
98-99	2.546570406305308E-4	0.0	0.0	3.919044526783554	0.0010186281625221233
100-101	2.546570406305308E-4	0.0	0.0	4.108127379451723	0.0010186281625221233
102-103	2.546570406305308E-4	0.0	0.0	4.323185250264206	0.0010186281625221233
104-105	2.546570406305308E-4	0.0	0.0	4.538370449597005	0.0010186281625221233
106-107	2.546570406305308E-4	0.0	0.0	4.75928543234399	0.0010186281625221233
108-109	2.546570406305308E-4	0.0	0.0	4.980964386212868	0.0010186281625221233
110-111	2.546570406305308E-4	0.0	0.0	5.20277066860206	0.001527942243783185
112-113	2.546570406305308E-4	0.0	0.0	5.428396806600711	0.0017825992844137158
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1445	0.0	40.799057	1
GTATCAA	2030	0.0	32.855247	1
AACGCAG	2370	0.0	27.639307	6
TATCAAC	2460	0.0	26.866755	2
TCAACGC	2405	0.0	26.246634	4
ATCAACG	2415	0.0	26.137949	3
CAACGCA	2450	0.0	26.007612	5
ACGCAGA	2655	0.0	23.999493	7
CGCAGAG	2700	0.0	23.820057	8
GCAGAGT	2860	0.0	22.27925	9
TTCGCTA	70	0.0031417257	21.26519	10-11
CAGAGTA	2830	0.0	21.144964	10-11
CATGGGG	2090	0.0	19.660095	4
GTACATG	3610	0.0	19.300184	1
AGAGTAC	2770	0.0	18.701084	10-11
GAGTACA	3140	0.0	18.58334	12-13
TACATGG	3725	0.0	18.542105	2
ACATGGG	3730	0.0	18.519615	3
ATGGGGA	680	0.0	18.390486	5
ATGGGGG	1150	0.0	18.123957	5
>>END_MODULE