FastQCFastQC Report
Sun 1 Jan 2017
ERR1142592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392685
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.7260272228376434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27130.6908845512306302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.3544826005576989No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6780.1726574735474999No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.14719176948444684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.053.5524941
GTATCAA26950.045.0259481
CCGTCGA650.001941311436.6001439
TATCAAC34050.035.807412
ATCAACG34750.035.086113
TCAACGC35450.034.561074
CAACGCA35650.034.2003445
GTCAACG1051.48413965E-533.9901771
GTCTAGA1251.278062E-633.310371
GCGCAGA2005.2750693E-1132.715541
AACGCAG37750.032.6129076
GTACTAG1152.7648293E-531.0345081
ACGCAGA41150.029.9182827
TAGCCCG800.005368873529.7376165
CGCAGAG42150.029.2084778
GTGGTAT8750.027.8719461
GCAGAGT44800.027.4807459
GTATTCA1755.698712E-727.192141
TGGTATC9850.024.756192
CAGAGTA44450.024.01755710-11
Produced by FastQC (version 0.11.5)