##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142592_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392685
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.337255051759044	27.0	14.0	33.0	14.0	33.0
2	28.767340234539134	33.0	27.0	33.0	27.0	33.0
3	31.17962743674956	33.0	33.0	33.0	27.0	33.0
4	30.868031628404445	33.0	33.0	33.0	27.0	33.0
5	31.797226784827533	33.0	33.0	33.0	27.0	33.0
6	34.7451137680329	37.0	37.0	37.0	27.0	37.0
7	35.52484816073952	37.0	37.0	37.0	33.0	37.0
8	35.33226632033309	37.0	37.0	37.0	33.0	37.0
9	35.82756917121866	37.0	37.0	37.0	33.0	37.0
10-11	35.90813501916294	37.0	37.0	37.0	33.0	37.0
12-13	36.006640182334436	37.0	37.0	37.0	33.0	37.0
14-15	35.94802322472211	37.0	37.0	37.0	33.0	37.0
16-17	35.9548454868406	37.0	37.0	37.0	33.0	37.0
18-19	35.9775443421572	37.0	37.0	37.0	33.0	37.0
20-21	35.99645390070922	37.0	37.0	37.0	33.0	37.0
22-23	36.00834256465106	37.0	37.0	37.0	33.0	37.0
24-25	35.98792034327769	37.0	37.0	37.0	33.0	37.0
26-27	35.941390682098884	37.0	37.0	37.0	33.0	37.0
28-29	35.91545513579587	37.0	37.0	37.0	33.0	37.0
30-31	35.87625705081681	37.0	37.0	37.0	33.0	37.0
32-33	35.795568967493026	37.0	37.0	37.0	33.0	37.0
34-35	35.67608643059959	37.0	37.0	37.0	33.0	37.0
36-37	35.565513070272615	37.0	37.0	37.0	33.0	37.0
38-39	35.538314425048064	37.0	37.0	37.0	33.0	37.0
40-41	35.428641277359716	37.0	37.0	37.0	33.0	37.0
42-43	35.37196353311178	37.0	37.0	37.0	33.0	37.0
44-45	35.257277461578624	37.0	37.0	37.0	33.0	37.0
46-47	35.08596585049085	37.0	37.0	37.0	33.0	37.0
48-49	34.89392897615137	37.0	37.0	37.0	27.0	37.0
50-51	34.76727657027898	37.0	37.0	37.0	27.0	37.0
52-53	34.65110330162853	37.0	37.0	37.0	27.0	37.0
54-55	34.600725772565795	37.0	37.0	37.0	27.0	37.0
56-57	34.58908285266817	37.0	37.0	37.0	27.0	37.0
58-59	34.536678253562016	37.0	37.0	37.0	27.0	37.0
60-61	34.49898137183747	37.0	37.0	37.0	27.0	37.0
62-63	34.48517386709449	37.0	37.0	37.0	27.0	37.0
64-65	34.44646345034825	37.0	37.0	37.0	27.0	37.0
66-67	34.42446744846379	37.0	37.0	37.0	27.0	37.0
68-69	34.3962030635242	37.0	37.0	37.0	27.0	37.0
70-71	34.28151953856144	37.0	37.0	37.0	27.0	37.0
72-73	34.28665087793015	37.0	37.0	37.0	27.0	37.0
74-75	34.20933445382431	37.0	37.0	37.0	27.0	37.0
76-77	34.08064097177127	37.0	37.0	37.0	27.0	37.0
78-79	34.08955014833772	37.0	37.0	37.0	27.0	37.0
80-81	34.07068770133822	37.0	37.0	37.0	27.0	37.0
82-83	33.98724168226441	37.0	37.0	37.0	27.0	37.0
84-85	33.913266358531644	37.0	37.0	37.0	24.5	37.0
86-87	33.78052128296217	37.0	37.0	37.0	22.0	37.0
88-89	33.6932146631524	37.0	35.0	37.0	22.0	37.0
90-91	33.62340680188956	37.0	33.0	37.0	22.0	37.0
92-93	33.44413588499688	37.0	33.0	37.0	18.0	37.0
94-95	33.4708392222774	37.0	33.0	37.0	18.0	37.0
96-97	33.45625374027529	37.0	33.0	37.0	14.0	37.0
98-99	33.43101977411921	37.0	33.0	37.0	14.0	37.0
100-101	33.29839184078841	37.0	33.0	37.0	14.0	37.0
102-103	33.219379400791986	37.0	33.0	37.0	14.0	37.0
104-105	33.04848797382125	37.0	33.0	37.0	14.0	37.0
106-107	33.01526541630059	37.0	33.0	37.0	14.0	37.0
108-109	32.88965710429479	37.0	33.0	37.0	14.0	37.0
110-111	32.8041598227587	37.0	33.0	37.0	14.0	37.0
112-113	32.664246151495476	37.0	33.0	37.0	14.0	37.0
114-115	32.627281408762755	37.0	33.0	37.0	14.0	37.0
116-117	32.46571424933471	37.0	33.0	37.0	14.0	37.0
118-119	32.33887467053745	37.0	33.0	37.0	14.0	37.0
120-121	32.24210372181265	37.0	33.0	37.0	14.0	37.0
122-123	32.15242879152501	37.0	33.0	37.0	14.0	37.0
124-125	30.748336452882082	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	161.0
16	64.0
17	26.0
18	140.0
19	418.0
20	1241.0
21	2753.0
22	4506.0
23	6156.0
24	5413.0
25	4193.0
26	3944.0
27	4437.0
28	5640.0
29	6990.0
30	9125.0
31	11938.0
32	16924.0
33	24947.0
34	39769.0
35	79863.0
36	164033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.37798059508738	13.919420450811426	12.14450011589965	21.55809883820154
2	18.82985089830271	20.78026917249195	37.60800641735742	22.78187351184792
3	20.53819493077113	26.76357265275044	28.687516393630485	24.010716022847948
4	14.88456308157746	18.652752099663342	33.82660778950907	32.63607702925013
5	16.169688264790295	35.19315053492346	31.98055379412386	16.65660740616238
6	29.841091983294287	34.015483345217476	18.780686564123457	17.362738107364777
7	26.199370997109643	32.51613888994996	22.333931777378815	18.95055833556158
8	26.401314030329655	31.390300113322382	21.04460318066644	21.163782675681524
9	27.473929485465447	15.793320345824261	20.54165552542114	36.19109464328915
10-11	25.86398767459923	24.987967454830205	26.16881215223398	22.979232718336583
12-13	27.220325732235423	24.055316672799666	26.526257084249572	22.198100510715346
14-15	25.125736913811323	24.02587315532806	25.771929154411293	25.076460776449316
16-17	23.35523383882756	26.252466990081107	26.637126449953524	23.755172721137807
18-19	23.268395787972548	24.466047850057933	29.571794186179762	22.693762175789757
20-21	24.992105611636838	23.08994000264844	29.597207933096332	22.32074645261839
22-23	24.889033194545245	22.9852171587914	29.40830436609496	22.717445280568395
24-25	23.81590842532819	23.809287342271794	29.448540178514587	22.92626405388543
26-27	23.317799253854872	23.8785540573233	29.81715624482728	22.98649044399455
28-29	23.238112215339367	23.85196691983982	29.877318189631573	23.032602675189242
30-31	23.848025577785727	23.554150978711917	29.80955448967301	22.788268953829345
32-33	23.432934073028093	23.72375278372332	29.606847227226947	23.23646591602164
34-35	23.59219221513427	23.83042387664413	29.6380050167437	22.9393788914779
36-37	23.66298006279875	23.910889954492557	29.37458458858672	23.051545394121973
38-39	23.487400842914806	23.90631167475203	29.245960502693	23.36032697964017
40-41	23.344445066950527	23.92953004751985	29.38667305018361	23.339351835346008
42-43	23.635125069235325	23.868392403532116	29.1515410032278	23.34494152400476
44-45	23.543043406292576	23.923119039433644	28.95539681933356	23.57844073494022
46-47	23.60390643900327	24.00091676534627	28.658848695519307	23.73632810013115
48-49	23.594102142938997	24.49138622560067	28.365101799151994	23.54940983230834
50-51	23.486000229191337	24.70020499891771	28.110954072602723	23.702840699288235
52-53	23.49160268408521	24.443128716401187	27.97764111183264	24.087627487680965
54-55	23.24089282758445	24.37322535874811	28.141767574519015	24.244114239148427
56-57	23.46626430854247	24.32738709143461	27.644422374167586	24.56192622585533
58-59	23.42577383908222	24.45051377057947	27.87756089486484	24.246151495473473
60-61	23.62389701669277	24.238766441295187	28.337598838763896	23.79973770324815
62-63	23.10185008340018	24.33871932974267	28.438061041292638	24.121369545564512
64-65	23.133809541999312	24.05019290270828	28.468110572087042	24.34788698320537
66-67	23.332824019252072	24.09883239746871	28.346257178145333	24.222086405133886
68-69	23.324674993951895	24.08597221691687	28.448247322917858	24.14110546621338
70-71	23.17990246635344	24.212664094630558	28.652609598023858	23.95482384099214
72-73	23.364528820810573	24.413843156728674	28.55571259406394	23.665915428396804
74-75	23.19490950975745	24.302656860796397	28.86246298589769	23.639970643548462
76-77	23.274200948157212	24.016796655961667	28.71081205077229	23.99819034510883
78-79	23.09828488483135	24.290207163502554	28.84436634961865	23.76714160204744
80-81	23.057030444249207	24.474960846480005	28.721748984555052	23.746259724715742
82-83	23.21835567948865	24.29835618880273	28.846403605943692	23.636884525764927
84-85	23.094673214190152	24.45038772925464	28.484020661904673	23.970918394650536
86-87	22.820316295962833	24.71028733426089	28.496988416594505	23.972407953181772
88-89	22.930880514377566	24.23213328910775	28.497818487995307	24.33916770851938
90-91	23.27040979522345	24.15825484351524	28.294055503377685	24.277279857883624
92-93	23.29643682205529	24.550076750863127	27.97785699350805	24.17562943357353
94-95	22.933932966541843	24.487215563639513	28.153644974527896	24.425206495290748
96-97	22.957714198403302	24.38277499777175	28.01813158129289	24.641379222532056
98-99	22.79715293428575	24.699059042234868	27.65435399875218	24.8494340247272
100-101	23.02659892789386	24.968740848262602	27.310821650941598	24.693838572901942
102-103	23.179520480792494	24.85936564931179	27.595146236805583	24.365967633090136
104-105	23.317811808836954	24.808209218435852	27.30374948590521	24.570229486821983
106-107	23.280237340361868	24.804869042616858	27.348765549995548	24.566128067025733
108-109	23.174231697751882	24.909978506891036	27.072531603019222	24.843258192337856
110-111	22.942816761526412	25.359766734150785	26.999630747291086	24.697785757031717
112-113	22.961279396972127	25.507340489196174	26.828373887467055	24.703006226364643
114-115	22.974776220125545	25.616970345187617	26.826463959662323	24.58178947502451
116-117	22.95044603407838	25.485951772804015	26.887845921519393	24.67575627159821
118-119	22.7693259720284	25.221424860702246	27.132758146499476	24.87649102076988
120-121	22.904745533952152	25.227854387104166	27.19431605485313	24.673084024090556
122-123	22.74469358391586	25.542865146364136	27.12619529648446	24.586245973235545
124-125	22.855476653411618	25.355091480223745	26.97765740850386	24.81177445786078
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	237.0
1	209.5
2	358.0
3	507.5
4	369.5
5	305.0
6	446.0
7	878.5
8	1498.0
9	1840.0
10	1579.5
11	1151.0
12	985.5
13	932.5
14	886.5
15	845.5
16	859.5
17	825.0
18	812.5
19	815.5
20	766.5
21	768.5
22	827.0
23	875.5
24	886.5
25	976.0
26	1116.5
27	1256.5
28	1432.0
29	1768.5
30	2309.0
31	2824.0
32	3365.0
33	3925.5
34	4441.0
35	5125.5
36	5812.0
37	6571.5
38	7315.0
39	7707.5
40	7883.0
41	8120.0
42	8749.0
43	9753.5
44	10691.5
45	11457.5
46	12669.5
47	14046.0
48	15215.0
49	16127.5
50	16538.5
51	16274.0
52	15954.0
53	16121.0
54	16167.0
55	15783.0
56	15155.5
57	14005.0
58	12704.5
59	11287.0
60	9800.5
61	8395.5
62	6951.5
63	5514.5
64	4107.0
65	3210.0
66	2543.5
67	2012.0
68	1682.5
69	1353.0
70	1084.0
71	956.5
72	863.0
73	780.0
74	605.0
75	492.0
76	401.0
77	274.5
78	199.0
79	125.5
80	96.0
81	78.0
82	53.5
83	36.5
84	22.5
85	17.0
86	11.5
87	6.0
88	5.5
89	4.0
90	2.0
91	2.5
92	1.5
93	1.0
94	1.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026484332225575204
2	0.0
3	5.093140812610617E-4
4	7.639711218915925E-4
5	0.00305588448756637
6	0.001273285203152654
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.273285203152654E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.546570406305308E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	6.36642601576327E-4
30-31	1.273285203152654E-4
32-33	1.273285203152654E-4
34-35	0.0
36-37	5.093140812610617E-4
38-39	0.0
40-41	0.0017825992844137158
42-43	6.36642601576327E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.06799342984835173
76-77	0.08785667901753315
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.06926671505150439
86-87	0.1567414085080917
88-89	0.19226606567605078
90-91	0.08416415192839044
92-93	0.12911111959967914
94-95	1.273285203152654E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0011459566828373887
106-107	0.0
108-109	2.546570406305308E-4
110-111	0.0
112-113	0.0
114-115	0.0
116-117	5.093140812610617E-4
118-119	2.546570406305308E-4
120-121	0.0
122-123	0.0
124-125	0.0011459566828373887
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	392685.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.84277650613828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.27686665524385	64.5720024872831
2	9.340085493774708	13.980758623176094
3	2.1294150126226237	4.781139956355919
4	0.8433517857270189	2.524751568608796
5	0.42447970614029223	1.5884619889024572
6	0.2456544506498873	1.1031276688636615
7	0.15626356931596025	0.8186639576056103
8	0.1127921009801246	0.6753339204250596
9	0.07570662774029624	0.509948479800036
>10	0.35783354955901453	4.639411536544525
>50	0.025672655434476586	1.2314248684886486
>100	0.009962523003409073	1.2199537471158861
>500	7.663479233391594E-4	0.36019320789317183
>1k	0.0011495218850087392	1.994827988937025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2851	0.7260272228376434	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2713	0.6908845512306302	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1392	0.3544826005576989	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	678	0.1726574735474999	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	578	0.14719176948444684	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	2.546570406305308E-4
14-15	0.0	1.273285203152654E-4	0.0	0.0	5.093140812610617E-4
16-17	0.0	2.546570406305308E-4	0.0	1.273285203152654E-4	5.093140812610617E-4
18-19	0.0	2.546570406305308E-4	0.0	2.546570406305308E-4	5.093140812610617E-4
20-21	0.0	2.546570406305308E-4	0.0	7.639711218915925E-4	5.093140812610617E-4
22-23	0.0	2.546570406305308E-4	0.0	0.0022919133656747774	5.093140812610617E-4
24-25	0.0	2.546570406305308E-4	0.0	0.0072577256579701285	5.093140812610617E-4
26-27	0.0	2.546570406305308E-4	0.0	0.011968880909634948	5.093140812610617E-4
28-29	0.0	2.546570406305308E-4	0.0	0.026102346664629412	5.093140812610617E-4
30-31	0.0	2.546570406305308E-4	0.0	0.05195003628862829	5.093140812610617E-4
32-33	0.0	2.546570406305308E-4	0.0	0.09753364656149331	5.093140812610617E-4
34-35	0.0	2.546570406305308E-4	0.0	0.14489985611877204	5.093140812610617E-4
36-37	0.0	2.546570406305308E-4	0.0	0.20881877331703527	5.093140812610617E-4
38-39	0.0	2.546570406305308E-4	0.0	0.2979487375377211	5.093140812610617E-4
40-41	0.0	2.546570406305308E-4	0.0	0.390516571806919	5.093140812610617E-4
42-43	0.0	2.546570406305308E-4	0.0	0.4796465360276048	5.093140812610617E-4
44-45	0.0	2.546570406305308E-4	0.0	0.5626647312731579	5.093140812610617E-4
46-47	0.0	2.546570406305308E-4	0.0	0.6624902912003259	5.093140812610617E-4
48-49	0.0	2.546570406305308E-4	0.0	0.7657537211760062	5.093140812610617E-4
50-51	0.0	2.546570406305308E-4	0.0	0.8546290283560615	5.093140812610617E-4
52-53	0.0	2.546570406305308E-4	0.0	0.9457962489017915	5.093140812610617E-4
54-55	0.0	2.546570406305308E-4	0.0	1.0442211951054916	5.093140812610617E-4
56-57	0.0	2.546570406305308E-4	0.0	1.1389536142200491	7.639711218915925E-4
58-59	0.0	2.546570406305308E-4	0.0	1.2415804015941532	7.639711218915925E-4
60-61	0.0	2.546570406305308E-4	0.0	1.3443345174885724	7.639711218915925E-4
62-63	0.0	2.546570406305308E-4	0.0	1.4407222073672283	8.912996422068579E-4
64-65	0.0	2.546570406305308E-4	0.0	1.5264143015394018	0.0010186281625221233
66-67	0.0	2.546570406305308E-4	0.0	1.6231839769790035	0.001273285203152654
68-69	0.0	2.546570406305308E-4	0.0	1.7382889593440036	0.001273285203152654
70-71	0.0	2.546570406305308E-4	0.0	1.8635802233342247	0.001273285203152654
72-73	0.0	2.546570406305308E-4	0.0	1.9757566497319736	0.001273285203152654
74-75	0.0	2.546570406305308E-4	0.0	2.095063473267377	0.001273285203152654
76-77	0.0	2.546570406305308E-4	0.0	2.2185721379731844	0.001273285203152654
78-79	0.0	2.546570406305308E-4	0.0	2.353667698027681	0.001273285203152654
80-81	0.0	2.546570406305308E-4	0.0	2.498058240065192	0.001273285203152654
82-83	0.0	2.546570406305308E-4	0.0	2.6486878795981514	0.001273285203152654
84-85	0.0	2.546570406305308E-4	0.0	2.780345569604136	0.001273285203152654
86-87	0.0	2.546570406305308E-4	0.0	2.9198976278696662	0.001273285203152654
88-89	0.0	2.546570406305308E-4	0.0	3.081222863109108	0.001273285203152654
90-91	0.0	2.546570406305308E-4	0.0	3.2532436940550316	0.001273285203152654
92-93	0.0	2.546570406305308E-4	0.0	3.427938423927576	0.001273285203152654
94-95	0.0	2.546570406305308E-4	0.0	3.5958847422234106	0.001273285203152654
96-97	0.0	2.546570406305308E-4	0.0	3.7587379197066353	0.0014006137234679195
98-99	0.0	2.546570406305308E-4	0.0	3.92910347988846	0.001527942243783185
100-101	0.0	2.546570406305308E-4	0.0	4.119332289239466	0.001527942243783185
102-103	0.0	2.546570406305308E-4	0.0	4.332352903726906	0.001527942243783185
104-105	0.0	2.546570406305308E-4	0.0	4.548302074181596	0.001527942243783185
106-107	0.0	2.546570406305308E-4	0.0	4.7713816417739405	0.001527942243783185
108-109	0.0	2.546570406305308E-4	0.0	4.993187924163133	0.001527942243783185
110-111	0.0	2.546570406305308E-4	0.0	5.214230235430434	0.001527942243783185
112-113	0.0	2.546570406305308E-4	0.0	5.441384315672868	0.001527942243783185
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1655	0.0	53.552494	1
GTATCAA	2695	0.0	45.025948	1
CCGTCGA	65	0.0019413114	36.600143	9
TATCAAC	3405	0.0	35.80741	2
ATCAACG	3475	0.0	35.08611	3
TCAACGC	3545	0.0	34.56107	4
CAACGCA	3565	0.0	34.200344	5
GTCAACG	105	1.48413965E-5	33.990177	1
GTCTAGA	125	1.278062E-6	33.31037	1
GCGCAGA	200	5.2750693E-11	32.71554	1
AACGCAG	3775	0.0	32.612907	6
GTACTAG	115	2.7648293E-5	31.034508	1
ACGCAGA	4115	0.0	29.918282	7
TAGCCCG	80	0.0053688735	29.737616	5
CGCAGAG	4215	0.0	29.208477	8
GTGGTAT	875	0.0	27.871946	1
GCAGAGT	4480	0.0	27.480745	9
GTATTCA	175	5.698712E-7	27.19214	1
TGGTATC	985	0.0	24.75619	2
CAGAGTA	4445	0.0	24.017557	10-11
>>END_MODULE