FastQCFastQC Report
Sun 1 Jan 2017
ERR1142567_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142567_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374113
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16190.4327569477671185No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6260.16732912248438306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22700.033.5930821
GGTATCA16400.033.056991
TATCAAC26200.028.192132
AACGCAG27100.027.6843366
ATCAACG26150.027.5626643
CAACGCA26750.027.1634735
TCAACGC27850.025.8802034
ACGCAGA30000.024.6145277
CGCAGAG30600.024.131898
GCAGAGT32150.023.1567889
CCTTAGT1400.003128721.2768691
CAGAGTA31300.020.64594310-11
GTTTAAT1450.003834382420.5431841
GTAAGTA1450.003837362920.5404283
GTAACAT1450.003837362920.5404283
ATAACCA1500.004669436319.8557454
ATGGGGG13650.019.83712818-19
GAGTACA32850.019.03720712-13
AATTCGA1452.1876494E-618.46657824-25
AGAGTAC30950.018.18529910-11
Produced by FastQC (version 0.11.5)