##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142567_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374113
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247577603558284	33.0	33.0	33.0	27.0	33.0
2	31.327123088478615	33.0	33.0	33.0	27.0	33.0
3	31.440104995014874	33.0	33.0	33.0	27.0	33.0
4	31.36170889544068	33.0	33.0	33.0	27.0	33.0
5	31.413123842261562	33.0	33.0	33.0	27.0	33.0
6	34.61959087227656	37.0	37.0	37.0	27.0	37.0
7	34.77236556869181	37.0	37.0	37.0	27.0	37.0
8	34.75137458468431	37.0	37.0	37.0	27.0	37.0
9	34.61824635872049	37.0	37.0	37.0	27.0	37.0
10-11	34.70543926567642	37.0	37.0	37.0	27.0	37.0
12-13	34.71114342458027	37.0	37.0	37.0	27.0	37.0
14-15	34.66135499167363	37.0	37.0	37.0	27.0	37.0
16-17	34.67353847634271	37.0	37.0	37.0	27.0	37.0
18-19	34.687507517782066	37.0	37.0	37.0	27.0	37.0
20-21	34.7550820206729	37.0	37.0	37.0	27.0	37.0
22-23	34.823693643364436	37.0	37.0	37.0	27.0	37.0
24-25	34.876017406505525	37.0	37.0	37.0	27.0	37.0
26-27	34.85613972249026	37.0	37.0	37.0	27.0	37.0
28-29	34.91519273588462	37.0	37.0	37.0	27.0	37.0
30-31	34.980770515860186	37.0	37.0	37.0	27.0	37.0
32-33	35.002638240317765	37.0	37.0	37.0	27.0	37.0
34-35	35.00710346873805	37.0	37.0	37.0	27.0	37.0
36-37	34.95661070318326	37.0	37.0	37.0	27.0	37.0
38-39	34.947212473236696	37.0	37.0	37.0	27.0	37.0
40-41	34.93500092218127	37.0	37.0	37.0	27.0	37.0
42-43	34.927215841203065	37.0	37.0	37.0	27.0	37.0
44-45	34.93831409226624	37.0	37.0	37.0	27.0	37.0
46-47	34.94419065897202	37.0	37.0	37.0	27.0	37.0
48-49	34.942724524408405	37.0	37.0	37.0	27.0	37.0
50-51	34.925597346256346	37.0	37.0	37.0	27.0	37.0
52-53	34.895651848505665	37.0	37.0	37.0	27.0	37.0
54-55	34.87501236257494	37.0	37.0	37.0	27.0	37.0
56-57	34.805850104112935	37.0	37.0	37.0	27.0	37.0
58-59	34.77968822254239	37.0	37.0	37.0	27.0	37.0
60-61	34.74510241558032	37.0	37.0	37.0	27.0	37.0
62-63	34.77974168232593	37.0	37.0	37.0	27.0	37.0
64-65	34.729007011250616	37.0	37.0	37.0	27.0	37.0
66-67	34.69261292711026	37.0	37.0	37.0	27.0	37.0
68-69	34.660966071748376	37.0	37.0	37.0	27.0	37.0
70-71	34.62937000318085	37.0	37.0	37.0	27.0	37.0
72-73	34.59035238016321	37.0	37.0	37.0	27.0	37.0
74-75	34.491621515424484	37.0	37.0	37.0	27.0	37.0
76-77	34.443765653692864	37.0	37.0	37.0	27.0	37.0
78-79	34.38586603512842	37.0	37.0	37.0	27.0	37.0
80-81	34.31480328136151	37.0	37.0	37.0	27.0	37.0
82-83	34.28690529332047	37.0	37.0	37.0	27.0	37.0
84-85	34.27175479066485	37.0	37.0	37.0	27.0	37.0
86-87	34.21768690208573	37.0	37.0	37.0	27.0	37.0
88-89	34.164816512657936	37.0	37.0	37.0	27.0	37.0
90-91	34.08441834419013	37.0	37.0	37.0	27.0	37.0
92-93	33.96819811126585	37.0	37.0	37.0	27.0	37.0
94-95	33.90730741781226	37.0	37.0	37.0	22.0	37.0
96-97	33.80499608407085	37.0	37.0	37.0	22.0	37.0
98-99	33.768765052270304	37.0	35.0	37.0	22.0	37.0
100-101	33.64435344401291	37.0	33.0	37.0	22.0	37.0
102-103	33.5948296370348	37.0	33.0	37.0	22.0	37.0
104-105	33.47799194361062	37.0	33.0	37.0	22.0	37.0
106-107	33.39138575777907	37.0	33.0	37.0	22.0	37.0
108-109	33.27111193676777	37.0	33.0	37.0	22.0	37.0
110-111	33.19136063168081	37.0	33.0	37.0	14.0	37.0
112-113	33.108337587841106	37.0	33.0	37.0	14.0	37.0
114-115	32.95638750858698	37.0	33.0	37.0	14.0	37.0
116-117	32.76527279191046	37.0	33.0	37.0	14.0	37.0
118-119	32.63307476618027	37.0	33.0	37.0	14.0	37.0
120-121	32.56372673497045	37.0	33.0	37.0	14.0	37.0
122-123	32.36623827560122	37.0	33.0	37.0	14.0	37.0
124-125	30.77267563543635	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	635.0
1	58.0
2	15.0
3	13.0
4	12.0
5	19.0
6	7.0
7	10.0
8	8.0
9	11.0
10	8.0
11	13.0
12	14.0
13	9.0
14	19.0
15	118.0
16	190.0
17	352.0
18	819.0
19	1461.0
20	2138.0
21	2750.0
22	3004.0
23	3420.0
24	3530.0
25	4053.0
26	4759.0
27	5538.0
28	6793.0
29	8120.0
30	9898.0
31	11762.0
32	14853.0
33	20534.0
34	30447.0
35	57901.0
36	180822.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.00353290537876	20.675946467228748	12.745821273079905	23.574699354312585
2	17.1425507386257	23.57274993967667	36.45700957130218	22.827689750395454
3	19.64211993673431	28.08996595448087	28.491542235208968	23.77637187357585
4	14.573676661796442	20.223047330536307	33.8889856976261	31.314290310041148
5	14.987652401011403	38.331988191351584	31.18110616253784	15.49925324509917
6	28.585297043908277	36.469102230946845	18.549120252298927	16.396480472845955
7	25.366605878566833	34.286901952370734	21.719856254022744	18.626635915039692
8	24.932284207872733	34.96816687316631	20.544569059380716	19.554979859580243
9	26.058806648267797	19.288505534412053	19.958481851902512	34.69420596541764
10-11	24.76140540497049	28.14524466925298	25.352108898636356	21.741241027140177
12-13	25.53410608636883	27.159220738817698	25.21683392961547	22.089839245198007
14-15	24.11529818125258	27.168980997280727	23.784775298073985	24.930945523392705
16-17	23.529419648301754	28.988636348406686	24.310628813738774	23.171315189552786
18-19	23.61937538194333	28.276269660030234	25.680931244840416	22.423423713186025
20-21	25.521001523509888	26.755620706335094	25.3368939961383	22.386483774016718
22-23	25.772491387730984	26.472460167837074	24.96546382981004	22.789584614621898
24-25	24.536699011436998	27.063369045210784	25.116520215369814	23.2834117279824
26-27	23.734242930503434	27.516743456353137	25.193358618777058	23.555654994366375
28-29	23.642637738897232	27.378590574987644	25.568417005691874	23.410354680423254
30-31	24.34263668696124	26.936457223997163	25.606258497933705	23.114647591107897
32-33	24.033719033062656	27.094445977479566	25.335087244582212	23.53674774487557
34-35	23.877961669439777	27.460633876970313	25.416569540788846	23.244834912801057
36-37	23.970384143433556	27.39748048204134	25.154723694292773	23.47741168023234
38-39	23.850846821989915	27.310996966307936	25.232549573742823	23.605606637959323
40-41	23.562225952476325	27.17519884144798	25.48586708536015	23.776708120715547
42-43	23.813299828816206	27.26939078393605	25.32555728495538	23.59175210229236
44-45	23.82976671675734	27.381784674397235	25.326459928801604	23.46198868004382
46-47	23.784381090149278	27.25426770873097	25.251173661757715	23.710177539362036
48-49	23.75814332247557	27.32888307903309	25.407567932453283	23.505405666038058
50-51	23.467352769031102	27.555438883628234	25.316186131606827	23.661022215733833
52-53	23.348684025409703	27.473004532853473	25.23056185676826	23.94774958496856
54-55	23.07769598156571	27.353620877906316	25.322365644697797	24.24631749583018
56-57	23.526086135222144	27.068811450757003	25.107880031777885	24.297222382242968
58-59	23.415257575067454	27.246932864633433	25.444831752989433	23.89297780730968
60-61	23.66552644025602	27.139398373134068	25.780371759365334	23.41470342724458
62-63	23.459553740796707	27.136181933146858	25.564120288503787	23.840144037552648
64-65	23.192108485548392	27.063778934704498	25.67850358108416	24.065608998662956
66-67	23.826212303285814	26.935592557314546	25.331602476250954	23.906592663148686
68-69	23.60877800729268	26.942325673608376	25.231546600009107	24.217349719089835
70-71	23.550136372648307	27.041570884305628	25.422782002025496	23.985510741020573
72-73	23.85954148571061	27.30670410369485	25.277629678847095	23.55612473174744
74-75	23.76036572543	27.044447212656266	25.65877860243701	23.536408459476725
76-77	23.631351294873152	26.779956625466667	25.553467350550086	24.035224729110098
78-79	23.598925573886888	27.024094206538997	25.55231798725961	23.824662232314502
80-81	23.51607164863663	27.166417514587454	25.524290988196086	23.793219848579835
82-83	23.568444829092233	26.86843705873054	25.927419462880447	23.63569864929678
84-85	23.57351706180879	27.224022212429798	25.36267309930633	23.839787626455077
86-87	23.10541936313376	27.50061608022972	25.50331076158231	23.890653795054213
88-89	23.473093032195674	26.796331592531924	25.58187816354604	24.148697211726365
90-91	23.791309751485247	26.68533607615619	25.543475349415274	23.979878822943284
92-93	23.68748894854442	26.972651331265034	25.542124129949045	23.797735590241498
94-95	23.039318107544855	27.06140139400076	25.858258731255724	24.041021767198657
96-97	23.049314863026176	27.145271537085247	25.591660506326402	24.213753093562175
98-99	22.90012855535915	27.339439712893032	25.490786865927472	24.269644865820343
100-101	23.142782157854143	27.485485045847973	25.381218399177307	23.990514397120577
102-103	23.232538301978003	27.534840371041412	25.61696281149441	23.61565851548617
104-105	23.35634763558843	27.58248056327014	25.593278895313492	23.467892905827938
106-107	23.162205340200416	27.47287917927127	25.663708518636064	23.70120696189225
108-109	23.173635214466298	27.56633778977589	25.42381732870549	23.836209667052323
110-111	22.79164931515654	28.332527687654068	25.27285947084478	23.602963526344613
112-113	22.881253799615955	28.342122107270356	25.034347189154595	23.7422769039591
114-115	22.967993487295942	28.23425917991345	25.041373880628992	23.75637345216162
116-117	23.076665559685903	28.13869219150053	25.081684467631526	23.70295778118204
118-119	22.843352523764896	27.939215423751506	25.36377025036819	23.85366180211541
120-121	23.03552898487658	27.86176700966051	25.53025687717161	23.5724471282913
122-123	22.934449474398782	28.279902900011916	25.497449987213116	23.288197638376186
124-125	22.90209509324022	28.139426527136596	25.069488330244965	23.88899004937822
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1645.0
1	1065.5
2	400.0
3	268.0
4	191.5
5	160.5
6	141.5
7	118.0
8	118.0
9	121.5
10	113.5
11	104.5
12	113.0
13	117.5
14	107.5
15	114.0
16	138.5
17	157.5
18	167.0
19	200.0
20	267.5
21	321.0
22	381.5
23	458.5
24	560.0
25	707.0
26	889.0
27	1095.5
28	1436.0
29	2030.5
30	2746.0
31	3456.5
32	4259.0
33	5062.5
34	5857.0
35	6616.0
36	7349.5
37	8229.0
38	8847.5
39	9218.5
40	9344.5
41	9537.0
42	10022.5
43	10624.5
44	11846.0
45	12954.0
46	13944.5
47	15090.5
48	16034.5
49	16457.5
50	16566.5
51	16274.0
52	15596.5
53	15322.5
54	14887.0
55	14188.5
56	13279.0
57	11571.5
58	9971.0
59	8753.0
60	7365.0
61	6051.5
62	4914.5
63	3955.5
64	3003.5
65	2342.5
66	1832.0
67	1384.5
68	1160.0
69	985.0
70	845.0
71	721.0
72	599.5
73	517.5
74	418.0
75	345.5
76	271.5
77	190.0
78	131.0
79	84.0
80	58.5
81	48.0
82	38.0
83	24.5
84	13.5
85	6.0
86	3.5
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.5
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3560421583853007
2	0.3001766845846041
3	0.28948472787633683
4	0.29349421164193706
5	0.31167053804599143
6	0.32663927743756566
7	0.3295795655323392
8	0.3290449676969258
9	0.3378658319812463
10-11	0.3347918944276195
12-13	0.3333217503802327
14-15	0.3258373806844456
16-17	0.2773226271206828
18-19	0.27291219497852254
20-21	0.257007909374975
22-23	0.2548695180333215
24-25	0.23909888188862724
26-27	0.2425737678188141
28-29	0.23027801760430672
30-31	0.23001071868660006
32-33	0.22773867788609325
34-35	0.23682684108812044
36-37	0.23174816165169349
38-39	0.21530927821273252
40-41	0.23749508838238714
42-43	0.22185810169654624
44-45	0.21116614498827893
46-47	0.21798226738979934
48-49	0.21410643308305247
50-51	0.21290358795337236
52-53	0.25353302344478806
54-55	0.2393661808063339
56-57	0.23990077864174728
58-59	0.23575764541729372
60-61	0.25166193102084133
62-63	0.2344211508287603
64-65	0.24110362377142736
66-67	0.23722778946468046
68-69	0.23041166706316007
70-71	0.23281735732252018
72-73	0.2309462648985734
74-75	0.22118985440227953
76-77	0.22787232734494656
78-79	0.2380296862178005
80-81	0.22640218329755984
82-83	0.240569025936014
84-85	0.21611117496585255
86-87	0.20982965039974552
88-89	0.21851686522521271
90-91	0.2071566612226787
92-93	0.22960977031003998
94-95	0.2135718352476391
96-97	0.20234528070395844
98-99	0.19593010667899807
100-101	0.18844573698321096
102-103	0.195529158302438
104-105	0.19298981858422454
106-107	0.19713295180867813
108-109	0.19272251966651788
110-111	0.18884668535977098
112-113	0.1924552207488112
114-115	0.18416895429990404
116-117	0.1937917153373446
118-119	0.1772191824395303
120-121	0.18323340808793065
122-123	0.1826988102525173
124-125	0.1788229759457704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	374113.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.11652915982141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.09944169861546	67.05823723016651
2	8.27757080323285	12.60119918833519
3	1.7096918385404138	3.904074260477102
4	0.6081838349720055	1.8517137043671466
5	0.29637408474171617	1.1279483331729112
6	0.17289692392550796	0.789618825097161
7	0.12255309080455867	0.6529821136896002
8	0.09820191660805312	0.5979831239237782
9	0.06785640363171913	0.46484945331429245
>10	0.5007294078329596	7.329188986568114
>50	0.033495760619296114	1.7376144663676565
>100	0.012216100931495971	1.211200969287567
>500	3.9406777198374105E-4	0.1877691448177357
>1k	3.9406777198374105E-4	0.4856202004151983
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1619	0.4327569477671185	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	626	0.16732912248438306	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.672989177066822E-4	0.0
10-11	0.0	0.0	0.0	2.672989177066822E-4	0.0
12-13	0.0	0.0	0.0	2.672989177066822E-4	0.0
14-15	0.0	0.0	0.0	2.672989177066822E-4	0.0
16-17	0.0	0.0	0.0	2.672989177066822E-4	0.0
18-19	0.0	0.0	0.0	5.345978354133644E-4	0.0
20-21	0.0	0.0	0.0	6.682472942667055E-4	0.0
22-23	0.0	0.0	0.0	0.0014701440473867521	1.336494588533411E-4
24-25	0.0	0.0	0.0	0.006949771860373738	2.672989177066822E-4
26-27	0.0	0.0	0.0	0.011493853461387335	2.672989177066822E-4
28-29	0.0	0.0	0.0	0.02071566612226787	2.672989177066822E-4
30-31	0.0	0.0	0.0	0.04062943549141569	2.672989177066822E-4
32-33	0.0	0.0	0.0	0.0721707077808042	2.672989177066822E-4
34-35	0.0	0.0	0.0	0.1073205154592329	2.672989177066822E-4
36-37	0.0	0.0	0.0	0.16492343222502293	2.672989177066822E-4
38-39	0.0	0.0	0.0	0.24751879779638775	2.672989177066822E-4
40-41	0.0	0.0	0.0	0.3290449676969258	2.672989177066822E-4
42-43	0.0	0.0	0.0	0.3981417379241031	2.672989177066822E-4
44-45	0.0	0.0	0.0	0.4663029619393071	2.672989177066822E-4
46-47	0.0	0.0	0.0	0.5529078112762722	2.672989177066822E-4
48-49	0.0	0.0	0.0	0.6515411119100378	2.672989177066822E-4
50-51	0.0	0.0	0.0	0.7370767655761762	2.672989177066822E-4
52-53	0.0	0.0	0.0	0.816197245217354	2.672989177066822E-4
54-55	0.0	0.0	0.0	0.9011983010480791	2.672989177066822E-4
56-57	0.0	0.0	0.0	0.9912780363152309	2.672989177066822E-4
58-59	0.0	0.0	0.0	1.0932525734203302	2.672989177066822E-4
60-61	0.0	0.0	0.0	1.1905493794655624	2.672989177066822E-4
62-63	0.0	0.0	0.0	1.2736793428723407	2.672989177066822E-4
64-65	0.0	0.0	0.0	1.3570766051968257	2.672989177066822E-4
66-67	0.0	0.0	0.0	1.4460871447931507	2.672989177066822E-4
68-69	0.0	0.0	0.0	1.5616939267012908	2.672989177066822E-4
70-71	0.0	0.0	0.0	1.6869234696468713	2.672989177066822E-4
72-73	0.0	0.0	0.0	1.7918382948467442	4.009483765600233E-4
74-75	0.0	0.0	0.0	1.89207538898675	5.345978354133644E-4
76-77	0.0	0.0	0.0	2.00567742901209	5.345978354133644E-4
78-79	0.0	0.0	0.0	2.134649156805564	5.345978354133644E-4
80-81	0.0	0.0	0.0	2.2672294199880785	5.345978354133644E-4
82-83	0.0	0.0	0.0	2.405823908818993	5.345978354133644E-4
84-85	0.0	0.0	0.0	2.5373349763306807	5.345978354133644E-4
86-87	0.0	0.0	0.0	2.6661730546653017	5.345978354133644E-4
88-89	0.0	0.0	0.0	2.80810877996755	5.345978354133644E-4
90-91	0.0	0.0	0.0	2.9552568341650787	5.345978354133644E-4
92-93	0.0	0.0	0.0	3.1122949483177544	5.345978354133644E-4
94-95	0.0	0.0	0.0	3.2834999051088842	5.345978354133644E-4
96-97	0.0	0.0	0.0	3.4508290275932674	5.345978354133644E-4
98-99	0.0	0.0	0.0	3.6319240443395446	5.345978354133644E-4
100-101	0.0	0.0	0.0	3.8282550993951023	5.345978354133644E-4
102-103	0.0	0.0	0.0	4.017903681507994	5.345978354133644E-4
104-105	0.0	0.0	0.0	4.2197143643765385	5.345978354133644E-4
106-107	0.0	0.0	0.0	4.435157292048125	5.345978354133644E-4
108-109	0.0	0.0	0.0	4.647258983248377	5.345978354133644E-4
110-111	0.0	0.0	0.0	4.86858248710951	5.345978354133644E-4
112-113	0.0	0.0	0.0	5.093113577983123	5.345978354133644E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2270	0.0	33.593082	1
GGTATCA	1640	0.0	33.05699	1
TATCAAC	2620	0.0	28.19213	2
AACGCAG	2710	0.0	27.684336	6
ATCAACG	2615	0.0	27.562664	3
CAACGCA	2675	0.0	27.163473	5
TCAACGC	2785	0.0	25.880203	4
ACGCAGA	3000	0.0	24.614527	7
CGCAGAG	3060	0.0	24.13189	8
GCAGAGT	3215	0.0	23.156788	9
CCTTAGT	140	0.0031287	21.276869	1
CAGAGTA	3130	0.0	20.645943	10-11
GTTTAAT	145	0.0038343824	20.543184	1
GTAAGTA	145	0.0038373629	20.540428	3
GTAACAT	145	0.0038373629	20.540428	3
ATAACCA	150	0.0046694363	19.855745	4
ATGGGGG	1365	0.0	19.837128	18-19
GAGTACA	3285	0.0	19.037207	12-13
AATTCGA	145	2.1876494E-6	18.466578	24-25
AGAGTAC	3095	0.0	18.185299	10-11
>>END_MODULE