FastQCFastQC Report
Sun 1 Jan 2017
ERR1142567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374113
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23190.619866190161796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22050.5893941135432343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12620.33733123414583294No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.1726751008385167No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.12349209998048717No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.10023709414000583No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACTA602.1967177E-549.5596669
GGTATCA17900.048.1817551
GTATCAA26000.037.0603371
TATCAAC32750.029.962792
CAACGCA34150.028.9085965
ATCAACG33900.028.770923
TCAACGC35350.027.9272564
AACGCAG35950.027.7920136
TCCGACC900.00954308926.4318228
ACGCAGA38250.025.8098967
CGCAGAG39250.025.1523198
CTGTACG1451.3420587E-424.6089389
GAGTACT17300.024.579312-13
GCAGAGT40650.024.4323679
TATTCTC2552.4796464E-823.3221975
CAGAGTA41150.022.7625210-11
ATGGGGG12350.021.18826518-19
AGTACTT18550.020.03760112-13
GTACTTT21300.019.9634714-15
TCGGAGA1500.00471307519.8238662
Produced by FastQC (version 0.11.5)