##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142567_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374113
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.609815750856026	33.0	27.0	33.0	14.0	33.0
2	30.57937307711841	33.0	27.0	33.0	27.0	33.0
3	30.12186692256083	33.0	27.0	33.0	27.0	33.0
4	31.133034671342617	33.0	33.0	33.0	27.0	33.0
5	31.87601874300011	33.0	33.0	33.0	27.0	33.0
6	34.18589570530829	37.0	33.0	37.0	27.0	37.0
7	35.52304517619008	37.0	37.0	37.0	33.0	37.0
8	35.267010234875556	37.0	37.0	37.0	33.0	37.0
9	35.81170127742153	37.0	37.0	37.0	33.0	37.0
10-11	35.87644775776303	37.0	37.0	37.0	33.0	37.0
12-13	35.97787700507601	37.0	37.0	37.0	33.0	37.0
14-15	36.024369642327315	37.0	37.0	37.0	37.0	37.0
16-17	36.0768217089489	37.0	37.0	37.0	37.0	37.0
18-19	36.11300195395509	37.0	37.0	37.0	37.0	37.0
20-21	36.13820423241106	37.0	37.0	37.0	37.0	37.0
22-23	36.1370468280974	37.0	37.0	37.0	37.0	37.0
24-25	36.12322881054655	37.0	37.0	37.0	37.0	37.0
26-27	36.090003555075604	37.0	37.0	37.0	37.0	37.0
28-29	36.06381093412953	37.0	37.0	37.0	37.0	37.0
30-31	36.030441337243026	37.0	37.0	37.0	37.0	37.0
32-33	35.97028838880232	37.0	37.0	37.0	37.0	37.0
34-35	35.8495962449848	37.0	37.0	37.0	33.0	37.0
36-37	35.75569680818363	37.0	37.0	37.0	33.0	37.0
38-39	35.73525111396824	37.0	37.0	37.0	33.0	37.0
40-41	35.63278741984374	37.0	37.0	37.0	33.0	37.0
42-43	35.58042088887582	37.0	37.0	37.0	33.0	37.0
44-45	35.47652447255241	37.0	37.0	37.0	33.0	37.0
46-47	35.33554701387014	37.0	37.0	37.0	33.0	37.0
48-49	35.17846078591228	37.0	37.0	37.0	33.0	37.0
50-51	35.07871418528626	37.0	37.0	37.0	33.0	37.0
52-53	34.99765311550253	37.0	37.0	37.0	30.0	37.0
54-55	34.94004485275839	37.0	37.0	37.0	27.0	37.0
56-57	34.93895694616333	37.0	37.0	37.0	27.0	37.0
58-59	34.90175027331314	37.0	37.0	37.0	27.0	37.0
60-61	34.85441564447106	37.0	37.0	37.0	27.0	37.0
62-63	34.835259667533606	37.0	37.0	37.0	27.0	37.0
64-65	34.80095051495137	37.0	37.0	37.0	27.0	37.0
66-67	34.77945968196775	37.0	37.0	37.0	27.0	37.0
68-69	34.75536669402025	37.0	37.0	37.0	27.0	37.0
70-71	34.64320272217218	37.0	37.0	37.0	27.0	37.0
72-73	34.642414190364946	37.0	37.0	37.0	27.0	37.0
74-75	34.5631039284922	37.0	37.0	37.0	27.0	37.0
76-77	34.45220695351405	37.0	37.0	37.0	27.0	37.0
78-79	34.44867593481114	37.0	37.0	37.0	27.0	37.0
80-81	34.451553407660256	37.0	37.0	37.0	27.0	37.0
82-83	34.375192789344396	37.0	37.0	37.0	27.0	37.0
84-85	34.31010149339905	37.0	37.0	37.0	27.0	37.0
86-87	34.16685199391628	37.0	37.0	37.0	27.0	37.0
88-89	34.07923942765956	37.0	37.0	37.0	27.0	37.0
90-91	34.028380462587506	37.0	37.0	37.0	27.0	37.0
92-93	33.86791824929901	37.0	37.0	37.0	24.5	37.0
94-95	33.93146990347836	37.0	37.0	37.0	22.0	37.0
96-97	33.937162033930925	37.0	37.0	37.0	22.0	37.0
98-99	33.89421645331758	37.0	37.0	37.0	22.0	37.0
100-101	33.75258544878152	37.0	35.0	37.0	22.0	37.0
102-103	33.68572864348472	37.0	33.0	37.0	22.0	37.0
104-105	33.54527642717575	37.0	33.0	37.0	22.0	37.0
106-107	33.502975036954076	37.0	33.0	37.0	22.0	37.0
108-109	33.393703239395585	37.0	33.0	37.0	18.0	37.0
110-111	33.335788919390666	37.0	33.0	37.0	14.0	37.0
112-113	33.18065263703747	37.0	33.0	37.0	14.0	37.0
114-115	33.15193537781366	37.0	33.0	37.0	14.0	37.0
116-117	33.00972834410993	37.0	33.0	37.0	14.0	37.0
118-119	32.881180285101024	37.0	33.0	37.0	14.0	37.0
120-121	32.80471942969103	37.0	33.0	37.0	14.0	37.0
122-123	32.73197269274257	37.0	33.0	37.0	14.0	37.0
124-125	31.381161574176787	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	4.0
17	9.0
18	58.0
19	270.0
20	931.0
21	2176.0
22	3505.0
23	4849.0
24	4227.0
25	3212.0
26	3215.0
27	3645.0
28	4576.0
29	5746.0
30	7704.0
31	10330.0
32	14495.0
33	21761.0
34	35221.0
35	71819.0
36	176357.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.050957798067024	17.585520071651047	13.322817383399949	25.040704746881975
2	17.560202398740486	21.04845327481269	38.19086746517763	23.20047686126919
3	20.504609594478644	25.45020060891019	29.447944593984136	24.59724520262703
4	14.706181102783153	18.132731724528746	35.502314839565045	31.658772333123053
5	15.62404768905878	34.48822475874789	33.00008019460557	16.887647357587745
6	28.99590492585029	34.77418285628749	18.91352229837373	17.31638991948849
7	25.160847123729997	32.593360829482	23.234423823817938	19.011368222970066
8	25.52945233124751	31.93126141032255	21.712958384231502	20.826327874198437
9	26.70235998214443	16.139775950047177	21.44726326003106	35.71060080777733
10-11	25.303852044703074	25.383106173803107	26.7551515183915	22.557890263102326
12-13	26.591297281837306	24.514251041797532	26.952952717494448	21.941498958870714
14-15	24.635738399895217	24.60780566299487	26.092918449773194	24.663537487336715
16-17	23.05225961140083	26.680976068727897	26.89508250181095	23.37168181806032
18-19	22.92769831574952	25.14774947676237	29.764670032851036	22.159882174637076
20-21	24.342807806784357	24.0358129483134	29.702454221413195	21.91892502348905
22-23	24.623576299139565	23.777575224597918	29.408761523924586	22.190086952337932
24-25	23.55865741099614	24.523606503917268	29.525838449880116	22.391897635206476
26-27	22.916338111746988	24.851448626484512	29.589455592294307	22.642757669474197
28-29	22.717862426875772	24.79173711229289	29.89028525299614	22.600115207835202
30-31	23.328677870961275	24.330381903839086	29.928965217681846	22.411975007517793
32-33	22.96096613037307	24.619163831055488	29.654701412147734	22.76516862642371
34-35	23.062817918650246	24.69588065637922	29.719229216840905	22.52207220812963
36-37	23.124441171478328	24.832234498815183	29.398289533651333	22.645034796055157
38-39	23.05974398109662	24.605533622194365	29.494564476508433	22.840157920200582
40-41	22.765205583444263	24.57819441548652	29.656701576558515	22.9998984245107
42-43	23.102080420842167	24.622902003972126	29.29696584132347	22.978051733862237
44-45	23.105318446565608	24.849443884601712	29.11660380687119	22.928633861961494
46-47	23.06776294862782	24.62264075292759	28.912521083202137	23.397075215242452
48-49	23.192190594820282	25.08426598380703	28.645222165495454	23.078321255877235
50-51	23.105853044401023	25.251060508456003	28.493396380238057	23.149690066904917
52-53	22.961511628839414	25.148952321892047	28.32526536100055	23.56427068826798
54-55	22.805676359816417	25.1390622619369	28.33689286392079	23.718368514325885
56-57	23.119618938662917	24.876975673125497	27.969624150991812	24.03378123721977
58-59	23.00935813510891	25.063951266061324	28.28958095548671	23.637109643343056
60-61	23.140200955326332	25.001136020400256	28.633594662575213	23.225068361698202
62-63	22.697019349768652	24.94780988631777	28.63038707556273	23.724783688350843
64-65	22.614156685279582	24.799592636449415	28.698815598495642	23.88743507977536
66-67	22.97140168879456	24.865214520746406	28.63025342610388	23.533130364355152
68-69	22.88519778783416	24.635604750436364	28.622234458572677	23.8569630031568
70-71	22.927965614667226	24.890741567387394	28.530684579258136	23.650608238687244
72-73	23.09569568552817	25.048715227752044	28.727683881607962	23.127905205111823
74-75	22.973607501040103	24.94973398811809	28.889947198949063	23.186711311892743
76-77	22.912471180775317	24.75502822711757	28.766369921761576	23.566130670345537
78-79	22.725517057034985	25.195362357579604	28.697383808346423	23.38173677703899
80-81	22.701162482993105	25.219118287790053	28.69600895985972	23.38371026935712
82-83	22.844167403966182	24.911724532427368	29.10524360286865	23.1388644607378
84-85	22.800239720896386	25.19249909034481	28.594208171914126	23.413053016844675
86-87	22.39395477600286	25.577019148477376	28.607131424959046	23.421894650560713
88-89	22.737456217690866	24.901581924840478	28.618153947576463	23.74280790989219
90-91	22.85641273328365	24.884972044092603	28.48559123499686	23.773023987626885
92-93	22.944351242031864	25.056334275473603	28.406801083438992	23.59251339905554
94-95	22.418613726942006	25.127702740630454	28.574941661699338	23.878741870728206
96-97	22.485452256403814	25.04898252666975	28.308826477561595	24.156738739364844
98-99	22.37732984419146	25.311202764940006	28.12639496622678	24.185072424641753
100-101	22.793113310684205	25.506330974865882	27.793206865305404	23.907348849144512
102-103	22.79698914499095	25.470780218810894	28.011456431612906	23.720774204585247
104-105	22.888505160288368	25.606845100465907	27.826931867086152	23.67771787215957
106-107	22.802468772803937	25.314677650870195	27.936211786278477	23.946641790047394
108-109	22.79740878697604	25.545794624850142	27.678185990502808	23.97861059767101
110-111	22.60573676937182	26.103610406481465	27.50358848797021	23.787064336176503
112-113	22.586090298920382	26.067391403132206	27.249921815066568	24.09659648288084
114-115	22.596648606169794	26.002838714506044	27.370072678575724	24.030440000748438
116-117	22.724848808847607	25.942731130341805	27.25744261418691	24.074977446623677
118-119	22.471850045106752	25.595108423268403	27.66026262153764	24.272778910087204
120-121	22.575398342212114	25.66938331466696	27.827020178395294	23.928198164725632
122-123	22.423438907495864	26.042799902703194	27.69591005926017	23.83785113054077
124-125	22.462481004943726	25.910392785019397	27.5268937920748	24.100232417962072
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	275.0
2	334.5
3	437.5
4	315.0
5	232.5
6	371.5
7	757.0
8	1232.0
9	1425.0
10	1245.0
11	951.0
12	795.0
13	742.0
14	722.5
15	691.5
16	669.0
17	675.0
18	657.5
19	638.5
20	638.5
21	645.5
22	685.5
23	730.5
24	758.0
25	846.5
26	989.5
27	1182.0
28	1471.0
29	1864.5
30	2389.5
31	3098.0
32	3901.0
33	4517.0
34	5239.5
35	6107.5
36	6758.0
37	7637.0
38	8407.5
39	8703.0
40	8782.5
41	8894.0
42	9361.5
43	10227.5
44	11424.0
45	12368.5
46	13392.0
47	14648.5
48	15652.5
49	16159.0
50	16428.5
51	16126.0
52	15422.5
53	15092.0
54	14711.5
55	14052.0
56	13272.5
57	11869.0
58	10153.5
59	8835.5
60	7602.0
61	6306.0
62	5030.0
63	3960.5
64	3009.5
65	2268.0
66	1747.5
67	1358.5
68	1136.0
69	965.5
70	775.0
71	658.5
72	566.0
73	494.0
74	393.5
75	303.0
76	245.0
77	191.0
78	132.0
79	77.0
80	46.5
81	25.5
82	16.5
83	12.5
84	7.5
85	5.5
86	5.0
87	2.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020849315581121214
2	0.0
3	5.345978354133644E-4
4	0.001336494588533411
5	0.006147875107253691
6	0.001336494588533411
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.682472942667055E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0017374429650934345
30-31	1.336494588533411E-4
32-33	5.345978354133644E-4
34-35	0.0
36-37	0.00120284512968007
38-39	0.0
40-41	0.0018710924239467755
42-43	0.001603793506240093
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.09395556957389879
76-77	0.11961626567374029
78-79	1.336494588533411E-4
80-81	0.0
82-83	0.0
84-85	0.09221812660880536
86-87	0.21210169120025232
88-89	0.25660696099841496
90-91	0.10678591762381955
92-93	0.1797585221577438
94-95	5.345978354133644E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.001603793506240093
106-107	0.0
108-109	6.682472942667055E-4
110-111	0.0
112-113	0.0
114-115	0.0
116-117	1.336494588533411E-4
118-119	1.336494588533411E-4
120-121	0.0
122-123	0.0
124-125	6.682472942667055E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	374113.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.30825467904188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.8277977734059	62.7836651461978
2	9.0231307951867	13.048936790813311
3	1.9583028777267106	4.248043896640908
4	0.6746051569964983	1.9511808599959133
5	0.3504097761564521	1.2668759668173397
6	0.1988979947005998	0.8629180113577016
7	0.1428867202966761	0.7232322553025553
8	0.1062833377139807	0.6148130121248907
9	0.07823244693342864	0.5091166527324289
>10	0.5804818527753887	8.111188115896857
>50	0.04095226951931299	1.9937672973772307
>100	0.016380907807585873	1.9816264301143323
>500	4.0952269518964684E-4	0.19129268886040923
>1k	0.0012285680855689406	1.7133428757683093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2319	0.619866190161796	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2205	0.5893941135432343	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1262	0.33733123414583294	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	646	0.1726751008385167	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	462	0.12349209998048717	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.10023709414000583	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.672989177066822E-4	0.0
10-11	0.0	0.0	0.0	2.672989177066822E-4	0.0
12-13	0.0	0.0	0.0	2.672989177066822E-4	0.0
14-15	0.0	0.0	0.0	2.672989177066822E-4	0.0
16-17	0.0	0.0	0.0	2.672989177066822E-4	0.0
18-19	0.0	0.0	0.0	5.345978354133644E-4	0.0
20-21	0.0	0.0	0.0	6.682472942667055E-4	1.336494588533411E-4
22-23	0.0	0.0	0.0	0.0014701440473867521	2.672989177066822E-4
24-25	0.0	0.0	0.0	0.007350720236933761	2.672989177066822E-4
26-27	0.0	0.0	0.0	0.012295750214507382	2.672989177066822E-4
28-29	0.0	0.0	0.0	0.02205216071080128	2.672989177066822E-4
30-31	0.0	0.0	0.0	0.04236687845650913	2.672989177066822E-4
32-33	0.0	0.0	0.0	0.07417544966360431	2.672989177066822E-4
34-35	0.0	0.0	0.0	0.10932525734203302	2.672989177066822E-4
36-37	0.0	0.0	0.0	0.16706182356667637	2.672989177066822E-4
38-39	0.0	0.0	0.0	0.24938989022033448	2.672989177066822E-4
40-41	0.0	0.0	0.0	0.33118335903857926	2.672989177066822E-4
42-43	0.0	0.0	0.0	0.40068107764231664	2.672989177066822E-4
44-45	0.0	0.0	0.0	0.4681740543632539	2.672989177066822E-4
46-47	0.0	0.0	0.0	0.5547789037002189	2.672989177066822E-4
48-49	0.0	0.0	0.0	0.653946802169398	2.672989177066822E-4
50-51	0.0	0.0	0.0	0.739749754753243	2.672989177066822E-4
52-53	0.0	0.0	0.0	0.8186029354767143	2.672989177066822E-4
54-55	0.0	0.0	0.0	0.9060096815667993	2.672989177066822E-4
56-57	0.0	0.0	0.0	0.9976932103401914	4.009483765600233E-4
58-59	0.0	0.0	0.0	1.1004696441984105	5.345978354133644E-4
60-61	0.0	0.0	0.0	1.1981673986202028	5.345978354133644E-4
62-63	0.0	0.0	0.0	1.2814310114858345	5.345978354133644E-4
64-65	0.0	0.0	0.0	1.3641600265160525	5.345978354133644E-4
66-67	0.0	0.0	0.0	1.452903267194671	5.345978354133644E-4
68-69	0.0	0.0	0.0	1.566505307220011	5.345978354133644E-4
70-71	0.0	0.0	0.0	1.6912002523301783	5.345978354133644E-4
72-73	0.0	0.0	0.0	1.7954468302357842	0.0012028451296800698
74-75	0.0	0.0	0.0	1.89408013086955	0.0014701440473867521
76-77	0.0	0.0	0.0	2.0078158203537435	0.0016037935062400933
78-79	0.0	0.0	0.0	2.1363865997706575	0.0016037935062400933
80-81	0.0	0.0	0.0	2.267363069446932	0.0016037935062400933
82-83	0.0	0.0	0.0	2.40569025936014	0.0016037935062400933
84-85	0.0	0.0	0.0	2.5373349763306807	0.0016037935062400933
86-87	0.0	0.0	0.0	2.6660394052064484	0.0018710924239467755
88-89	0.0	0.0	0.0	2.8078414810498433	0.0021383913416534576
90-91	0.0	0.0	0.0	2.9545885868708117	0.0021383913416534576
92-93	0.0	0.0	0.0	3.1094883096818338	0.0021383913416534576
94-95	0.0	0.0	0.0	3.281227864308377	0.00240569025936014
96-97	0.0	0.0	0.0	3.449492533004734	0.0025393397182134807
98-99	0.0	0.0	0.0	3.6307211992098645	0.002672989177066822
100-101	0.0	0.0	0.0	3.827720501559689	0.002672989177066822
102-103	0.0	0.0	0.0	4.016834485837167	0.002672989177066822
104-105	0.0	0.0	0.0	4.2177096224937385	0.002672989177066822
106-107	0.0	0.0	0.0	4.433687148000738	0.002672989177066822
108-109	0.0	0.0	0.0	4.646457086495257	0.002672989177066822
110-111	0.0	0.0	0.0	4.866043147391296	0.002672989177066822
112-113	0.0	0.0	0.0	5.093915474736242	0.002672989177066822
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTACTA	60	2.1967177E-5	49.559666	9
GGTATCA	1790	0.0	48.181755	1
GTATCAA	2600	0.0	37.060337	1
TATCAAC	3275	0.0	29.96279	2
CAACGCA	3415	0.0	28.908596	5
ATCAACG	3390	0.0	28.77092	3
TCAACGC	3535	0.0	27.927256	4
AACGCAG	3595	0.0	27.792013	6
TCCGACC	90	0.009543089	26.431822	8
ACGCAGA	3825	0.0	25.809896	7
CGCAGAG	3925	0.0	25.152319	8
CTGTACG	145	1.3420587E-4	24.608938	9
GAGTACT	1730	0.0	24.5793	12-13
GCAGAGT	4065	0.0	24.432367	9
TATTCTC	255	2.4796464E-8	23.322197	5
CAGAGTA	4115	0.0	22.76252	10-11
ATGGGGG	1235	0.0	21.188265	18-19
AGTACTT	1855	0.0	20.037601	12-13
GTACTTT	2130	0.0	19.96347	14-15
TCGGAGA	150	0.004713075	19.823866	2
>>END_MODULE