FastQCFastQC Report
Sun 1 Jan 2017
ERR1142557_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142557_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298969
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4990.16690693683960545No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4320.14449658660262435No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3320.11104830266683169No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA3050.10201726600416766No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA1051.4684612E-534.0389181
GGTATCA15500.033.819311
CGTTAAC750.003884059831.7696579
GTATTAG1203.6582504E-529.7840541
ACGTTAA800.00532603229.7840548
AGGGAGT1203.6582504E-529.7840546
TCTAACG900.00946491226.4747122
GTATCAA20100.025.783212
TATTCTC2001.8002811E-623.827243
TATCAAC23300.021.9865113
GGGCTAT2755.4147677E-821.6611296
ATCAACG22550.021.6611294
AACGCAG22450.021.492287
TAAAACG1400.003129407321.2743233
CAACGCA23050.021.1912566
GGTCTAA853.9224923E-421.00639756-57
TCAACGC23650.020.9055085
ATTCAAG1450.003835250420.5407281
ACGCAGA23550.020.4883928
CGCAGAG24050.020.3101239
Produced by FastQC (version 0.11.5)