##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142557_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	298969
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18480511357365	33.0	33.0	33.0	27.0	33.0
2	31.274001652345227	33.0	33.0	33.0	27.0	33.0
3	31.371536848301997	33.0	33.0	33.0	27.0	33.0
4	31.28393913750255	33.0	33.0	33.0	27.0	33.0
5	31.35185587803418	33.0	33.0	33.0	27.0	33.0
6	34.539567647481846	37.0	37.0	37.0	27.0	37.0
7	34.67967916406049	37.0	37.0	37.0	27.0	37.0
8	34.64930812224679	37.0	37.0	37.0	27.0	37.0
9	34.50898588147935	37.0	37.0	37.0	27.0	37.0
10-11	34.61723958002335	37.0	37.0	37.0	27.0	37.0
12-13	34.612538423716174	37.0	37.0	37.0	27.0	37.0
14-15	34.546029855938244	37.0	37.0	37.0	27.0	37.0
16-17	34.56346811876817	37.0	37.0	37.0	27.0	37.0
18-19	34.57479203529463	37.0	37.0	37.0	27.0	37.0
20-21	34.647311259695826	37.0	37.0	37.0	27.0	37.0
22-23	34.71248691335891	37.0	37.0	37.0	27.0	37.0
24-25	34.80958059196773	37.0	37.0	37.0	27.0	37.0
26-27	34.797311761420076	37.0	37.0	37.0	27.0	37.0
28-29	34.855496723740586	37.0	37.0	37.0	27.0	37.0
30-31	34.92865481036496	37.0	37.0	37.0	27.0	37.0
32-33	34.93773769186772	37.0	37.0	37.0	27.0	37.0
34-35	34.94725205623325	37.0	37.0	37.0	27.0	37.0
36-37	34.911691513166915	37.0	37.0	37.0	27.0	37.0
38-39	34.895738354143745	37.0	37.0	37.0	27.0	37.0
40-41	34.869051640805566	37.0	37.0	37.0	27.0	37.0
42-43	34.856652361950566	37.0	37.0	37.0	27.0	37.0
44-45	34.90381945954263	37.0	37.0	37.0	27.0	37.0
46-47	34.88920423187689	37.0	37.0	37.0	27.0	37.0
48-49	34.875642625155116	37.0	37.0	37.0	27.0	37.0
50-51	34.863168087661265	37.0	37.0	37.0	27.0	37.0
52-53	34.83024995902585	37.0	37.0	37.0	27.0	37.0
54-55	34.80814231575849	37.0	37.0	37.0	27.0	37.0
56-57	34.759235907401774	37.0	37.0	37.0	27.0	37.0
58-59	34.706676946439266	37.0	37.0	37.0	27.0	37.0
60-61	34.68591559660032	37.0	37.0	37.0	27.0	37.0
62-63	34.71138813723162	37.0	37.0	37.0	27.0	37.0
64-65	34.656173382524614	37.0	37.0	37.0	27.0	37.0
66-67	34.617482080081885	37.0	37.0	37.0	27.0	37.0
68-69	34.56039422147447	37.0	37.0	37.0	27.0	37.0
70-71	34.513335830805204	37.0	37.0	37.0	27.0	37.0
72-73	34.455492040980836	37.0	37.0	37.0	27.0	37.0
74-75	34.380181557285205	37.0	37.0	37.0	27.0	37.0
76-77	34.331579528312304	37.0	37.0	37.0	27.0	37.0
78-79	34.25391763025598	37.0	37.0	37.0	27.0	37.0
80-81	34.188578080001605	37.0	37.0	37.0	27.0	37.0
82-83	34.15339048530115	37.0	37.0	37.0	27.0	37.0
84-85	34.112001578759006	37.0	37.0	37.0	27.0	37.0
86-87	34.048354511671775	37.0	37.0	37.0	27.0	37.0
88-89	34.01466038284906	37.0	37.0	37.0	27.0	37.0
90-91	33.92666296505658	37.0	37.0	37.0	22.0	37.0
92-93	33.797440537313236	37.0	37.0	37.0	22.0	37.0
94-95	33.74616599045386	37.0	37.0	37.0	22.0	37.0
96-97	33.6430499483224	37.0	33.0	37.0	22.0	37.0
98-99	33.60999300930866	37.0	33.0	37.0	22.0	37.0
100-101	33.478691770718704	37.0	33.0	37.0	22.0	37.0
102-103	33.413681686061096	37.0	33.0	37.0	22.0	37.0
104-105	33.302128314306835	37.0	33.0	37.0	22.0	37.0
106-107	33.22627429599724	37.0	33.0	37.0	14.0	37.0
108-109	33.114508527639984	37.0	33.0	37.0	14.0	37.0
110-111	33.02861835173546	37.0	33.0	37.0	14.0	37.0
112-113	32.941786606638146	37.0	33.0	37.0	14.0	37.0
114-115	32.78180179215905	37.0	33.0	37.0	14.0	37.0
116-117	32.575882449350935	37.0	33.0	37.0	14.0	37.0
118-119	32.460020938625746	37.0	33.0	37.0	14.0	37.0
120-121	32.37783683258131	37.0	33.0	37.0	14.0	37.0
122-123	32.17171178282698	37.0	33.0	37.0	14.0	37.0
124-125	30.540540992544376	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	497.0
1	50.0
2	17.0
3	16.0
4	10.0
5	12.0
6	8.0
7	5.0
8	7.0
9	10.0
10	9.0
11	8.0
12	4.0
13	7.0
14	31.0
15	155.0
16	209.0
17	378.0
18	689.0
19	1220.0
20	1905.0
21	2504.0
22	2797.0
23	3021.0
24	3165.0
25	3385.0
26	3902.0
27	4649.0
28	5572.0
29	6571.0
30	7789.0
31	9509.0
32	12167.0
33	16049.0
34	24291.0
35	46224.0
36	142127.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.974865398300196	20.63736086682152	12.540783308046564	23.846990426831724
2	17.220926916819028	23.05818810070953	36.3664726504185	23.354412332052938
3	19.431446540880504	28.25861635220126	28.334758909853246	23.975178197064988
4	14.298748784005905	20.241521585991748	34.19610210995941	31.263627520042935
5	14.984499141077947	37.80833959630663	31.390581382864507	15.816579879750915
6	28.254077979080748	36.93184296802381	18.485719942416686	16.328359110478754
7	25.331476416598154	34.093326845311005	21.966541940030538	18.608654798060307
8	25.087248322147648	34.078859060402685	20.488590604026847	20.34530201342282
9	26.03029814881935	19.05380371310056	20.419368262789792	34.49652987529029
10-11	24.787530895063334	28.00330218518077	25.161711431299278	22.047455488456613
12-13	25.726012272615158	27.22711721068996	24.748767117311367	22.298103399383518
14-15	24.22994708035933	27.108144658203553	23.316856767975732	25.34505149346139
16-17	23.40990347399701	28.85449996310681	24.25006204763917	23.48553451525701
18-19	23.478778528667014	27.95700367246323	25.92624888903795	22.637968909831805
20-21	25.584643345762803	26.503440459264425	25.20304074898731	22.70887544598546
22-23	25.74241324331438	26.27756439165956	24.670937369544333	23.309084995481726
24-25	24.316137446399054	27.1744633200232	25.10787242388582	23.401526809691923
26-27	24.003795384517378	27.161977757437377	25.187841358291674	23.64638549975357
28-29	23.61992755384432	26.84681543662973	25.814264953124557	23.718992056401394
30-31	24.08977765113102	26.604758751896206	25.78358490407905	23.52187869289372
32-33	23.799929599892724	26.945138202115356	25.373371997519236	23.881560200472688
34-35	23.937390931173276	26.973800012405135	25.41444058693068	23.674368469490904
36-37	23.906491486273104	27.119882795868357	25.14792998595294	23.825695731905604
38-39	23.844271636388015	27.03710531608232	25.498257022189446	23.620366025340218
40-41	23.426386451224644	26.960259227781492	25.71709237085129	23.896261950142573
42-43	23.801350633329367	26.939754512586973	25.510175005740777	23.748719848342883
44-45	23.901934592894147	27.184356998841892	25.261328421571307	23.652379986692658
46-47	23.738992266512017	27.107769356347195	25.290718138331215	23.862520238809573
48-49	23.892509016800076	27.05459018811257	25.457208747301664	23.59569204778569
50-51	23.3347579421944	27.39154117538611	25.39608316363728	23.87761771878221
52-53	23.473218132771805	27.176524434713066	25.1899937465107	24.160263686004424
54-55	23.486616242892396	27.16463496405965	25.12052757214891	24.228221220899044
56-57	23.766545522818404	26.85202569501254	25.120026285085896	24.26140249708316
58-59	23.591433609805488	26.909769949242662	25.517128086843993	23.981668354107857
60-61	23.604686837256633	27.28897412985202	25.423359414162107	23.682979618729245
62-63	23.419352135104553	27.188702174915143	25.33730042324938	24.054645266730923
64-65	23.241903957245786	26.91517218025702	25.418841760459728	24.424082102037463
66-67	23.812158030254267	26.700293691663983	25.24608411114687	24.24146416693488
68-69	23.6569016756986	26.73759233774789	25.190626010959004	24.414879975594506
70-71	23.443628683495927	26.964866405176167	25.420731502899862	24.17077340842804
72-73	23.98060953900721	26.70865027774891	25.653975480319552	23.656764702924328
74-75	24.163892771783036	26.760962332015943	25.358930265455875	23.716214630745146
76-77	23.796433803440152	26.65931391446894	25.53243200659736	24.011820275493545
78-79	23.599530634481603	26.86648227307015	25.50565753080211	24.02832956164613
80-81	23.360062218408068	26.933597940356147	25.709846331259385	23.9964935099764
82-83	23.792017863249583	26.559346355266776	25.826443510155368	23.822192271328277
84-85	23.91644654804843	26.726646196752522	25.324311822045832	24.03259543315322
86-87	23.605508415634997	26.83299619398445	25.559128990576298	24.002366399804252
88-89	24.018644127451818	26.32636608793445	25.53025313039995	24.124736654213784
90-91	24.00620743283102	26.465047996996837	25.562255322571996	23.96648924760015
92-93	23.612137791388623	27.017028644798796	25.62024060357067	23.75059296024191
94-95	22.842394583361266	26.809680230609374	26.01092713012	24.336998055909365
96-97	23.300106918892467	26.886749766219666	25.560989016513773	24.252154298374094
98-99	23.121741796994787	27.377032423256804	25.345069985907305	24.156155793841105
100-101	23.332557005631447	27.574322207272793	25.140115980007604	23.953004807088153
102-103	23.448533576424616	27.642863606352996	25.419516789050167	23.489086028172224
104-105	23.272013727369615	27.815350791937853	25.44322378695766	23.469411693734877
106-107	23.12669141119201	27.68627516694176	25.537020435179674	23.65001298668655
108-109	23.18947922287277	27.733166656053836	25.358015423234054	23.71933869783934
110-111	22.960697738716622	28.340021950953858	25.181430498420703	23.51784981190881
112-113	22.932948162844006	28.343208353999383	24.934818161101354	23.789025322055256
114-115	22.976145196449846	28.319808853270423	25.09554823154089	23.608497718738846
116-117	23.233866859701333	28.172839754417005	24.949898120593044	23.643395265288614
118-119	22.942508862574805	27.94793698022423	25.063663108233985	24.045891048966983
120-121	23.035654446752897	28.020239930299578	25.386870853159976	23.557234769787545
122-123	22.825970405318465	28.35386285653013	25.47883604975338	23.341330688398028
124-125	22.999971518515476	28.2306799200508	25.189527525679413	23.579821035754314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	689.0
1	425.0
2	144.5
3	106.0
4	72.5
5	59.0
6	55.5
7	50.5
8	51.5
9	56.5
10	56.0
11	58.0
12	60.0
13	66.5
14	82.5
15	96.5
16	103.5
17	113.0
18	145.5
19	181.0
20	202.5
21	249.0
22	321.0
23	388.0
24	459.5
25	562.0
26	716.5
27	910.0
28	1124.5
29	1558.0
30	2309.0
31	2925.0
32	3598.0
33	4394.0
34	5059.5
35	5685.5
36	6328.5
37	7280.0
38	7609.5
39	7402.0
40	7305.5
41	7158.0
42	7347.5
43	7967.0
44	8804.0
45	9784.0
46	10913.5
47	11871.5
48	12686.5
49	13206.0
50	13315.5
51	12896.5
52	12203.0
53	12007.5
54	11802.5
55	11181.0
56	10435.0
57	9199.5
58	8117.5
59	7322.0
60	6295.0
61	5359.0
62	4449.0
63	3504.0
64	2633.5
65	1949.0
66	1448.5
67	1128.0
68	910.0
69	723.0
70	636.0
71	574.5
72	473.5
73	455.5
74	384.5
75	303.5
76	240.5
77	161.0
78	122.0
79	79.0
80	54.5
81	49.0
82	33.5
83	23.0
84	17.5
85	10.5
86	8.0
87	4.5
88	2.5
89	2.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3522104298438969
2	0.29568282999240725
3	0.28230351641809015
4	0.28732075900845905
5	0.3080586950486505
6	0.3231104228197572
7	0.3291311139281999
8	0.32411387133783104
9	0.33347939083985295
10-11	0.3292983553478789
12-13	0.3306362867053106
14-15	0.3244483541771889
16-17	0.2712655827192786
18-19	0.2689242028437731
20-21	0.2518655780365189
22-23	0.24601212834775513
24-25	0.23413798755054874
26-27	0.2381517816228438
28-29	0.2269466065043533
30-31	0.22627764082563742
32-33	0.22544143372724262
34-35	0.23597764316701733
36-37	0.22945522779953775
38-39	0.21038970595613593
40-41	0.23363626329151183
42-43	0.22226384675334232
44-45	0.21323281009067832
46-47	0.21942074261879996
48-49	0.21306556867099935
50-51	0.21457074144811003
52-53	0.24601212834775513
54-55	0.23313453903247494
56-57	0.23447247038990665
58-59	0.2297897106388957
60-61	0.24400523131160753
62-63	0.2279500550224271
64-65	0.23614488458669627
66-67	0.23313453903247494
68-69	0.2259431579862795
70-71	0.22711384792403225
72-73	0.22677936508467433
74-75	0.21808281126136825
76-77	0.2234345366910951
78-79	0.23213109051440114
80-81	0.2224310881730213
82-83	0.23514143606862248
84-85	0.21574143138586274
86-87	0.20921901601838316
88-89	0.21557418996618377
90-91	0.2063759118838408
92-93	0.2266121236649954
94-95	0.21038970595613593
96-97	0.20470349768705118
98-99	0.19650866812278198
100-101	0.1858052172633283
102-103	0.19734487522117677
104-105	0.19634142670310298
106-107	0.196174185283424
108-109	0.19667590954246095
110-111	0.19282935688984476
112-113	0.19165866695209202
114-115	0.18714314862076
116-117	0.19299659830952373
118-119	0.17493452498419568
120-121	0.1836310788075018
122-123	0.17961728473520666
124-125	0.1771086634400222
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	298969.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.7290239194068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.3831918164268	58.50649264769476
2	8.531594081129743	11.556730791830116
3	2.0387622672639454	4.142501350965114
4	0.8134417832193388	2.2037447197083013
5	0.4401153380194675	1.4904291128009162
6	0.29325434297093755	1.1917098245729134
7	0.20739162054532045	0.9832502420018985
8	0.14676401236166067	0.7952146643000826
9	0.14484050602424506	0.8828915487313571
>10	0.8999490165394157	11.934878927148887
>50	0.07362660918214924	3.312461158408316
>100	0.02706860631696663	2.99969501183733
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	499	0.16690693683960545	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	432	0.14449658660262435	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	332	0.11104830266683169	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	305	0.10201726600416766	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.3448283935792674E-4	0.0
2	0.0	0.0	0.0	3.3448283935792674E-4	0.0
3	0.0	0.0	0.0	3.3448283935792674E-4	0.0
4	0.0	0.0	0.0	3.3448283935792674E-4	0.0
5	0.0	0.0	0.0	3.3448283935792674E-4	0.0
6	0.0	0.0	0.0	3.3448283935792674E-4	3.3448283935792674E-4
7	0.0	0.0	0.0	3.3448283935792674E-4	3.3448283935792674E-4
8	0.0	0.0	0.0	3.3448283935792674E-4	3.3448283935792674E-4
9	0.0	0.0	0.0	3.3448283935792674E-4	3.3448283935792674E-4
10-11	0.0	0.0	0.0	3.3448283935792674E-4	3.3448283935792674E-4
12-13	0.0	0.0	0.0	5.017242590368901E-4	3.3448283935792674E-4
14-15	0.0	0.0	0.0	0.0010034485180737802	3.3448283935792674E-4
16-17	0.0	0.0	0.0	0.0011706899377527434	3.3448283935792674E-4
18-19	0.0	0.0	0.0	0.001337931357431707	3.3448283935792674E-4
20-21	0.0	0.0	0.0	0.001839655616468597	3.3448283935792674E-4
22-23	0.0	0.0	0.0	0.003679311232937194	3.3448283935792674E-4
24-25	0.0	0.0	0.0	0.008529312403627132	3.3448283935792674E-4
26-27	0.0	0.0	0.0	0.016222417708859446	3.3448283935792674E-4
28-29	0.0	0.0	0.0	0.02893276560446066	3.3448283935792674E-4
30-31	0.0	0.0	0.0	0.05134311584144176	3.3448283935792674E-4
32-33	0.0	0.0	0.0	0.09031036662664021	3.3448283935792674E-4
34-35	0.0	0.0	0.0	0.1337931357431707	3.3448283935792674E-4
36-37	0.0	0.0	0.0	0.1921603912111289	3.3448283935792674E-4
38-39	0.0	0.0	0.0	0.27929317086386884	3.3448283935792674E-4
40-41	0.0	0.0	0.0	0.3722794002053724	3.3448283935792674E-4
42-43	0.0	0.0	0.0	0.44034665801471057	3.3448283935792674E-4
44-45	0.0	0.0	0.0	0.5232984021754764	3.3448283935792674E-4
46-47	0.0	0.0	0.0	0.6214691155270279	3.3448283935792674E-4
48-49	0.0	0.0	0.0	0.7164622419046791	3.3448283935792674E-4
50-51	0.0	0.0	0.0	0.7959019162521868	3.3448283935792674E-4
52-53	0.0	0.0	0.0	0.871996762206115	3.3448283935792674E-4
54-55	0.0	0.0	0.0	0.9477571253206853	3.3448283935792674E-4
56-57	0.0	0.0	0.0	1.0387364576260416	3.3448283935792674E-4
58-59	0.0	0.0	0.0	1.1425933792466778	3.3448283935792674E-4
60-61	0.0	0.0	0.0	1.241600299696624	3.3448283935792674E-4
62-63	0.0	0.0	0.0	1.3299037692871167	3.3448283935792674E-4
64-65	0.0	0.0	0.0	1.424395171405731	3.3448283935792674E-4
66-67	0.0	0.0	0.0	1.5193882977833821	3.3448283935792674E-4
68-69	0.0	0.0	0.0	1.638798671434162	3.3448283935792674E-4
70-71	0.0	0.0	0.0	1.7557004237897575	3.3448283935792674E-4
72-73	0.0	0.0	0.0	1.859055621151357	3.3448283935792674E-4
74-75	0.0	0.0	0.0	1.9749539249888786	3.3448283935792674E-4
76-77	0.0	0.0	0.0	2.0898487803083263	3.3448283935792674E-4
78-79	0.0	0.0	0.0	2.2122694995133276	3.3448283935792674E-4
80-81	0.0	0.0	0.0	2.3572678103749887	3.3448283935792674E-4
82-83	0.0	0.0	0.0	2.510962675059956	3.3448283935792674E-4
84-85	0.0	0.0	0.0	2.6449230522228055	3.3448283935792674E-4
86-87	0.0	0.0	0.0	2.7690161856245967	3.3448283935792674E-4
88-89	0.0	0.0	0.0	2.9148507035846527	3.3448283935792674E-4
90-91	0.0	0.0	0.0	3.0792490191290733	3.3448283935792674E-4
92-93	0.0	0.0	0.0	3.2439818175128523	3.3448283935792674E-4
94-95	0.0	0.0	0.0	3.396171509420709	3.3448283935792674E-4
96-97	0.0	0.0	0.0	3.5512043054631084	3.3448283935792674E-4
98-99	0.0	0.0	0.0	3.7227940020537247	3.3448283935792674E-4
100-101	0.0	0.0	0.0	3.9152888761042113	3.3448283935792674E-4
102-103	0.0	0.0	0.0	4.101763059046256	3.3448283935792674E-4
104-105	0.0	0.0	0.0	4.2850596550144	3.3448283935792674E-4
106-107	0.0	0.0	0.0	4.46735280246447	3.3448283935792674E-4
108-109	0.0	0.0	0.0	4.680585612555149	3.3448283935792674E-4
110-111	0.0	0.0	0.0	4.891477042770321	3.3448283935792674E-4
112-113	0.0	0.0	0.0	5.127454685937337	3.3448283935792674E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATA	105	1.4684612E-5	34.038918	1
GGTATCA	1550	0.0	33.81931	1
CGTTAAC	75	0.0038840598	31.769657	9
GTATTAG	120	3.6582504E-5	29.784054	1
ACGTTAA	80	0.005326032	29.784054	8
AGGGAGT	120	3.6582504E-5	29.784054	6
TCTAACG	90	0.009464912	26.474712	2
GTATCAA	2010	0.0	25.78321	2
TATTCTC	200	1.8002811E-6	23.82724	3
TATCAAC	2330	0.0	21.986511	3
GGGCTAT	275	5.4147677E-8	21.661129	6
ATCAACG	2255	0.0	21.661129	4
AACGCAG	2245	0.0	21.49228	7
TAAAACG	140	0.0031294073	21.274323	3
CAACGCA	2305	0.0	21.191256	6
GGTCTAA	85	3.9224923E-4	21.006397	56-57
TCAACGC	2365	0.0	20.905508	5
ATTCAAG	145	0.0038352504	20.540728	1
ACGCAGA	2355	0.0	20.488392	8
CGCAGAG	2405	0.0	20.310123	9
>>END_MODULE