FastQCFastQC Report
Sun 1 Jan 2017
ERR1142557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298969
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17860.5973863510932572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17030.5696242754265493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.32076904294425174No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.168913833875753No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3690.12342416772307496No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3010.10067933464673595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.039.1195341
GTATCAA25150.030.9821131
GTGCTAT1004.439192E-429.7404651
ATGGTAC900.00954056526.4315433
GTGTTAT1150.001004947525.8612731
TATCAAC31050.024.7077482
GTGTAGG1701.3977313E-524.4921461
CAACGCA31900.024.235825
ATCAACG31800.023.9380043
AACGCAG33550.023.3945056
TCAACGC33700.022.9413264
GAGTACT16050.022.04318612-13
ATGTTAC1400.003156128321.2431891
ACGCAGA36550.021.1489287
CGCAGAG36250.020.9958958
CGGTATT1004.7970498E-520.88471482-83
GAATTCG1601.2230885E-820.4397324-25
GACTCGT1754.7931666E-420.386647
GCAGAGT38300.019.8720939
CAGAGTA38300.019.71684310-11
Produced by FastQC (version 0.11.5)