##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142557_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	298969
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.85820603473939	33.0	27.0	33.0	14.0	33.0
2	30.6145018379832	33.0	27.0	33.0	27.0	33.0
3	30.23001715896966	33.0	27.0	33.0	27.0	33.0
4	31.132271238824092	33.0	33.0	33.0	27.0	33.0
5	31.896106285267035	33.0	33.0	33.0	27.0	33.0
6	34.320802491228186	37.0	33.0	37.0	27.0	37.0
7	35.57337048322736	37.0	37.0	37.0	33.0	37.0
8	35.35666908609254	37.0	37.0	37.0	33.0	37.0
9	35.85343296462175	37.0	37.0	37.0	33.0	37.0
10-11	35.9190952908161	37.0	37.0	37.0	33.0	37.0
12-13	36.001577086587574	37.0	37.0	37.0	33.0	37.0
14-15	36.043982820961375	37.0	37.0	37.0	37.0	37.0
16-17	36.105256732303346	37.0	37.0	37.0	37.0	37.0
18-19	36.13159725590279	37.0	37.0	37.0	37.0	37.0
20-21	36.1547685545993	37.0	37.0	37.0	37.0	37.0
22-23	36.15725041726734	37.0	37.0	37.0	37.0	37.0
24-25	36.15887934869501	37.0	37.0	37.0	37.0	37.0
26-27	36.10665821540026	37.0	37.0	37.0	37.0	37.0
28-29	36.083164140763756	37.0	37.0	37.0	37.0	37.0
30-31	36.0393318370801	37.0	37.0	37.0	37.0	37.0
32-33	35.97888242593714	37.0	37.0	37.0	37.0	37.0
34-35	35.8453317902525	37.0	37.0	37.0	35.0	37.0
36-37	35.74304861039104	37.0	37.0	37.0	33.0	37.0
38-39	35.71428475862046	37.0	37.0	37.0	33.0	37.0
40-41	35.592168418799275	37.0	37.0	37.0	33.0	37.0
42-43	35.5357879913971	37.0	37.0	37.0	33.0	37.0
44-45	35.41505808294505	37.0	37.0	37.0	33.0	37.0
46-47	35.25268004375035	37.0	37.0	37.0	33.0	37.0
48-49	35.06984168927213	37.0	37.0	37.0	33.0	37.0
50-51	34.961086266469096	37.0	37.0	37.0	30.0	37.0
52-53	34.86779900257217	37.0	37.0	37.0	27.0	37.0
54-55	34.812575885794175	37.0	37.0	37.0	27.0	37.0
56-57	34.7991781756637	37.0	37.0	37.0	27.0	37.0
58-59	34.75721730346625	37.0	37.0	37.0	27.0	37.0
60-61	34.71586017279384	37.0	37.0	37.0	27.0	37.0
62-63	34.70979098167369	37.0	37.0	37.0	27.0	37.0
64-65	34.682351681946955	37.0	37.0	37.0	27.0	37.0
66-67	34.651079208881185	37.0	37.0	37.0	27.0	37.0
68-69	34.63377139435861	37.0	37.0	37.0	27.0	37.0
70-71	34.52195712598965	37.0	37.0	37.0	27.0	37.0
72-73	34.528099903334464	37.0	37.0	37.0	27.0	37.0
74-75	34.463136646274364	37.0	37.0	37.0	27.0	37.0
76-77	34.33274185617907	37.0	37.0	37.0	27.0	37.0
78-79	34.35060156738659	37.0	37.0	37.0	27.0	37.0
80-81	34.34311751385595	37.0	37.0	37.0	27.0	37.0
82-83	34.27378925574223	37.0	37.0	37.0	27.0	37.0
84-85	34.203102997300725	37.0	37.0	37.0	27.0	37.0
86-87	34.06418558445859	37.0	37.0	37.0	27.0	37.0
88-89	33.96818231990608	37.0	37.0	37.0	24.5	37.0
90-91	33.9329545872649	37.0	37.0	37.0	22.0	37.0
92-93	33.76634366773813	37.0	37.0	37.0	22.0	37.0
94-95	33.83133535583957	37.0	37.0	37.0	22.0	37.0
96-97	33.8439236174988	37.0	37.0	37.0	22.0	37.0
98-99	33.803173573179826	37.0	37.0	37.0	22.0	37.0
100-101	33.69482287461242	37.0	35.0	37.0	22.0	37.0
102-103	33.61291806173884	37.0	33.0	37.0	22.0	37.0
104-105	33.47456926972362	37.0	33.0	37.0	18.0	37.0
106-107	33.435071863638036	37.0	33.0	37.0	14.0	37.0
108-109	33.336963029611766	37.0	33.0	37.0	14.0	37.0
110-111	33.27849710170619	37.0	33.0	37.0	14.0	37.0
112-113	33.14415875893487	37.0	33.0	37.0	14.0	37.0
114-115	33.101998869447996	37.0	33.0	37.0	14.0	37.0
116-117	32.9598888179042	37.0	33.0	37.0	14.0	37.0
118-119	32.85280079205536	37.0	33.0	37.0	14.0	37.0
120-121	32.74150329967321	37.0	33.0	37.0	14.0	37.0
122-123	32.67673571507414	37.0	33.0	37.0	14.0	37.0
124-125	31.339876375142573	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	1.0
17	8.0
18	32.0
19	207.0
20	843.0
21	2323.0
22	3672.0
23	4742.0
24	3835.0
25	2585.0
26	2369.0
27	2697.0
28	3232.0
29	4363.0
30	5846.0
31	7781.0
32	11015.0
33	16493.0
34	27023.0
35	55582.0
36	144318.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.9845699870859	18.010491873481925	13.017484225387923	24.987453914044256
2	17.837969822958232	20.961370576882555	37.52228491917222	23.67837468098699
3	20.26618411458159	25.74908183539265	29.242121177658998	24.742612872366756
4	14.508052782098238	18.10713628685632	35.59747796564815	31.787332965397287
5	15.696250802825947	34.04517233997002	32.92656818668379	17.33200867052023
6	28.458226992818464	35.3179846200675	18.939594127662136	17.2841942594519
7	24.962788784121432	32.45386645438156	23.45460566145654	19.128739100040473
8	25.64814412196582	31.39690068201051	21.438343105806958	21.51661209021671
9	26.63085470399941	16.035441801658365	21.674153507554294	35.65954998678792
10-11	25.258471614113837	25.353631981911168	26.58837538340095	22.79952102057404
12-13	26.712178707790287	24.657112705853628	26.598119868815612	22.032588717540477
14-15	24.83551806374574	24.472938665881745	25.88244935093605	24.809093919436464
16-17	22.782295154347107	26.62667366850744	27.014506520742955	23.576524656402505
18-19	22.7060330669735	24.85040255009717	30.076362432225412	22.36720195070392
20-21	24.346251103797062	23.868273527601616	29.676085734928154	22.109389633673167
22-23	24.280778274670617	23.82721954450127	29.372777779636017	22.519224401192098
24-25	23.252243543644994	24.666102505610947	29.60908990564239	22.472564045101667
26-27	22.704026169937354	24.891711180757873	29.722814071024082	22.68144857828069
28-29	22.43070212701906	24.53932426420661	30.315122607145366	22.714851001628965
30-31	22.878869979395787	24.321666532873085	30.181490995691846	22.617972492039282
32-33	22.684739980098172	24.574744746502546	29.68583541689314	23.05467985650614
34-35	22.800357227672436	24.55789730707866	29.803257193889664	22.83848827135924
36-37	23.02941309281506	24.57056081721804	29.47029005018974	22.929736039777165
38-39	22.929300362244913	24.540504199432046	29.66578474691356	22.86441069140948
40-41	22.540786887987625	24.392189655893297	29.952753271731403	23.114270184387674
42-43	23.12815152402057	24.3291382698499	29.355186687293553	23.187523518835977
44-45	23.035331422321377	24.742531834404236	28.947148366553055	23.274988376721332
46-47	22.994022791660672	24.702393893681286	28.798303503038774	23.505279811619264
48-49	23.32566252688406	24.79354046740632	28.631062083359815	23.24973492234981
50-51	23.097210747602595	24.980181891768044	28.33169994213447	23.590907418494893
52-53	22.842836548270892	25.10912502634052	27.990360204569704	24.057678220818882
54-55	22.967264164512038	25.194250908957116	27.759901528252094	24.078583398278752
56-57	23.216621121253375	24.93251808715954	27.51991009101278	24.33095070057431
58-59	22.985660720676726	25.078519846539272	27.84619810080644	24.08962133197756
60-61	23.021115901648667	25.216995742033454	28.14773438048761	23.61415397583027
62-63	22.8864865588071	25.15611986527031	27.946542952613818	24.010850623308773
64-65	22.65402098545334	24.961618094183677	28.039696423375	24.344664496987985
66-67	23.1353417912894	24.760593907729564	28.088363676501576	24.01570062447946
68-69	22.968936578708828	24.57896972595821	28.143720586415316	24.308373108917646
70-71	22.735634798256676	24.823811164368212	28.460308593867587	23.98024544350752
72-73	23.147550414925963	24.694366305536693	28.589586211279432	23.568497068257912
74-75	23.10662807806325	24.818006973400312	28.480130161211758	23.59523478732468
76-77	22.721457945427513	24.859014373166517	28.59630557378806	23.823222107617912
78-79	22.700217581450886	24.85930792039964	28.503839033209182	23.936635464940288
80-81	22.401486441738104	25.103606059491117	28.585405175787454	23.909502322983318
82-83	22.818084818158404	24.798223227157333	28.80415695272754	23.579535001956724
84-85	22.91684801896331	24.952290395805573	28.426051787519835	23.704809797711277
86-87	22.62114640800649	25.154177196522298	28.383351544118142	23.841324851353072
88-89	23.194300765721433	24.53410369346999	28.374782665555614	23.896812875252962
90-91	22.988663551417517	24.669458224575866	28.42600669371163	23.91587153029499
92-93	22.918917832246912	24.99644001722194	28.28536151834522	23.79928063218593
94-95	22.11407240908726	24.9735757673062	28.566936929704852	24.345414893901687
96-97	22.571069241292577	24.79772150289829	28.23754302285521	24.39366623295392
98-99	22.495308878178005	25.275195756081736	27.754382561402686	24.475112804337574
100-101	22.534108887543525	25.31817680093923	27.65838598650696	24.489328325010284
102-103	22.62692787546535	25.242750920664015	27.665075643294117	24.465245560576516
104-105	22.805550491780796	25.50068653636134	27.57434411109011	24.119418860767748
106-107	22.63328304941315	25.450464763905288	27.392639370637088	24.523612816044473
108-109	22.751971368125297	25.691421308336192	27.02651626012861	24.530091063409902
110-111	22.472731286521345	26.08531319300663	27.006144449759006	24.43581107071302
112-113	22.43225886295904	25.970585579106864	26.845090962608165	24.752064595325937
114-115	22.48795025571213	25.91138211654051	27.009489278152582	24.591178349594774
116-117	22.587032725910465	25.913642931684993	26.793001257659682	24.706323084744856
118-119	22.2250467439768	25.6514889503594	27.161177245801404	24.962287059862394
120-121	22.359341603979008	25.691459649662672	27.462546284062896	24.48665246229542
122-123	22.366198502185846	25.965568336516494	27.491646291087037	24.176586870210624
124-125	22.250616288754276	25.738626671171065	27.591506754926133	24.41925028514853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	61.5
2	121.5
3	174.0
4	155.5
5	144.0
6	197.0
7	387.0
8	625.0
9	746.5
10	724.0
11	675.0
12	635.5
13	611.0
14	605.5
15	615.0
16	623.5
17	624.0
18	642.0
19	620.0
20	608.0
21	643.5
22	640.0
23	637.5
24	688.0
25	753.0
26	830.0
27	962.0
28	1122.0
29	1408.5
30	2025.5
31	2592.0
32	3206.0
33	3961.0
34	4592.0
35	5296.0
36	5876.0
37	6654.5
38	7194.0
39	7091.0
40	6928.5
41	6790.5
42	6900.0
43	7448.0
44	8349.5
45	9389.5
46	10555.5
47	11654.5
48	12439.0
49	12909.5
50	13113.0
51	12757.0
52	12037.5
53	11714.0
54	11632.5
55	11216.0
56	10461.5
57	9250.5
58	8162.5
59	7450.0
60	6551.5
61	5412.5
62	4379.5
63	3479.0
64	2572.0
65	1901.0
66	1437.0
67	1103.5
68	868.0
69	735.5
70	652.0
71	563.0
72	471.0
73	437.0
74	359.5
75	304.0
76	255.0
77	158.5
78	105.5
79	74.5
80	55.5
81	38.5
82	36.0
83	29.0
84	16.0
85	9.0
86	5.5
87	3.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023748281594412796
2	0.0
3	0.0010034485180737802
4	0.001337931357431707
5	0.008362070983948169
6	0.002675862714863414
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.6724141967896337E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.3448283935792674E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0020068970361475603
30-31	3.3448283935792674E-4
32-33	5.017242590368901E-4
34-35	0.0
36-37	0.0011706899377527437
38-39	0.0
40-41	0.0021741384558265236
42-43	0.0021741384558265236
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.08780174533145577
76-77	0.11941037365077985
78-79	1.6724141967896337E-4
80-81	0.0
82-83	0.0
84-85	0.09599657489572498
86-87	0.20436901484769324
88-89	0.2520328194561978
90-91	0.11054657840779479
92-93	0.17142245517093743
94-95	3.3448283935792674E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0015051727771106705
106-107	0.0
108-109	5.017242590368901E-4
110-111	0.0
112-113	0.0
114-115	0.0
116-117	3.3448283935792674E-4
118-119	0.0
120-121	0.0
122-123	0.0
124-125	6.689656787158535E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	298969.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.36858402196203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.54913851828395	53.57759188180382
2	9.664448684051846	12.248448569225594
3	2.2589684509148262	4.294428962542727
4	0.949832038371222	2.4075804532111293
5	0.510189114808146	1.6164980894405216
6	0.35738247250810806	1.3588092742263949
7	0.23064046619154238	1.023075183250627
8	0.17103295650896452	0.8670493020050317
9	0.14965432330484113	0.8535044304533425
>10	1.0411996034457212	13.108386150431347
>50	0.08328617599603555	3.599337544653673
>100	0.03194538257382185	3.254839334343846
>500	0.0011409065204936377	0.5292186567613716
>1k	0.0011409065204936377	1.261232167650564
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1786	0.5973863510932572	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1703	0.5696242754265493	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	959	0.32076904294425174	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	505	0.168913833875753	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	369	0.12342416772307496	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	301	0.10067933464673595	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.3448283935792674E-4	0.0
2	0.0	0.0	0.0	3.3448283935792674E-4	0.0
3	0.0	0.0	0.0	3.3448283935792674E-4	0.0
4	0.0	0.0	0.0	3.3448283935792674E-4	0.0
5	0.0	0.0	0.0	3.3448283935792674E-4	0.0
6	0.0	0.0	0.0	3.3448283935792674E-4	0.0
7	0.0	0.0	0.0	3.3448283935792674E-4	0.0
8	0.0	0.0	0.0	3.3448283935792674E-4	0.0
9	0.0	0.0	0.0	3.3448283935792674E-4	0.0
10-11	0.0	0.0	0.0	3.3448283935792674E-4	0.0
12-13	0.0	0.0	0.0	5.017242590368901E-4	0.0
14-15	0.0	0.0	0.0	0.0010034485180737802	0.0
16-17	0.0	0.0	0.0	0.0011706899377527434	0.0
18-19	0.0	0.0	0.0	0.001337931357431707	0.0
20-21	0.0	0.0	0.0	0.001839655616468597	0.0
22-23	0.0	0.0	0.0	0.0038465526526161578	0.0
24-25	0.0	0.0	0.0	0.008529312403627132	0.0
26-27	0.0	0.0	0.0	0.016222417708859446	0.0
28-29	0.0	0.0	0.0	0.028765524184781698	0.0
30-31	0.0	0.0	0.0	0.0506741501627259	0.0
32-33	0.0	0.0	0.0	0.0894741595282454	0.0
34-35	0.0	0.0	0.0	0.13262244580541793	0.0
36-37	0.0	0.0	0.0	0.19048797701433928	0.0
38-39	0.0	0.0	0.0	0.2769517909883633	0.0
40-41	0.0	0.0	0.0	0.37010526174954594	0.0
42-43	0.0	0.0	0.0	0.43767079529984715	0.0
44-45	0.0	0.0	0.0	0.5191173666835023	0.0
46-47	0.0	0.0	0.0	0.6152811829989062	0.0
48-49	0.0	0.0	0.0	0.7102743093765574	0.0
50-51	0.0	0.0	0.0	0.7888777766256703	0.0
52-53	0.0	0.0	0.0	0.8663105539370303	0.0
54-55	0.0	0.0	0.0	0.9430743655696745	0.0
56-57	0.0	0.0	0.0	1.036060594911178	0.0
58-59	0.0	0.0	0.0	1.1427606206663565	0.0
60-61	0.0	0.0	0.0	1.2432727138934139	0.0
62-63	0.0	0.0	0.0	1.3320779077429434	0.0
64-65	0.0	0.0	0.0	1.4259003441828417	0.0
66-67	0.0	0.0	0.0	1.5208934705604928	0.0
68-69	0.0	0.0	0.0	1.6399693613719148	0.0
70-71	0.0	0.0	0.0	1.7550314581110418	0.0
72-73	0.0	0.0	0.0	1.8582194140529622	0.0
74-75	0.0	0.0	0.0	1.9751211664085573	0.0
76-77	0.0	0.0	0.0	2.0888453317902522	0.0
78-79	0.0	0.0	0.0	2.210764326736217	0.0
80-81	0.0	0.0	0.0	2.356933327535631	0.0
82-83	0.0	0.0	0.0	2.510962675059956	0.0
84-85	0.0	0.0	0.0	2.6450902936424847	0.0
86-87	0.0	0.0	0.0	2.770521358401707	0.0
88-89	0.0	0.0	0.0	2.9153524278436898	0.0
90-91	0.0	0.0	0.0	3.0787472948700367	0.0
92-93	0.0	0.0	0.0	3.2404697476995943	0.0
94-95	0.0	0.0	0.0	3.392659439607451	0.0
96-97	0.0	0.0	0.0	3.5460198214530605	0.0
98-99	0.0	0.0	0.0	3.7199508979191824	0.0
100-101	0.0	0.0	0.0	3.9147871518451742	0.0
102-103	0.0	0.0	0.0	4.101428576206898	0.0
104-105	0.0	0.0	0.0	4.282718275138894	0.0
106-107	0.0	0.0	0.0	4.465345905428322	0.0
108-109	0.0	0.0	0.0	4.679582164037074	0.0
110-111	0.0	0.0	0.0	4.88829945579642	0.0
112-113	0.0	0.0	0.0	5.122771926186327	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1855	0.0	39.119534	1
GTATCAA	2515	0.0	30.982113	1
GTGCTAT	100	4.439192E-4	29.740465	1
ATGGTAC	90	0.009540565	26.431543	3
GTGTTAT	115	0.0010049475	25.861273	1
TATCAAC	3105	0.0	24.707748	2
GTGTAGG	170	1.3977313E-5	24.492146	1
CAACGCA	3190	0.0	24.23582	5
ATCAACG	3180	0.0	23.938004	3
AACGCAG	3355	0.0	23.394505	6
TCAACGC	3370	0.0	22.941326	4
GAGTACT	1605	0.0	22.043186	12-13
ATGTTAC	140	0.0031561283	21.243189	1
ACGCAGA	3655	0.0	21.148928	7
CGCAGAG	3625	0.0	20.995895	8
CGGTATT	100	4.7970498E-5	20.884714	82-83
GAATTCG	160	1.2230885E-8	20.43973	24-25
GACTCGT	175	4.7931666E-4	20.38664	7
GCAGAGT	3830	0.0	19.872093	9
CAGAGTA	3830	0.0	19.716843	10-11
>>END_MODULE