FastQCFastQC Report
Sun 1 Jan 2017
ERR1142556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458316
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26950.5880222379319072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23630.5155831347803699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14910.3253213939727175No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.15251485874374884No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.13309594253746324No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5710.12458652981785492No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA602.1967531E-549.562514
GTATCAA39850.034.0320741
GGTATCA29050.032.965651
TATAACG850.00722070727.988242
TATCAAC49100.027.4965952
ATCAACG49900.026.9365773
TCAACGC51150.026.5108574
CAACGCA51000.026.4722125
AACGCAG52650.025.9758196
GTATAAC1451.3411859E-424.6130331
ACGCAGA56300.023.9749227
GAGTACT24800.023.85674312-13
CGCAGAG57300.023.5565138
GCAGAGT60800.022.3960639
TTACCCT1602.6153645E-422.3031314
ACCGACC1602.6192298E-422.2982648
CCTATAC1350.002559194322.0277823
GTACTTT27150.021.5727814-15
CATAGGG1400.003160490221.2410762
AGTACTT25400.020.71807112-13
Produced by FastQC (version 0.11.5)