##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142556_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458316
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.32172343972281	33.0	14.0	33.0	14.0	33.0
2	30.435260824409358	33.0	27.0	33.0	27.0	33.0
3	30.00750137459744	33.0	27.0	33.0	27.0	33.0
4	31.04263870342733	33.0	33.0	33.0	27.0	33.0
5	31.819868824130076	33.0	33.0	33.0	27.0	33.0
6	34.033799823702424	37.0	33.0	37.0	27.0	37.0
7	35.44447499105421	37.0	37.0	37.0	33.0	37.0
8	35.19160579163721	37.0	37.0	37.0	33.0	37.0
9	35.75992546627218	37.0	37.0	37.0	33.0	37.0
10-11	35.838644079630654	37.0	37.0	37.0	33.0	37.0
12-13	35.96012249190515	37.0	37.0	37.0	33.0	37.0
14-15	35.99996727148954	37.0	37.0	37.0	33.0	37.0
16-17	36.06002627008439	37.0	37.0	37.0	37.0	37.0
18-19	36.089073914068024	37.0	37.0	37.0	37.0	37.0
20-21	36.11387012454289	37.0	37.0	37.0	37.0	37.0
22-23	36.11266680630831	37.0	37.0	37.0	37.0	37.0
24-25	36.115315633754875	37.0	37.0	37.0	37.0	37.0
26-27	36.06833822079089	37.0	37.0	37.0	37.0	37.0
28-29	36.04394457099468	37.0	37.0	37.0	37.0	37.0
30-31	35.98858211365084	37.0	37.0	37.0	37.0	37.0
32-33	35.925098621911516	37.0	37.0	37.0	35.0	37.0
34-35	35.80986917323419	37.0	37.0	37.0	33.0	37.0
36-37	35.71072360554726	37.0	37.0	37.0	33.0	37.0
38-39	35.690039186936524	37.0	37.0	37.0	33.0	37.0
40-41	35.58326787631241	37.0	37.0	37.0	33.0	37.0
42-43	35.538554185321914	37.0	37.0	37.0	33.0	37.0
44-45	35.447686748880685	37.0	37.0	37.0	33.0	37.0
46-47	35.317804746070394	37.0	37.0	37.0	33.0	37.0
48-49	35.16653902547587	37.0	37.0	37.0	33.0	37.0
50-51	35.07718473716824	37.0	37.0	37.0	33.0	37.0
52-53	34.98521980467625	37.0	37.0	37.0	27.0	37.0
54-55	34.92425749919269	37.0	37.0	37.0	27.0	37.0
56-57	34.921470121051854	37.0	37.0	37.0	27.0	37.0
58-59	34.87926452491294	37.0	37.0	37.0	27.0	37.0
60-61	34.84360353991569	37.0	37.0	37.0	27.0	37.0
62-63	34.81721454193177	37.0	37.0	37.0	27.0	37.0
64-65	34.7757966119446	37.0	37.0	37.0	27.0	37.0
66-67	34.754451077422566	37.0	37.0	37.0	27.0	37.0
68-69	34.73490561097583	37.0	37.0	37.0	27.0	37.0
70-71	34.61666404838583	37.0	37.0	37.0	27.0	37.0
72-73	34.60573599874323	37.0	37.0	37.0	27.0	37.0
74-75	34.52551623770499	37.0	37.0	37.0	27.0	37.0
76-77	34.417266689358435	37.0	37.0	37.0	27.0	37.0
78-79	34.403627628099386	37.0	37.0	37.0	27.0	37.0
80-81	34.38875142914495	37.0	37.0	37.0	27.0	37.0
82-83	34.31031537192679	37.0	37.0	37.0	27.0	37.0
84-85	34.22244041229196	37.0	37.0	37.0	27.0	37.0
86-87	34.0820503757233	37.0	37.0	37.0	27.0	37.0
88-89	33.9934412065038	37.0	37.0	37.0	27.0	37.0
90-91	33.95800713917908	37.0	37.0	37.0	24.5	37.0
92-93	33.78178811125947	37.0	35.0	37.0	22.0	37.0
94-95	33.840617608811385	37.0	37.0	37.0	22.0	37.0
96-97	33.826437436179404	37.0	37.0	37.0	22.0	37.0
98-99	33.7905778982187	37.0	37.0	37.0	22.0	37.0
100-101	33.665731722217856	37.0	33.0	37.0	22.0	37.0
102-103	33.58388426325941	37.0	33.0	37.0	22.0	37.0
104-105	33.44082467118757	37.0	33.0	37.0	22.0	37.0
106-107	33.39468728999206	37.0	33.0	37.0	18.0	37.0
108-109	33.276375906579744	37.0	33.0	37.0	14.0	37.0
110-111	33.201097059670616	37.0	33.0	37.0	14.0	37.0
112-113	33.04856801857234	37.0	33.0	37.0	14.0	37.0
114-115	33.03006004590719	37.0	33.0	37.0	14.0	37.0
116-117	32.87956999101057	37.0	33.0	37.0	14.0	37.0
118-119	32.741337854231574	37.0	33.0	37.0	14.0	37.0
120-121	32.64381234781243	37.0	33.0	37.0	14.0	37.0
122-123	32.573150402778865	37.0	33.0	37.0	14.0	37.0
124-125	31.215696157236493	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	1.0
17	11.0
18	62.0
19	339.0
20	1230.0
21	2774.0
22	4328.0
23	5683.0
24	5201.0
25	4067.0
26	3975.0
27	4477.0
28	5958.0
29	7489.0
30	9731.0
31	13139.0
32	18758.0
33	27676.0
34	45214.0
35	91314.0
36	206886.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	45.065264692467004	16.619635931917827	12.668181252741642	25.646918122873526
2	17.698269316366876	20.867480079246633	37.688407125214916	23.745843479171576
3	20.714310650293683	25.211426177571806	28.60602728248632	25.468235889648188
4	14.75675958735534	17.4488121628934	34.86860479324129	32.92582345650998
5	15.93986274916264	34.46828938324405	32.432001920202495	17.159845947390817
6	29.993977770116647	33.933956212279234	18.858525601022908	17.213540416581203
7	26.049494235418358	32.08419518410878	22.62980127248449	19.236509307988374
8	25.951090513968527	31.6609064488257	21.602344234109218	20.785658803096556
9	27.22793880204924	16.0476614388326	20.85853428638756	35.865865472730604
10-11	25.48787299592421	25.180006807530177	26.68497281351731	22.647147383028305
12-13	26.811770494342866	23.973550970892322	26.80500659480205	22.409671939962756
14-15	24.904214559386972	24.171968685361193	25.801193936061583	25.122622819190255
16-17	23.469942135993506	26.3652152663228	26.61133366498224	23.553508932701455
18-19	23.43001335323227	25.008291222649877	29.05364421054469	22.508051213573168
20-21	24.755597981726442	23.839956361652803	29.02801036410746	22.376435292513296
22-23	24.61118529573482	23.616020387680116	28.818108030267325	22.954686286317738
24-25	23.787081402351216	24.332229291580482	28.84199984290315	23.038689463165152
26-27	23.433722584417737	24.332883861789682	29.053753305579555	23.179640248213023
28-29	23.329507667332887	24.390693594700508	29.3697688017665	22.91002993620011
30-31	23.997573710542795	24.09499621437751	29.27257136498419	22.6348587100955
32-33	23.366134645385817	24.24326063951649	29.112291764397845	23.278312950699846
34-35	23.392266471168362	24.391467895513138	29.17703069497901	23.039234938339487
36-37	23.578004771894914	24.473035084446394	28.84389008713563	23.105070056523065
38-39	23.523725988182825	24.376412780701525	28.82912662878887	23.27073460232678
40-41	23.227880001832833	24.47169691931391	28.943044727660716	23.357378351192544
42-43	23.629286258988174	24.296846788367272	28.710775773193063	23.363091179451487
44-45	23.555254453259323	24.392667940896672	28.68512118276473	23.366956423079273
46-47	23.32888225591077	24.37193988427199	28.650210771607366	23.648967088209883
48-49	23.380047827263287	24.798719660670805	28.390782778694174	23.430449733371734
50-51	23.298881121322406	24.93977954075354	28.29892475933635	23.462414578587698
52-53	23.295608270276404	24.85184894265092	28.020950610495817	23.83159217657686
54-55	23.07327258921792	24.653514169263126	28.11924523691078	24.153968004608174
56-57	23.390193665505894	24.60954887021182	27.701193063301304	24.29906440098098
58-59	23.34677384162892	24.696170327896034	28.05880658759459	23.89824924288046
60-61	23.400666788853105	24.678278742177888	28.334707930772655	23.586346538196352
62-63	23.001488056275583	24.753008841061625	28.374636713533896	23.8708663891289
64-65	22.9846874209061	24.420923554927167	28.516896639000166	24.077492385166565
66-67	23.15127553914766	24.584020632052994	28.26859633964339	23.996107489155953
68-69	23.115601462746227	24.51354523952906	28.323689332251114	24.047163965473604
70-71	22.99526963928818	24.72137128095026	28.409547124691258	23.873811955070302
72-73	23.24607912444689	24.808429118773947	28.41707468209707	23.5284170746821
74-75	23.282832418857314	24.58526465208965	28.59662053339885	23.535282395654193
76-77	23.10302646381022	24.39519872431984	28.454111556482705	24.047663255387235
78-79	23.08071623150428	24.668814386596132	28.555111053411895	23.69535832848769
80-81	22.937776555913388	24.886650258773425	28.48187713280793	23.69369605250526
82-83	22.982505520208765	24.802647081926008	28.51166007732656	23.703187320538667
84-85	23.005120353980367	24.968960106229055	28.056787990700588	23.96913154908999
86-87	22.697652147961573	25.14749915550789	28.23500602896307	23.91984266756746
88-89	22.815814829437517	24.639938229816146	28.252969026321228	24.29127791442511
90-91	23.184892010903756	24.64396798769833	28.109055008038027	24.062084993359896
92-93	23.030118250276203	24.917467404952284	27.937597599369678	24.114816745401836
94-95	22.5091067378405	25.05582956681493	27.96158533059722	24.47347836474735
96-97	22.63580149940216	24.918506008954523	27.75835886157149	24.687333630071826
98-99	22.655984080852512	24.970762530655705	27.583043140540585	24.790210247951194
100-101	22.823663149442744	25.325975964181918	27.318051300849195	24.532309585526143
102-103	23.048071636163694	25.115968022063377	27.59209802843453	24.243862313338397
104-105	23.138344586670332	25.107105803187153	27.418540131810776	24.336009478331736
106-107	23.04556245036176	25.09371263495056	27.446565251922255	24.414159662765428
108-109	23.10874543298419	25.173380229908133	27.18652188949267	24.531352447615006
110-111	22.779697850391432	25.654024734026304	27.14688119114323	24.419396224439033
112-113	22.881374422887266	25.749701079604463	26.890507859206313	24.478416638301958
114-115	22.737805357002593	25.825740318906604	26.990657101214012	24.44579722287679
116-117	22.808062559456793	25.60613201371979	27.0146580088847	24.571147417938715
118-119	22.64562005254017	25.468017699578454	27.1867008788696	24.699661369011775
120-121	22.67782782821004	25.508519786042584	27.42379430763306	24.38985807811431
122-123	22.515142390839507	25.76333795896281	27.323069672453066	24.39844997774461
124-125	22.57620040387114	25.64843573572296	27.178607702789243	24.59675615761666
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	210.0
1	199.5
2	371.5
3	529.0
4	379.5
5	327.5
6	498.5
7	977.0
8	1517.5
9	1685.0
10	1449.5
11	1143.0
12	1001.0
13	941.5
14	945.0
15	917.5
16	920.5
17	906.0
18	861.5
19	862.5
20	869.0
21	865.5
22	841.5
23	828.0
24	905.0
25	1053.0
26	1245.0
27	1454.5
28	1697.0
29	2149.5
30	2873.0
31	3521.5
32	4093.5
33	4749.0
34	5512.0
35	6230.5
36	6969.5
37	8059.0
38	8888.5
39	9377.0
40	9808.0
41	10254.5
42	10870.0
43	11795.5
44	12993.0
45	14086.5
46	15699.5
47	17170.0
48	18585.0
49	19813.0
50	20729.0
51	20806.0
52	19637.0
53	19065.5
54	18716.5
55	18188.5
56	17254.0
57	15511.0
58	13739.5
59	12038.0
60	10311.5
61	8747.0
62	7122.5
63	5490.5
64	4112.5
65	3195.5
66	2554.0
67	2026.0
68	1665.5
69	1390.5
70	1176.5
71	993.5
72	805.0
73	764.5
74	655.5
75	520.5
76	415.5
77	287.5
78	201.5
79	122.5
80	92.5
81	74.0
82	49.0
83	27.5
84	14.0
85	9.0
86	6.0
87	4.5
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02290995732202236
2	0.0
3	0.0
4	8.727602789341852E-4
5	0.0067638921617399345
6	0.003054660976269648
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.0909503486677315E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.63665244067412E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0017455205578683704
30-31	6.545702092006388E-4
32-33	2.181900697335463E-4
34-35	0.0
36-37	0.0016364255230015973
38-39	0.0
40-41	0.0019637106276019166
42-43	0.0012000453835345046
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.08803969313748593
76-77	0.11367702633117761
78-79	1.0909503486677315E-4
80-81	0.0
82-83	0.0
84-85	0.0954581555084265
86-87	0.20520776058440027
88-89	0.24819120432190891
90-91	0.10734951430890478
92-93	0.16789725865996386
94-95	3.272851046003194E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0016364255230015973
106-107	0.0
108-109	5.454751743338657E-4
110-111	0.0
112-113	0.0
114-115	0.0
116-117	0.0
118-119	0.0
120-121	1.0909503486677315E-4
122-123	0.0
124-125	3.272851046003194E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	458316.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.30319828125963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.83124974299733	65.38670816406503
2	9.18364778229795	13.831160997912653
3	1.982529754643274	4.478724936371983
4	0.7303929379997299	2.2000369693370168
5	0.3831064614473432	1.4424570914600516
6	0.2034458910785365	0.919207576523675
7	0.14143639630714025	0.7455429096712378
8	0.09574892380197275	0.5768160155421739
9	0.06190567151515675	0.4195525551156337
>10	0.3464702620575451	4.814813026809124
>50	0.02627290221866485	1.3952902711186428
>100	0.011822805973252356	1.7051624253766913
>500	9.85233831104363E-4	0.4649318868322232
>1k	9.85233831104363E-4	1.619595173863952
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2695	0.5880222379319072	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2363	0.5155831347803699	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1491	0.3253213939727175	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	699	0.15251485874374884	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	610	0.13309594253746324	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	571	0.12458652981785492	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.181900697335463E-4	0.0
2	0.0	0.0	0.0	2.181900697335463E-4	0.0
3	0.0	0.0	0.0	2.181900697335463E-4	0.0
4	0.0	0.0	0.0	2.181900697335463E-4	0.0
5	0.0	0.0	0.0	2.181900697335463E-4	0.0
6	0.0	0.0	0.0	2.181900697335463E-4	0.0
7	0.0	0.0	0.0	2.181900697335463E-4	0.0
8	0.0	0.0	0.0	2.181900697335463E-4	0.0
9	0.0	0.0	0.0	2.181900697335463E-4	0.0
10-11	0.0	0.0	0.0	4.363801394670926E-4	0.0
12-13	0.0	0.0	0.0	6.545702092006388E-4	0.0
14-15	0.0	0.0	0.0	7.636652440674121E-4	1.0909503486677315E-4
16-17	0.0	0.0	0.0	8.727602789341852E-4	2.181900697335463E-4
18-19	0.0	0.0	0.0	8.727602789341852E-4	2.181900697335463E-4
20-21	0.0	0.0	0.0	0.0010909503486677314	2.181900697335463E-4
22-23	0.0	0.0	0.0	0.0033819460808699672	2.181900697335463E-4
24-25	0.0	0.0	0.0	0.008400317684741532	2.181900697335463E-4
26-27	0.0	0.0	0.0	0.014727829707014374	2.181900697335463E-4
28-29	0.0	0.0	0.0	0.02498276298449105	2.181900697335463E-4
30-31	0.0	0.0	0.0	0.04734724513217954	2.181900697335463E-4
32-33	0.0	0.0	0.0	0.0877124080328856	2.181900697335463E-4
34-35	0.0	0.0	0.0	0.12785938086385812	2.181900697335463E-4
36-37	0.0	0.0	0.0	0.1876434599708498	2.181900697335463E-4
38-39	0.0	0.0	0.0	0.26510093472625873	2.181900697335463E-4
40-41	0.0	0.0	0.0	0.35695895408408174	2.181900697335463E-4
42-43	0.0	0.0	0.0	0.43899842030389513	2.181900697335463E-4
44-45	0.0	0.0	0.0	0.5247471177091788	2.181900697335463E-4
46-47	0.0	0.0	0.0	0.6143141413347996	2.181900697335463E-4
48-49	0.0	0.0	0.0	0.7177362343885005	2.181900697335463E-4
50-51	0.0	0.0	0.0	0.8071941629792545	2.181900697335463E-4
52-53	0.0	0.0	0.0	0.9043978390455494	2.181900697335463E-4
54-55	0.0	0.0	0.0	1.0005105647631765	2.181900697335463E-4
56-57	0.0	0.0	0.0	1.0944413897834682	2.181900697335463E-4
58-59	0.0	0.0	0.0	1.2008090487785719	2.181900697335463E-4
60-61	0.0	0.0	0.0	1.2999764354724688	2.181900697335463E-4
62-63	0.0	0.0	0.0	1.4012166278288343	4.363801394670926E-4
64-65	0.0	0.0	0.0	1.4908927464893218	8.727602789341852E-4
66-67	0.0	0.0	0.0	1.597042215414692	8.727602789341852E-4
68-69	0.0	0.0	0.0	1.7198832246746787	8.727602789341852E-4
70-71	0.0	0.0	0.0	1.8512336466542734	8.727602789341852E-4
72-73	0.0	0.0	0.0	1.9748383211583274	8.727602789341852E-4
74-75	0.0	0.0	0.0	2.088624442524372	8.727602789341852E-4
76-77	0.0	0.0	0.0	2.215720158144163	8.727602789341852E-4
78-79	0.0	0.0	0.0	2.3576527985058346	8.727602789341852E-4
80-81	0.0	0.0	0.0	2.501658244529975	8.727602789341852E-4
82-83	0.0	0.0	0.0	2.664755321655801	8.727602789341852E-4
84-85	0.0	0.0	0.0	2.822288552003421	8.727602789341852E-4
86-87	0.0	0.0	0.0	2.9812400178043097	9.818553138009583E-4
88-89	0.0	0.0	0.0	3.156337548765481	0.0010909503486677314
90-91	0.0	0.0	0.0	3.341253632864661	0.0010909503486677314
92-93	0.0	0.0	0.0	3.5161329737560987	0.0010909503486677314
94-95	0.0	0.0	0.0	3.6999581075066112	0.0013091404184012777
96-97	0.0	0.0	0.0	3.8843287164314577	0.0013091404184012777
98-99	0.0	0.0	0.0	4.088554621702057	0.0013091404184012777
100-101	0.0	0.0	0.0	4.309472067307272	0.0013091404184012777
102-103	0.0	0.0	0.0	4.531807748365756	0.0013091404184012777
104-105	0.0	0.0	0.0	4.750543293273637	0.0013091404184012777
106-107	0.0	0.0	0.0	4.994697981305475	0.0013091404184012777
108-109	0.0	0.0	0.0	5.236998053744578	0.0013091404184012777
110-111	0.0	0.0	0.0	5.490753104844693	0.0013091404184012777
112-113	0.0	0.0	0.0	5.745271821188874	0.0013091404184012777
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	60	2.1967531E-5	49.56251	4
GTATCAA	3985	0.0	34.032074	1
GGTATCA	2905	0.0	32.96565	1
TATAACG	85	0.007220707	27.98824	2
TATCAAC	4910	0.0	27.496595	2
ATCAACG	4990	0.0	26.936577	3
TCAACGC	5115	0.0	26.510857	4
CAACGCA	5100	0.0	26.472212	5
AACGCAG	5265	0.0	25.975819	6
GTATAAC	145	1.3411859E-4	24.613033	1
ACGCAGA	5630	0.0	23.974922	7
GAGTACT	2480	0.0	23.856743	12-13
CGCAGAG	5730	0.0	23.556513	8
GCAGAGT	6080	0.0	22.396063	9
TTACCCT	160	2.6153645E-4	22.303131	4
ACCGACC	160	2.6192298E-4	22.298264	8
CCTATAC	135	0.0025591943	22.027782	3
GTACTTT	2715	0.0	21.57278	14-15
CATAGGG	140	0.0031604902	21.241076	2
AGTACTT	2540	0.0	20.718071	12-13
>>END_MODULE