ERR1142555_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117924
Sequences flagged as poor quality	0
Sequence length	125
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1104	0.9361961941589497	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	962	0.8157796546928531	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	403	0.34174553101997895	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	356	0.30188935246429904	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	254	0.21539296496048302	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	247	0.20945693836708387	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	227	0.1924968623859435	No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA	215	0.18232081679725926	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	195	0.16536074081611885	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	191	0.16196872561989079	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	185	0.15688070282554867	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	179	0.15179268003120655	No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTG	165	0.13992062684440826	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	165	0.13992062684440826	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA	158	0.13398460025100914	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	157	0.1331365964519521	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	156	0.13228859265289508	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	155	0.13144058885383808	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	154	0.13059258505478105	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	154	0.13059258505478105	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	149	0.12635256605949596	No Hit
TATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAA	144	0.12211254706421083	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	141	0.11956853566703977	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	141	0.11956853566703977	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	138	0.11702452426986872	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	138	0.11702452426986872	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	137	0.11617652047081171	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	137	0.11617652047081171	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	133	0.11278450527458363	No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG	133	0.11278450527458363	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	127	0.10769648248024151	No Hit
GGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTG	124	0.10515247108307045	No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT	121	0.10260845968589939	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	120	0.10176045588684236	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	119	0.10091245208778536	No Hit

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	15	2.70091E-4	118.94148	1
GTCAACG	25	1.7234754E-5	95.153175	1
GCGCAGA	45	4.0381565E-10	92.51004	1
GTCATAC	25	0.0020562666	71.36488	3
GGTATCA	825	0.0	61.272877	1
CGTAATC	15	0.004240635	59.47074	12-13
CGCAACT	15	0.004240635	59.47074	24-25
CGTCATA	30	0.004235383	59.47074	2
TGGTACA	30	0.004235383	59.47074	4
GTCAGAT	35	0.007794154	50.97492	1
TACAGAT	35	0.007794154	50.97492	7
TATTCTC	75	1.4644793E-6	47.576588	5
TCTCTGC	85	3.4659915E-6	41.979343	8
GTATCAA	1245	0.0	40.602512	1
GAATCAT	60	0.0013069011	39.64716	6
TAATAAT	85	1.7061338E-4	34.982784	3
TTCTGGC	85	1.7061338E-4	34.982784	9
TATCAAC	1455	0.0	34.742355	2
CACGCCT	35	0.0027912378	33.98328	32-33
GTGTCTA	35	0.0027912378	33.98328	60-61

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content