ERR1142554_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142554_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	140576
Sequences flagged as poor quality	0
Sequence length	125
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	335	0.2383052583655816	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	302	0.21483041201912134	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	296	0.21056225813794674	No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA	235	0.16716936034600502	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	226	0.16076712952424313	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	222	0.15792169360346006	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	220	0.15649897564306853	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	208	0.14796266788071932	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	205	0.14582859094013204	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	203	0.1444058729797405	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	201	0.14298315501934897	No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTG	199	0.14156043705895743	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	191	0.1358695652173913	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	186	0.13231277031641248	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA	182	0.1294673343956294	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	178	0.12662189847484634	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	175	0.12448782153425905	No Hit
TATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAA	174	0.12377646255406329	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	172	0.12235374459367175	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	171	0.12164238561347598	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	167	0.11879694969269292	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	164	0.11666287275210563	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	162	0.11524015479171408	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	162	0.11524015479171408	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	161	0.11452879581151831	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	160	0.11381743683132256	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	158	0.11239471887093103	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	154	0.10954928295014796	No Hit
GGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTG	154	0.10954928295014796	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	153	0.10883792396995219	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	153	0.10883792396995219	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	150	0.1067038470293649	No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG	146	0.10385841110858185	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	145	0.10314705212838607	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	142	0.10101297518779877	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	25	1.7183942E-5	95.222534	1
GGGTAAA	30	0.0042248503	59.514084	1
ACGCAAG	35	0.007774797	51.01207	8
TAACAGT	50	5.310307E-4	47.611267	1
AACAGTT	55	8.492866E-4	43.282967	2
AAAACGC	60	0.0013030731	39.676056	5
GAACAAA	60	0.0013030731	39.676056	1
TCTTGGC	85	1.7004854E-4	35.008286	8
AGTTCTT	70	0.0027774626	34.008045	5
GCTTTGA	70	0.0027774626	34.008045	1
GAGTACT	45	2.3850898E-4	33.063377	12-13
TACAAAA	90	2.37904E-4	33.063377	2
AGGGCTA	220	3.6379788E-12	32.462223	5
CAGTTCT	80	0.005340178	29.757042	4
TTTGATA	80	0.005340178	29.757042	2
ACGCCAC	60	3.68919E-5	29.746437	40-41
GTATCAA	1015	0.0	28.144588	1
TGGGGGA	405	0.0	27.920189	6
CTATTGA	235	3.3469405E-10	27.857658	9
TCTTACC	150	5.241376E-6	27.773237	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content