FastQCFastQC Report
Sun 1 Jan 2017
ERR1142553_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142553_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513454
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10980.2138458362384946No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9180.1787891417731676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.038.9273031
GTATCAA21600.032.549761
TATCAAC27300.026.1875952
CAACGCA27200.026.067395
ATCAACG27550.025.9524963
AACGCAG29200.024.6852256
TCAACGC28500.024.6692924
ACGCAGA31800.022.4796057
TGTGTCG1400.00312802321.2795035
CGCAGAG33050.021.2689028
GCAGAGT34500.020.2023229
ATCTACG1500.004664798319.8608685
TAGACAG2408.748633E-619.8608685
AGAGTAC32150.019.26740510-11
CAGAGTA34050.018.89198110-11
GTTAGAC1600.006773148718.6195643
GTACATG42500.018.0850751
TACATGG42900.017.636952
GAGTACA36300.017.55604712-13
ACATGGG42150.017.3870943
Produced by FastQC (version 0.11.5)