##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142553_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513454
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.880238541329895	33.0	33.0	33.0	27.0	33.0
2	30.970571852590496	33.0	33.0	33.0	27.0	33.0
3	31.08628231545572	33.0	33.0	33.0	27.0	33.0
4	31.03006695828643	33.0	33.0	33.0	27.0	33.0
5	31.0221986779731	33.0	33.0	33.0	27.0	33.0
6	34.13356405831876	37.0	37.0	37.0	27.0	37.0
7	34.22475820618789	37.0	37.0	37.0	27.0	37.0
8	34.24044802455526	37.0	37.0	37.0	27.0	37.0
9	34.20072099934951	37.0	37.0	37.0	27.0	37.0
10-11	34.24187171586939	37.0	37.0	37.0	27.0	37.0
12-13	34.242690679203974	37.0	37.0	37.0	27.0	37.0
14-15	34.21382733409419	37.0	37.0	37.0	27.0	37.0
16-17	34.18935678756033	37.0	37.0	37.0	27.0	37.0
18-19	34.18359483030612	37.0	37.0	37.0	27.0	37.0
20-21	34.26589431575175	37.0	37.0	37.0	27.0	37.0
22-23	34.31365029778715	37.0	37.0	37.0	27.0	37.0
24-25	34.35306376033685	37.0	37.0	37.0	27.0	37.0
26-27	34.32948715951186	37.0	37.0	37.0	27.0	37.0
28-29	34.35444168318875	37.0	37.0	37.0	27.0	37.0
30-31	34.42343423169359	37.0	37.0	37.0	27.0	37.0
32-33	34.434242405356656	37.0	37.0	37.0	27.0	37.0
34-35	34.430518605366785	37.0	37.0	37.0	27.0	37.0
36-37	34.37416691660792	37.0	37.0	37.0	27.0	37.0
38-39	34.35568522204521	37.0	37.0	37.0	27.0	37.0
40-41	34.33467847168393	37.0	37.0	37.0	27.0	37.0
42-43	34.32739446961169	37.0	37.0	37.0	27.0	37.0
44-45	34.344937423800374	37.0	37.0	37.0	27.0	37.0
46-47	34.33382445165487	37.0	37.0	37.0	27.0	37.0
48-49	34.294706049616906	37.0	37.0	37.0	27.0	37.0
50-51	34.28211680111558	37.0	37.0	37.0	27.0	37.0
52-53	34.23286117159473	37.0	37.0	37.0	27.0	37.0
54-55	34.18778021010645	37.0	37.0	37.0	27.0	37.0
56-57	34.107146891445	37.0	37.0	37.0	27.0	37.0
58-59	34.057508559676236	37.0	37.0	37.0	27.0	37.0
60-61	34.02020044638857	37.0	37.0	37.0	27.0	37.0
62-63	34.07177176533828	37.0	37.0	37.0	27.0	37.0
64-65	34.005946004900146	37.0	37.0	37.0	27.0	37.0
66-67	33.97076369061298	37.0	37.0	37.0	27.0	37.0
68-69	33.93124797937108	37.0	37.0	37.0	27.0	37.0
70-71	33.89415799662677	37.0	37.0	37.0	27.0	37.0
72-73	33.84697558106471	37.0	37.0	37.0	27.0	37.0
74-75	33.75845353235149	37.0	35.0	37.0	24.5	37.0
76-77	33.70171232476522	37.0	33.0	37.0	22.0	37.0
78-79	33.61614282876363	37.0	33.0	37.0	22.0	37.0
80-81	33.536200906020795	37.0	33.0	37.0	22.0	37.0
82-83	33.49154549385145	37.0	33.0	37.0	22.0	37.0
84-85	33.440784374062716	37.0	33.0	37.0	18.0	37.0
86-87	33.361073241225114	37.0	33.0	37.0	14.0	37.0
88-89	33.29817179338363	37.0	33.0	37.0	14.0	37.0
90-91	33.197978786804654	37.0	33.0	37.0	14.0	37.0
92-93	33.05729140292996	37.0	33.0	37.0	14.0	37.0
94-95	32.95967506339419	37.0	33.0	37.0	14.0	37.0
96-97	32.85198576698205	37.0	33.0	37.0	14.0	37.0
98-99	32.78584936527907	37.0	33.0	37.0	14.0	37.0
100-101	32.64570828155979	37.0	33.0	37.0	14.0	37.0
102-103	32.561620904696426	37.0	33.0	37.0	14.0	37.0
104-105	32.430709469592216	37.0	33.0	37.0	14.0	37.0
106-107	32.32388685257102	37.0	33.0	37.0	14.0	37.0
108-109	32.17325505303299	37.0	33.0	37.0	14.0	37.0
110-111	32.09421194498436	37.0	33.0	37.0	14.0	37.0
112-113	31.97358867590865	37.0	33.0	37.0	14.0	37.0
114-115	31.77441017111562	37.0	27.0	37.0	14.0	37.0
116-117	31.596096242311873	37.0	27.0	37.0	14.0	37.0
118-119	31.47382628239336	37.0	27.0	37.0	14.0	37.0
120-121	31.350388739789736	37.0	27.0	37.0	14.0	37.0
122-123	31.158663677758867	37.0	27.0	37.0	14.0	37.0
124-125	29.49123582638367	35.0	20.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	918.0
1	71.0
2	34.0
3	18.0
4	24.0
5	22.0
6	16.0
7	15.0
8	13.0
9	11.0
10	19.0
11	9.0
12	8.0
13	10.0
14	33.0
15	183.0
16	249.0
17	593.0
18	1573.0
19	3015.0
20	4247.0
21	5110.0
22	5871.0
23	6455.0
24	7408.0
25	8271.0
26	9434.0
27	10743.0
28	12472.0
29	14590.0
30	17326.0
31	20428.0
32	25309.0
33	32994.0
34	46866.0
35	82683.0
36	196413.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.34792892468284	19.623316457180838	12.406708759309575	24.62204585882675
2	17.856493954318918	22.703197988484973	35.64324132123872	23.797066735957394
3	20.21065873831356	26.52846744986502	28.282459300122287	24.97841451169914
4	15.087225771161773	19.13438434038563	33.506222039891384	32.27216784856121
5	16.014811341032104	36.72606835101706	30.93792133184829	16.321198976102547
6	29.690092294223746	35.105807129889996	18.373644068293427	16.830456507592828
7	26.545532003040744	32.70772712260879	21.358094415467924	19.388646458882548
8	25.770091357662807	33.313400752357225	20.3916165909424	20.52489129903757
9	26.413055161968046	18.106610641520497	19.893487076757708	35.58684711975375
10-11	25.522939778013807	26.671760812275195	25.317740818079397	22.487558591631597
12-13	26.277315916810306	25.57582792975544	25.066347728543008	23.080508424891242
14-15	24.717552075649046	25.885449693833586	23.38824168533694	26.008756545180432
16-17	24.308340153088032	27.788661173101975	23.985581834126638	23.917416839683355
18-19	24.303915144685458	26.889142999993165	25.372418702620674	23.434523152700702
20-21	26.15238913514675	25.496768272441468	24.906758313643554	23.444084278768234
22-23	26.105990355992553	25.27368459077405	24.622582285994906	23.997742767238492
24-25	25.064452928618074	25.987830818505824	24.809667402388346	24.138048850487753
26-27	24.41153730509858	26.32374819888243	24.897594995528934	24.367119500490062
28-29	24.321725761975646	26.025915712926135	25.286610214988166	24.365748310110057
30-31	24.79355219078706	25.732377263605056	25.20107919006083	24.272991355547052
32-33	24.446925869276846	26.068105350800046	24.76522716912917	24.71974161079394
34-35	24.710218919673483	25.972526195975377	24.94879899726082	24.368455887090317
36-37	24.78239436963429	26.05566916720761	24.730073356793188	24.431863106364908
38-39	24.579039469228135	26.050607980902207	24.90911273534312	24.461239814526543
40-41	24.35832982720566	26.016768889405185	25.16573164505558	24.459169638333574
42-43	24.50235717841352	26.086693410637075	24.979063570612865	24.431885840336545
44-45	24.5785994598968	26.137910774639156	24.8406480848003	24.44284168066374
46-47	24.60572780529132	26.099462591235532	24.82172591567387	24.47308368779928
48-49	24.621288648273023	26.147162824653304	24.91399023807502	24.317558288998654
50-51	24.37748017325346	26.383773201148518	24.78640987052837	24.452336755069652
52-53	24.355191758326516	26.148197036293592	24.920674283237233	24.575936922142663
54-55	24.289840811493356	25.88543542796005	25.1452825749744	24.679441185572195
56-57	24.43464135869597	25.87500512498121	24.761468731757507	24.928884784565312
58-59	24.370066173190438	25.91618973617601	25.062742024124564	24.651002066508987
60-61	24.35761880354397	26.06214151472993	25.144321593322104	24.435918088403994
62-63	24.106795983827546	26.0972143318711	25.046512399727465	24.749477284573892
64-65	24.16303277192222	25.853611393394853	25.128003881360776	24.855351953322145
66-67	24.52865183785272	25.758344868518414	24.927713924806863	24.785289368822006
68-69	24.322583164141808	25.70660237388724	24.942312197407464	25.028502264563485
70-71	24.3491018273857	25.672226294792715	25.020864467348453	24.95780741047313
72-73	24.55222620373525	25.804889809918812	25.04517027068974	24.597713715656198
74-75	24.329547351130085	25.889129965868573	25.16365418044075	24.617668502560587
76-77	24.36865285277547	25.512847543524693	25.119378101585593	24.999121502114253
78-79	24.24626389964047	25.787072785258058	25.086953593187015	24.879709721914452
80-81	24.11541070652943	25.951987037769094	25.20070472369853	24.731897532002947
82-83	24.315467009258573	25.783327541030975	25.332211318809623	24.568994130900826
84-85	24.244784422809456	25.91425713100554	24.98782421979845	24.85313422638655
86-87	24.022118470054572	26.11226704558763	25.103448545084046	24.762165939273753
88-89	24.291496395139905	25.615832299585495	25.113777329251047	24.978893976023556
90-91	24.44411696965675	25.71261549992388	25.11515835249386	24.72810917792551
92-93	24.371483568185102	25.861708815043006	25.061982906383363	24.704824710388532
94-95	23.84056386853478	25.829660484308576	25.3780854859677	24.95169016118895
96-97	23.96430244359435	25.81807713326567	25.172242173471776	25.045378249668204
98-99	23.7635114761553	25.922225030421252	25.175818739101285	25.13844475432216
100-101	24.118948748770627	26.095313548870536	24.922042087515806	24.86369561484303
102-103	24.011108682415024	26.093205982919326	25.323092476073008	24.57259285859264
104-105	23.939470227884367	26.222412174649545	25.174713949208545	24.663403648257546
106-107	23.97393412100844	26.070465305994993	25.274690960471823	24.680909612524736
108-109	24.03314243224703	26.15406683717331	25.06759928141305	24.745191449166608
110-111	23.775716108988295	26.567848374972193	24.965750885012746	24.69068463102677
112-113	23.8721821018644	26.55564369999795	24.845706568777548	24.726467629360098
114-115	23.780127270704906	26.494825846763888	25.01448919018599	24.71055769234522
116-117	23.8081158775417	26.6052306485325	24.84899580993866	24.737657663987136
118-119	23.73701355400088	26.297669000625355	25.06685294109039	24.898464504283368
120-121	23.76657069235175	26.39729463659363	25.15554766339456	24.68058700766006
122-123	23.809166062562138	26.57237107206032	25.130228024661122	24.488234840716416
124-125	23.727080621967094	26.4698938347678	24.878488516164783	24.924537027100328
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1470.0
1	913.5
2	281.5
3	175.0
4	140.0
5	118.0
6	100.0
7	105.0
8	105.0
9	101.0
10	102.0
11	90.5
12	80.0
13	82.5
14	83.0
15	85.0
16	100.0
17	123.5
18	134.5
19	144.0
20	174.5
21	222.0
22	282.5
23	350.5
24	446.0
25	595.5
26	791.5
27	1058.0
28	1389.5
29	1934.0
30	2662.0
31	3368.5
32	4277.5
33	5193.0
34	6091.5
35	7092.5
36	8092.5
37	9288.5
38	10307.5
39	11029.0
40	11663.5
41	12192.0
42	13021.5
43	14240.0
44	15638.5
45	17127.5
46	18812.5
47	20314.5
48	21684.0
49	22490.5
50	22526.0
51	22288.0
52	21890.0
53	21951.5
54	21673.5
55	20714.0
56	19804.5
57	18242.5
58	16400.5
59	14622.5
60	12765.0
61	10906.5
62	8915.5
63	7044.0
64	5479.0
65	4251.0
66	3317.5
67	2696.5
68	2221.0
69	1847.5
70	1528.0
71	1327.0
72	1120.5
73	977.0
74	828.0
75	685.0
76	543.5
77	379.0
78	277.5
79	198.5
80	128.0
81	86.0
82	61.5
83	46.0
84	33.5
85	16.5
86	8.0
87	6.0
88	4.0
89	2.5
90	0.5
91	0.5
92	1.0
93	1.5
94	1.0
95	1.0
96	1.5
97	1.0
98	0.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36692673540375575
2	0.31180982132771384
3	0.2991504594374569
4	0.30460372302095223
5	0.3279748526645035
6	0.3351809509712652
7	0.3382971015904054
8	0.3367390262808353
9	0.3464769969656484
10-11	0.3419001507437862
12-13	0.34199753045063436
14-15	0.3354730900918096
16-17	0.2845435034102373
18-19	0.27840858187880513
20-21	0.26175665200777476
22-23	0.2575693246133052
24-25	0.24510472213674447
26-27	0.247733974221644
28-29	0.23692482676150153
30-31	0.2372169658820459
32-33	0.23439295438345012
34-35	0.2448125830162001
36-37	0.23984621796694544
38-39	0.2227073895616745
40-41	0.24452044389565572
42-43	0.23156894288485436
44-45	0.2229021489753707
46-47	0.23001086757528424
48-49	0.2254340213534221
50-51	0.22222049102743383
52-53	0.25805622314754584
54-55	0.24588375979152952
56-57	0.2448125830162001
58-59	0.241014774449123
60-61	0.2564007681311276
62-63	0.23858028177791973
64-65	0.24588375979152952
66-67	0.24403354536141506
68-69	0.23643792822726087
70-71	0.2375091050025903
72-73	0.23867766148476785
74-75	0.22777113431777726
76-77	0.23682744705465336
78-79	0.24452044389565572
80-81	0.23302963848757632
82-83	0.24929204953121406
84-85	0.22718685607668845
86-87	0.21852006216720485
88-89	0.22582354018081466
90-91	0.21735150568502729
92-93	0.23682744705465336
94-95	0.2212466939589525
96-97	0.21306679858370953
98-99	0.2075161552933661
100-101	0.1943698948688685
102-103	0.20683449734542916
104-105	0.20352358731259276
106-107	0.20644497851803667
108-109	0.20634759881118853
110-111	0.20099171493454135
112-113	0.20206289170987082
114-115	0.19534369193734977
116-117	0.2046921437947703
118-119	0.18472930389090356
120-121	0.19290919926614652
122-123	0.19232492102505774
124-125	0.18521620242514422
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	513454.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.22288338744838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.64529587888785	70.22735938258991
2	7.78083569833346	12.328404783719346
3	1.748908409426449	4.156607009259583
4	0.6738120256946335	2.135253261466653
5	0.3260634871895498	1.2915844811261237
6	0.1966449860407798	0.9347269678701068
7	0.12332951235720345	0.6839363702993922
8	0.07971687145824094	0.5052320329238748
9	0.06297718171506443	0.4490310530774427
>10	0.33226976587392754	4.912096505070648
>50	0.023102682610061827	1.2586393160898892
>100	0.006480020379764165	0.6671524659979111
>500	2.8174001651148277E-4	0.20489995442826286
>1k	2.8174001651148277E-4	0.24507641608086345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1098	0.2138458362384946	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	918	0.1787891417731676	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.94759413696261E-4	9.73797068481305E-5
22-23	0.0	0.0	0.0	9.73797068481305E-4	2.921391205443915E-4
24-25	0.0	0.0	0.0	0.003700428860228959	3.89518827392522E-4
26-27	0.0	0.0	0.0	0.006524440358824744	3.89518827392522E-4
28-29	1.94759413696261E-4	0.0	0.0	0.012172463356016313	3.89518827392522E-4
30-31	1.94759413696261E-4	0.0	0.0	0.02337112964355132	3.89518827392522E-4
32-33	1.94759413696261E-4	0.0	0.0	0.048008195476128335	3.89518827392522E-4
34-35	1.94759413696261E-4	0.0	0.0	0.07284002072240162	5.84278241088783E-4
36-37	1.94759413696261E-4	0.0	0.0	0.1184137235273267	5.84278241088783E-4
38-39	1.94759413696261E-4	0.0	0.0	0.1743096752581536	5.84278241088783E-4
40-41	1.94759413696261E-4	0.0	0.0	0.24325450770662999	5.84278241088783E-4
42-43	1.94759413696261E-4	0.0	0.0	0.2919443611306953	5.84278241088783E-4
44-45	1.94759413696261E-4	0.0	0.0	0.3482298316889147	5.84278241088783E-4
46-47	1.94759413696261E-4	0.0	0.0	0.4121109193812883	5.84278241088783E-4
48-49	1.94759413696261E-4	0.0	0.0	0.48027671417497964	5.84278241088783E-4
50-51	1.94759413696261E-4	0.0	0.0	0.5426971062646313	5.84278241088783E-4
52-53	1.94759413696261E-4	0.0	0.0	0.6052148780611311	5.84278241088783E-4
54-55	1.94759413696261E-4	0.0	0.0	0.6695828642877454	6.816579479369134E-4
56-57	1.94759413696261E-4	0.0	0.0	0.7356063055307778	7.79037654785044E-4
58-59	1.94759413696261E-4	0.0	0.0	0.8106860595106864	7.79037654785044E-4
60-61	1.94759413696261E-4	0.0	0.0	0.8851815352495063	7.79037654785044E-4
62-63	1.94759413696261E-4	0.0	0.0	0.961819364538985	7.79037654785044E-4
64-65	1.94759413696261E-4	0.0	0.0	1.0335882084860573	7.79037654785044E-4
66-67	1.94759413696261E-4	0.0	0.0	1.1154845419453348	7.79037654785044E-4
68-69	1.94759413696261E-4	0.0	0.0	1.2048791128319187	7.79037654785044E-4
70-71	1.94759413696261E-4	0.0	0.0	1.3010902631978718	7.79037654785044E-4
72-73	1.94759413696261E-4	0.0	0.0	1.393990503530988	0.001168556482177566
74-75	1.94759413696261E-4	0.0	0.0	1.478223949954621	0.001168556482177566
76-77	1.94759413696261E-4	0.0	0.0	1.567715900548053	0.001168556482177566
78-79	1.94759413696261E-4	0.0	0.0	1.678144488113833	0.001168556482177566
80-81	1.94759413696261E-4	0.0	0.0	1.791591846591905	0.0012659361890256966
82-83	1.94759413696261E-4	0.0	0.0	1.9062077615521547	0.001558075309570088
84-85	1.94759413696261E-4	0.0	0.0	2.0202393982713156	0.001558075309570088
86-87	1.94759413696261E-4	0.0	0.0	2.125019962839904	0.001558075309570088
88-89	1.94759413696261E-4	0.0	0.0	2.2513214426219292	0.001558075309570088
90-91	1.94759413696261E-4	0.0	0.0	2.383855223642235	0.001558075309570088
92-93	1.94759413696261E-4	0.0	0.0	2.523400343555606	0.001558075309570088
94-95	1.94759413696261E-4	0.0	0.0	2.6605109707977737	0.001558075309570088
96-97	1.94759413696261E-4	0.0	0.0	2.7971346995057007	0.001558075309570088
98-99	1.94759413696261E-4	0.0	0.0	2.9470994480518216	0.001558075309570088
100-101	1.94759413696261E-4	0.0	0.0	3.105633610800578	0.001558075309570088
102-103	2.921391205443915E-4	0.0	0.0	3.2768271354395915	0.001558075309570088
104-105	3.89518827392522E-4	0.0	0.0	3.4512341904045933	0.001558075309570088
106-107	3.89518827392522E-4	0.0	0.0	3.6232067526983913	0.001558075309570088
108-109	3.89518827392522E-4	0.0	0.0	3.803943488608522	0.001558075309570088
110-111	3.89518827392522E-4	0.0	0.0	3.995976270513035	0.001558075309570088
112-113	3.89518827392522E-4	0.0	0.0	4.197260124568121	0.001558075309570088
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1500	0.0	38.927303	1
GTATCAA	2160	0.0	32.54976	1
TATCAAC	2730	0.0	26.187595	2
CAACGCA	2720	0.0	26.06739	5
ATCAACG	2755	0.0	25.952496	3
AACGCAG	2920	0.0	24.685225	6
TCAACGC	2850	0.0	24.669292	4
ACGCAGA	3180	0.0	22.479605	7
TGTGTCG	140	0.003128023	21.279503	5
CGCAGAG	3305	0.0	21.268902	8
GCAGAGT	3450	0.0	20.202322	9
ATCTACG	150	0.0046647983	19.860868	5
TAGACAG	240	8.748633E-6	19.860868	5
AGAGTAC	3215	0.0	19.267405	10-11
CAGAGTA	3405	0.0	18.891981	10-11
GTTAGAC	160	0.0067731487	18.619564	3
GTACATG	4250	0.0	18.085075	1
TACATGG	4290	0.0	17.63695	2
GAGTACA	3630	0.0	17.556047	12-13
ACATGGG	4215	0.0	17.387094	3
>>END_MODULE