FastQCFastQC Report
Sun 1 Jan 2017
ERR1142553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513454
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.4426881473316013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20660.4023729486964753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.1682721334335695No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.1384739431380416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG1900.075.130951
GGTATCA20150.058.445541
GCGCAGA3150.041.5406571
GTATCAA28550.041.033332
GCGTAAG700.002792460133.9878121
ATCAACG35600.033.2414324
CAACGCA37050.032.100996
TCAACGC37350.032.002375
TATCAAC37950.031.0263063
AACGCAG38900.030.5743377
ACGCAGA42500.027.984518
CGCAGAG44400.026.7869769
CTATTCG900.0095498726.4298159
GTCTTAT1859.2711525E-725.7205071
GAGTACT19500.025.4641112-13
GTGGTAT10300.025.4083651
TGGTATC10400.024.5873532
CAGAGTA46800.023.69788710-11
GTACTTT21400.022.92539614-15
ATGGGTA3154.129106E-1022.6541295
Produced by FastQC (version 0.11.5)